Done. -parsing GTF file: /tmp/nxf.HqtGFoA9pk/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.HqtGFoA9pk/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.HqtGFoA9pk/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2_1.fastp.fastq.gz /tmp/nxf.HqtGFoA9pk/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.HqtGFoA9pk/fi_workdir/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.HqtGFoA9pk/fi_workdir/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.star.sortedByCoord.out.bam * Running CMD: samtools index 1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.star.sortedByCoord.out.bam 09:00:21 : INFO : Done. -parsing GTF file: /tmp/nxf.HqtGFoA9pk/fi_workdir/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.gtf -parsing /tmp/nxf.HqtGFoA9pk/fi_workdir/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] [90000] [100000] [110000] [120000] [130000] [140000] [150000] [160000] [170000] [180000] -done parsing /tmp/nxf.HqtGFoA9pk/fi_workdir/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { ' ** passed ** ' => 102, 'excessive soft clipping' => 1348, 'num genes matched < 2' => 2, 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 2, 'per_id < 96' => 816, 'low complexity anchor region' => 1 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.HqtGFoA9pk/fi_workdir/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] -fusion SPANNING read extraction for scaff: IGFL1P2--HIF3A [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] -fusion SPANNING read extraction for scaff: KATNAL2--SETBP1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] -fusion SPANNING read extraction for scaff: STRN4--ARHGAP35 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] -fusion SPANNING read extraction for scaff: WWC1--TENM2 -filtered reads reasons: $VAR1 = { 'lacks exon overlap' => 578 }; EM: Starting log likelihood: -40.203786 EM: Round [1] log likelihood: -40.203786 EM: Stopping iterations at round 1 due to insufficient improvement in likelihood. -extracting read coordinates from /tmp/nxf.HqtGFoA9pk/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.consolidated.bam into /tmp/nxf.HqtGFoA9pk/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.consolidated.bam.read_coords CMD: touch /tmp/nxf.HqtGFoA9pk/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.HqtGFoA9pk/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.consolidated.bam.read_coords > /tmp/nxf.HqtGFoA9pk/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.HqtGFoA9pk/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.consolidated.bam.frag_coords > /tmp/nxf.HqtGFoA9pk/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.HqtGFoA9pk/fi_workdir/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.HqtGFoA9pk/fi_workdir/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.HqtGFoA9pk/fi_workdir/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.HqtGFoA9pk/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.post_blast_and_promiscuity_filter -done, see /tmp/nxf.HqtGFoA9pk/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -35.262220 EM: Round [1] log likelihood: -35.262220 EM: Stopping iterations at round 1 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.HqtGFoA9pk/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2_1.fastp.fastq.gz: 79906347 Warning - not locating file: /tmp/nxf.HqtGFoA9pk/1173_LAF_T1_RNA_SLD_01_A23T55JLT4_2.gmap_trinity_GG.fusions.gff3.bed ls: cannot access 'IGV_inputs': No such file or directory