[W::hts_idx_load3] The index file is older than the data file: 1173_9C_T1_RNA_SLD_01_A23T55JLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (12115442) splicing reads. 242336 splicing junctions. 182054 known splicing junctions. 60282 novel splicing junctions.
sampling 10% (24230884) splicing reads. 290901 splicing junctions. 195010 known splicing junctions. 95891 novel splicing junctions.
sampling 15% (36346326) splicing reads. 324710 splicing junctions. 202045 known splicing junctions. 122665 novel splicing junctions.
sampling 20% (48461768) splicing reads. 351366 splicing junctions. 206801 known splicing junctions. 144565 novel splicing junctions.
sampling 25% (60577210) splicing reads. 372901 splicing junctions. 210184 known splicing junctions. 162717 novel splicing junctions.
sampling 30% (72692652) splicing reads. 391161 splicing junctions. 212834 known splicing junctions. 178327 novel splicing junctions.
sampling 35% (84808094) splicing reads. 406603 splicing junctions. 215002 known splicing junctions. 191601 novel splicing junctions.
sampling 40% (96923536) splicing reads. 419957 splicing junctions. 216780 known splicing junctions. 203177 novel splicing junctions.
sampling 45% (109038978) splicing reads. 431449 splicing junctions. 218162 known splicing junctions. 213287 novel splicing junctions.
sampling 50% (121154421) splicing reads. 441392 splicing junctions. 219297 known splicing junctions. 222095 novel splicing junctions.
sampling 55% (133269863) splicing reads. 449972 splicing junctions. 220280 known splicing junctions. 229692 novel splicing junctions.
sampling 60% (145385305) splicing reads. 457463 splicing junctions. 221114 known splicing junctions. 236349 novel splicing junctions.
sampling 65% (157500747) splicing reads. 464027 splicing junctions. 221872 known splicing junctions. 242155 novel splicing junctions.
sampling 70% (169616189) splicing reads. 469756 splicing junctions. 222471 known splicing junctions. 247285 novel splicing junctions.
sampling 75% (181731631) splicing reads. 474497 splicing junctions. 223002 known splicing junctions. 251495 novel splicing junctions.
sampling 80% (193847073) splicing reads. 478404 splicing junctions. 223443 known splicing junctions. 254961 novel splicing junctions.
sampling 85% (205962515) splicing reads. 481628 splicing junctions. 223813 known splicing junctions. 257815 novel splicing junctions.
sampling 90% (218077957) splicing reads. 484199 splicing junctions. 224082 known splicing junctions. 260117 novel splicing junctions.
sampling 95% (230193399) splicing reads. 486025 splicing junctions. 224276 known splicing junctions. 261749 novel splicing junctions.
sampling 100% (242308842) splicing reads. 487231 splicing junctions. 224419 known splicing junctions. 262812 novel splicing junctions.