[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00569_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (16527194) splicing reads. 281193 splicing junctions. 191359 known splicing junctions. 89834 novel splicing junctions.
sampling 10% (33054388) splicing reads. 349133 splicing junctions. 204300 known splicing junctions. 144833 novel splicing junctions.
sampling 15% (49581582) splicing reads. 397759 splicing junctions. 211204 known splicing junctions. 186555 novel splicing junctions.
sampling 20% (66108776) splicing reads. 436013 splicing junctions. 215805 known splicing junctions. 220208 novel splicing junctions.
sampling 25% (82635970) splicing reads. 467456 splicing junctions. 219056 known splicing junctions. 248400 novel splicing junctions.
sampling 30% (99163164) splicing reads. 493805 splicing junctions. 221562 known splicing junctions. 272243 novel splicing junctions.
sampling 35% (115690358) splicing reads. 516441 splicing junctions. 223540 known splicing junctions. 292901 novel splicing junctions.
sampling 40% (132217552) splicing reads. 535932 splicing junctions. 225135 known splicing junctions. 310797 novel splicing junctions.
sampling 45% (148744746) splicing reads. 552772 splicing junctions. 226459 known splicing junctions. 326313 novel splicing junctions.
sampling 50% (165271940) splicing reads. 567376 splicing junctions. 227529 known splicing junctions. 339847 novel splicing junctions.
sampling 55% (181799134) splicing reads. 580150 splicing junctions. 228487 known splicing junctions. 351663 novel splicing junctions.
sampling 60% (198326328) splicing reads. 591338 splicing junctions. 229292 known splicing junctions. 362046 novel splicing junctions.
sampling 65% (214853522) splicing reads. 601200 splicing junctions. 229980 known splicing junctions. 371220 novel splicing junctions.
sampling 70% (231380716) splicing reads. 609735 splicing junctions. 230565 known splicing junctions. 379170 novel splicing junctions.
sampling 75% (247907910) splicing reads. 617104 splicing junctions. 231085 known splicing junctions. 386019 novel splicing junctions.
sampling 80% (264435104) splicing reads. 623566 splicing junctions. 231517 known splicing junctions. 392049 novel splicing junctions.
sampling 85% (280962298) splicing reads. 628927 splicing junctions. 231858 known splicing junctions. 397069 novel splicing junctions.
sampling 90% (297489492) splicing reads. 633457 splicing junctions. 232160 known splicing junctions. 401297 novel splicing junctions.
sampling 95% (314016686) splicing reads. 637125 splicing junctions. 232399 known splicing junctions. 404726 novel splicing junctions.
sampling 100% (330543880) splicing reads. 639907 splicing junctions. 232573 known splicing junctions. 407334 novel splicing junctions.