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rate=16861016.95/min [16590000], rate=16871186.44/min [16600000], rate=16881355.93/min [16610000], rate=16891525.42/min [16620000], rate=16901694.92/min [16630000], rate=16911864.41/min [16640000], rate=16922033.90/min [16650000], rate=16932203.39/min [16660000], rate=16942372.88/min [16670000], rate=16952542.37/min [16680000], rate=16962711.86/min [16690000], rate=16972881.36/min [16700000], rate=16983050.85/min [16710000], rate=16993220.34/min [16720000], rate=17003389.83/min [16730000], rate=17013559.32/min [16740000], rate=17023728.81/min [16750000], rate=17033898.31/min [16760000], rate=17044067.80/min [16770000], rate=17054237.29/min [16780000], rate=16780000.00/min [16790000], rate=16790000.00/min [16800000], rate=16800000.00/min [16810000], rate=16810000.00/min [16820000], rate=16820000.00/min [16830000], rate=16830000.00/min [16840000], rate=16840000.00/min [16850000], rate=16850000.00/min [16860000], rate=16860000.00/min [16870000], rate=16870000.00/min [16880000], rate=16880000.00/min [16890000], rate=16890000.00/min [16900000], rate=16900000.00/min [16910000], rate=16910000.00/min [16920000], rate=16920000.00/min [16930000], rate=16930000.00/min [16940000], rate=16940000.00/min [16950000], rate=16950000.00/min [16960000], rate=16960000.00/min [16970000], rate=16970000.00/min [16980000], rate=16980000.00/min [16990000], rate=16990000.00/min [17000000], rate=17000000.00/min [17010000], rate=17010000.00/min [17020000], rate=17020000.00/min [17030000], rate=17030000.00/min [17040000], rate=17040000.00/min [17050000], rate=17050000.00/min [17060000], rate=17060000.00/min [17070000], rate=17070000.00/min [17080000], rate=17080000.00/min [17090000], rate=16809836.07/min [17100000], rate=16819672.13/min [17110000], rate=16829508.20/min [17120000], rate=16839344.26/min [17130000], rate=16849180.33/min [17140000], rate=16859016.39/min [17150000], rate=16868852.46/min [17160000], rate=16878688.52/min [17170000], rate=16888524.59/min [17180000], rate=16898360.66/min [17190000], rate=16908196.72/min [17200000], rate=16918032.79/min [17210000], rate=16927868.85/min [17220000], rate=16937704.92/min [17230000], rate=16947540.98/min [17240000], rate=16957377.05/min [17250000], rate=16967213.11/min [17260000], rate=16977049.18/min [17270000], rate=16986885.25/min [17280000], rate=16996721.31/min [17290000], rate=17006557.38/min [17300000], rate=17016393.44/min [17310000], rate=17026229.51/min [17320000], rate=17036065.57/min [17330000], rate=17045901.64/min [17340000], rate=17055737.70/min [17350000], rate=17065573.77/min [17360000], rate=17075409.84/min [17370000], rate=17085245.90/min [17380000], rate=17095081.97/min [17390000], rate=16829032.26/min [17400000], rate=16838709.68/min [17410000], rate=16848387.10/min [17420000], rate=16858064.52/min [17430000], rate=16867741.94/min [17440000], rate=16877419.35/min [17450000], rate=16887096.77/min [17460000], rate=16896774.19/min [17470000], rate=16906451.61/min [17480000], rate=16916129.03/min [17490000], rate=16925806.45/min [17500000], rate=16935483.87/min [17510000], rate=16945161.29/min [17520000], rate=16954838.71/min [17530000], rate=16964516.13/min [17540000], rate=16974193.55/min [17550000], rate=16983870.97/min [17560000], rate=16993548.39/min [17570000], rate=17003225.81/min [17580000], rate=17012903.23/min [17590000], rate=17022580.65/min [17600000], rate=17032258.06/min [17610000], rate=17041935.48/min [17620000], rate=17051612.90/min [17630000], rate=17061290.32/min [17640000], rate=17070967.74/min [17650000], rate=16809523.81/min [17660000], rate=16819047.62/min [17670000], rate=16828571.43/min [17680000], rate=16838095.24/min [17690000], rate=16847619.05/min [17700000], rate=16857142.86/min [17710000], rate=16866666.67/min [17720000], rate=16876190.48/min [17730000], rate=16885714.29/min [17740000], rate=16895238.10/min [17750000], rate=16904761.90/min [17760000], rate=16914285.71/min [17770000], rate=16923809.52/min [17780000], rate=16933333.33/min [17790000], rate=16942857.14/min [17800000], rate=16952380.95/min [17810000], rate=16961904.76/min [17820000], rate=16971428.57/min [17830000], rate=16980952.38/min 07:47:09 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18. -1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.introns: identified 1830 cancer introns 07:47:09 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim 07:47:09 : INFO : Running: touch /tmp/nxf.jQFf5pMSUe/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/prelim_introns.ok 07:47:09 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim --min_total_reads 5 > 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns 07:47:09 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns.prelim --min_total_reads 5 > 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns 07:47:09 : INFO : Running: touch /tmp/nxf.jQFf5pMSUe/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/introns_filtered.ok 07:47:10 : INFO : -found 485 cancer introns 07:47:10 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.introns --cancer_introns 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed 07:47:10 : INFO : Creating the BED File. 07:47:12 : INFO : Saving Bed File as 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed 07:47:12 : INFO : Execution Time = 0.04 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.introns --cancer_introns 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed 07:47:12 : INFO : Running: touch /tmp/nxf.jQFf5pMSUe/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/intron_igv_bed.ok 07:47:12 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed --bam 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam --output_prefix 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2 [W::hts_idx_load3] The index file is older than the data file: 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam.bai 07:48:32 : INFO : Execution Time = 1.34 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed --bam 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.Aligned.sortedByCoord.out.bam --output_prefix 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2 07:48:32 : INFO : Running: touch /tmp/nxf.jQFf5pMSUe/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/reads_alignments_extracted.ok 07:48:32 : INFO : Running: samtools sort -o 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.sorted.bam 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.bam 07:48:32 : INFO : Execution Time = 0.00 minutes. CMD: samtools sort -o 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.sorted.bam 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.bam 07:48:32 : INFO : Running: touch /tmp/nxf.jQFf5pMSUe/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/sort_cancer_intron_reads.ok 07:48:32 : INFO : Running: samtools sort -o 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.bam 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.bam [bam_sort_core] merging from 5 files and 1 in-memory blocks... 07:50:12 : INFO : Execution Time = 1.67 minutes. CMD: samtools sort -o 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.bam 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.bam 07:50:12 : INFO : Running: touch /tmp/nxf.jQFf5pMSUe/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/sort_gene_reads.ok 07:50:12 : INFO : Running: /usr/local/src/CTAT-SPLICING/bamsifter/bamsifter -c 50 -i 50 -o 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam.tmp --keep_secondary 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.bam 07:50:36 : INFO : Execution Time = 0.40 minutes. CMD: /usr/local/src/CTAT-SPLICING/bamsifter/bamsifter -c 50 -i 50 -o 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam.tmp --keep_secondary 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.bam 07:50:36 : INFO : Running: touch /tmp/nxf.jQFf5pMSUe/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam.tmp.ok 07:50:36 : INFO : Running: samtools sort -o 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam.tmp 07:50:42 : INFO : Execution Time = 0.10 minutes. CMD: samtools sort -o 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam.tmp 07:50:42 : INFO : Running: touch /tmp/nxf.jQFf5pMSUe/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam.tmpsorted.ok 07:50:42 : INFO : Running: samtools index 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam 07:50:43 : INFO : Execution Time = 0.01 minutes. CMD: samtools index 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam 07:50:43 : INFO : Running: touch /tmp/nxf.jQFf5pMSUe/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.gene_reads.sorted.sifted.bam.indexed.ok 07:50:43 : INFO : Running: samtools index 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.sorted.bam 07:50:43 : INFO : Execution Time = 0.00 minutes. CMD: samtools index 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.sorted.bam 07:50:43 : INFO : Running: touch /tmp/nxf.jQFf5pMSUe/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.cancer_intron_reads.sorted.bam.indexed.ok 07:50:43 : INFO : Running: create_report 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed ctat_genome_lib_build_dir/ref_genome.fa --type junction --output 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.ctat-splicing.igv.html --track-config 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.igv.tracks --info-columns gene variant_name uniquely_mapped multi_mapped TCGA GTEx --title 'CTAT_Splicing: 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2' 07:51:56 : INFO : Execution Time = 1.21 minutes. CMD: create_report 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.introns.for_IGV.bed ctat_genome_lib_build_dir/ref_genome.fa --type junction --output 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.ctat-splicing.igv.html --track-config 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.igv.tracks --info-columns gene variant_name uniquely_mapped multi_mapped TCGA GTEx --title 'CTAT_Splicing: 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2' 07:51:56 : INFO : Running: touch /tmp/nxf.jQFf5pMSUe/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.chckpts/igv_create_html.ok 07:51:56 : INFO : done.