Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d0/4a64e1c37ad9f81009f9c411f8f5b7/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/0d/8632d46b5c73014b3618f0e214184d/1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.SJ.out.tab Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ed/cd1273fd5513b89ea06c357980c98e/1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.Aligned.sortedByCoord.out.bam.bai Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/0d/8632d46b5c73014b3618f0e214184d/1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.Aligned.sortedByCoord.out.bam Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/0d/8632d46b5c73014b3618f0e214184d/1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.Chimeric.out.junction Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d0/4a64e1c37ad9f81009f9c411f8f5b7/.command.run ==> STAGING COMPLETE (7 inputs) 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rate=24585000.00/min [16400000], rate=24600000.00/min [16410000], rate=24615000.00/min [16420000], rate=24630000.00/min [16430000], rate=24645000.00/min [16440000], rate=24660000.00/min [16450000], rate=24675000.00/min [16460000], rate=24690000.00/min [16470000], rate=24705000.00/min [16480000], rate=24720000.00/min [16490000], rate=24735000.00/min [16500000], rate=24750000.00/min [16510000], rate=24765000.00/min [16520000], rate=24780000.00/min [16530000], rate=24795000.00/min [16540000], rate=24810000.00/min [16550000], rate=24219512.20/min [16560000], rate=24234146.34/min [16570000], rate=24248780.49/min [16580000], rate=24263414.63/min [16590000], rate=24278048.78/min [16600000], rate=24292682.93/min [16610000], rate=24307317.07/min [16620000], rate=24321951.22/min [16630000], rate=24336585.37/min [16640000], rate=24351219.51/min [16650000], rate=24365853.66/min 08:49:09 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > 1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.cancer.introns.prelim Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18. -1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.introns: identified 7054 cancer introns 08:49:10 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > 1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.cancer.introns.prelim 08:49:10 : INFO : Running: touch /tmp/nxf.QfX7WXvyPn/1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.chckpts/prelim_introns.ok 08:49:10 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.cancer.introns.prelim --min_total_reads 5 > 1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.cancer.introns 08:49:10 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.cancer.introns.prelim --min_total_reads 5 > 1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.cancer.introns 08:49:10 : INFO : Running: touch /tmp/nxf.QfX7WXvyPn/1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.chckpts/introns_filtered.ok 08:49:10 : INFO : -found 2025 cancer introns 08:49:10 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.introns --cancer_introns 1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed 1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.introns.for_IGV.bed 08:49:10 : INFO : Creating the BED File. 08:49:15 : INFO : Saving Bed File as 1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.introns.for_IGV.bed 08:49:15 : INFO : Execution Time = 0.08 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.introns --cancer_introns 1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed 1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.introns.for_IGV.bed 08:49:15 : INFO : Running: touch /tmp/nxf.QfX7WXvyPn/1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.chckpts/intron_igv_bed.ok 08:49:15 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed 1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.introns.for_IGV.bed --bam 1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.Aligned.sortedByCoord.out.bam --output_prefix 1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1 [W::hts_idx_load3] The index file is older than the data file: 1173_SK8_T1_RNA_SLD_01_A23T55JLT4_1.Aligned.sortedByCoord.out.bam.bai