[W::hts_idx_load3] The index file is older than the data file: 1173_CH0_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (6099345) splicing reads. 197268 splicing junctions. 162775 known splicing junctions. 34493 novel splicing junctions.
sampling 10% (12198690) splicing reads. 229430 splicing junctions. 175016 known splicing junctions. 54414 novel splicing junctions.
sampling 15% (18298035) splicing reads. 250194 splicing junctions. 181099 known splicing junctions. 69095 novel splicing junctions.
sampling 20% (24397380) splicing reads. 265514 splicing junctions. 185161 known splicing junctions. 80353 novel splicing junctions.
sampling 25% (30496725) splicing reads. 277389 splicing junctions. 188007 known splicing junctions. 89382 novel splicing junctions.
sampling 30% (36596070) splicing reads. 286880 splicing junctions. 190060 known splicing junctions. 96820 novel splicing junctions.
sampling 35% (42695415) splicing reads. 294676 splicing junctions. 191672 known splicing junctions. 103004 novel splicing junctions.
sampling 40% (48794760) splicing reads. 301257 splicing junctions. 193013 known splicing junctions. 108244 novel splicing junctions.
sampling 45% (54894105) splicing reads. 306689 splicing junctions. 194136 known splicing junctions. 112553 novel splicing junctions.
sampling 50% (60993450) splicing reads. 311200 splicing junctions. 194980 known splicing junctions. 116220 novel splicing junctions.
sampling 55% (67092795) splicing reads. 315139 splicing junctions. 195705 known splicing junctions. 119434 novel splicing junctions.
sampling 60% (73192140) splicing reads. 318366 splicing junctions. 196282 known splicing junctions. 122084 novel splicing junctions.
sampling 65% (79291485) splicing reads. 321227 splicing junctions. 196822 known splicing junctions. 124405 novel splicing junctions.
sampling 70% (85390830) splicing reads. 323545 splicing junctions. 197219 known splicing junctions. 126326 novel splicing junctions.
sampling 75% (91490175) splicing reads. 325571 splicing junctions. 197561 known splicing junctions. 128010 novel splicing junctions.
sampling 80% (97589520) splicing reads. 327169 splicing junctions. 197861 known splicing junctions. 129308 novel splicing junctions.
sampling 85% (103688865) splicing reads. 328450 splicing junctions. 198086 known splicing junctions. 130364 novel splicing junctions.
sampling 90% (109788210) splicing reads. 329451 splicing junctions. 198253 known splicing junctions. 131198 novel splicing junctions.
sampling 95% (115887555) splicing reads. 330129 splicing junctions. 198384 known splicing junctions. 131745 novel splicing junctions.
sampling 100% (121986901) splicing reads. 330498 splicing junctions. 198450 known splicing junctions. 132048 novel splicing junctions.