Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f7/68045799b0682c60915ff56e90fc73/.command.sh
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/40/5acc309485ca3aa52aefc42f894e1e/1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.Aligned.sortedByCoord.out.bam
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/40/5acc309485ca3aa52aefc42f894e1e/1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.SJ.out.tab
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/40/5acc309485ca3aa52aefc42f894e1e/1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.Chimeric.out.junction
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f7/68045799b0682c60915ff56e90fc73/.command.run
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/3b/cac3a11d8443394fe75d33063eb49e/1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.Aligned.sortedByCoord.out.bam.bai
==> STAGING COMPLETE (7 inputs)
08:40:01 : INFO : -reading targets list: ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
-mapping reads to genes
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[15150000], rate=20200000.00/min 08:40:56 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.cancer.introns.prelim
Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18.
-1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.introns: identified 4662 cancer introns
08:40:57 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.cancer.introns.prelim
08:40:57 : INFO : Running: touch /tmp/nxf.iDAvbs2aOO/1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.chckpts/prelim_introns.ok
08:40:57 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.cancer.introns.prelim --min_total_reads 5 > 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.cancer.introns
08:40:57 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.cancer.introns.prelim --min_total_reads 5 > 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.cancer.introns
08:40:57 : INFO : Running: touch /tmp/nxf.iDAvbs2aOO/1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.chckpts/introns_filtered.ok
08:40:58 : INFO : -found 1309 cancer introns
08:40:58 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.introns --cancer_introns 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.introns.for_IGV.bed
08:40:58 : INFO : Creating the BED File.
08:41:02 : INFO : Saving Bed File as 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.introns.for_IGV.bed
08:41:02 : INFO : Execution Time = 0.07 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.introns --cancer_introns 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.introns.for_IGV.bed
08:41:02 : INFO : Running: touch /tmp/nxf.iDAvbs2aOO/1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.chckpts/intron_igv_bed.ok
08:41:02 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.introns.for_IGV.bed --bam 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.Aligned.sortedByCoord.out.bam --output_prefix 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1
[W::hts_idx_load3] The index file is older than the data file: 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.Aligned.sortedByCoord.out.bam.bai
09:00:17 : INFO : Execution Time = 19.24 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.introns.for_IGV.bed --bam 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.Aligned.sortedByCoord.out.bam --output_prefix 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1
09:00:17 : INFO : Running: touch /tmp/nxf.iDAvbs2aOO/1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.chckpts/reads_alignments_extracted.ok
09:00:17 : INFO : Running: samtools sort -o 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.cancer_intron_reads.sorted.bam 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.cancer_intron_reads.bam
09:00:17 : INFO : Execution Time = 0.01 minutes. CMD: samtools sort -o 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.cancer_intron_reads.sorted.bam 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.cancer_intron_reads.bam
09:00:17 : INFO : Running: touch /tmp/nxf.iDAvbs2aOO/1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.chckpts/sort_cancer_intron_reads.ok
09:00:17 : INFO : Running: samtools sort -o 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.gene_reads.sorted.bam 1173_LV7_T1_RNA_SLD_01_A23T55JLT4_1.gene_reads.bam