MultiQC Report

A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

This report has been generated by the nf-core/rnafusion analysis pipeline. For information about how to interpret these results, please see the documentation.

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Report generated on 2026-06-08, 19:47 UTC based on data in: /tmp/nxf.vHeerQZRKY

General Statistics

Showing ⁴⁸⁰/₄₈₀ rows and ⁵⁵/₆₁ columns.

Sample Name	Fragment Length Mean	Fragment Length Median	pct Mito est. counts	pct Ribo est. counts	pct Housekeeping est. counts	pct Male est. counts	pct Mito TPM	pct Ribo TPM	pct Housekeeping TPM	pct Male TPM	ChrY Signature Score	Reads	Frac Unmapped	Frac Dupes	Unique Reads	Frac Reads On Target	Frac Pairs On Target	Frac Reads Off Target	Frac Pairs Off Target	Reads On Target	Pairs On Target	Reads Off Target	Pairs Off Target	Mean Depth	Q50 Depth	Frac Bases Gt30X	Frac Bases Gt100X	N Q30	GC Bias	Unique Reads	Frac Antisense	Total Reads	Expected Distinct	RNA contaminated	RNA contamination %	Frag Length	% Aligned	M Aligned	Duplication	% Proper Pairs	% Aligned	M Aligned	% Duplication	% > Q30	Mb Q30 bases	M Reads After Filtering	GC content	% PF	% Adapter	% Dups	% GC	Average Read Length	Median Read Length	% Failed	M Seqs	% Dups	% GC	Average Read Length	Median Read Length	% Failed	M Seqs
1173_9C_T1_RNA_SLD_01_A23T55JLT4_1	112.5bp	107.0bp	0.01%	0.76%	0.47%	0.00%	0.02%	2.33%	0.72%	0.00%	0.01	588462536	0.01%	0.50%	291466250	0.94%	0.96%	0.05%	0.04%	273590738	137111597	13328178	5020766	424.86	190.00	0.74%	0.61%	0.97	0.000	560664912	0.9393	9999000000	387220904	No	0.00%				50.9%	84.6%	94.2%	277.1M	24.8%	97.2%	63801.6Mb	588.5M	57.8%	98.1%	81.3%
1173_9C_T1_RNA_SLD_01_A23T55JLT4_1_1																																				112.5bp	98.2%	289.1M												84.3%	55%	151bp	151bp	27%	351.4M
1173_9C_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		82.3%	56%	151bp	151bp	27%	351.4M
1173_9C_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		83.3%	57%	111bp	112bp	18%	294.2M	83.3%	57%	111bp	112bp	18%	294.2M
1173_9C_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		82.6%	58%	112bp	112bp	9%	294.2M	82.6%	58%	112bp	112bp	9%	294.2M
1173_BEE_T1_RNA_SLD_01_A23T55JLT4_1	111.5bp	105.0bp	0.01%	1.95%	0.42%	0.00%	0.01%	5.59%	0.68%	0.00%	0.01	565174502	0.01%	0.51%	277994044	0.93%	0.96%	0.05%	0.04%	258970123	129790076	14552614	5815016	396.63	189.00	0.73%	0.60%	0.97	0.000	533082934	0.9188	9999000000	381735119	No	0.00%				51.2%	83.0%	93.3%	263.6M	25.9%	97.4%	60317.9Mb	565.2M	53.5%	98.5%	82.8%
1173_BEE_T1_RNA_SLD_01_A23T55JLT4_1_1																																				111.5bp	97.9%	276.6M												82.9%	52%	151bp	151bp	27%	287.0M
1173_BEE_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		81.0%	53%	151bp	151bp	27%	287.0M
1173_BEE_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		83.0%	53%	109bp	107bp	18%	282.6M	83.0%	53%	109bp	107bp	18%	282.6M
1173_BEE_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		82.2%	53%	110bp	107bp	9%	282.6M	82.2%	53%	110bp	107bp	9%	282.6M
1173_C31_T1_RNA_SLD_01_A23T55JLT4_1	113.3bp	106.0bp	0.03%	1.15%	0.52%	0.00%	0.06%	3.33%	0.77%	0.00%	5.83	205524044	0.01%	0.34%	136263251	0.95%	0.97%	0.04%	0.03%	129496959	64947642	5217327	1934853	203.37	82.00	0.65%	0.46%	0.97	0.000	195666015	0.9188	9999000000	333929478	No	0.00%				34.0%	85.5%	94.3%	97.0M	13.9%	97.3%	22206.3Mb	205.5M	55.2%	98.5%	80.2%
1173_C31_T1_RNA_SLD_01_A23T55JLT4_1_1																																				113.3bp	98.3%	101.0M												74.0%	53%	151bp	151bp	27%	104.3M
1173_C31_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		71.3%	54%	151bp	151bp	27%	104.3M
1173_C31_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		74.0%	54%	111bp	112bp	18%	102.8M	74.0%	54%	111bp	112bp	18%	102.8M
1173_C31_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		72.3%	55%	111bp	112bp	9%	102.8M	72.3%	55%	111bp	112bp	9%	102.8M
1173_C31_T1_RNA_SLD_01_A23T55JLT4_2	113.6bp	107.0bp	0.02%	1.08%	0.52%	0.00%	0.04%	3.17%	0.78%	0.00%	5.76	305780254	0.01%	0.38%	188320050	0.95%	0.97%	0.04%	0.03%	178263193	89366598	7555089	2766660	280.96	119.00	0.69%	0.53%	0.97	0.000	290203706	0.9594	9999000000	380684528	No	0.00%				38.7%	85.1%	93.9%	143.6M	16.5%	97.3%	33197.6Mb	305.8M	56.1%	98.2%	79.7%
1173_C31_T1_RNA_SLD_01_A23T55JLT4_2_1																																				113.6bp	98.3%	150.3M												77.5%	54%	151bp	151bp	27%	155.7M
1173_C31_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		75.5%	55%	151bp	151bp	27%	155.7M
1173_C31_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		77.5%	55%	111bp	112bp	18%	152.9M	77.5%	55%	111bp	112bp	18%	152.9M
1173_C31_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		76.8%	56%	112bp	112bp	9%	152.9M	76.8%	56%	112bp	112bp	9%	152.9M
1173_CH0_T1_RNA_SLD_01_A23T55JLT4_1	108.9bp	104.0bp	0.00%	0.72%	0.36%	0.00%	0.01%	2.25%	0.58%	0.00%	0.03	66507862	0.01%	0.26%	48992977	0.94%	0.96%	0.05%	0.04%	46249438	23159592	2250189	936876	67.41	23.00	0.45%	0.18%	0.97	0.000	63443081	0.9145	9999000000	118783467	No	0.00%				26.5%	85.1%	94.4%	31.4M	12.6%	97.3%	6953.0Mb	66.5M	57.0%	98.6%	86.6%
1173_CH0_T1_RNA_SLD_01_A23T55JLT4_1_1																																				108.9bp	97.9%	32.6M												64.1%	54%	151bp	151bp	27%	33.7M
1173_CH0_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		61.5%	56%	151bp	151bp	27%	33.7M
1173_CH0_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		64.0%	56%	107bp	107bp	18%	33.3M	64.0%	56%	107bp	107bp	18%	33.3M
1173_CH0_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		62.3%	57%	108bp	107bp	9%	33.3M	62.3%	57%	108bp	107bp	9%	33.3M
1173_CH0_T1_RNA_SLD_01_A23T55JLT4_2	109.7bp	104.0bp	0.00%	0.64%	0.35%	0.00%	0.01%	2.04%	0.59%	0.00%	0.01	345689872	0.01%	0.60%	136645184	0.92%	0.95%	0.06%	0.05%	126165247	63123123	7821723	3229116	181.22	69.00	0.63%	0.42%	0.97	0.000	329023882	0.9458	9999000000	120488245	No	0.00%				60.9%	84.4%	94.1%	162.7M	32.0%	97.3%	36413.5Mb	345.7M	58.3%	98.4%	85.2%
1173_CH0_T1_RNA_SLD_01_A23T55JLT4_2_1																																				109.7bp	98.1%	169.6M												83.3%	55%	151bp	151bp	27%	175.7M
1173_CH0_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		81.9%	56%	151bp	151bp	27%	175.7M
1173_CH0_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		83.3%	58%	108bp	107bp	18%	172.8M	83.3%	58%	108bp	107bp	18%	172.8M
1173_CH0_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		83.2%	58%	108bp	107bp	9%	172.8M	83.2%	58%	108bp	107bp	9%	172.8M
1173_CI9_T1_RNA_SLD_01_A23T55JLT4_1	104.1bp	98.0bp	0.01%	0.73%	0.48%	0.00%	0.02%	2.12%	0.71%	0.00%	5.49	488992458	0.01%	0.53%	228075343	0.93%	0.96%	0.05%	0.04%	212337074	106265442	11871312	4796147	302.78	134.00	0.70%	0.55%	0.97	0.000	466361259	0.9344	9999000000	261764196	No	0.00%				53.8%	85.5%	94.7%	231.4M	22.0%	97.3%	49487.8Mb	489.0M	56.1%	98.4%	87.6%
1173_CI9_T1_RNA_SLD_01_A23T55JLT4_1_1																																				104.1bp	98.1%	239.9M												82.1%	53%	151bp	151bp	27%	248.5M
1173_CI9_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		79.6%	55%	151bp	151bp	27%	248.5M
1173_CI9_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		82.1%	55%	104bp	102bp	18%	244.5M	82.1%	55%	104bp	102bp	18%	244.5M
1173_CI9_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		80.8%	56%	104bp	102bp	9%	244.5M	80.8%	56%	104bp	102bp	9%	244.5M
1173_CRL_T1_RNA_SLD_01_A23T55JLT4_1	118.7bp	110.0bp	0.03%	1.20%	0.48%	0.00%	0.05%	3.36%	0.70%	0.00%	0.01	590767176	0.01%	0.45%	325020828	0.94%	0.97%	0.05%	0.03%	306122896	153697144	14885486	5498988	504.79	228.00	0.73%	0.62%	0.97	0.000	566915669	0.9212	9999000000	592940672	No	0.00%				45.3%	87.4%	94.9%	280.2M	23.7%	97.3%	65376.2Mb	590.8M	53.5%	98.5%	76.5%
1173_CRL_T1_RNA_SLD_01_A23T55JLT4_1_1																																				118.7bp	98.0%	289.3M												85.0%	52%	151bp	151bp	27%	490.1M
1173_CRL_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		83.5%	53%	151bp	151bp	27%	490.1M
1173_CRL_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		82.0%	53%	114bp	112bp	18%	295.4M	82.0%	53%	114bp	112bp	18%	295.4M
1173_CRL_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		81.5%	53%	114bp	112bp	9%	295.4M	81.5%	53%	114bp	112bp	9%	295.4M
1173_CRL_T1_RNA_SLD_01_A23T55JLT4_2	124.0bp	116.0bp	0.01%	1.12%	0.48%	0.00%	0.03%	3.22%	0.70%	0.00%	0.01	589293168	0.01%	0.45%	322735502	0.94%	0.97%	0.04%	0.03%	303877596	152587667	14391119	5111974	513.97	239.00	0.73%	0.63%	0.97	0.000	565133812	0.9387	9999000000	501938816	No	0.00%				45.6%	87.3%	94.8%	279.2M	25.1%	97.2%	67362.9Mb	589.3M	54.4%	98.2%	73.5%
1173_CRL_T1_RNA_SLD_01_A23T55JLT4_2_1																																				124.0bp	97.9%	288.5M												84.5%	53%	151bp	151bp	27%	415.7M
1173_CRL_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		82.5%	54%	151bp	151bp	18%	415.7M
1173_CRL_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		82.3%	54%	117bp	117bp	18%	294.6M	82.3%	54%	117bp	117bp	18%	294.6M
1173_CRL_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		81.6%	54%	118bp	122bp	9%	294.6M	81.6%	54%	118bp	122bp	9%	294.6M
1173_CRX_T1_RNA_SLD_01_A23T55JLT4_1	111.8bp	106.0bp	0.02%	1.56%	0.58%	0.00%	0.05%	4.64%	0.89%	0.00%	5.28	22714550	0.01%	0.15%	19231394	0.95%	0.97%	0.04%	0.03%	18333777	9184943	745534	289610	27.62	9.00	0.26%	0.06%	0.97	0.000	21726912	0.9154	9999000000	74447018	No	0.00%				15.4%	85.7%	94.7%	10.8M	6.1%	97.2%	2415.9Mb	22.7M	56.0%	98.2%	83.5%
1173_CRX_T1_RNA_SLD_01_A23T55JLT4_1_1																																				111.8bp	98.1%	11.1M												48.0%	53%	151bp	151bp	18%	11.6M
1173_CRX_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		43.8%	55%	151bp	151bp	18%	11.6M
1173_CRX_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		47.8%	55%	109bp	107bp	9%	11.4M	47.8%	55%	109bp	107bp	9%	11.4M
1173_CRX_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		44.5%	56%	110bp	107bp	0%	11.4M	44.5%	56%	110bp	107bp	0%	11.4M
1173_CRX_T1_RNA_SLD_01_A23T55JLT4_2	119.0bp	113.0bp	0.01%	1.41%	0.56%	0.00%	0.02%	4.32%	0.91%	0.00%	5.19	587317470	0.01%	0.55%	262055027	0.94%	0.96%	0.05%	0.04%	245079191	122722699	12521714	4664345	390.20	168.00	0.70%	0.58%	0.97	0.000	561613438	0.9450	9999000000	262679883	No	0.00%				55.8%	85.8%	94.5%	277.6M	30.7%	97.0%	66237.6Mb	587.3M	57.3%	97.9%	77.7%
1173_CRX_T1_RNA_SLD_01_A23T55JLT4_2_1																																				119.0bp	98.3%	288.6M												83.1%	55%	151bp	151bp	27%	336.5M
1173_CRX_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		81.2%	56%	151bp	151bp	18%	336.5M
1173_CRX_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		82.1%	56%	116bp	117bp	18%	293.7M	82.1%	56%	116bp	117bp	18%	293.7M
1173_CRX_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		81.8%	57%	116bp	117bp	9%	293.7M	81.8%	57%	116bp	117bp	9%	293.7M
1173_D6K_T1_RNA_SLD_01_A23T55JLT4_1	124.8bp	115.0bp	0.01%	1.26%	0.72%	0.00%	0.03%	3.79%	1.03%	0.00%	5.62	590673078	0.01%	0.50%	296561649	0.95%	0.97%	0.04%	0.03%	281323363	141350192	11670717	3933857	492.73	189.00	0.70%	0.59%	0.97	0.000	572788234	0.9568	9999000000	615418316	No	0.00%				50.1%	89.7%	95.8%	282.8M	29.3%	97.3%	67731.8Mb	590.7M	53.8%	98.4%	71.2%
1173_D6K_T1_RNA_SLD_01_A23T55JLT4_1_1																																				124.8bp	98.5%	290.9M												88.8%	52%	151bp	151bp	27%	605.2M
1173_D6K_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		87.1%	53%	151bp	151bp	18%	605.2M
1173_D6K_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		85.7%	53%	118bp	122bp	18%	295.3M	85.7%	53%	118bp	122bp	18%	295.3M
1173_D6K_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		85.0%	54%	118bp	122bp	9%	295.3M	85.0%	54%	118bp	122bp	9%	295.3M
1173_D6K_T1_RNA_SLD_01_A23T55JLT4_2	131.5bp	122.0bp	0.01%	1.17%	0.71%	0.00%	0.02%	3.60%	1.04%	0.00%	5.67	589312870	0.01%	0.48%	308919261	0.95%	0.98%	0.04%	0.02%	293187411	147390378	11825708	3763359	534.66	214.00	0.71%	0.60%	0.97	0.000	571718856	0.9746	9990000000	664767249	No	0.00%				47.9%	90.2%	95.8%	282.1M	28.4%	97.2%	69997.1Mb	589.3M	54.3%	98.2%	66.6%
1173_D6K_T1_RNA_SLD_01_A23T55JLT4_2_1																																				131.5bp	98.5%	290.4M												89.6%	53%	151bp	151bp	27%	779.6M
1173_D6K_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		87.9%	54%	151bp	151bp	18%	779.6M
1173_D6K_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		85.3%	53%	122bp	127bp	18%	294.7M	85.3%	53%	122bp	127bp	18%	294.7M
1173_D6K_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		84.9%	54%	122bp	127bp	9%	294.7M	84.9%	54%	122bp	127bp	9%	294.7M
1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1	108.0bp	101.0bp	0.03%	1.13%	0.47%	0.00%	0.07%	3.29%	0.73%	0.00%	5.47	589671226	0.01%	0.49%	301697187	0.94%	0.96%	0.05%	0.04%	283944635	142302232	13765240	5309844	430.24	172.00	0.72%	0.59%	0.97	0.000	545121328	0.9153	9999000000	499203542	No	0.00%				49.2%	78.1%	91.6%	270.2M	20.6%	97.2%	61174.1Mb	589.7M	57.8%	98.3%	83.4%
1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1_1																																				108.0bp	98.2%	289.5M												85.5%	55%	151bp	151bp	27%	605.7M
1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		83.7%	56%	151bp	151bp	27%	605.7M
1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		81.1%	57%	107bp	102bp	18%	294.8M	81.1%	57%	107bp	102bp	18%	294.8M
1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		80.4%	58%	107bp	107bp	9%	294.8M	80.4%	58%	107bp	107bp	9%	294.8M
1173_DSO_T1_RNA_SLD_01_A23T55JLT4_2	119.2bp	112.0bp	0.02%	1.00%	0.46%	0.00%	0.04%	3.00%	0.72%	0.00%	5.49	412555804	0.01%	0.43%	233472998	0.94%	0.97%	0.04%	0.03%	220264243	110480300	10035469	3668753	361.12	136.00	0.69%	0.55%	0.97	0.000	381631336	0.9559	9999000000	397191951	No	0.00%				43.7%	78.4%	91.4%	188.6M	23.1%	97.2%	46421.0Mb	412.6M	58.2%	98.0%	76.3%
1173_DSO_T1_RNA_SLD_01_A23T55JLT4_2_1																																				119.2bp	98.2%	202.5M												79.0%	56%	151bp	151bp	27%	210.4M
1173_DSO_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		77.4%	56%	151bp	151bp	27%	210.4M
1173_DSO_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		79.0%	57%	116bp	117bp	18%	206.3M	79.0%	57%	116bp	117bp	18%	206.3M
1173_DSO_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		78.8%	58%	116bp	117bp	9%	206.3M	78.8%	58%	116bp	117bp	9%	206.3M
1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_1	104.8bp	100.0bp	0.06%	1.55%	0.51%	0.00%	0.12%	4.36%	0.78%	0.00%	4.09	590320828	0.01%	0.68%	191583757	0.93%	0.96%	0.05%	0.04%	177566253	88857919	10129925	4011006	248.26	102.00	0.67%	0.50%	0.97	0.000	549884621	0.8941	9999000000	164666820	No	0.00%				68.0%	79.7%	92.4%	272.8M	35.3%	97.3%	60567.7Mb	590.3M	56.5%	98.4%	87.4%
1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_1_1																																				104.8bp	98.3%	290.1M												87.4%	53%	151bp	151bp	27%	383.9M
1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		85.6%	55%	151bp	151bp	27%	383.9M
1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		85.7%	56%	105bp	102bp	18%	295.2M	85.7%	56%	105bp	102bp	18%	295.2M
1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		85.0%	56%	106bp	102bp	9%	295.2M	85.0%	56%	106bp	102bp	9%	295.2M
1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2	109.6bp	105.0bp	0.03%	1.38%	0.50%	0.00%	0.06%	3.95%	0.79%	0.00%	3.92	573354668	0.01%	0.76%	136583482	0.91%	0.95%	0.06%	0.05%	123629951	61821915	8266698	3203318	175.86	63.00	0.61%	0.41%	0.97	0.000	531254564	0.9447	9999000000	105863408	No	0.00%				76.8%	77.9%	91.7%	262.8M	43.3%	97.1%	61064.5Mb	573.4M	58.3%	97.9%	83.9%
1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2_1																																				109.6bp	98.1%	281.1M												87.9%	55%	151bp	151bp	27%	292.9M
1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		86.0%	56%	151bp	151bp	27%	292.9M
1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		88.0%	58%	109bp	107bp	18%	286.7M	88.0%	58%	109bp	107bp	18%	286.7M
1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		87.7%	58%	110bp	107bp	9%	286.7M	87.7%	58%	110bp	107bp	9%	286.7M
1173_FM_T1_RNA_SLD_01_A23T55JLT4_1	101.2bp	97.0bp	0.03%	0.86%	0.57%	0.00%	0.06%	2.50%	0.87%	0.00%	0.03	587458318	0.01%	0.65%	205352357	0.92%	0.95%	0.05%	0.05%	189409690	94704545	11245037	4485567	256.54	85.00	0.65%	0.47%	0.97	0.000	558612273	0.9255	9990000000	211570891	Yes	0.00%				65.6%	83.5%	94.2%	276.7M	27.2%	96.6%	58291.7Mb	587.5M	60.4%	97.9%	89.1%
1173_FM_T1_RNA_SLD_01_A23T55JLT4_1_1																																				101.2bp	98.0%	287.7M												86.9%	56%	151bp	151bp	27%	495.7M
1173_FM_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		84.6%	57%	151bp	151bp	27%	495.7M
1173_FM_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		83.3%	60%	102bp	102bp	18%	293.7M	83.3%	60%	102bp	102bp	18%	293.7M
1173_FM_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		82.5%	60%	103bp	102bp	9%	293.7M	82.5%	60%	103bp	102bp	9%	293.7M
1173_FM_T1_RNA_SLD_01_A23T55JLT4_2	107.8bp	102.0bp	0.01%	0.72%	0.54%	0.00%	0.03%	2.19%	0.87%	0.00%	0.01	585843570	0.01%	0.70%	177814621	0.91%	0.95%	0.06%	0.05%	162227313	81133098	10044318	3932028	228.10	65.00	0.61%	0.43%	0.97	0.000	556626824	0.9488	9999000000	150744756	No	0.00%				70.3%	83.0%	93.8%	274.6M	36.4%	97.0%	61512.5Mb	585.8M	61.7%	97.6%	85.3%
1173_FM_T1_RNA_SLD_01_A23T55JLT4_2_1																																				107.8bp	97.9%	286.9M												86.6%	58%	151bp	151bp	27%	371.3M
1173_FM_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		84.6%	59%	151bp	151bp	27%	371.3M
1173_FM_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		85.0%	61%	108bp	107bp	18%	292.9M	85.0%	61%	108bp	107bp	18%	292.9M
1173_FM_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		84.8%	61%	108bp	107bp	9%	292.9M	84.8%	61%	108bp	107bp	9%	292.9M
1173_FWA_T1_RNA_SLD_01_A23T55JLT4_1	125.1bp	117.0bp	0.02%	1.01%	0.57%	0.00%	0.03%	3.00%	0.88%	0.00%	4.73	588279586	0.01%	0.51%	291141523	0.94%	0.97%	0.05%	0.03%	273233984	137076639	13293038	4799346	462.01	216.00	0.73%	0.61%	0.97	0.000	558210605	0.9189	9999000000	325342077	Yes	0.00%				50.9%	84.6%	94.0%	276.4M	27.6%	97.2%	67928.3Mb	588.3M	56.8%	98.0%	72.6%
1173_FWA_T1_RNA_SLD_01_A23T55JLT4_1_1																																				125.1bp	98.2%	289.0M												82.2%	54%	151bp	151bp	18%	394.8M
1173_FWA_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		80.7%	56%	151bp	151bp	18%	394.8M
1173_FWA_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		79.9%	56%	119bp	122bp	9%	294.1M	79.9%	56%	119bp	122bp	9%	294.1M
1173_FWA_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		79.9%	57%	119bp	122bp	9%	294.1M	79.9%	57%	119bp	122bp	9%	294.1M
1173_FWA_T1_RNA_SLD_01_A23T55JLT4_2	104.9bp	31.0bp	0.00%	0.00%	0.00%	0.00%	0.00%	0.00%	0.00%	0.00%	0.00	19646	0.51%	0.36%	12531	0.01%	0.07%	0.19%	0.93%	169	84	2396	1052	0.00	0.00	0.00%	0.00%	0.93	0.000	1801	0.3553	9999000000	11470	Skipped	N/A%				73.5%	1.9%	7.2%	0.0M	32.6%	85.5%	1.4Mb	0.0M	71.6%	5.9%	50.9%
1173_FWA_T1_RNA_SLD_01_A23T55JLT4_2_1																																				104.9bp	8.2%	0.0M												96.2%	74%	151bp	151bp	45%	0.2M
1173_FWA_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		93.0%	94%	151bp	151bp	55%	0.2M
1173_FWA_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		54.5%	67%	71bp	37bp	55%	0.0M	54.5%	67%	71bp	37bp	55%	0.0M
1173_FWA_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		57.9%	74%	101bp	122bp	55%	0.0M	57.9%	74%	101bp	122bp	55%	0.0M
1173_G2E_T1_RNA_SLD_01_A23T55JLT4_1	103.2bp	99.0bp	0.01%	0.87%	0.36%	0.00%	0.01%	2.53%	0.56%	0.00%	0.03	587687582	0.01%	0.66%	197601879	0.91%	0.95%	0.06%	0.05%	179872922	89897256	12232193	5054075	245.91	92.00	0.66%	0.49%	0.97	0.000	552102396	0.8508	9999000000	186985242	No	0.00%				67.0%	80.8%	93.0%	273.4M	29.5%	97.1%	59160.5Mb	587.7M	59.2%	97.9%	88.6%
1173_G2E_T1_RNA_SLD_01_A23T55JLT4_1_1																																				103.2bp	97.9%	287.6M												85.4%	56%	151bp	151bp	27%	400.4M
1173_G2E_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		83.4%	57%	151bp	151bp	27%	400.4M
1173_G2E_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		83.2%	58%	103bp	102bp	18%	293.8M	83.2%	58%	103bp	102bp	18%	293.8M
1173_G2E_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		82.7%	59%	104bp	102bp	9%	293.8M	82.7%	59%	104bp	102bp	9%	293.8M
1173_G2E_T1_RNA_SLD_01_A23T55JLT4_2	107.6bp	102.0bp	0.01%	0.83%	0.35%	0.00%	0.01%	2.46%	0.55%	0.00%	0.03	563933476	0.01%	0.74%	146822289	0.90%	0.95%	0.06%	0.05%	131910641	65885943	9172542	3691554	185.15	68.00	0.63%	0.43%	0.97	0.000	528214804	0.9006	9999000000	116101252	No	0.00%				74.7%	80.3%	92.7%	261.4M	44.5%	97.2%	58708.7Mb	563.9M	58.8%	97.8%	86.0%
1173_G2E_T1_RNA_SLD_01_A23T55JLT4_2_1																																				107.6bp	98.0%	276.5M												86.5%	55%	151bp	151bp	27%	288.4M
1173_G2E_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		84.5%	57%	151bp	151bp	27%	288.4M
1173_G2E_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		86.5%	58%	107bp	107bp	18%	282.0M	86.5%	58%	107bp	107bp	18%	282.0M
1173_G2E_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		86.2%	59%	107bp	107bp	9%	282.0M	86.2%	59%	107bp	107bp	9%	282.0M
1173_GFO_T1_RNA_SLD_01_A23T55JLT4_1	116.6bp	113.0bp	0.01%	0.56%	0.29%	0.00%	0.01%	1.77%	0.47%	0.00%	0.02	580907912	0.01%	0.67%	190438066	0.90%	0.95%	0.06%	0.05%	171478951	85820999	11521926	4612964	258.79	117.00	0.71%	0.53%	0.97	0.000	545508186	0.9046	9999000000	168810340	No	0.00%				68.1%	82.4%	92.8%	269.7M	38.8%	97.2%	64465.2Mb	580.9M	57.9%	98.0%	80.8%
1173_GFO_T1_RNA_SLD_01_A23T55JLT4_1_1																																				116.6bp	98.1%	285.0M												85.0%	55%	151bp	151bp	27%	296.5M
1173_GFO_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		82.7%	57%	151bp	151bp	27%	296.5M
1173_GFO_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		85.1%	57%	114bp	117bp	18%	290.5M	85.1%	57%	114bp	117bp	18%	290.5M
1173_GFO_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		84.2%	58%	114bp	117bp	9%	290.5M	84.2%	58%	114bp	117bp	9%	290.5M
1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2	117.5bp	112.0bp	0.00%	0.53%	0.28%	0.00%	0.01%	1.68%	0.46%	0.00%	0.03	483494574	0.01%	0.71%	142051207	0.89%	0.95%	0.06%	0.05%	126236822	63146102	8866096	3530541	189.17	81.00	0.67%	0.45%	0.97	0.000	452778298	0.9374	9999000000	122977893	No	0.00%				71.6%	82.0%	92.5%	223.7M	44.1%	97.2%	53808.8Mb	483.5M	58.2%	97.8%	80.3%
1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2_1																																				117.5bp	98.1%	237.2M												85.9%	56%	151bp	151bp	27%	247.1M
1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		83.7%	57%	151bp	151bp	27%	247.1M
1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		85.9%	57%	114bp	117bp	18%	241.7M	85.9%	57%	114bp	117bp	18%	241.7M
1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		85.4%	58%	115bp	117bp	9%	241.7M	85.4%	58%	115bp	117bp	9%	241.7M
1173_GXL_T1_RNA_SLD_01_A23T55JLT4_1	106.8bp	101.0bp	0.01%	0.79%	0.41%	0.00%	0.02%	2.35%	0.60%	0.00%	5.75	589747158	0.01%	0.63%	215615027	0.92%	0.95%	0.06%	0.05%	199407309	99834590	12100359	4857890	287.30	128.00	0.72%	0.55%	0.97	0.000	564851800	0.8687	9999000000	190834154	No	0.00%				63.9%	86.4%	94.9%	279.8M	29.9%	97.1%	60985.9Mb	589.7M	57.6%	98.3%	85.3%
1173_GXL_T1_RNA_SLD_01_A23T55JLT4_1_1																																				106.8bp	98.2%	289.7M												86.2%	55%	151bp	151bp	27%	413.8M
1173_GXL_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		84.2%	56%	151bp	151bp	27%	413.8M
1173_GXL_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		83.7%	57%	106bp	102bp	18%	294.9M	83.7%	57%	106bp	102bp	18%	294.9M
1173_GXL_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		83.0%	57%	107bp	107bp	9%	294.9M	83.0%	57%	107bp	107bp	9%	294.9M
1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2	114.2bp	108.0bp	0.01%	0.74%	0.41%	0.00%	0.01%	2.24%	0.63%	0.00%	5.79	565513486	0.01%	0.71%	163378841	0.91%	0.95%	0.06%	0.05%	148869541	74500724	10073759	4003778	215.74	92.00	0.68%	0.48%	0.97	0.000	540944677	0.9147	9999000000	136330590	No	0.00%				71.7%	85.7%	94.6%	267.4M	40.2%	97.2%	61298.7Mb	565.5M	57.9%	98.0%	81.7%
1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2_1																																				114.2bp	98.2%	277.6M												86.5%	55%	151bp	151bp	27%	288.5M
1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		84.3%	56%	151bp	151bp	27%	288.5M
1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		86.5%	57%	111bp	112bp	18%	282.8M	86.5%	57%	111bp	112bp	18%	282.8M
1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		85.9%	58%	112bp	112bp	9%	282.8M	85.9%	58%	112bp	112bp	9%	282.8M
1173_GZ1_T1_RNA_SLD_01_A23T55JLT4_1	114.1bp	108.0bp	0.02%	0.74%	0.35%	0.00%	0.04%	2.30%	0.55%	0.00%	5.68	30843242	0.01%	0.18%	25190386	0.95%	0.97%	0.04%	0.03%	23959890	12015220	981257	377820	36.87	15.00	0.34%	0.08%	0.97	0.000	29383500	0.8954	9999000000	89192228	No	0.00%				18.5%	85.7%	94.5%	14.6M	7.8%	97.3%	3319.5Mb	30.8M	55.0%	98.5%	81.6%
1173_GZ1_T1_RNA_SLD_01_A23T55JLT4_1_1																																				114.1bp	98.4%	15.2M												54.2%	53%	151bp	151bp	27%	15.7M
1173_GZ1_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		49.4%	54%	151bp	151bp	18%	15.7M
1173_GZ1_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		54.1%	54%	110bp	107bp	18%	15.4M	54.1%	54%	110bp	107bp	18%	15.4M
1173_GZ1_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		50.0%	55%	111bp	112bp	0%	15.4M	50.0%	55%	111bp	112bp	0%	15.4M
1173_GZ1_T1_RNA_SLD_01_A23T55JLT4_2	116.9bp	110.0bp	0.01%	0.68%	0.34%	0.00%	0.02%	2.17%	0.54%	0.00%	5.60	560658426	0.01%	0.57%	238437358	0.93%	0.96%	0.05%	0.04%	221433753	111059402	11837420	4482171	349.68	187.00	0.77%	0.63%	0.97	0.000	533412435	0.9489	9999000000	230091914	Yes	0.05%				58.0%	85.3%	94.2%	264.0M	32.0%	97.2%	62271.7Mb	560.7M	56.0%	98.2%	78.7%
1173_GZ1_T1_RNA_SLD_01_A23T55JLT4_2_1																																				116.9bp	98.4%	275.8M												84.7%	53%	151bp	151bp	27%	285.4M
1173_GZ1_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		82.6%	55%	151bp	151bp	27%	285.4M
1173_GZ1_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		84.7%	55%	114bp	112bp	18%	280.3M	84.7%	55%	114bp	112bp	18%	280.3M
1173_GZ1_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		83.9%	56%	114bp	112bp	9%	280.3M	83.9%	56%	114bp	112bp	9%	280.3M
1173_H2E_T1_RNA_SLD_01_A23T55JLT4_1	116.5bp	107.0bp	0.01%	0.85%	0.39%	0.00%	0.03%	2.48%	0.56%	0.00%	0.01	588709214	0.01%	0.45%	321842484	0.94%	0.96%	0.05%	0.04%	301866264	151543384	15413405	5672996	491.08	231.00	0.77%	0.64%	0.97	0.000	564132820	0.9247	9999000000	554132957	No	0.00%				45.7%	87.0%	95.0%	279.5M	22.4%	97.1%	64696.2Mb	588.7M	56.9%	98.1%	76.5%
1173_H2E_T1_RNA_SLD_01_A23T55JLT4_1_1																																				116.5bp	98.2%	288.9M												87.8%	54%	151bp	151bp	27%	724.1M
1173_H2E_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		85.5%	56%	151bp	151bp	27%	724.1M
1173_H2E_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		82.8%	56%	113bp	112bp	18%	294.4M	82.8%	56%	113bp	112bp	18%	294.4M
1173_H2E_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		81.6%	57%	113bp	112bp	9%	294.4M	81.6%	57%	113bp	112bp	9%	294.4M
1173_H2E_T1_RNA_SLD_01_A23T55JLT4_2	129.1bp	121.0bp	0.01%	0.76%	0.36%	0.00%	0.01%	2.28%	0.54%	0.00%	0.01	588121648	0.01%	0.46%	319097081	0.94%	0.97%	0.05%	0.03%	299202429	150381917	14970206	5221471	522.35	250.00	0.77%	0.66%	0.97	0.000	564595073	0.9515	9990000000	481077006	No	0.00%				46.1%	87.7%	95.0%	279.3M	26.4%	97.0%	69270.8Mb	588.1M	56.9%	98.0%	68.8%
1173_H2E_T1_RNA_SLD_01_A23T55JLT4_2_1																																				129.1bp	98.2%	288.7M												85.5%	55%	151bp	151bp	27%	419.0M
1173_H2E_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		83.2%	56%	151bp	151bp	18%	419.0M
1173_H2E_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		83.3%	56%	121bp	127bp	18%	294.1M	83.3%	56%	121bp	127bp	18%	294.1M
1173_H2E_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		82.4%	57%	122bp	127bp	9%	294.1M	82.4%	57%	122bp	127bp	9%	294.1M
1173_HOB_T1_RNA_SLD_01_A23T55JLT4_1	109.1bp	103.0bp	0.02%	1.36%	0.67%	0.00%	0.04%	3.75%	0.92%	0.00%	0.01	589610250	0.01%	0.50%	296575557	0.94%	0.97%	0.05%	0.03%	279060730	139918407	13359949	4993456	428.75	162.00	0.71%	0.58%	0.97	0.000	558477069	0.9161	9999000000	452975029	Yes	0.00%				50.1%	83.3%	93.9%	276.8M	23.4%	97.2%	62524.6Mb	589.6M	58.0%	98.3%	82.3%
1173_HOB_T1_RNA_SLD_01_A23T55JLT4_1_1																																				109.1bp	98.0%	289.0M												87.5%	55%	151bp	151bp	27%	663.3M
1173_HOB_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		85.6%	56%	151bp	151bp	27%	663.3M
1173_HOB_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		83.0%	57%	109bp	107bp	18%	294.8M	83.0%	57%	109bp	107bp	18%	294.8M
1173_HOB_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		82.2%	58%	109bp	107bp	9%	294.8M	82.2%	58%	109bp	107bp	9%	294.8M
1173_HOB_T1_RNA_SLD_01_A23T55JLT4_2	113.6bp	106.0bp	0.01%	1.25%	0.66%	0.00%	0.02%	3.52%	0.94%	0.00%	0.01	475785136	0.01%	0.48%	248292868	0.94%	0.97%	0.04%	0.03%	233721536	117174205	10856013	3904241	366.58	126.00	0.68%	0.54%	0.97	0.000	449599114	0.9475	9999000000	348178125	Yes	0.00%				48.2%	82.4%	93.5%	222.3M	22.1%	97.1%	51486.5Mb	475.8M	59.0%	98.0%	80.3%
1173_HOB_T1_RNA_SLD_01_A23T55JLT4_2_1																																				113.6bp	98.0%	233.2M												82.2%	55%	151bp	151bp	27%	242.8M
1173_HOB_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		80.4%	58%	151bp	151bp	27%	242.8M
1173_HOB_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		82.3%	58%	111bp	112bp	18%	237.9M	82.3%	58%	111bp	112bp	18%	237.9M
1173_HOB_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		82.0%	59%	112bp	112bp	9%	237.9M	82.0%	59%	112bp	112bp	9%	237.9M
1173_HRO_T1_RNA_SLD_01_A23T55JLT4_1	105.3bp	100.0bp	0.01%	0.66%	0.30%	0.00%	0.03%	1.87%	0.44%	0.00%	5.58	589130000	0.01%	0.64%	209374394	0.93%	0.95%	0.05%	0.05%	193850905	96995485	11364894	4588360	277.93	136.00	0.73%	0.56%	0.97	0.000	555930132	0.9232	9999000000	195907367	Yes	0.00%				64.9%	83.4%	93.6%	275.7M	29.1%	97.2%	60342.1Mb	589.1M	57.1%	98.2%	86.8%
1173_HRO_T1_RNA_SLD_01_A23T55JLT4_1_1																																				105.3bp	98.0%	288.7M												85.6%	54%	151bp	151bp	27%	402.3M
1173_HRO_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		83.5%	56%	151bp	151bp	27%	402.3M
1173_HRO_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		83.3%	56%	105bp	102bp	18%	294.6M	83.3%	56%	105bp	102bp	18%	294.6M
1173_HRO_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		82.5%	57%	106bp	102bp	9%	294.6M	82.5%	57%	106bp	102bp	9%	294.6M
1173_IT6_T1_RNA_SLD_01_A23T55JLT4_1	101.2bp	95.0bp	0.04%	0.76%	0.45%	0.00%	0.09%	2.11%	0.62%	0.00%	0.01	522531886	0.01%	0.62%	200857801	0.93%	0.95%	0.06%	0.05%	186038418	93093479	11367840	4717404	257.03	98.00	0.65%	0.50%	0.97	0.000	497516232	0.9267	9999000000	188526454	No	0.00%				62.0%	82.7%	94.7%	247.5M	25.9%	97.4%	51518.2Mb	522.5M	53.5%	98.6%	89.2%
1173_IT6_T1_RNA_SLD_01_A23T55JLT4_1_1																																				101.2bp	97.8%	255.5M												84.6%	52%	151bp	151bp	27%	264.9M
1173_IT6_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		83.1%	53%	151bp	151bp	27%	264.9M
1173_IT6_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		84.7%	53%	101bp	97bp	18%	261.3M	84.7%	53%	101bp	97bp	18%	261.3M
1173_IT6_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		84.1%	53%	101bp	97bp	9%	261.3M	84.1%	53%	101bp	97bp	9%	261.3M
1173_IT6_T1_RNA_SLD_01_A23T55JLT4_2	106.2bp	101.0bp	0.02%	0.73%	0.48%	0.00%	0.04%	2.09%	0.66%	0.00%	0.01	300330088	0.01%	0.48%	156292265	0.94%	0.96%	0.05%	0.04%	146856258	73531222	7193500	2798148	212.95	80.00	0.63%	0.46%	0.97	0.000	287223857	0.9549	9999000000	191441011	No	0.00%				48.3%	85.1%	94.8%	142.3M	20.5%	97.3%	30908.0Mb	300.3M	55.6%	98.2%	86.3%
1173_IT6_T1_RNA_SLD_01_A23T55JLT4_2_1																																				106.2bp	98.3%	147.6M												80.2%	52%	151bp	151bp	27%	152.9M
1173_IT6_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		78.3%	55%	151bp	151bp	27%	152.9M
1173_IT6_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		80.2%	55%	106bp	102bp	18%	150.2M	80.2%	55%	106bp	102bp	18%	150.2M
1173_IT6_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		79.6%	55%	106bp	102bp	9%	150.2M	79.6%	55%	106bp	102bp	9%	150.2M
1173_J3R_T1_RNA_SLD_01_A23T55JLT4_1	91.9bp	88.0bp	0.01%	1.05%	0.39%	0.00%	0.02%	3.18%	0.60%	0.00%	0.17	472800642	0.01%	0.65%	165367285	0.91%	0.94%	0.07%	0.06%	150706779	75255626	11325135	4927243	185.37	83.00	0.66%	0.46%	0.97	0.000	444271201	0.8835	9999000000	148337795	No	0.00%				65.5%	80.1%	93.2%	220.4M	22.2%	97.3%	42878.4Mb	472.8M	55.5%	98.4%	94.2%
1173_J3R_T1_RNA_SLD_01_A23T55JLT4_1_1																																				91.9bp	97.9%	231.4M												82.0%	52%	151bp	151bp	27%	240.2M
1173_J3R_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		80.5%	55%	151bp	151bp	27%	240.2M
1173_J3R_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		82.1%	55%	93bp	92bp	18%	236.4M	82.1%	55%	93bp	92bp	18%	236.4M
1173_J3R_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		81.6%	55%	93bp	92bp	9%	236.4M	81.6%	55%	93bp	92bp	9%	236.4M
1173_J3R_T1_RNA_SLD_01_A23T55JLT4_2	99.4bp	95.0bp	0.01%	0.94%	0.37%	0.00%	0.01%	2.98%	0.58%	0.00%	0.15	349997100	0.01%	0.66%	118240962	0.91%	0.94%	0.06%	0.06%	107937463	53872653	7469305	3141604	139.61	62.00	0.63%	0.39%	0.97	0.000	328404633	0.9343	9999000000	95818199	No	0.00%				66.8%	79.3%	92.7%	162.2M	28.2%	97.2%	34218.0Mb	350.0M	55.3%	97.9%	91.4%
1173_J3R_T1_RNA_SLD_01_A23T55JLT4_2_1																																				99.4bp	98.2%	171.9M												82.8%	52%	151bp	151bp	27%	178.7M
1173_J3R_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		80.6%	55%	151bp	151bp	27%	178.7M
1173_J3R_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		82.8%	55%	100bp	97bp	18%	175.0M	82.8%	55%	100bp	97bp	18%	175.0M
1173_J3R_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		82.3%	55%	101bp	97bp	9%	175.0M	82.3%	55%	101bp	97bp	9%	175.0M
1173_JIG_T1_RNA_SLD_01_A23T55JLT4_1	90.2bp	83.0bp	0.07%	0.80%	0.29%	0.00%	0.14%	2.16%	0.45%	0.00%	5.33	55214792	0.03%	0.52%	26712223	0.86%	0.91%	0.09%	0.09%	22878895	11443081	2334437	1064500	29.22	7.00	0.24%	0.07%	0.97	0.000	35173070	0.8779	9999000000	48283151	No	0.00%				53.1%	18.7%	62.9%	17.4M	18.4%	97.0%	4292.3Mb	55.2M	55.4%	98.3%	93.2%
1173_JIG_T1_RNA_SLD_01_A23T55JLT4_1_1																																				90.2bp	69.1%	19.1M												69.4%	55%	151bp	151bp	27%	28.1M
1173_JIG_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		66.6%	56%	151bp	151bp	27%	28.1M
1173_JIG_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		69.7%	55%	80bp	77bp	27%	27.6M	69.7%	55%	80bp	77bp	27%	27.6M
1173_JIG_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		67.8%	55%	81bp	77bp	27%	27.6M	67.8%	55%	81bp	77bp	27%	27.6M
1173_JLC_T1_RNA_SLD_01_A23T55JLT4_1	124.0bp	115.0bp	0.01%	1.07%	0.61%	0.00%	0.02%	3.16%	0.87%	0.00%	0.01	589784770	0.01%	0.43%	334228104	0.94%	0.97%	0.04%	0.03%	314827433	158175857	14924736	5324508	538.98	251.00	0.75%	0.64%	0.97	0.000	565189064	0.9531	9999000000	677069762	No	0.00%				43.7%	86.8%	94.8%	279.5M	24.8%	97.3%	67337.6Mb	589.8M	56.5%	98.3%	71.8%
1173_JLC_T1_RNA_SLD_01_A23T55JLT4_1_1																																				124.0bp	98.0%	289.0M												88.4%	54%	151bp	151bp	27%	745.2M
1173_JLC_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		86.7%	55%	151bp	151bp	18%	745.2M
1173_JLC_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		83.6%	56%	117bp	117bp	18%	294.9M	83.6%	56%	117bp	117bp	18%	294.9M
1173_JLC_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		83.0%	56%	117bp	122bp	9%	294.9M	83.0%	56%	117bp	122bp	9%	294.9M
1173_JLC_T1_RNA_SLD_01_A23T55JLT4_2	131.0bp	122.0bp	0.01%	1.01%	0.58%	0.00%	0.01%	3.03%	0.84%	0.00%	0.01	588693102	0.01%	0.42%	341173819	0.94%	0.97%	0.04%	0.03%	321335918	161536906	14942949	5083384	574.94	269.00	0.76%	0.65%	0.97	0.000	564280394	0.9657	9999000000	645494598	No	0.00%				42.4%	86.8%	94.6%	278.5M	25.0%	97.2%	69860.8Mb	588.7M	56.8%	98.1%	67.0%
1173_JLC_T1_RNA_SLD_01_A23T55JLT4_2_1																																				131.0bp	97.9%	288.3M												87.1%	55%	151bp	151bp	27%	572.8M
1173_JLC_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		85.3%	56%	151bp	151bp	18%	572.8M
1173_JLC_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		83.5%	56%	122bp	127bp	18%	294.3M	83.5%	56%	122bp	127bp	18%	294.3M
1173_JLC_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		83.1%	57%	122bp	127bp	9%	294.3M	83.1%	57%	122bp	127bp	9%	294.3M
1173_JXL_T1_RNA_SLD_01_A23T55JLT4_1	121.5bp	111.0bp	0.09%	1.51%	0.46%	0.00%	0.16%	4.15%	0.66%	0.00%	6.20	531893406	0.01%	0.47%	280201962	0.94%	0.97%	0.04%	0.03%	264126449	132542310	12514599	4504027	448.60	143.00	0.65%	0.54%	0.97	0.000	507930998	0.9337	9999000000	458852873	No	0.00%				47.6%	81.8%	94.5%	251.2M	26.9%	97.3%	59532.1Mb	531.9M	51.9%	98.4%	73.6%
1173_JXL_T1_RNA_SLD_01_A23T55JLT4_1_1																																				121.5bp	98.0%	260.6M												84.6%	51%	151bp	151bp	27%	270.2M
1173_JXL_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		82.7%	52%	151bp	151bp	27%	270.2M
1173_JXL_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		84.6%	51%	115bp	117bp	18%	265.9M	84.6%	51%	115bp	117bp	18%	265.9M
1173_JXL_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		83.9%	52%	115bp	117bp	18%	265.9M	83.9%	52%	115bp	117bp	18%	265.9M
1173_JXL_T1_RNA_SLD_01_A23T55JLT4_2	128.0bp	118.0bp	0.06%	1.43%	0.46%	0.00%	0.10%	4.01%	0.67%	0.00%	6.11	589842814	0.01%	0.46%	316905798	0.95%	0.97%	0.04%	0.03%	299794404	150580716	13302872	4540736	528.10	180.00	0.67%	0.57%	0.97	0.000	568058622	0.9588	9999000000	540841962	No	0.00%				46.6%	87.5%	95.2%	280.9M	25.8%	97.3%	68387.4Mb	589.8M	52.9%	98.3%	70.4%
1173_JXL_T1_RNA_SLD_01_A23T55JLT4_2_1																																				128.0bp	98.5%	290.4M												86.9%	52%	151bp	151bp	27%	401.2M
1173_JXL_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		84.6%	53%	151bp	151bp	18%	401.2M
1173_JXL_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		85.4%	52%	119bp	122bp	18%	294.9M	85.4%	52%	119bp	122bp	18%	294.9M
1173_JXL_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		84.2%	53%	119bp	122bp	9%	294.9M	84.2%	53%	119bp	122bp	9%	294.9M
1173_K3O_T1_RNA_SLD_01_A23T55JLT4_1	95.4bp	91.0bp	0.01%	0.90%	0.30%	0.00%	0.02%	2.59%	0.46%	0.00%	5.87	28389066	0.01%	0.21%	22523975	0.94%	0.96%	0.05%	0.04%	21226132	10615412	1066338	459326	26.92	10.00	0.25%	0.05%	0.98	0.000	26957785	0.8610	9999000000	62762443	No	0.00%				20.8%	84.1%	94.1%	13.4M	7.8%	97.4%	2634.0Mb	28.4M	56.0%	98.3%	93.5%
1173_K3O_T1_RNA_SLD_01_A23T55JLT4_1_1																																				95.4bp	97.5%	13.8M												44.2%	53%	151bp	151bp	18%	14.4M
1173_K3O_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		40.6%	55%	151bp	151bp	18%	14.4M
1173_K3O_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		44.0%	55%	95bp	92bp	9%	14.2M	44.0%	55%	95bp	92bp	9%	14.2M
1173_K3O_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		41.1%	56%	95bp	92bp	0%	14.2M	41.1%	56%	95bp	92bp	0%	14.2M
1173_K3O_T1_RNA_SLD_01_A23T55JLT4_2	101.0bp	97.0bp	0.01%	0.82%	0.30%	0.00%	0.01%	2.43%	0.46%	0.00%	5.91	294192922	0.01%	0.62%	110702001	0.91%	0.94%	0.07%	0.06%	100600299	50255735	7230402	3108347	129.87	59.00	0.63%	0.37%	0.97	0.000	278837614	0.8944	9999000000	95584731	No	0.00%				63.0%	83.0%	93.6%	137.7M	25.7%	97.2%	28910.8Mb	294.2M	56.5%	98.1%	91.1%
1173_K3O_T1_RNA_SLD_01_A23T55JLT4_2_1																																				101.0bp	97.5%	143.5M												76.5%	53%	151bp	151bp	27%	149.9M
1173_K3O_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		74.6%	56%	151bp	151bp	27%	149.9M
1173_K3O_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		76.5%	56%	101bp	97bp	18%	147.1M	76.5%	56%	101bp	97bp	18%	147.1M
1173_K3O_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		75.8%	56%	101bp	97bp	9%	147.1M	75.8%	56%	101bp	97bp	9%	147.1M
1173_K7E_T1_RNA_SLD_01_A23T55JLT4_1	101.7bp	96.0bp	0.01%	2.48%	0.59%	0.00%	0.02%	6.54%	0.87%	0.00%	0.04	590989502	0.01%	0.62%	227330289	0.93%	0.95%	0.06%	0.05%	210756346	105430712	13034808	5408940	291.04	108.00	0.66%	0.51%	0.97	0.000	563152554	0.8894	9999000000	231639433	Yes	0.00%				61.9%	85.2%	94.6%	279.5M	24.4%	97.4%	58688.3Mb	591.0M	53.6%	98.5%	88.9%
1173_K7E_T1_RNA_SLD_01_A23T55JLT4_1_1																																				101.7bp	97.9%	289.2M												82.4%	52%	151bp	151bp	27%	314.8M
1173_K7E_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		80.2%	53%	151bp	151bp	27%	314.8M
1173_K7E_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		82.0%	53%	102bp	97bp	18%	295.5M	82.0%	53%	102bp	97bp	18%	295.5M
1173_K7E_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		80.9%	53%	102bp	97bp	9%	295.5M	80.9%	53%	102bp	97bp	9%	295.5M
1173_K7E_T1_RNA_SLD_01_A23T55JLT4_2	104.9bp	100.0bp	0.01%	2.39%	0.60%	0.00%	0.01%	6.38%	0.89%	0.00%	0.03	430250586	0.01%	0.55%	191680581	0.93%	0.96%	0.05%	0.04%	178672994	89369555	10028999	4024021	252.91	94.00	0.64%	0.49%	0.97	0.000	409433344	0.9378	9999000000	207831911	No	0.00%				55.8%	84.6%	94.2%	202.7M	23.7%	97.3%	43687.8Mb	430.3M	54.3%	98.2%	87.6%
1173_K7E_T1_RNA_SLD_01_A23T55JLT4_2_1																																				104.9bp	98.0%	210.7M												80.0%	53%	151bp	151bp	27%	219.1M
1173_K7E_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		77.8%	53%	151bp	151bp	27%	219.1M
1173_K7E_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		79.9%	53%	104bp	102bp	18%	215.1M	79.9%	53%	104bp	102bp	18%	215.1M
1173_K7E_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		79.1%	54%	105bp	102bp	9%	215.1M	79.1%	54%	105bp	102bp	9%	215.1M
1173_KQG_T1_RNA_SLD_01_A23T55JLT4_1	115.7bp	107.0bp	0.01%	1.03%	0.36%	0.00%	0.03%	3.28%	0.59%	0.00%	0.07	293424850	0.01%	0.38%	180994090	0.95%	0.97%	0.04%	0.03%	172109925	86361409	7256995	2703846	279.55	135.00	0.73%	0.56%	0.97	0.000	282929273	0.9212	9999000000	402704147	No	0.00%				38.5%	88.1%	95.5%	140.1M	17.2%	97.2%	32126.5Mb	293.4M	54.3%	98.7%	77.8%
1173_KQG_T1_RNA_SLD_01_A23T55JLT4_1_1																																				115.7bp	98.5%	144.5M												77.8%	52%	151bp	151bp	27%	148.7M
1173_KQG_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		75.9%	53%	151bp	151bp	18%	148.7M
1173_KQG_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		77.9%	54%	112bp	112bp	18%	146.7M	77.9%	54%	112bp	112bp	18%	146.7M
1173_KQG_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		76.8%	54%	113bp	112bp	9%	146.7M	76.8%	54%	113bp	112bp	9%	146.7M
1173_KQG_T1_RNA_SLD_01_A23T55JLT4_2	123.4bp	115.0bp	0.01%	0.93%	0.35%	0.00%	0.02%	3.09%	0.58%	0.00%	0.04	591208916	0.01%	0.47%	314929297	0.95%	0.97%	0.04%	0.03%	297796094	149516984	13439273	4831027	510.10	276.00	0.79%	0.68%	0.97	0.000	570589299	0.9030	9999000000	502869640	Yes	0.00%				47.0%	88.6%	95.4%	282.0M	27.9%	97.2%	67925.6Mb	591.2M	54.7%	98.5%	73.0%
1173_KQG_T1_RNA_SLD_01_A23T55JLT4_2_1																																				123.4bp	98.5%	291.2M												83.0%	53%	151bp	151bp	27%	301.7M
1173_KQG_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		81.0%	54%	151bp	151bp	18%	301.7M
1173_KQG_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		83.0%	54%	118bp	122bp	18%	295.6M	83.0%	54%	118bp	122bp	18%	295.6M
1173_KQG_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		82.1%	54%	118bp	122bp	9%	295.6M	82.1%	54%	118bp	122bp	9%	295.6M
1173_KRT_T1_RNA_SLD_01_A23T55JLT4_1	105.7bp	99.0bp	0.01%	0.49%	0.28%	0.00%	0.01%	1.57%	0.52%	0.00%	4.61	20603440	0.01%	0.82%	3725974	0.81%	0.88%	0.13%	0.12%	3017713	1499405	467894	202906	3.91	1.00	0.02%	0.00%	0.97	0.000	18796807	0.8847	9999000000	3706642	No	0.00%				82.9%	70.5%	90.4%	9.3M	39.6%	96.8%	2061.6Mb	20.6M	60.5%	95.8%	85.5%
1173_KRT_T1_RNA_SLD_01_A23T55JLT4_1_1																																				105.7bp	96.2%	9.9M												83.0%	57%	151bp	151bp	36%	10.8M
1173_KRT_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		80.7%	58%	151bp	151bp	36%	10.8M
1173_KRT_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		82.8%	60%	103bp	102bp	18%	10.3M	82.8%	60%	103bp	102bp	18%	10.3M
1173_KRT_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		82.6%	60%	104bp	102bp	27%	10.3M	82.6%	60%	104bp	102bp	27%	10.3M
1173_KTN_T1_RNA_SLD_01_A23T55JLT4_1	101.0bp	96.0bp	0.03%	0.81%	0.33%	0.00%	0.05%	2.42%	0.51%	0.00%	0.03	592315872	0.01%	0.64%	210711939	0.92%	0.95%	0.06%	0.05%	194334244	97230901	12114806	5069492	264.38	115.00	0.69%	0.53%	0.97	0.000	565949722	0.8678	9999000000	196729125	No	0.00%				64.9%	86.5%	94.9%	281.1M	27.0%	97.4%	58797.0Mb	592.3M	54.0%	98.7%	89.6%
1173_KTN_T1_RNA_SLD_01_A23T55JLT4_1_1																																				101.0bp	98.2%	290.8M												83.4%	51%	151bp	151bp	27%	347.6M
1173_KTN_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		81.7%	53%	151bp	151bp	27%	347.6M
1173_KTN_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		82.3%	53%	102bp	97bp	18%	296.2M	82.3%	53%	102bp	97bp	18%	296.2M
1173_KTN_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		81.3%	54%	102bp	97bp	9%	296.2M	81.3%	54%	102bp	97bp	9%	296.2M
1173_KTN_T1_RNA_SLD_01_A23T55JLT4_2	106.2bp	101.0bp	0.01%	0.76%	0.33%	0.00%	0.03%	2.32%	0.51%	0.00%	0.03	442110440	0.01%	0.59%	182426653	0.93%	0.96%	0.05%	0.04%	169436934	84802795	9392061	3808598	241.25	107.00	0.68%	0.51%	0.98	0.000	422622925	0.9289	9999000000	175772744	No	0.00%				59.2%	86.3%	94.6%	209.2M	29.0%	97.5%	45903.3Mb	442.1M	54.4%	98.4%	87.1%
1173_KTN_T1_RNA_SLD_01_A23T55JLT4_2_1																																				106.2bp	98.3%	217.3M												80.3%	53%	151bp	151bp	27%	224.6M
1173_KTN_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		78.5%	54%	151bp	151bp	27%	224.6M
1173_KTN_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		80.2%	54%	106bp	102bp	18%	221.1M	80.2%	54%	106bp	102bp	18%	221.1M
1173_KTN_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		79.6%	54%	107bp	107bp	9%	221.1M	79.6%	54%	107bp	107bp	9%	221.1M
1173_LAF_T1_RNA_SLD_01_A23T55JLT4_1	84.8bp	81.0bp	0.03%	0.72%	0.30%	0.00%	0.06%	2.08%	0.46%	0.00%	4.78	91759492	0.02%	0.61%	35979849	0.87%	0.92%	0.09%	0.08%	31470843	15716389	3065383	1393814	35.54	11.00	0.31%	0.09%	0.97	0.000	69679484	0.8871	9999000000	35973504	No	0.00%				61.8%	31.7%	75.4%	34.6M	13.8%	97.1%	7177.2Mb	91.8M	56.8%	98.1%	96.3%
1173_LAF_T1_RNA_SLD_01_A23T55JLT4_1_1																																				84.8bp	81.1%	37.2M												75.4%	54%	151bp	151bp	27%	46.8M
1173_LAF_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		72.8%	56%	151bp	151bp	36%	46.8M
1173_LAF_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		75.6%	56%	80bp	77bp	27%	45.9M	75.6%	56%	80bp	77bp	27%	45.9M
1173_LAF_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		73.9%	57%	81bp	77bp	27%	45.9M	73.9%	57%	81bp	77bp	27%	45.9M
1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2	90.3bp	87.0bp	0.01%	0.68%	0.32%	0.00%	0.03%	2.02%	0.48%	0.00%	4.78	159812694	0.01%	0.65%	56347945	0.89%	0.94%	0.07%	0.06%	50362592	25137104	3932804	1703615	59.93	23.00	0.45%	0.18%	0.97	0.000	131088223	0.9363	9999000000	52965574	No	0.00%				65.6%	42.2%	81.4%	65.0M	17.2%	96.8%	13622.8Mb	159.8M	57.7%	97.9%	94.9%
1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2_1																																				90.3bp	87.3%	69.7M												80.2%	55%	151bp	151bp	27%	81.6M
1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		77.2%	56%	151bp	151bp	27%	81.6M
1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		80.3%	57%	88bp	87bp	27%	79.9M	80.3%	57%	88bp	87bp	27%	79.9M
1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		78.6%	58%	88bp	87bp	18%	79.9M	78.6%	58%	88bp	87bp	18%	79.9M
1173_LQD_T1_RNA_SLD_01_A23T55JLT4_1	119.2bp	111.0bp	0.02%	1.09%	0.45%	0.00%	0.03%	3.09%	0.68%	0.00%	6.59	589961126	0.01%	0.46%	318873301	0.94%	0.97%	0.04%	0.03%	300442468	150843920	13816700	4985519	495.30	227.00	0.72%	0.62%	0.97	0.000	566334435	0.9656	9999000000	525275414	No	0.00%				46.3%	86.7%	95.0%	280.1M	23.6%	97.2%	65819.6Mb	590.0M	54.3%	98.3%	76.8%
1173_LQD_T1_RNA_SLD_01_A23T55JLT4_1_1																																				119.2bp	98.2%	289.5M												85.7%	53%	151bp	151bp	27%	448.4M
1173_LQD_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		83.7%	54%	151bp	151bp	27%	448.4M
1173_LQD_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		83.4%	54%	115bp	117bp	18%	295.0M	83.4%	54%	115bp	117bp	18%	295.0M
1173_LQD_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		82.6%	54%	115bp	117bp	9%	295.0M	82.6%	54%	115bp	117bp	9%	295.0M
1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1	104.0bp	98.0bp	0.01%	0.55%	0.65%	0.00%	0.01%	1.74%	0.96%	0.00%	5.84	585545842	0.01%	0.52%	282979356	0.93%	0.96%	0.05%	0.04%	264297972	132144303	14155582	5571597	385.17	157.00	0.68%	0.57%	0.97	0.000	560762142	0.9198	9999000000	388096968	No	0.00%				52.1%	85.9%	94.6%	277.0M	21.7%	97.2%	59334.9Mb	585.5M	56.0%	97.6%	86.1%
1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1_1																																				104.0bp	98.3%	287.7M												85.9%	53%	151bp	151bp	27%	546.0M
1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		83.7%	55%	151bp	151bp	27%	546.0M
1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		82.0%	55%	104bp	102bp	18%	292.8M	82.0%	55%	104bp	102bp	18%	292.8M
1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		81.6%	56%	104bp	102bp	18%	292.8M	81.6%	56%	104bp	102bp	18%	292.8M
1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2	111.2bp	105.0bp	0.00%	0.49%	0.64%	0.00%	0.01%	1.57%	0.95%	0.00%	5.82	586606870	0.01%	0.52%	278808800	0.94%	0.96%	0.05%	0.04%	261495559	130841507	12789831	4850897	401.35	159.00	0.69%	0.57%	0.97	0.000	562428231	0.9548	9999000000	343297983	No	0.00%				52.9%	86.4%	94.8%	277.9M	25.3%	97.2%	62701.4Mb	586.6M	56.3%	97.8%	82.5%
1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2_1																																				111.2bp	98.5%	288.9M												83.9%	53%	151bp	151bp	27%	339.0M
1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		82.0%	55%	151bp	151bp	27%	339.0M
1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		83.0%	55%	110bp	107bp	18%	293.3M	83.0%	55%	110bp	107bp	18%	293.3M
1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		82.8%	56%	110bp	107bp	9%	293.3M	82.8%	56%	110bp	107bp	9%	293.3M
1173_M2F_T1_RNA_SLD_01_A23T55JLT4_1	98.6bp	92.0bp	0.03%	0.97%	0.28%	0.00%	0.06%	2.78%	0.44%	0.00%	0.03	467330428	0.01%	0.57%	202770699	0.93%	0.95%	0.05%	0.05%	188942467	94565774	10986760	4543894	258.78	116.00	0.70%	0.53%	0.98	0.000	446921361	0.8943	9999000000	216262817	No	0.00%				57.0%	86.3%	95.1%	222.1M	19.9%	97.5%	45241.1Mb	467.3M	53.8%	98.8%	89.1%
1173_M2F_T1_RNA_SLD_01_A23T55JLT4_1_1																																				98.6bp	98.2%	229.4M												82.1%	51%	151bp	151bp	27%	236.6M
1173_M2F_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		79.9%	53%	151bp	151bp	27%	236.6M
1173_M2F_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		82.1%	53%	99bp	97bp	18%	233.7M	82.1%	53%	99bp	97bp	18%	233.7M
1173_M2F_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		80.7%	54%	99bp	97bp	9%	233.7M	80.7%	54%	99bp	97bp	9%	233.7M
1173_M4Y_T1_RNA_SLD_01_A23T55JLT4_1	94.6bp	91.0bp	0.01%	1.09%	0.30%	0.00%	0.01%	3.08%	0.50%	0.00%	3.38	295889206	0.01%	0.76%	69815439	0.86%	0.91%	0.10%	0.09%	59737320	29716829	6987108	3042190	69.99	20.00	0.43%	0.19%	0.97	0.000	274853771	0.8893	9999000000	65381299	No	0.00%				77.2%	74.9%	91.7%	135.6M	28.4%	96.9%	27453.4Mb	295.9M	61.3%	97.1%	93.0%
1173_M4Y_T1_RNA_SLD_01_A23T55JLT4_1_1																																				94.6bp	96.4%	142.7M												84.8%	56%	151bp	151bp	27%	152.3M
1173_M4Y_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		82.2%	59%	151bp	151bp	36%	152.3M
1173_M4Y_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		84.8%	60%	95bp	92bp	18%	147.9M	84.8%	60%	95bp	92bp	18%	147.9M
1173_M4Y_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		84.3%	61%	96bp	92bp	9%	147.9M	84.3%	61%	96bp	92bp	9%	147.9M
1173_M5U_T1_RNA_SLD_01_A23T55JLT4_1	109.4bp	103.0bp	0.02%	0.86%	0.43%	0.00%	0.04%	2.47%	0.65%	0.00%	0.02	589768340	0.01%	0.52%	280945609	0.94%	0.96%	0.05%	0.04%	263663185	132100745	13209876	5113660	397.84	186.00	0.73%	0.60%	0.97	0.000	560615863	0.8958	9999000000	348197660	No	0.00%				52.7%	84.5%	94.1%	277.6M	24.5%	97.3%	62412.2Mb	589.8M	55.0%	98.3%	83.6%
1173_M5U_T1_RNA_SLD_01_A23T55JLT4_1_1																																				109.4bp	97.9%	288.8M												85.4%	53%	151bp	151bp	27%	459.3M
1173_M5U_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		84.2%	54%	151bp	151bp	27%	459.3M
1173_M5U_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		82.6%	54%	109bp	107bp	18%	294.9M	82.6%	54%	109bp	107bp	18%	294.9M
1173_M5U_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		82.4%	55%	109bp	107bp	9%	294.9M	82.4%	55%	109bp	107bp	9%	294.9M
1173_M5U_T1_RNA_SLD_01_A23T55JLT4_2	114.2bp	107.0bp	0.01%	0.80%	0.43%	0.00%	0.02%	2.33%	0.65%	0.00%	0.01	572460602	0.01%	0.58%	239783156	0.94%	0.96%	0.05%	0.04%	224209117	112306068	11287289	4248796	343.38	158.00	0.71%	0.58%	0.97	0.000	542685554	0.9308	9999000000	228146487	No	0.00%				58.6%	83.6%	93.7%	268.2M	30.6%	97.2%	62281.0Mb	572.5M	55.7%	98.0%	81.3%
1173_M5U_T1_RNA_SLD_01_A23T55JLT4_2_1																																				114.2bp	97.9%	280.2M												84.3%	53%	151bp	151bp	27%	291.9M
1173_M5U_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		82.7%	54%	151bp	151bp	27%	291.9M
1173_M5U_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		84.3%	55%	112bp	112bp	18%	286.2M	84.3%	55%	112bp	112bp	18%	286.2M
1173_M5U_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		84.2%	55%	112bp	112bp	9%	286.2M	84.2%	55%	112bp	112bp	9%	286.2M
1173_MHK_T1_RNA_SLD_01_A23T55JLT4_1	114.2bp	109.0bp	0.01%	1.00%	0.36%	0.00%	0.03%	3.05%	0.60%	0.00%	4.82	588626252	0.01%	0.50%	293691175	0.93%	0.96%	0.05%	0.04%	273199718	136871135	15154457	5976458	424.64	211.00	0.75%	0.63%	0.97	0.000	556697901	0.9238	9999000000	360509690	No	0.00%				50.6%	82.8%	93.5%	275.2M	30.0%	97.2%	64169.0Mb	588.6M	55.4%	98.1%	81.4%
1173_MHK_T1_RNA_SLD_01_A23T55JLT4_1_1																																				114.2bp	98.0%	288.5M												85.7%	54%	151bp	151bp	27%	467.0M
1173_MHK_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		84.0%	55%	151bp	151bp	27%	467.0M
1173_MHK_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		82.7%	55%	112bp	112bp	18%	294.3M	82.7%	55%	112bp	112bp	18%	294.3M
1173_MHK_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		82.4%	55%	112bp	112bp	9%	294.3M	82.4%	55%	112bp	112bp	9%	294.3M
1173_N5V_T1_RNA_SLD_01_A23T55JLT4_1	99.5bp	94.0bp	0.01%	0.90%	0.41%	0.00%	0.02%	2.58%	0.56%	0.00%	4.95	402035990	0.01%	0.51%	195765111	0.94%	0.95%	0.05%	0.05%	183192529	91681520	10458857	4395656	250.11	105.00	0.67%	0.51%	0.97	0.000	383931747	0.9522	9999000000	238931848	No	0.00%				51.6%	86.4%	94.8%	190.5M	20.4%	97.4%	39273.0Mb	402.0M	52.5%	98.7%	90.0%
1173_N5V_T1_RNA_SLD_01_A23T55JLT4_1_1																																				99.5bp	98.3%	197.5M												81.7%	50%	151bp	151bp	27%	203.7M
1173_N5V_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		80.5%	52%	151bp	151bp	27%	203.7M
1173_N5V_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		81.7%	52%	100bp	97bp	18%	201.0M	81.7%	52%	100bp	97bp	18%	201.0M
1173_N5V_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		81.4%	52%	100bp	97bp	9%	201.0M	81.4%	52%	100bp	97bp	9%	201.0M
1173_NBF_T1_RNA_SLD_01_A23T55JLT4_1	105.6bp	99.0bp	0.02%	1.27%	0.50%	0.00%	0.04%	3.48%	0.69%	0.00%	0.02	471579430	0.01%	0.51%	232425031	0.94%	0.96%	0.05%	0.04%	217486620	108974062	11138397	4429033	316.01	140.00	0.70%	0.56%	0.98	0.000	449137063	0.9259	9999000000	303160188	No	0.00%				51.1%	85.5%	94.5%	222.9M	23.6%	97.4%	48034.0Mb	471.6M	52.4%	98.5%	86.0%
1173_NBF_T1_RNA_SLD_01_A23T55JLT4_1_1																																				105.6bp	98.3%	231.8M												82.5%	51%	151bp	151bp	27%	239.3M
1173_NBF_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		80.6%	53%	151bp	151bp	27%	239.3M
1173_NBF_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		82.5%	52%	104bp	102bp	18%	235.8M	82.5%	52%	104bp	102bp	18%	235.8M
1173_NBF_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		81.6%	52%	105bp	102bp	9%	235.8M	81.6%	52%	105bp	102bp	9%	235.8M
1173_NFO_T1_RNA_SLD_01_A23T55JLT4_1	100.8bp	96.0bp	0.01%	0.55%	0.26%	0.00%	0.02%	1.73%	0.44%	0.00%	0.01	589236474	0.01%	0.76%	140201276	0.88%	0.92%	0.08%	0.08%	123229970	61550629	11672551	5055298	160.07	59.00	0.62%	0.39%	0.97	0.000	559215974	0.8645	9999000000	119557007	No	0.00%				76.9%	82.8%	93.9%	276.6M	34.1%	97.2%	58063.8Mb	589.2M	59.3%	98.2%	90.3%
1173_NFO_T1_RNA_SLD_01_A23T55JLT4_1_1																																				100.8bp	97.7%	287.9M												87.8%	55%	151bp	151bp	27%	324.0M
1173_NFO_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		85.9%	57%	151bp	151bp	27%	324.0M
1173_NFO_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		87.3%	59%	101bp	97bp	18%	294.6M	87.3%	59%	101bp	97bp	18%	294.6M
1173_NFO_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		86.7%	59%	102bp	97bp	9%	294.6M	86.7%	59%	102bp	97bp	9%	294.6M
1173_NFO_T1_RNA_SLD_01_A23T55JLT4_2	105.2bp	100.0bp	0.01%	0.51%	0.25%	0.00%	0.01%	1.61%	0.43%	0.00%	0.01	434487944	0.01%	0.77%	98726812	0.88%	0.93%	0.08%	0.07%	86451150	43152123	8025189	3387362	115.72	42.00	0.57%	0.32%	0.97	0.000	410656996	0.9276	9999000000	82458225	No	0.00%				78.0%	81.3%	93.4%	202.9M	47.7%	97.1%	44428.9Mb	434.5M	59.5%	97.7%	87.1%
1173_NFO_T1_RNA_SLD_01_A23T55JLT4_2_1																																				105.2bp	97.6%	212.1M												87.5%	56%	151bp	151bp	27%	222.5M
1173_NFO_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		85.3%	57%	151bp	151bp	27%	222.5M
1173_NFO_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		87.5%	59%	105bp	102bp	18%	217.2M	87.5%	59%	105bp	102bp	18%	217.2M
1173_NFO_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		87.2%	59%	106bp	102bp	9%	217.2M	87.2%	59%	106bp	102bp	9%	217.2M
1173_NMN_T1_RNA_SLD_01_A23T55JLT4_1	111.6bp	104.0bp	0.01%	1.16%	0.33%	0.00%	0.01%	3.56%	0.56%	0.00%	0.05	535830628	0.01%	0.48%	278883915	0.93%	0.96%	0.05%	0.04%	259798634	130015804	14656810	5708534	393.09	197.00	0.75%	0.62%	0.97	0.000	508142201	0.9321	9999000000	365493050	Yes	0.00%				48.4%	83.3%	93.8%	251.2M	22.3%	97.1%	56493.5Mb	535.8M	56.2%	97.9%	82.7%
1173_NMN_T1_RNA_SLD_01_A23T55JLT4_1_1																																				111.6bp	98.1%	262.7M												81.5%	54%	151bp	151bp	27%	273.8M
1173_NMN_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		79.4%	55%	151bp	151bp	27%	273.8M
1173_NMN_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		81.5%	55%	108bp	107bp	18%	267.9M	81.5%	55%	108bp	107bp	18%	267.9M
1173_NMN_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		81.0%	56%	109bp	107bp	9%	267.9M	81.0%	56%	109bp	107bp	9%	267.9M
1173_NMN_T1_RNA_SLD_01_A23T55JLT4_2	115.4bp	109.0bp	0.01%	1.02%	0.30%	0.00%	0.01%	3.21%	0.52%	0.00%	0.04	515542990	0.01%	0.51%	250240209	0.93%	0.96%	0.05%	0.04%	233206467	116733684	12484451	4698039	362.71	175.00	0.74%	0.60%	0.97	0.000	488491029	0.9650	9999000000	278828175	No	0.00%				51.9%	83.5%	93.7%	241.5M	28.3%	97.2%	56144.6Mb	515.5M	56.6%	97.6%	80.2%
1173_NMN_T1_RNA_SLD_01_A23T55JLT4_2_1																																				115.4bp	98.3%	253.4M												83.1%	54%	151bp	151bp	27%	264.0M
1173_NMN_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		80.7%	56%	151bp	151bp	27%	264.0M
1173_NMN_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		83.1%	56%	112bp	112bp	18%	257.8M	83.1%	56%	112bp	112bp	18%	257.8M
1173_NMN_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		82.6%	56%	112bp	112bp	9%	257.8M	82.6%	56%	112bp	112bp	9%	257.8M
1173_NWE_T1_RNA_SLD_01_A23T55JLT4_1	120.7bp	110.0bp	0.03%	1.56%	0.57%	0.00%	0.05%	4.51%	0.84%	0.00%	0.02	591985946	0.01%	0.46%	321016154	0.95%	0.97%	0.04%	0.03%	304251725	152890536	13406060	4726635	523.42	219.00	0.74%	0.62%	0.97	0.000	571753100	0.9475	9999000000	613619665	Yes	0.09%				46.0%	89.0%	95.7%	283.4M	22.8%	97.4%	66686.0Mb	592.0M	53.3%	98.7%	72.6%
1173_NWE_T1_RNA_SLD_01_A23T55JLT4_1_1																																				120.7bp	98.4%	291.3M												87.3%	51%	151bp	151bp	27%	647.6M
1173_NWE_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		85.6%	53%	151bp	151bp	27%	647.6M
1173_NWE_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		83.1%	52%	116bp	117bp	18%	296.0M	83.1%	52%	116bp	117bp	18%	296.0M
1173_NWE_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		82.2%	53%	116bp	117bp	9%	296.0M	82.2%	53%	116bp	117bp	9%	296.0M
1173_NWE_T1_RNA_SLD_01_A23T55JLT4_2	127.1bp	117.0bp	0.01%	1.49%	0.57%	0.00%	0.03%	4.39%	0.85%	0.00%	0.03	590798576	0.01%	0.44%	330995036	0.95%	0.97%	0.04%	0.03%	313769013	157726980	13661349	4686245	558.33	244.00	0.74%	0.63%	0.97	0.000	571456217	0.9708	9999000000	625077298	Yes	0.11%				44.2%	89.7%	95.7%	282.7M	25.5%	97.3%	68997.7Mb	590.8M	53.3%	98.5%	69.2%
1173_NWE_T1_RNA_SLD_01_A23T55JLT4_2_1																																				127.1bp	98.4%	290.7M												87.2%	52%	151bp	151bp	27%	609.9M
1173_NWE_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		85.5%	53%	151bp	151bp	18%	609.9M
1173_NWE_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		83.3%	52%	120bp	122bp	18%	295.4M	83.3%	52%	120bp	122bp	18%	295.4M
1173_NWE_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		82.6%	53%	120bp	122bp	9%	295.4M	82.6%	53%	120bp	122bp	9%	295.4M
1173_OHF_T1_RNA_SLD_01_A23T55JLT4_1	92.7bp	90.0bp	0.01%	0.34%	0.19%	0.00%	0.01%	1.05%	0.29%	0.00%	0.20	8725292	0.01%	0.31%	6006273	0.92%	0.94%	0.06%	0.06%	5539565	2769321	367462	164018	6.73	2.00	0.05%	0.01%	0.97	0.000	8223018	0.8410	9999000000	10154472	Skipped	N/A%				31.5%	81.5%	93.6%	4.1M	10.8%	97.4%	789.5Mb	8.7M	57.3%	97.9%	94.8%
1173_OHF_T1_RNA_SLD_01_A23T55JLT4_1_1																																				92.7bp	97.0%	4.2M												45.1%	54%	151bp	151bp	18%	4.5M
1173_OHF_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		41.3%	55%	151bp	151bp	27%	4.5M
1173_OHF_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		44.7%	57%	93bp	92bp	9%	4.4M	44.7%	57%	93bp	92bp	9%	4.4M
1173_OHF_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		41.5%	57%	93bp	92bp	0%	4.4M	41.5%	57%	93bp	92bp	0%	4.4M
1173_PCB_T1_RNA_SLD_01_A23T55JLT4_1	106.3bp	100.0bp	0.01%	1.18%	0.28%	0.00%	0.03%	3.60%	0.47%	0.00%	0.31	210238966	0.01%	0.36%	135598308	0.94%	0.96%	0.05%	0.04%	127926692	64036228	6205100	2546288	184.43	82.00	0.66%	0.46%	0.98	0.000	201087381	0.9169	9999000000	268691964	No	0.00%				35.8%	86.4%	94.8%	99.6M	12.7%	97.4%	21370.7Mb	210.2M	53.7%	98.5%	86.8%
1173_PCB_T1_RNA_SLD_01_A23T55JLT4_1_1																																				106.3bp	98.0%	103.0M												68.5%	51%	151bp	151bp	27%	106.8M
1173_PCB_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		66.6%	53%	151bp	151bp	27%	106.8M
1173_PCB_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		68.4%	53%	104bp	102bp	18%	105.1M	68.4%	53%	104bp	102bp	18%	105.1M
1173_PCB_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		67.5%	53%	105bp	102bp	9%	105.1M	67.5%	53%	105bp	102bp	9%	105.1M
1173_PCB_T1_RNA_SLD_01_A23T55JLT4_2	113.4bp	107.0bp	0.01%	1.06%	0.27%	0.00%	0.01%	3.36%	0.47%	0.00%	0.43	461947406	0.01%	0.48%	239685830	0.94%	0.96%	0.05%	0.04%	224187033	112237018	11771296	4693910	340.05	171.00	0.74%	0.60%	0.97	0.000	441514673	0.9546	9999000000	291940231	Yes	0.65%				48.5%	86.2%	94.5%	218.2M	23.4%	97.3%	49590.1Mb	461.9M	54.4%	98.1%	82.7%
1173_PCB_T1_RNA_SLD_01_A23T55JLT4_2_1																																				113.4bp	98.1%	226.7M												76.5%	52%	151bp	151bp	27%	235.3M
1173_PCB_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		74.9%	54%	151bp	151bp	18%	235.3M
1173_PCB_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		76.5%	54%	110bp	107bp	18%	231.0M	76.5%	54%	110bp	107bp	18%	231.0M
1173_PCB_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		76.3%	54%	110bp	107bp	9%	231.0M	76.3%	54%	110bp	107bp	9%	231.0M
1173_Q7O_T1_RNA_SLD_01_A23T55JLT4_1	110.5bp	104.0bp	0.01%	0.96%	0.33%	0.00%	0.01%	2.92%	0.50%	0.00%	5.64	588936476	0.01%	0.48%	306003141	0.93%	0.96%	0.05%	0.04%	285418469	143003221	16133887	6470161	443.17	204.00	0.72%	0.61%	0.97	0.000	565691045	0.9267	9999000000	463301398	Yes	0.02%				48.4%	86.8%	94.8%	279.2M	23.2%	97.3%	63098.8Mb	588.9M	53.8%	98.2%	81.3%
1173_Q7O_T1_RNA_SLD_01_A23T55JLT4_1_1																																				110.5bp	98.0%	288.7M												84.8%	52%	151bp	151bp	27%	451.2M
1173_Q7O_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		83.4%	53%	151bp	151bp	27%	451.2M
1173_Q7O_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		82.3%	53%	110bp	107bp	18%	294.5M	82.3%	53%	110bp	107bp	18%	294.5M
1173_Q7O_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		82.2%	54%	110bp	107bp	9%	294.5M	82.2%	54%	110bp	107bp	9%	294.5M
1173_Q7O_T1_RNA_SLD_01_A23T55JLT4_2	116.6bp	109.0bp	0.00%	0.91%	0.32%	0.00%	0.01%	2.83%	0.48%	0.00%	5.71	493511196	0.01%	0.47%	262255556	0.94%	0.96%	0.05%	0.04%	245525383	122929749	12604136	4782767	393.83	180.00	0.71%	0.59%	0.97	0.000	473677818	0.9450	9999000000	369292620	Yes	0.00%				47.3%	86.7%	94.5%	233.3M	24.5%	97.2%	54888.1Mb	493.5M	53.8%	97.9%	78.2%
1173_Q7O_T1_RNA_SLD_01_A23T55JLT4_2_1																																				116.6bp	98.3%	242.5M												82.3%	52%	151bp	151bp	27%	252.0M
1173_Q7O_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		80.1%	53%	151bp	151bp	18%	252.0M
1173_Q7O_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		82.2%	53%	114bp	112bp	18%	246.8M	82.2%	53%	114bp	112bp	18%	246.8M
1173_Q7O_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		81.7%	54%	115bp	117bp	9%	246.8M	81.7%	54%	115bp	117bp	9%	246.8M
1173_QEV_T1_RNA_SLD_01_A23T55JLT4_1	109.1bp	104.0bp	0.00%	0.47%	0.25%	0.00%	0.00%	1.59%	0.51%	0.00%	5.13	19648442	0.01%	0.83%	3266824	0.78%	0.86%	0.14%	0.14%	2556621	1269175	467035	205977	3.33	1.00	0.01%	0.00%	0.97	0.000	18193017	0.8699	9999000000	4302976	Skipped	N/A%				84.4%	76.3%	91.7%	9.0M	43.6%	96.6%	2038.0Mb	19.6M	61.1%	94.2%	83.8%
1173_QEV_T1_RNA_SLD_01_A23T55JLT4_1_1																																				109.1bp	96.5%	9.5M												83.8%	58%	151bp	151bp	36%	10.4M
1173_QEV_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		81.6%	60%	151bp	151bp	36%	10.4M
1173_QEV_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		83.3%	60%	107bp	107bp	18%	9.8M	83.3%	60%	107bp	107bp	18%	9.8M
1173_QEV_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		83.1%	61%	108bp	107bp	18%	9.8M	83.1%	61%	108bp	107bp	18%	9.8M
1173_QJL_T1_RNA_SLD_01_A23T55JLT4_1	105.5bp	99.0bp	0.08%	1.30%	0.31%	0.00%	0.16%	3.64%	0.47%	0.00%	5.83	309884076	0.01%	0.44%	174638594	0.94%	0.96%	0.05%	0.04%	164460902	82387027	8180976	3254340	238.60	102.00	0.68%	0.50%	0.97	0.000	295641761	0.8998	9999000000	273760601	No	0.00%				43.9%	85.1%	94.7%	146.7M	18.4%	97.4%	31492.9Mb	309.9M	54.6%	98.4%	86.2%
1173_QJL_T1_RNA_SLD_01_A23T55JLT4_1_1																																				105.5bp	98.2%	152.2M												77.2%	53%	151bp	151bp	27%	157.4M
1173_QJL_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		74.9%	54%	151bp	151bp	27%	157.4M
1173_QJL_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		77.2%	54%	104bp	102bp	18%	154.9M	77.2%	54%	104bp	102bp	18%	154.9M
1173_QJL_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		75.9%	54%	105bp	102bp	9%	154.9M	75.9%	54%	105bp	102bp	9%	154.9M
1173_QJL_T1_RNA_SLD_01_A23T55JLT4_2	109.5bp	103.0bp	0.05%	1.21%	0.31%	0.00%	0.09%	3.47%	0.48%	0.00%	5.81	478513910	0.01%	0.51%	236609189	0.94%	0.96%	0.05%	0.04%	221534390	110986634	11573993	4494417	330.54	151.00	0.72%	0.58%	0.98	0.000	456017646	0.9352	9999000000	282429261	No	0.00%				50.9%	84.4%	94.3%	225.5M	23.5%	97.4%	50393.8Mb	478.5M	55.8%	98.2%	83.5%
1173_QJL_T1_RNA_SLD_01_A23T55JLT4_2_1																																				109.5bp	98.2%	235.0M												80.8%	53%	151bp	151bp	27%	243.7M
1173_QJL_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		78.8%	55%	151bp	151bp	27%	243.7M
1173_QJL_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		80.8%	55%	108bp	107bp	18%	239.3M	80.8%	55%	108bp	107bp	18%	239.3M
1173_QJL_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		80.1%	56%	108bp	107bp	9%	239.3M	80.1%	56%	108bp	107bp	9%	239.3M
1173_RDM_T1_RNA_SLD_01_A23T55JLT4_1	108.9bp	106.0bp	0.01%	0.89%	0.35%	0.00%	0.01%	2.72%	0.60%	0.00%	0.02	552270522	0.01%	0.79%	117615979	0.86%	0.92%	0.09%	0.08%	101336332	50591550	10415135	4513879	136.82	48.00	0.59%	0.34%	0.97	0.000	523078199	0.8721	9990000000	97946803	No	0.00%				79.5%	82.3%	93.4%	257.8M	43.3%	97.1%	58435.8Mb	552.3M	59.6%	98.0%	86.7%
1173_RDM_T1_RNA_SLD_01_A23T55JLT4_1_1																																				108.9bp	97.5%	269.1M												86.7%	56%	151bp	151bp	27%	281.7M
1173_RDM_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		84.8%	58%	151bp	151bp	18%	281.7M
1173_RDM_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		86.8%	59%	109bp	107bp	18%	276.1M	86.8%	59%	109bp	107bp	18%	276.1M
1173_RDM_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		86.3%	59%	109bp	107bp	9%	276.1M	86.3%	59%	109bp	107bp	9%	276.1M
1173_RDM_T1_RNA_SLD_01_A23T55JLT4_2	103.1bp	99.0bp	0.01%	0.87%	0.35%	0.00%	0.01%	2.64%	0.60%	0.00%	0.03	274799746	0.01%	0.76%	66574347	0.87%	0.92%	0.09%	0.08%	57602821	28751337	5791518	2517891	74.98	25.00	0.47%	0.20%	0.97	0.000	258447503	0.9436	9999000000	56065518	No	0.00%				76.7%	80.7%	92.9%	127.6M	39.7%	97.1%	27679.5Mb	274.8M	59.8%	97.8%	89.2%
1173_RDM_T1_RNA_SLD_01_A23T55JLT4_2_1																																				103.1bp	97.1%	133.5M												84.4%	56%	151bp	151bp	27%	140.4M
1173_RDM_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		82.5%	57%	151bp	151bp	27%	140.4M
1173_RDM_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		84.5%	59%	103bp	102bp	18%	137.4M	84.5%	59%	103bp	102bp	18%	137.4M
1173_RDM_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		84.2%	60%	104bp	102bp	9%	137.4M	84.2%	60%	104bp	102bp	9%	137.4M
1173_RGW_T1_RNA_SLD_01_A23T55JLT4_1	94.3bp	92.0bp	0.00%	0.37%	0.24%	0.00%	0.00%	1.28%	0.51%	0.00%	0.25	215545380	0.02%	0.85%	31903297	0.76%	0.86%	0.13%	0.14%	24266103	12059699	4245914	1910350	28.05	4.00	0.19%	0.06%	0.97	0.000	193902544	0.8914	9999000000	32719055	No	0.00%				86.6%	67.2%	88.5%	95.3M	38.5%	96.7%	20064.7Mb	215.5M	65.5%	97.5%	92.9%
1173_RGW_T1_RNA_SLD_01_A23T55JLT4_1_1																																				94.3bp	95.0%	102.4M												88.1%	60%	151bp	151bp	27%	110.5M
1173_RGW_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		85.8%	61%	151bp	151bp	27%	110.5M
1173_RGW_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		88.2%	65%	96bp	92bp	18%	107.8M	88.2%	65%	96bp	92bp	18%	107.8M
1173_RGW_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		87.6%	65%	97bp	97bp	9%	107.8M	87.6%	65%	97bp	97bp	9%	107.8M
1173_RPC_T1_RNA_SLD_01_A23T55JLT4_1	100.4bp	95.0bp	0.02%	1.45%	0.60%	0.00%	0.04%	4.02%	0.84%	0.00%	0.01	54903478	0.01%	0.28%	39647624	0.95%	0.97%	0.04%	0.03%	37690621	18870104	1599203	647867	51.50	15.00	0.38%	0.14%	0.97	0.000	52596346	0.9091	9999000000	104874615	No	0.00%				28.0%	86.8%	95.2%	26.1M	11.7%	97.4%	5361.9Mb	54.9M	53.0%	98.5%	90.4%
1173_RPC_T1_RNA_SLD_01_A23T55JLT4_1_1																																				100.4bp	98.3%	27.0M												60.8%	51%	151bp	151bp	27%	27.9M
1173_RPC_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		57.4%	53%	151bp	151bp	27%	27.9M
1173_RPC_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		60.7%	52%	100bp	97bp	18%	27.5M	60.7%	52%	100bp	97bp	18%	27.5M
1173_RPC_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		58.0%	53%	100bp	97bp	9%	27.5M	58.0%	53%	100bp	97bp	9%	27.5M
1173_RPC_T1_RNA_SLD_01_A23T55JLT4_2	102.5bp	98.0bp	0.01%	1.38%	0.62%	0.00%	0.02%	3.94%	0.88%	0.00%	0.01	559664924	0.01%	0.63%	206892507	0.93%	0.96%	0.05%	0.04%	192898054	96486050	10041529	3973160	268.78	108.00	0.65%	0.51%	0.98	0.000	536486948	0.9562	9999000000	183787634	No	0.00%				63.5%	87.0%	95.0%	265.9M	28.5%	97.4%	56211.9Mb	559.7M	53.7%	98.3%	88.9%
1173_RPC_T1_RNA_SLD_01_A23T55JLT4_2_1																																				102.5bp	98.5%	275.6M												83.7%	51%	151bp	151bp	27%	284.7M
1173_RPC_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		81.8%	53%	151bp	151bp	27%	284.7M
1173_RPC_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		83.8%	53%	103bp	102bp	18%	279.8M	83.8%	53%	103bp	102bp	18%	279.8M
1173_RPC_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		83.1%	53%	103bp	102bp	9%	279.8M	83.1%	53%	103bp	102bp	9%	279.8M
1173_RPX_T1_RNA_SLD_01_A23T55JLT4_1	111.6bp	106.0bp	0.02%	1.13%	0.50%	0.00%	0.04%	3.31%	0.77%	0.00%	4.13	589673654	0.01%	0.52%	285971663	0.94%	0.97%	0.04%	0.03%	269183503	134862052	12628217	4780866	414.43	177.00	0.71%	0.59%	0.97	0.000	562755445	0.9384	9999000000	370211139	No	0.00%				51.9%	85.0%	94.3%	278.2M	25.8%	97.2%	63801.1Mb	589.7M	55.4%	98.3%	82.6%
1173_RPX_T1_RNA_SLD_01_A23T55JLT4_1_1																																				111.6bp	98.4%	290.1M												84.3%	53%	151bp	151bp	27%	359.3M
1173_RPX_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		82.3%	55%	151bp	151bp	27%	359.3M
1173_RPX_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		83.1%	55%	111bp	112bp	18%	294.8M	83.1%	55%	111bp	112bp	18%	294.8M
1173_RPX_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		82.3%	55%	111bp	112bp	9%	294.8M	82.3%	55%	111bp	112bp	9%	294.8M
1173_RQT_T1_RNA_SLD_01_A23T55JLT4_1	102.0bp	97.0bp	0.01%	0.94%	0.56%	0.00%	0.01%	2.89%	0.88%	0.00%	5.30	535352092	0.01%	0.72%	147337300	0.89%	0.93%	0.07%	0.07%	131751725	65723092	11045198	4629182	173.60	65.00	0.62%	0.41%	0.97	0.000	510001679	0.8748	9999000000	129033287	No	0.00%				73.1%	83.4%	94.0%	251.6M	34.1%	97.2%	53711.9Mb	535.4M	58.5%	97.5%	88.3%
1173_RQT_T1_RNA_SLD_01_A23T55JLT4_1_1																																				102.0bp	97.8%	261.8M												85.8%	54%	151bp	151bp	27%	274.6M
1173_RQT_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		83.8%	56%	151bp	151bp	27%	274.6M
1173_RQT_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		85.7%	58%	103bp	102bp	18%	267.7M	85.7%	58%	103bp	102bp	18%	267.7M
1173_RQT_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		85.7%	58%	103bp	102bp	18%	267.7M	85.7%	58%	103bp	102bp	18%	267.7M
1173_RQT_T1_RNA_SLD_01_A23T55JLT4_2	106.8bp	102.0bp	0.01%	0.87%	0.54%	0.00%	0.01%	2.69%	0.86%	0.00%	5.36	381193030	0.01%	0.77%	88468794	0.88%	0.93%	0.08%	0.07%	78082724	38975830	7045580	2970596	106.41	37.00	0.54%	0.29%	0.97	0.000	363311467	0.9199	9999000000	77981001	No	0.00%				77.5%	83.7%	94.1%	179.3M	40.1%	97.0%	39658.6Mb	381.2M	58.4%	97.7%	86.3%
1173_RQT_T1_RNA_SLD_01_A23T55JLT4_2_1																																				106.8bp	97.8%	186.4M												86.5%	55%	151bp	151bp	27%	195.0M
1173_RQT_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		84.6%	57%	151bp	151bp	27%	195.0M
1173_RQT_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		86.6%	58%	107bp	107bp	18%	190.6M	86.6%	58%	107bp	107bp	18%	190.6M
1173_RQT_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		86.5%	58%	107bp	107bp	9%	190.6M	86.5%	58%	107bp	107bp	9%	190.6M
1173_S9Q_T1_RNA_SLD_01_A23T55JLT4_1	115.2bp	110.0bp	0.01%	0.68%	0.38%	0.00%	0.01%	2.13%	0.60%	0.00%	5.30	589434978	0.01%	0.58%	246906381	0.93%	0.96%	0.05%	0.04%	229686848	115107068	12182822	4705661	354.68	156.00	0.73%	0.58%	0.97	0.000	561190309	0.9135	9999000000	238611394	No	0.00%				58.6%	84.4%	94.1%	277.4M	35.4%	97.1%	64801.8Mb	589.4M	57.8%	98.2%	81.1%
1173_S9Q_T1_RNA_SLD_01_A23T55JLT4_1_1																																				115.2bp	98.1%	289.2M												84.7%	56%	151bp	151bp	27%	303.9M
1173_S9Q_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		82.8%	56%	151bp	151bp	18%	303.9M
1173_S9Q_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		84.7%	57%	113bp	112bp	18%	294.7M	84.7%	57%	113bp	112bp	18%	294.7M
1173_S9Q_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		84.1%	58%	113bp	112bp	9%	294.7M	84.1%	58%	113bp	112bp	9%	294.7M
1173_SDP_T1_RNA_SLD_01_A23T55JLT4_1	98.9bp	95.0bp	0.01%	0.63%	0.38%	0.00%	0.01%	2.03%	0.61%	0.00%	0.01	591408718	0.01%	0.69%	183715625	0.92%	0.95%	0.06%	0.05%	169248223	84603070	10390326	4332168	225.66	95.00	0.68%	0.49%	0.97	0.000	567832504	0.9137	9999000000	146237655	No	0.00%				69.4%	86.8%	95.2%	281.6M	30.3%	97.3%	58231.4Mb	591.4M	56.5%	98.6%	91.0%
1173_SDP_T1_RNA_SLD_01_A23T55JLT4_1_1																																				98.9bp	98.5%	291.2M												86.8%	53%	151bp	151bp	27%	363.2M
1173_SDP_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		85.1%	55%	151bp	151bp	27%	363.2M
1173_SDP_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		85.5%	56%	101bp	97bp	18%	295.7M	85.5%	56%	101bp	97bp	18%	295.7M
1173_SDP_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		84.8%	56%	101bp	97bp	9%	295.7M	84.8%	56%	101bp	97bp	9%	295.7M
1173_SFA_T1_RNA_SLD_01_A23T55JLT4_1	133.0bp	123.0bp	0.01%	0.66%	0.24%	0.00%	0.01%	2.31%	0.48%	0.00%	0.05	69311912	0.01%	0.88%	8171081	0.79%	0.87%	0.13%	0.13%	6477432	3228521	1095399	464260	10.28	3.00	0.08%	0.01%	0.97	0.000	64382193	0.9240	9999000000	9380287	No	0.00%				89.0%	78.3%	92.1%	31.9M	60.7%	97.0%	8164.5Mb	69.3M	58.6%	97.2%	68.3%
1173_SFA_T1_RNA_SLD_01_A23T55JLT4_1_1																																				133.0bp	97.6%	33.8M												88.6%	56%	151bp	151bp	27%	35.7M
1173_SFA_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		86.3%	58%	151bp	151bp	27%	35.7M
1173_SFA_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		88.6%	58%	121bp	127bp	9%	34.7M	88.6%	58%	121bp	127bp	9%	34.7M
1173_SFA_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		88.1%	58%	122bp	127bp	9%	34.7M	88.1%	58%	122bp	127bp	9%	34.7M
1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1	114.2bp	107.0bp	0.03%	1.11%	0.42%	0.00%	0.05%	3.15%	0.63%	0.00%	5.79	590148122	0.01%	0.47%	310107163	0.94%	0.96%	0.05%	0.04%	291351430	146141971	14186064	5324019	467.32	239.00	0.78%	0.65%	0.97	0.000	561485209	0.9185	9999000000	488366428	No	0.00%				47.8%	83.7%	94.3%	278.3M	22.6%	97.2%	63881.5Mb	590.1M	54.5%	98.4%	78.8%
1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1_1																																				114.2bp	97.9%	288.8M												84.6%	53%	151bp	151bp	27%	372.8M
1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		82.6%	54%	151bp	151bp	27%	372.8M
1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		83.2%	54%	111bp	112bp	18%	295.1M	83.2%	54%	111bp	112bp	18%	295.1M
1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		82.4%	54%	112bp	112bp	9%	295.1M	82.4%	54%	112bp	112bp	9%	295.1M
1173_SK8_T1_RNA_SLD_01_A23T55JLT4_2	126.0bp	117.0bp	0.01%	1.02%	0.41%	0.00%	0.03%	2.99%	0.62%	0.00%	5.82	408413764	0.01%	0.41%	241890484	0.94%	0.97%	0.04%	0.03%	228387373	114649757	10051521	3513201	390.57	198.00	0.76%	0.63%	0.97	0.000	390110458	0.9540	9999000000	410428532	No	0.00%				41.1%	86.1%	94.5%	192.9M	22.7%	97.2%	47239.5Mb	408.4M	55.1%	98.1%	72.3%
1173_SK8_T1_RNA_SLD_01_A23T55JLT4_2_1																																				126.0bp	98.1%	200.3M												81.1%	53%	151bp	151bp	27%	208.2M
1173_SK8_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		78.8%	55%	151bp	151bp	18%	208.2M
1173_SK8_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		81.2%	54%	119bp	122bp	18%	204.2M	81.2%	54%	119bp	122bp	18%	204.2M
1173_SK8_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		80.3%	55%	119bp	122bp	9%	204.2M	80.3%	55%	119bp	122bp	9%	204.2M
1173_T46_T1_RNA_SLD_01_A23T55JLT4_1	108.6bp	103.0bp	0.00%	0.98%	0.29%	0.00%	0.01%	3.06%	0.48%	0.00%	5.92	590630284	0.01%	0.76%	139724981	0.90%	0.94%	0.07%	0.06%	125745207	62898399	9435267	3860586	176.14	53.00	0.59%	0.38%	0.97	0.000	565488964	0.8644	9999000000	150415151	No	0.00%				76.9%	82.7%	94.2%	278.0M	39.1%	96.9%	62576.8Mb	590.6M	56.0%	98.4%	84.1%
1173_T46_T1_RNA_SLD_01_A23T55JLT4_1_1																																				108.6bp	98.0%	289.4M												86.0%	53%	151bp	151bp	27%	314.7M
1173_T46_T1_RNA_SLD_01_A23T55JLT4_1_2																																																		83.4%	55%	151bp	151bp	27%	314.7M
1173_T46_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_1																																																		85.7%	55%	109bp	107bp	18%	295.3M	85.7%	55%	109bp	107bp	18%	295.3M
1173_T46_T1_RNA_SLD_01_A23T55JLT4_1_trimmed_2																																																		84.2%	56%	109bp	107bp	18%	295.3M	84.2%	56%	109bp	107bp	18%	295.3M
1173_T46_T1_RNA_SLD_01_A23T55JLT4_2	107.6bp	102.0bp	0.00%	0.93%	0.29%	0.00%	0.00%	2.98%	0.49%	0.00%	5.95	544744504	0.01%	0.78%	118341229	0.90%	0.95%	0.06%	0.05%	106384653	53121844	7582049	3000221	148.85	46.00	0.57%	0.35%	0.97	0.000	521607901	0.9272	9999000000	96735325	No	0.00%				78.9%	84.5%	94.3%	256.9M	48.2%	97.2%	57140.0Mb	544.7M	56.3%	97.9%	84.9%
1173_T46_T1_RNA_SLD_01_A23T55JLT4_2_1																																				107.6bp	98.3%	267.8M												87.6%	54%	151bp	151bp	27%	278.2M
1173_T46_T1_RNA_SLD_01_A23T55JLT4_2_2																																																		84.9%	55%	151bp	151bp	27%	278.2M
1173_T46_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_1																																																		87.6%	55%	108bp	107bp	18%	272.4M	87.6%	55%	108bp	107bp	18%	272.4M
1173_T46_T1_RNA_SLD_01_A23T55JLT4_2_trimmed_2																																																		86.5%	56%	108bp	107bp	18%	272.4M	86.5%	56%	108bp	107bp	18%	272.4M
FFPE_HD789_01_RNA_13_A23T55JLT4_1	177.8bp	161.0bp	0.01%	1.71%	0.76%	0.00%	0.01%	4.92%	1.09%	0.00%	4.03	588625368	0.01%	0.41%	346905145	0.95%	0.98%	0.04%	0.02%	328132783	166249033	15195598	3643984	728.18	298.00	0.72%	0.62%	0.97	0.000	569256851	0.9578	9999000000	524299752	Yes	62.38%				41.3%	90.2%	95.9%	282.3M	30.1%	97.1%	78248.3Mb	588.6M	54.0%	98.1%	38.8%
FFPE_HD789_01_RNA_13_A23T55JLT4_1_1																																				177.8bp	98.3%	289.2M												88.6%	53%	151bp	151bp	18%	604.3M
FFPE_HD789_01_RNA_13_A23T55JLT4_1_2																																																		87.2%	54%	151bp	151bp	18%	604.3M
FFPE_HD789_01_RNA_13_A23T55JLT4_1_trimmed_1																																																		84.4%	53%	137bp	151bp	9%	294.3M	84.4%	53%	137bp	151bp	9%	294.3M
FFPE_HD789_01_RNA_13_A23T55JLT4_1_trimmed_2																																																		84.9%	54%	137bp	151bp	9%	294.3M	84.9%	54%	137bp	151bp	9%	294.3M
FFPE_V4_0002_RNA_0001_A23T55JLT4_1	161.6bp	147.0bp	0.01%	1.80%	1.18%	0.00%	0.01%	4.90%	1.62%	0.00%	6.78	587982300	0.01%	0.52%	280130995	0.93%	0.97%	0.05%	0.03%	261233495	132351575	14600392	4061714	541.16	135.00	0.60%	0.52%	0.97	0.000	566780030	0.9729	9999000000	287236748	No	0.00%				52.7%	89.5%	95.6%	281.0M	37.5%	97.1%	76273.8Mb	588.0M	54.2%	98.0%	45.6%
FFPE_V4_0002_RNA_0001_A23T55JLT4_1_1																																				161.6bp	97.4%	286.4M												87.1%	53%	151bp	151bp	18%	356.2M
FFPE_V4_0002_RNA_0001_A23T55JLT4_1_2																																																		86.0%	54%	151bp	151bp	18%	356.2M
FFPE_V4_0002_RNA_0001_A23T55JLT4_1_trimmed_1																																																		86.1%	53%	134bp	151bp	9%	294.0M	86.1%	53%	134bp	151bp	9%	294.0M
FFPE_V4_0002_RNA_0001_A23T55JLT4_1_trimmed_2																																																		86.6%	54%	134bp	151bp	9%	294.0M	86.6%	54%	134bp	151bp	9%	294.0M
NTC_0001_0001_A23T55JLT4_1	91.5bp	100.0bp	0.00%	0.00%	0.00%	0.00%	0.00%	0.00%	0.00%	0.00%	0.00	1444	0.44%	0.22%	1129	0.08%	0.20%	0.35%	0.80%	91	47	398	188	0.00	0.00	0.00%	0.00%	0.94	0.000	288	0.4733	9999000000	2116	Skipped	N/A%				39.2%	4.7%	17.7%	0.0M	29.4%	89.0%	0.1Mb	0.0M	68.5%	4.1%	50.8%
NTC_0001_0001_A23T55JLT4_1_1																																				91.5bp	19.4%	0.0M												94.8%	73%	151bp	151bp	55%	0.0M
NTC_0001_0001_A23T55JLT4_1_2																																																		95.2%	98%	151bp	151bp	55%	0.0M
NTC_0001_0001_A23T55JLT4_1_trimmed_1																																																		32.1%	64%	92bp	92bp	45%	0.0M	32.1%	64%	92bp	92bp	45%	0.0M
NTC_0001_0001_A23T55JLT4_1_trimmed_2																																																		39.9%	71%	95bp	97bp	45%	0.0M	39.9%	71%	95bp	97bp	45%	0.0M

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order. Table ID: table-general_stats_table

Sort	Group	Column	Description	ID	Scale
\|\|	Custom content: Kallisto	Fragment Length Mean	Mean fragment length from kallisto fragment length distribution	`fragment_length_mean`	None
\|\|	Custom content: Kallisto	Fragment Length Median	Median fragment length from kallisto fragment length distribution	`fragment_length_median`	None
\|\|	Custom content: Expression QC	pct Mito est. counts	Percentage of estimated counts from mitochondrial genes; high values can indicate cell damage or stress.	`pct_mito_est_counts`	None
\|\|	Custom content: Expression QC	pct Ribo est. counts	Percentage of estimated counts from ribosomal genes; useful for assessing RNA quality and technical variation.	`pct_ribo_est_counts`	None
\|\|	Custom content: Expression QC	pct Housekeeping est. counts	Percentage of estimated counts from housekeeping genes.	`pct_hk_est_counts`	None
\|\|	Custom content: Expression QC	pct Male est. counts	pct Male est. counts	`pct_male_est_counts`	None
\|\|	Custom content: Expression QC	pct Mito TPM	Percentage of TPM from mitochondrial genes; high values can indicate cell damage or stress.	`pct_mito_tpm`	None
\|\|	Custom content: Expression QC	pct Ribo TPM	Percentage of TPM from ribosomal genes; useful for assessing RNA quality and technical variation.	`pct_ribo_tpm`	None
\|\|	Custom content: Expression QC	pct Housekeeping TPM	Percentage of TPM from housekeeping genes.	`pct_hk_tpm`	None
\|\|	Custom content: Expression QC	pct Male TPM	pct Male TPM	`pct_male_tpm`	None
\|\|	Custom content: Expression QC	ChrY Signature Score	Mean log2(TPM+1) score for chrY signature genes; used for sex verification.	`score_log2tpm1_mean_chry_signature`	None
\|\|	Custom content: SeqTools (general stats)	Reads	SeqTools (general stats) — reads	`reads`	None
\|\|	Custom content: SeqTools (general stats)	Frac Unmapped	SeqTools (general stats) — frac unmapped	`frac_unmapped`	None
\|\|	Custom content: SeqTools (general stats)	Frac Dupes	SeqTools (general stats) — frac dupes	`frac_dupes`	None
\|\|	Custom content: SeqTools (general stats)	Unique Reads	SeqTools (general stats) — unique reads	`unique_reads`	None
\|\|	Custom content: SeqTools (general stats)	Frac Reads On Target	SeqTools (general stats) — frac reads on target	`frac_reads_on_target`	None
\|\|	Custom content: SeqTools (general stats)	Frac Pairs On Target	SeqTools (general stats) — frac pairs on target	`frac_pairs_on_target`	None
\|\|	Custom content: SeqTools (general stats)	Frac Reads Off Target	SeqTools (general stats) — frac reads off target	`frac_reads_off_target`	None
\|\|	Custom content: SeqTools (general stats)	Frac Pairs Off Target	SeqTools (general stats) — frac pairs off target	`frac_pairs_off_target`	None
\|\|	Custom content: SeqTools (general stats)	Reads On Target	SeqTools (general stats) — reads on target	`reads_on_target`	None
\|\|	Custom content: SeqTools (general stats)	Pairs On Target	SeqTools (general stats) — pairs on target	`pairs_on_target`	None
\|\|	Custom content: SeqTools (general stats)	Reads Off Target	SeqTools (general stats) — reads off target	`reads_off_target`	None
\|\|	Custom content: SeqTools (general stats)	Pairs Off Target	SeqTools (general stats) — pairs off target	`pairs_off_target`	None
\|\|	Custom content: SeqTools (general stats)	Mean Depth	SeqTools (general stats) — mean depth	`mean_depth`	None
\|\|	Custom content: SeqTools (general stats)	Q50 Depth	SeqTools (general stats) — q50 depth	`q50_depth`	None
\|\|	Custom content: SeqTools (general stats)	Frac Bases Gt30X	SeqTools (general stats) — frac bases gt30x	`frac_bases_gt30x`	None
\|\|	Custom content: SeqTools (general stats)	Frac Bases Gt100X	SeqTools (general stats) — frac bases gt100x	`frac_bases_gt100x`	None
\|\|	Custom content: SeqTools (general stats)	N Q30	SeqTools (general stats) — n q30	`n_q30`	None
\|\|	Custom content: SeqTools (general stats)	GC Bias	SeqTools (general stats) — Ratio of high GC (63-100%) to low GC (0-43%) reads	`gc_bias`	None
\|\|	Custom content: RSeQC	Unique Reads	Reads with mapq >= mapq_cut (uniquely mapped)	`unique_reads_rseqc`	None
\|\|	Custom content: RSeQC	Frac Antisense	Fraction of reads explained by antisense orientation (1+-,1-+,2++,2--)	`frac_antisense`	None
\|\|	Custom content: Preseq	Total Reads	Total reads from preseq analysis	`total_reads`	None
\|\|	Custom content: Preseq	Expected Distinct	Expected distinct molecules at maximum sequencing depth	`expected_distinct`	None
\|\|	Custom content: RNA contamination	RNA contaminated	Whether the sample was classified as contaminated from VAF_p90	`is_contaminated`	None
\|\|	Custom content: RNA contamination	RNA contamination %	Estimated RNA contamination from VAF_p90; 0% for samples classified as clean	`contamination_pct`	None
\|\|	Kallisto	Frag Length	Estimated average fragment length	`fragment_length`	None
\|\|	Kallisto	% Aligned	% processed reads that were pseudoaligned	`percent_aligned`	None
\|\|	Kallisto	M Aligned	Pseudoaligned reads (millions)	`pseudoaligned_reads`	read_count
\|\|	Picard: Mark Duplicates	Duplication	Mark Duplicates - Percent Duplication	`PERCENT_DUPLICATION`	None
\|\|	RSeQC	% Proper Pairs	% Reads mapped in proper pairs	`proper_pairs_percent`	None
\|\|	STAR	% Aligned	% Uniquely mapped reads	`uniquely_mapped_percent`	None
\|\|	STAR	M Aligned	Uniquely mapped reads (millions)	`uniquely_mapped`	read_count
\|\|	fastp	% Duplication	Duplication rate before filtering	`pct_duplication`	None
\|\|	fastp	% > Q30	Percentage of reads > Q30 after filtering	`after_filtering_q30_rate`	None
\|\|	fastp	Mb Q30 bases	Bases > Q30 after filtering (millions)	`after_filtering_q30_bases`	base_count
\|\|	fastp	M Reads After Filtering	Total reads after filtering (millions)	`filtering_result_passed_filter_reads`	read_count
\|\|	fastp	GC content	GC content after filtering	`after_filtering_gc_content`	None
\|\|	fastp	% PF	Percent reads passing filter	`pct_surviving`	None
\|\|	fastp	% Adapter	Percentage adapter-trimmed reads	`pct_adapter`	None
\|\|	FastQC (raw)	% Dups	% Duplicate Reads	`percent_duplicates`	None
\|\|	FastQC (raw)	% GC	Average % GC Content	`percent_gc`	None
\|\|	FastQC (raw)	Average Read Length	Average Read Length (bp)	`avg_sequence_length`	None
\|\|	FastQC (raw)	Median Read Length	Median Read Length (bp)	`median_sequence_length`	None
\|\|	FastQC (raw)	% Failed	Percentage of modules failed in FastQC report (includes those not plotted here)	`percent_fails`	None
\|\|	FastQC (raw)	M Seqs	Total Sequences (millions)	`total_sequences`	read_count
\|\|	FastQC (trimmed)	% Dups	% Duplicate Reads	`percent_duplicates`	None
\|\|	FastQC (trimmed)	% GC	Average % GC Content	`percent_gc`	None
\|\|	FastQC (trimmed)	Average Read Length	Average Read Length (bp)	`avg_sequence_length`	None
\|\|	FastQC (trimmed)	Median Read Length	Median Read Length (bp)	`median_sequence_length`	None
\|\|	FastQC (trimmed)	% Failed	Percentage of modules failed in FastQC report (includes those not plotted here)	`percent_fails`	None
\|\|	FastQC (trimmed)	M Seqs	Total Sequences (millions)	`total_sequences`	read_count

Kallisto

Kallisto is a program for quantifying abundances of transcripts from RNA-Seq data.DOI: 10.1038/nbt.3519.

Created with MultiQC

Picard

Picard is a set of Java command line tools for manipulating high-throughput sequencing data.

Mark Duplicates

Number of reads, categorised by duplication state. Pair counts are doubled - see help text for details.

The table in the Picard metrics file contains some columns referring read pairs and some referring to single reads.

To make the numbers in this plot sum correctly, values referring to pairs are doubled according to the scheme below:

READS_IN_DUPLICATE_PAIRS = 2 * READ_PAIR_DUPLICATES
READS_IN_UNIQUE_PAIRS = 2 * (READ_PAIRS_EXAMINED - READ_PAIR_DUPLICATES)
READS_IN_UNIQUE_UNPAIRED = UNPAIRED_READS_EXAMINED - UNPAIRED_READ_DUPLICATES
READS_IN_DUPLICATE_PAIRS_OPTICAL = 2 * READ_PAIR_OPTICAL_DUPLICATES
READS_IN_DUPLICATE_PAIRS_NONOPTICAL = READS_IN_DUPLICATE_PAIRS - READS_IN_DUPLICATE_PAIRS_OPTICAL
READS_IN_DUPLICATE_UNPAIRED = UNPAIRED_READ_DUPLICATES
READS_UNMAPPED = UNMAPPED_READS

Created with MultiQC

Preseq

Preseq estimates the complexity of a library, showing how many additional unique reads are sequenced for increasing total read count. A shallow curve indicates complexity saturation. The dashed line shows a perfectly complex library where total reads = unique reads.DOI: 10.1038/nmeth.2375.

Complexity curve (molecule count)

Note that the x-axis is trimmed at the point where all the datasets show 80% of their maximum y-value, to avoid ridiculous scales.

Created with MultiQC

RSeQC

RSeQC package provides a number of useful modules that can comprehensively evaluate high throughput RNA-seq data.DOI: 10.1093/bioinformatics/bts356.

Read Distribution

Read Distribution calculates how mapped reads are distributed over genome features.

Created with MultiQC

Inner Distance

Inner Distance calculates the inner distance (or insert size) between two paired RNA reads. Note that this can be negative if fragments overlap.

Created with MultiQC

Read Duplication

read_duplication.py calculates how many alignment positions have a certain number of exact duplicates. Note - plot truncated at 500 occurrences and binned.

Created with MultiQC

Junction Annotation

Junction annotation compares detected splice junctions to a reference gene model. An RNA read can be spliced 2 or more times, each time is called a splicing event.

Created with MultiQC

Junction Saturation

Junction Saturation counts the number of known splicing junctions that are observed in each dataset. If sequencing depth is sufficient, all (annotated) splice junctions should be rediscovered, resulting in a curve that reaches a plateau. Missing low abundance splice junctions can affect downstream analysis.

Click a line to see the data side by side (as in the original RSeQC plot).

Created with MultiQC

Infer experiment

Infer experiment counts the percentage of reads and read pairs that match the strandedness of overlapping transcripts. It can be used to infer whether RNA-seq library preps are stranded (sense or antisense).

Created with MultiQC

Bam Stat

All numbers reported in millions.

Created with MultiQC

Showing ⁹⁶/₉₆ rows and ¹⁴/₁₄ columns.

Sample Name	Total records	QC failed	Duplicates	Non primary hit	Unmapped	Unique	Read-1	Read-2	+ve strand	-ve strand	Non-splice reads	Splice reads	Proper pairs	Different chrom
1173_9C_T1_RNA_SLD_01_A23T55JLT4_1	659.6M	0.0M	0.0M	68.1M	4.5M	560.7M	280.5M	280.1M	280.4M	280.3M	349.4M	211.3M	558.3M	0.0M
1173_BEE_T1_RNA_SLD_01_A23T55JLT4_1	639.4M	0.0M	0.0M	71.6M	4.5M	533.1M	266.7M	266.4M	266.6M	266.5M	341.0M	192.1M	531.0M	0.0M
1173_C31_T1_RNA_SLD_01_A23T55JLT4_1	228.1M	0.0M	0.0M	21.7M	1.5M	195.7M	97.9M	97.8M	97.9M	97.8M	117.5M	78.2M	195.0M	0.0M
1173_C31_T1_RNA_SLD_01_A23T55JLT4_2	339.7M	0.0M	0.0M	32.6M	2.5M	290.2M	145.2M	145.0M	145.1M	145.1M	173.7M	116.5M	289.1M	0.0M
1173_CH0_T1_RNA_SLD_01_A23T55JLT4_1	74.3M	0.0M	0.0M	7.5M	0.5M	63.4M	31.8M	31.7M	31.7M	31.7M	42.8M	20.7M	63.2M	0.0M
1173_CH0_T1_RNA_SLD_01_A23T55JLT4_2	388.4M	0.0M	0.0M	41.1M	2.7M	329.0M	164.7M	164.3M	164.6M	164.5M	222.3M	106.7M	327.7M	0.0M
1173_CI9_T1_RNA_SLD_01_A23T55JLT4_1	544.1M	0.0M	0.0M	53.4M	3.9M	466.4M	233.3M	233.1M	233.2M	233.1M	310.8M	155.6M	465.1M	0.0M
1173_CRL_T1_RNA_SLD_01_A23T55JLT4_1	646.3M	0.0M	0.0M	52.6M	4.0M	566.9M	283.5M	283.4M	283.5M	283.4M	333.3M	233.6M	564.6M	0.0M
1173_CRL_T1_RNA_SLD_01_A23T55JLT4_2	644.4M	0.0M	0.0M	52.2M	4.5M	565.1M	282.7M	282.4M	282.6M	282.5M	323.0M	242.2M	562.7M	0.0M
1173_CRX_T1_RNA_SLD_01_A23T55JLT4_1	25.3M	0.0M	0.0M	2.5M	0.2M	21.7M	10.9M	10.9M	10.9M	10.9M	13.4M	8.3M	21.6M	0.0M
1173_CRX_T1_RNA_SLD_01_A23T55JLT4_2	652.1M	0.0M	0.0M	62.0M	4.5M	561.6M	281.1M	280.5M	280.9M	280.7M	333.0M	228.7M	559.3M	0.0M
1173_D6K_T1_RNA_SLD_01_A23T55JLT4_1	635.4M	0.0M	0.0M	41.6M	3.6M	572.8M	286.5M	286.3M	286.5M	286.3M	300.4M	272.4M	570.2M	0.0M
1173_D6K_T1_RNA_SLD_01_A23T55JLT4_2	630.8M	0.0M	0.0M	38.4M	3.9M	571.7M	286.0M	285.7M	285.9M	285.8M	287.4M	284.3M	569.0M	0.0M
1173_DSO_T1_RNA_SLD_01_A23T55JLT4_1	696.1M	0.0M	0.0M	103.9M	4.0M	545.1M	272.7M	272.5M	272.6M	272.5M	343.8M	201.3M	543.4M	0.0M
1173_DSO_T1_RNA_SLD_01_A23T55JLT4_2	484.8M	0.0M	0.0M	70.1M	3.2M	381.6M	191.0M	190.7M	190.9M	190.8M	226.9M	154.7M	380.0M	0.0M
1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_1	688.1M	0.0M	0.0M	95.5M	3.9M	549.9M	275.1M	274.8M	275.0M	274.9M	338.1M	211.8M	548.3M	0.0M
1173_F0Y_T1_RNA_SLD_01_A23T55JLT4_2	679.7M	0.0M	0.0M	103.6M	4.7M	531.3M	265.9M	265.3M	265.7M	265.6M	319.9M	211.3M	529.2M	0.0M
1173_FM_T1_RNA_SLD_01_A23T55JLT4_1	666.7M	0.0M	0.0M	76.6M	4.7M	558.6M	279.5M	279.1M	279.4M	279.2M	350.5M	208.1M	556.7M	0.0M
1173_FM_T1_RNA_SLD_01_A23T55JLT4_2	667.5M	0.0M	0.0M	78.2M	5.5M	556.6M	278.6M	278.0M	278.4M	278.2M	332.3M	224.4M	554.0M	0.0M
1173_FWA_T1_RNA_SLD_01_A23T55JLT4_1	657.7M	0.0M	0.0M	67.0M	4.6M	558.2M	279.3M	278.9M	279.2M	279.0M	322.1M	236.1M	556.2M	0.0M
1173_FWA_T1_RNA_SLD_01_A23T55JLT4_2	0.1M	0.0M	0.0M	0.1M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M
1173_G2E_T1_RNA_SLD_01_A23T55JLT4_1	680.7M	0.0M	0.0M	90.4M	5.5M	552.1M	276.4M	275.7M	276.1M	276.0M	381.6M	170.5M	550.2M	0.0M
1173_G2E_T1_RNA_SLD_01_A23T55JLT4_2	655.3M	0.0M	0.0M	88.8M	5.7M	528.2M	264.5M	263.8M	264.1M	264.1M	357.5M	170.7M	526.3M	0.0M
1173_GFO_T1_RNA_SLD_01_A23T55JLT4_1	659.2M	0.0M	0.0M	75.4M	7.4M	545.5M	273.0M	272.5M	272.8M	272.7M	357.2M	188.3M	543.3M	0.0M
1173_GFO_T1_RNA_SLD_01_A23T55JLT4_2	549.6M	0.0M	0.0M	63.6M	6.9M	452.8M	226.7M	226.1M	226.5M	226.3M	297.3M	155.5M	450.8M	0.0M
1173_GXL_T1_RNA_SLD_01_A23T55JLT4_1	651.6M	0.0M	0.0M	59.5M	4.1M	564.9M	282.6M	282.3M	282.5M	282.4M	375.8M	189.1M	563.0M	0.0M
1173_GXL_T1_RNA_SLD_01_A23T55JLT4_2	628.8M	0.0M	0.0M	60.6M	4.4M	540.9M	270.7M	270.2M	270.5M	270.4M	348.4M	192.5M	538.8M	0.0M
1173_GZ1_T1_RNA_SLD_01_A23T55JLT4_1	34.2M	0.0M	0.0M	3.2M	0.2M	29.4M	14.7M	14.7M	14.7M	14.7M	18.0M	11.4M	29.3M	0.0M
1173_GZ1_T1_RNA_SLD_01_A23T55JLT4_2	623.2M	0.0M	0.0M	60.1M	5.2M	533.4M	266.9M	266.5M	266.8M	266.6M	318.5M	214.9M	531.5M	0.0M
1173_H2E_T1_RNA_SLD_01_A23T55JLT4_1	646.5M	0.0M	0.0M	55.4M	4.6M	564.1M	282.1M	282.0M	282.1M	282.0M	341.9M	222.3M	562.2M	0.0M
1173_H2E_T1_RNA_SLD_01_A23T55JLT4_2	641.6M	0.0M	0.0M	50.8M	4.9M	564.6M	282.4M	282.2M	282.4M	282.2M	322.5M	242.1M	562.4M	0.0M
1173_HOB_T1_RNA_SLD_01_A23T55JLT4_1	668.5M	0.0M	0.0M	76.3M	4.2M	558.5M	279.4M	279.1M	279.3M	279.2M	337.2M	221.3M	556.7M	0.0M
1173_HOB_T1_RNA_SLD_01_A23T55JLT4_2	543.2M	0.0M	0.0M	65.1M	3.7M	449.6M	225.0M	224.6M	224.9M	224.7M	267.6M	182.0M	447.8M	0.0M
1173_HRO_T1_RNA_SLD_01_A23T55JLT4_1	664.7M	0.0M	0.0M	73.3M	4.2M	555.9M	278.1M	277.8M	278.0M	277.9M	345.9M	210.1M	554.3M	0.0M
1173_IT6_T1_RNA_SLD_01_A23T55JLT4_1	600.2M	0.0M	0.0M	75.9M	3.5M	497.5M	248.8M	248.7M	248.8M	248.7M	322.4M	175.2M	496.5M	0.0M
1173_IT6_T1_RNA_SLD_01_A23T55JLT4_2	336.3M	0.0M	0.0M	34.7M	2.2M	287.2M	143.7M	143.5M	143.6M	143.6M	182.0M	105.3M	286.3M	0.0M
1173_J3R_T1_RNA_SLD_01_A23T55JLT4_1	553.1M	0.0M	0.0M	78.6M	3.3M	444.3M	222.2M	222.0M	222.2M	222.1M	316.1M	128.1M	443.0M	0.0M
1173_J3R_T1_RNA_SLD_01_A23T55JLT4_2	412.2M	0.0M	0.0M	60.4M	2.8M	328.4M	164.4M	164.0M	164.2M	164.2M	223.8M	104.6M	327.0M	0.0M
1173_JIG_T1_RNA_SLD_01_A23T55JLT4_1	187.0M	0.0M	0.0M	130.8M	1.5M	35.2M	17.6M	17.6M	17.6M	17.6M	24.4M	10.8M	35.0M	0.0M
1173_JLC_T1_RNA_SLD_01_A23T55JLT4_1	648.3M	0.0M	0.0M	55.7M	4.5M	565.2M	282.7M	282.5M	282.7M	282.5M	323.1M	242.1M	562.9M	0.0M
1173_JLC_T1_RNA_SLD_01_A23T55JLT4_2	646.7M	0.0M	0.0M	54.8M	4.9M	564.3M	282.3M	282.0M	282.2M	282.1M	310.1M	254.2M	561.6M	0.0M
1173_JXL_T1_RNA_SLD_01_A23T55JLT4_1	618.1M	0.0M	0.0M	82.6M	3.6M	507.9M	254.0M	253.9M	254.0M	253.9M	281.5M	226.4M	505.9M	0.0M
1173_JXL_T1_RNA_SLD_01_A23T55JLT4_2	646.5M	0.0M	0.0M	53.7M	3.8M	568.1M	284.1M	283.9M	284.1M	284.0M	306.2M	261.9M	565.8M	0.0M
1173_K3O_T1_RNA_SLD_01_A23T55JLT4_1	32.0M	0.0M	0.0M	3.5M	0.2M	27.0M	13.5M	13.5M	13.5M	13.5M	19.7M	7.2M	26.9M	0.0M
1173_K3O_T1_RNA_SLD_01_A23T55JLT4_2	334.5M	0.0M	0.0M	38.6M	2.9M	278.8M	139.6M	139.2M	139.4M	139.4M	199.3M	79.5M	277.6M	0.0M
1173_K7E_T1_RNA_SLD_01_A23T55JLT4_1	658.9M	0.0M	0.0M	65.8M	3.5M	563.2M	281.7M	281.5M	281.6M	281.5M	372.2M	191.0M	561.6M	0.0M
1173_K7E_T1_RNA_SLD_01_A23T55JLT4_2	482.1M	0.0M	0.0M	50.0M	3.0M	409.4M	204.8M	204.6M	204.8M	204.7M	268.0M	141.5M	408.0M	0.0M
1173_KQG_T1_RNA_SLD_01_A23T55JLT4_1	319.9M	0.0M	0.0M	25.2M	1.6M	282.9M	141.5M	141.4M	141.5M	141.4M	163.9M	119.1M	281.9M	0.0M
1173_KQG_T1_RNA_SLD_01_A23T55JLT4_2	641.5M	0.0M	0.0M	47.5M	3.7M	570.6M	285.5M	285.1M	285.4M	285.2M	315.4M	255.2M	568.2M	0.0M
1173_KRT_T1_RNA_SLD_01_A23T55JLT4_1	26.6M	0.0M	0.0M	5.9M	0.2M	18.8M	9.4M	9.4M	9.4M	9.4M	13.4M	5.4M	18.7M	0.0M
1173_KTN_T1_RNA_SLD_01_A23T55JLT4_1	652.5M	0.0M	0.0M	58.3M	4.3M	565.9M	283.0M	282.9M	283.0M	282.9M	365.7M	200.3M	564.6M	0.0M
1173_KTN_T1_RNA_SLD_01_A23T55JLT4_2	488.2M	0.0M	0.0M	44.1M	3.6M	422.6M	211.5M	211.2M	211.4M	211.3M	264.3M	158.3M	421.1M	0.0M
1173_LAF_T1_RNA_SLD_01_A23T55JLT4_1	219.0M	0.0M	0.0M	126.1M	1.4M	69.7M	34.9M	34.8M	34.8M	34.8M	51.1M	18.6M	69.5M	0.0M
1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2	309.8M	0.0M	0.0M	148.5M	2.1M	131.1M	65.6M	65.5M	65.5M	65.5M	93.4M	37.7M	130.7M	0.0M
1173_LQD_T1_RNA_SLD_01_A23T55JLT4_1	651.0M	0.0M	0.0M	58.1M	4.6M	566.3M	283.3M	283.1M	283.2M	283.1M	328.8M	237.5M	564.1M	0.0M
1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1	650.4M	0.0M	0.0M	61.8M	4.5M	560.8M	280.6M	280.2M	280.4M	280.3M	368.0M	192.8M	558.3M	0.0M
1173_LV7_T1_RNA_SLD_01_A23T55JLT4_2	648.2M	0.0M	0.0M	58.8M	4.5M	562.4M	281.5M	280.9M	281.3M	281.1M	358.1M	204.3M	560.1M	0.0M
1173_M2F_T1_RNA_SLD_01_A23T55JLT4_1	516.4M	0.0M	0.0M	47.7M	2.8M	446.9M	223.5M	223.4M	223.5M	223.4M	297.1M	149.8M	445.9M	0.0M
1173_M4Y_T1_RNA_SLD_01_A23T55JLT4_1	365.3M	0.0M	0.0M	67.5M	3.1M	274.9M	137.7M	137.2M	137.4M	137.4M	205.5M	69.4M	273.5M	0.0M
1173_M5U_T1_RNA_SLD_01_A23T55JLT4_1	660.9M	0.0M	0.0M	68.5M	4.1M	560.6M	280.5M	280.1M	280.4M	280.2M	343.8M	216.8M	558.6M	0.0M
1173_M5U_T1_RNA_SLD_01_A23T55JLT4_2	646.2M	0.0M	0.0M	70.9M	4.3M	542.7M	271.6M	271.1M	271.4M	271.3M	325.7M	216.9M	540.4M	0.0M
1173_MHK_T1_RNA_SLD_01_A23T55JLT4_1	669.5M	0.0M	0.0M	77.9M	5.3M	556.7M	278.6M	278.1M	278.4M	278.3M	346.0M	210.7M	554.5M	0.0M
1173_N5V_T1_RNA_SLD_01_A23T55JLT4_1	443.3M	0.0M	0.0M	39.9M	2.1M	383.9M	192.0M	191.9M	192.0M	191.9M	261.2M	122.7M	382.8M	0.0M
1173_NBF_T1_RNA_SLD_01_A23T55JLT4_1	524.1M	0.0M	0.0M	50.9M	3.8M	449.1M	224.6M	224.5M	224.6M	224.5M	284.6M	164.5M	447.9M	0.0M
1173_NFO_T1_RNA_SLD_01_A23T55JLT4_1	672.8M	0.0M	0.0M	80.7M	5.3M	559.2M	280.0M	279.3M	279.7M	279.6M	396.8M	162.4M	557.0M	0.0M
1173_NFO_T1_RNA_SLD_01_A23T55JLT4_2	502.9M	0.0M	0.0M	66.1M	4.3M	410.7M	205.7M	205.0M	205.4M	205.3M	286.5M	124.2M	408.9M	0.0M
1173_NMN_T1_RNA_SLD_01_A23T55JLT4_1	607.6M	0.0M	0.0M	69.1M	4.4M	508.1M	254.3M	253.9M	254.1M	254.0M	333.6M	174.5M	506.0M	0.0M
1173_NMN_T1_RNA_SLD_01_A23T55JLT4_2	582.8M	0.0M	0.0M	64.8M	4.5M	488.5M	244.5M	244.0M	244.3M	244.2M	316.5M	172.0M	486.5M	0.0M
1173_NWE_T1_RNA_SLD_01_A23T55JLT4_1	640.6M	0.0M	0.0M	46.3M	3.4M	571.8M	285.9M	285.8M	285.9M	285.8M	318.2M	253.6M	569.9M	0.0M
1173_NWE_T1_RNA_SLD_01_A23T55JLT4_2	634.9M	0.0M	0.0M	41.6M	3.6M	571.5M	285.8M	285.6M	285.8M	285.7M	304.5M	266.9M	569.3M	0.0M
1173_OHF_T1_RNA_SLD_01_A23T55JLT4_1	10.1M	0.0M	0.0M	1.3M	0.1M	8.2M	4.1M	4.1M	4.1M	4.1M	6.3M	1.9M	8.2M	0.0M
1173_PCB_T1_RNA_SLD_01_A23T55JLT4_1	231.8M	0.0M	0.0M	20.7M	1.5M	201.1M	100.6M	100.5M	100.6M	100.5M	135.0M	66.1M	200.4M	0.0M
1173_PCB_T1_RNA_SLD_01_A23T55JLT4_2	510.3M	0.0M	0.0M	46.1M	3.7M	441.5M	221.0M	220.6M	220.8M	220.7M	287.3M	154.2M	439.7M	0.0M
1173_Q7O_T1_RNA_SLD_01_A23T55JLT4_1	648.3M	0.0M	0.0M	56.2M	4.5M	565.7M	283.0M	282.7M	282.9M	282.8M	357.8M	207.9M	563.1M	0.0M
1173_Q7O_T1_RNA_SLD_01_A23T55JLT4_2	543.4M	0.0M	0.0M	47.0M	4.1M	473.7M	237.0M	236.6M	236.9M	236.8M	290.0M	183.7M	471.1M	0.0M
1173_QEV_T1_RNA_SLD_01_A23T55JLT4_1	23.8M	0.0M	0.0M	4.0M	0.2M	18.2M	9.1M	9.1M	9.1M	9.1M	12.6M	5.6M	18.1M	0.0M
1173_QJL_T1_RNA_SLD_01_A23T55JLT4_1	346.6M	0.0M	0.0M	35.5M	2.0M	295.6M	147.9M	147.8M	147.8M	147.8M	189.0M	106.7M	294.8M	0.0M
1173_QJL_T1_RNA_SLD_01_A23T55JLT4_2	538.5M	0.0M	0.0M	57.8M	3.5M	456.0M	228.1M	227.9M	228.1M	228.0M	285.0M	171.0M	454.3M	0.0M
1173_RDM_T1_RNA_SLD_01_A23T55JLT4_1	632.1M	0.0M	0.0M	76.3M	5.9M	523.1M	262.0M	261.1M	261.6M	261.5M	356.3M	166.8M	520.4M	0.0M
1173_RDM_T1_RNA_SLD_01_A23T55JLT4_2	318.8M	0.0M	0.0M	42.4M	3.2M	258.4M	129.5M	129.0M	129.3M	129.2M	181.2M	77.3M	257.3M	0.0M
1173_RGW_T1_RNA_SLD_01_A23T55JLT4_1	286.9M	0.0M	0.0M	69.6M	3.4M	193.9M	97.2M	96.7M	97.0M	96.9M	147.0M	46.9M	192.8M	0.0M
1173_RPC_T1_RNA_SLD_01_A23T55JLT4_1	60.5M	0.0M	0.0M	5.4M	0.4M	52.6M	26.3M	26.3M	26.3M	26.3M	33.5M	19.1M	52.5M	0.0M
1173_RPC_T1_RNA_SLD_01_A23T55JLT4_2	615.0M	0.0M	0.0M	53.1M	4.0M	536.5M	268.4M	268.1M	268.3M	268.2M	335.1M	201.4M	534.8M	0.0M
1173_RPX_T1_RNA_SLD_01_A23T55JLT4_1	659.4M	0.0M	0.0M	66.8M	4.2M	562.8M	281.6M	281.2M	281.4M	281.3M	339.9M	222.9M	560.4M	0.0M
1173_RQT_T1_RNA_SLD_01_A23T55JLT4_1	608.3M	0.0M	0.0M	69.9M	4.5M	510.0M	255.4M	254.6M	255.1M	254.9M	327.5M	182.5M	507.6M	0.0M
1173_RQT_T1_RNA_SLD_01_A23T55JLT4_2	432.3M	0.0M	0.0M	49.0M	3.3M	363.3M	181.9M	181.4M	181.7M	181.6M	228.1M	135.2M	361.7M	0.0M
1173_S9Q_T1_RNA_SLD_01_A23T55JLT4_1	662.0M	0.0M	0.0M	69.6M	5.0M	561.2M	280.9M	280.3M	280.7M	280.5M	355.7M	205.5M	558.9M	0.0M
1173_SDP_T1_RNA_SLD_01_A23T55JLT4_1	651.9M	0.0M	0.0M	58.3M	4.1M	567.8M	284.1M	283.7M	284.0M	283.8M	365.9M	202.0M	566.2M	0.0M
1173_SFA_T1_RNA_SLD_01_A23T55JLT4_1	82.0M	0.0M	0.0M	12.4M	0.6M	64.4M	32.2M	32.2M	32.2M	32.2M	40.4M	24.0M	64.2M	0.0M
1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1	668.3M	0.0M	0.0M	75.4M	4.6M	561.5M	280.8M	280.7M	280.8M	280.7M	339.4M	222.1M	559.7M	0.0M
1173_SK8_T1_RNA_SLD_01_A23T55JLT4_2	451.3M	0.0M	0.0M	40.9M	3.5M	390.1M	195.2M	194.9M	195.1M	195.0M	224.1M	166.0M	388.5M	0.0M
1173_T46_T1_RNA_SLD_01_A23T55JLT4_1	679.7M	0.0M	0.0M	84.2M	4.5M	565.5M	282.5M	283.0M	282.8M	282.7M	346.1M	219.4M	562.2M	0.0M
1173_T46_T1_RNA_SLD_01_A23T55JLT4_2	614.0M	0.0M	0.0M	65.5M	4.4M	521.6M	260.7M	260.9M	260.9M	260.7M	315.3M	206.3M	518.8M	0.0M
FFPE_HD789_01_RNA_13_A23T55JLT4_1	629.0M	0.0M	0.0M	38.5M	3.6M	569.3M	284.7M	284.5M	284.7M	284.6M	256.0M	313.2M	567.4M	0.0M
FFPE_V4_0002_RNA_0001_A23T55JLT4_1	631.3M	0.0M	0.0M	41.3M	4.3M	566.8M	283.5M	283.3M	283.5M	283.3M	275.8M	291.0M	564.9M	0.0M
NTC_0001_0001_A23T55JLT4_1	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order. Table ID: table-rseqc_bam_stat

Sort	Column	Description	ID	Scale
\|\|	Total records	Total records	`total_records`	read_count
\|\|	QC failed	QC failed	`qc_failed`	read_count
\|\|	Duplicates	Optical/PCR duplicate	`optical_pcr_duplicate`	read_count
\|\|	Non primary hit	Non primary hit	`non_primary_hits`	read_count
\|\|	Unmapped	Unmapped reads	`unmapped_reads`	read_count
\|\|	Unique	mapq >= mapq_cut (unique)	`mapq_gte_mapq_cut_unique`	read_count
\|\|	Read-1	Read-1	`read_1`	read_count
\|\|	Read-2	Read-2	`read_2`	read_count
\|\|	+ve strand	Reads map to '+'	`reads_map_to_sense`	read_count
\|\|	-ve strand	Reads map to '-'	`reads_map_to_antisense`	read_count
\|\|	Non-splice reads	Non-splice reads	`non-splice_reads`	read_count
\|\|	Splice reads	Splice reads	`splice_reads`	read_count
\|\|	Proper pairs	Reads mapped in proper pairs	`reads_mapped_in_proper_pairs`	read_count
\|\|	Different chrom	Proper-paired reads map to different chrom	`proper-paired_reads_map_to_different_chrom`	read_count

STAR

STAR is an ultrafast universal RNA-seq aligner.DOI: 10.1093/bioinformatics/bts635.

Alignment Scores

Created with MultiQC

Gene Counts

Statistics from results generated using --quantMode GeneCounts. The three tabs show counts for unstranded RNA-seq, counts for the 1st read strand aligned with RNA and counts for the 2nd read strand aligned with RNA.

Created with MultiQC

fastp

fastp An ultra-fast all-in-one FASTQ preprocessor (QC, adapters, trimming, filtering, splitting...).DOI: 10.1093/bioinformatics/bty560.

Filtered Reads

Filtering statistics of sampled reads.

Created with MultiQC

Insert Sizes

Insert size estimation of sampled reads.

Created with MultiQC

Sequence Quality

Average sequencing quality over each base of all reads.

Created with MultiQC

GC Content

Average GC content over each base of all reads.

Created with MultiQC

N content

Average N content over each base of all reads.

Created with MultiQC

FastQC (raw)

FastQC (raw) This section of the report shows FastQC results before adapter trimming.

Sequence Counts

Sequence counts for each sample. Duplicate read counts are an estimate only.

This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

Created with MultiQC

Sequence Quality Histograms

The mean quality value across each base position in the read.

To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

Taken from the FastQC help:

The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

Created with MultiQC

Per Sequence Quality Scores

The number of reads with average quality scores. Shows if a subset of reads has poor quality.

From the FastQC help:

The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

Created with MultiQC

Per Base Sequence Content

The proportion of each base position for which each of the four normal DNA bases has been called.

To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

To see the data as a line plot, as in the original FastQC graph, click on a sample track.

From the FastQC help:

Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

Click a sample row to see a line plot for that dataset.

Rollover for sample name

Position: -

%T: -

%C: -

%A: -

%G: -

Per Sequence GC Content

The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

From the FastQC help:

This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

Created with MultiQC

Per Base N Content

The percentage of base calls at each position for which an N was called.

From the FastQC help:

If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

Created with MultiQC

Sequence Length Distribution

The distribution of fragment sizes (read lengths) found. See the FastQC help

Created with MultiQC

Sequence Duplication Levels

The relative level of duplication found for every sequence.

From the FastQC Help:

In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

Created with MultiQC

Overrepresented sequences by sample

The total amount of overrepresented sequences found in each library.

FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as overrepresented.

Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

From the FastQC Help:

A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

FastQC lists all the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

Created with MultiQC

Top overrepresented sequences

Top overrepresented sequences across all samples. The table shows 20 most overrepresented sequences across all samples, ranked by the number of samples they occur in.

Showing ²⁰/₂₀ rows and ³/₃ columns.

Overrepresented sequence	Samples	Occurrences	% of all reads
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	192	376710711	0.3836%
GTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCCGCCGCCGCCGCC	12	1717551	0.0017%
GCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAA	11	802377	0.0008%
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA	7	826	0.0000%
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC	6	594	0.0000%
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG	5	166	0.0000%
TTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCCGCCGCCGCCGCCGC	4	317527	0.0003%
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC	4	73	0.0000%
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC	4	70	0.0000%
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGC	4	248140	0.0003%
GCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGCCGC	4	237687	0.0002%
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC	3	185	0.0000%
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC	3	183	0.0000%
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC	3	35	0.0000%
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC	3	47	0.0000%
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC	3	46	0.0000%
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG	3	57	0.0000%
CTGCCGGGGGGGCTGTAACACTCGGGGGGCC	3	28	0.0000%
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAG	3	28	0.0000%
GTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGT	3	43	0.0000%

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order. Table ID: table-fastqc_top_overrepresented_sequences_table

Sort	Group	Column	Description	ID	Scale
\|\|	FastQC (raw)	Samples	Number of samples where this sequence is overrepresented	`samples`	None
\|\|	FastQC (raw)	Occurrences	Total number of occurrences of the sequence (among the samples where the sequence is overrepresented)	`total_count`	None
\|\|	FastQC (raw)	% of all reads	Total number of occurrences as the percentage of all reads (among samples where the sequence is overrepresented)	`total_percent`	None

Adapter Content

The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

Note that only samples with ≥ 0.1% adapter contamination are shown.

There may be several lines per sample, as one is shown for each adapter detected in the file.

From the FastQC Help:

The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

Created with MultiQC

Status Checks

Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

Min:

Max:

Created with MultiQC

FastQC (trimmed)

FastQC (trimmed) This section of the report shows FastQC results after adapter trimming.

Sequence Counts

Sequence counts for each sample. Duplicate read counts are an estimate only.

This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

Created with MultiQC

Sequence Quality Histograms

The mean quality value across each base position in the read.

To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

Taken from the FastQC help:

Created with MultiQC

Per Sequence Quality Scores

The number of reads with average quality scores. Shows if a subset of reads has poor quality.

From the FastQC help:

Created with MultiQC

Per Base Sequence Content

The proportion of each base position for which each of the four normal DNA bases has been called.

To see the data as a line plot, as in the original FastQC graph, click on a sample track.

From the FastQC help:

Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

Click a sample row to see a line plot for that dataset.

Rollover for sample name

Position: -

%T: -

%C: -

%A: -

%G: -

Per Sequence GC Content

The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

From the FastQC help:

This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

Created with MultiQC

Per Base N Content

The percentage of base calls at each position for which an N was called.

From the FastQC help:

Created with MultiQC

Sequence Length Distribution

The distribution of fragment sizes (read lengths) found. See the FastQC help

Created with MultiQC

Sequence Duplication Levels

The relative level of duplication found for every sequence.

From the FastQC Help:

The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

Created with MultiQC

Overrepresented sequences by sample

The total amount of overrepresented sequences found in each library.

From the FastQC Help:

Created with MultiQC

Top overrepresented sequences

Top overrepresented sequences across all samples. The table shows 20 most overrepresented sequences across all samples, ranked by the number of samples they occur in.

Showing ²⁰/₂₀ rows and ³/₃ columns.

Overrepresented sequence	Samples	Occurrences	% of all reads
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	96	159045941	0.3743%
GTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCCGCCGCCGCCGCC	6	853142	0.0020%
GCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAA	5	350874	0.0008%
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC	4	352	0.0000%
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA	4	417	0.0000%
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG	4	148	0.0000%
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC	4	73	0.0000%
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC	4	70	0.0000%
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC	3	185	0.0000%
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC	3	183	0.0000%
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC	3	35	0.0000%
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCC	3	47	0.0000%
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC	3	46	0.0000%
GGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGG	3	57	0.0000%
CTGCCGGGGGGGCTGTAACACTCGGGGGGCC	3	28	0.0000%
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAG	3	28	0.0000%
GTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGTGGT	3	43	0.0000%
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCA	3	61	0.0000%
CCGAGTGTTACAGCCCCCCCGGCAGCAGCA	3	94	0.0000%
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG	3	37	0.0000%

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order. Table ID: table-fastqc_top_overrepresented_sequences_table

Sort	Group	Column	Description	ID	Scale
\|\|	FastQC (trimmed)	Samples	Number of samples where this sequence is overrepresented	`samples`	None
\|\|	FastQC (trimmed)	Occurrences	Total number of occurrences of the sequence (among the samples where the sequence is overrepresented)	`total_count`	None
\|\|	FastQC (trimmed)	% of all reads	Total number of occurrences as the percentage of all reads (among samples where the sequence is overrepresented)	`total_percent`	None

Adapter Content

The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

Note that only samples with ≥ 0.1% adapter contamination are shown.

There may be several lines per sample, as one is shown for each adapter detected in the file.

From the FastQC Help:

Created with MultiQC

Status Checks

Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

Min:

Max:

Created with MultiQC

Software Versions

Software Versions lists versions of software tools extracted from file contents.

Group	Software	Version
AGAT_CONVERTSPGFF2TSV	agat	`1.2.0`
ARRIBA	arriba	`2.4.0`
ARRIBA_FILTER	filter_arriba_main.py	`1.0`
	pandas	`3.0.0`
	pyyaml	`6.0.3`
ARRIBA_VISUALISATION	arriba	`2.4.0`
CANCER_INTRONS_TO_VCF	python	`3.11.0`
CONTAMINATION_SKIPPED_LOW_READS	contamination_skipped_low_reads	`1.0.0`
CTATSPLICING_STARTOCANCERINTRONS	ctat-splicing	`0.0.3`
EXTRACT_VAF_FEATURES	extract_vaf_features	`1.0.0`
	python	`3.11.14`
FASTQC	fastqc	`0.12.1`
FASTQC_FOR_FASTP	fastqc	`0.12.1`
FUSIONINSPECTOR	STAR-Fusion	`1.12.0`
FUSIONREPORT	fusion_report	`2.1.5`
	fusion_report DB retrieval	`null`
GATK4_MARKDUPLICATES	gatk4	`4.4.0.0`
	samtools	`1.17`
GTF2BED	perl	`5.26.2`
KALLISTO_QUANT	kallisto	`0.51.1`
KALLISTO_TO_TX2GENE_QC	expression_qc_and_signatures	`1.0.0`
	python	`3.11.0`
NGSCheckMATE	ngscheckmate	`1.0.0`
PREDICT_CONTAMINATION	predict_contamination	`1.0.0`
	python	`3.11.14`
PRESEQ_LCEXTRAP	preseq	`3.2.0`
PRESEQ_TO_MULTIQC	preseq_to_multiqc	`1.0.0`
	python	`3.11.14`
RSEQC_BAMSTAT	rseqc	`5.0.2`
RSEQC_INFEREXPERIMENT	rseqc	`5.0.2`
RSEQC_INNERDISTANCE	rseqc	`5.0.2`
RSEQC_JUNCTIONANNOTATION	rseqc	`5.0.2`
RSEQC_JUNCTIONSATURATION	rseqc	`5.0.2`
RSEQC_READDISTRIBUTION	rseqc	`5.0.2`
RSEQC_READDUPLICATION	rseqc	`5.0.2`
RSEQC_TO_MULTIQC	python	`3.11.14`
	rseqc_to_multiqc	`1.0.0`
SAMTOOLS_INDEX_ALIGNMENT_CRAM	samtools	`1.17`
SAMTOOLS_INDEX_STAR_ALIGNMENT	samtools	`1.17`
SAMTOOLS_VIEW_ALIGNMENT_CRAM	samtools	`1.17`
SEQTOOL_TO_MULTIQC	python	`3.11.14`
	seqtool_to_multiqc	`1.0.0`
STARFUSION	STAR-Fusion	`1.12.0`
STARFUSION_FILTER	boto3	`unknown`
	filter_starfusion_main.py	`1.0`
	numpy	`2.4.1`
	pandas	`3.0.0`
	pyyaml	`6.0.3`
STAR_ALIGNMENT	gawk	`5.1.0`
	samtools	`1.16.1`
	star	`2.7.10a0`
VCF_COLLECT	HGNC DB retrieval	`2025-01-22/23:32`
	python	`3.11.14`
Workflow	Nextflow	`25.10.2`
	nf-core/rnafusion	`3.0.2`
fastp	fastp	`0.23.4`

nf-core/rnafusion Methods Description

Suggested text and references to use when describing pipeline usage within the methods section of a publication.

Methods

Data was processed using nf-core/rnafusion v3.0.2 of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

The pipeline was executed with Nextflow v25.10.2 (Di Tommaso et al., 2017) with the following command:

nextflow run /code/main.nf -output-dir 's3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/dev_rna_exp0016/A23T55JLT4/nfcore_rnafusion__73d51683--20260607-225254' --project-dir /Users/anaik/Documents/Altera/rnafusion -profile docker,eks,std_run -name dev_rna_exp0016-73d51683 --rundir 's3://natera-labdata-rnd-dev-s3/illumina/20260408_LH00995_0025_A23T55JLT4/' --bclconvert_samplesheet 's3://natera-labdata-rnd-dev-s3/illumina/20260408_LH00995_0025_A23T55JLT4/bclconvert_samplesheet.csv' --flowcell_id 20260408_LH00995_0025_A23T55JLT4 --outdir 's3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/dev_rna_exp0016/A23T55JLT4/nfcore_rnafusion__73d51683--20260607-225254'

References

Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. doi: 10.1038/nbt.3820
Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. doi: 10.1038/s41587-020-0439-x
Grüning, B., Dale, R., Sjödin, A., Chapman, B. A., Rowe, J., Tomkins-Tinch, C. H., Valieris, R., Köster, J., & Bioconda Team. (2018). Bioconda: sustainable and comprehensive software distribution for the life sciences. Nature Methods, 15(7), 475–476. doi: 10.1038/s41592-018-0046-7
da Veiga Leprevost, F., Grüning, B. A., Alves Aflitos, S., Röst, H. L., Uszkoreit, J., Barsnes, H., Vaudel, M., Moreno, P., Gatto, L., Weber, J., Bai, M., Jimenez, R. C., Sachsenberg, T., Pfeuffer, J., Vera Alvarez, R., Griss, J., Nesvizhskii, A. I., & Perez-Riverol, Y. (2017). BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics (Oxford, England), 33(16), 2580–2582. doi: 10.1093/bioinformatics/btx192

Notes:

If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
The command above does not include parameters contained in any configs or profiles that may have been used. Ensure the config file is also uploaded with your publication!
You should also cite all software used within this run. Check the "Software Versions" of this report to get version information.

nf-core/rnafusion Workflow Summary

- this information is collected when the pipeline is started.

Core Nextflow options

runName: dev_rna_exp0016-73d51683
containerEngine: docker
launchDir: /code
workDir: /natera-rnd-pltf-dev-nextflow-scratch-01/work
projectDir: /code
userName: nextflow
profile: docker,eks,std_run
configFiles: N/A

Skip steps

skip_picard_qc: true

Input/output options

outdir: s3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/dev_rna_exp0016/A23T55JLT4/nfcore_rnafusion__73d51683--20260607-225254
rundir: s3://natera-labdata-rnd-dev-s3/illumina/20260408_LH00995_0025_A23T55JLT4/
flowcell_id: 20260408_LH00995_0025_A23T55JLT4
strand: reverse
bclconvert_samplesheet: s3://natera-labdata-rnd-dev-s3/illumina/20260408_LH00995_0025_A23T55JLT4/bclconvert_samplesheet.csv
infer_sample_type_map: true
sample_type_map_positive_substring: HD789
genomes_base: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38
read_length: 150
arriba: true
arriba_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/
arriba_ref_blacklist: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz
arriba_ref_cytobands: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv
arriba_ref_known_fusions: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz
arriba_ref_protein_domains: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3
arriba_gtf: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir//ref_annot.gtf
ensembl_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/
fusioncatcher_limitSjdbInsertNsj: 2000000
fusioncatcher_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/fusioncatcher/human_v102/
fusioninspector_limitSjdbInsertNsj: 1000000
fusionreport_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/fusion_report_db/
hgnc_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/hgnc/hgnc_complete_set.txt
hgnc_date: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/hgnc/HGNC-DB-timestamp.txt
starfusion: true
starfusion_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/
starindex_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/star/
starindex_ensembl_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/star/
tools_cutoff: 1

Read trimming options

fastp_trim: true
fastp_reads_to_process: 300000000
adapter_fasta: []

Alignment compression options

cram: starfusion,arriba

Reference genome options

genome: GRCh38
fasta: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.all.fa
fai: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.all.fa.fai
gtf: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.gtf
chrgtf: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.chr.gtf
transcript: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.cdna.all.fa.gz
refflat: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.chr.gtf.refflat
rrna_intervals: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.interval_list

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nf-core/rnafusion Methods Description

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References

Notes:

nf-core/rnafusion Workflow Summary

v1.21

Highlight Samples

Rename Samples

Show / Hide Samples

Mark Duplicates

Sequence Counts

Sequence Quality Histograms

Per Sequence Quality Scores

Per Base Sequence Content

Per Sequence GC Content

Per Base N Content

Sequence Duplication Levels

Overrepresented sequences by sample

Adapter Content

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Sequence Counts

Sequence Quality Histograms

Per Sequence Quality Scores

Per Base Sequence Content

Per Sequence GC Content

Per Base N Content

Sequence Duplication Levels

Overrepresented sequences by sample

Adapter Content

Status Checks