Done. -parsing GTF file: /tmp/nxf.A1Fz39ipCX/tih_rna_sample_00678_B23MVV7LT4_1.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.A1Fz39ipCX/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.A1Fz39ipCX/tih_rna_sample_00678_B23MVV7LT4_1_1.fastp.fastq.gz /tmp/nxf.A1Fz39ipCX/tih_rna_sample_00678_B23MVV7LT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.A1Fz39ipCX/fi_workdir/tih_rna_sample_00678_B23MVV7LT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.A1Fz39ipCX/fi_workdir/tih_rna_sample_00678_B23MVV7LT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam tih_rna_sample_00678_B23MVV7LT4_1.star.sortedByCoord.out.bam * Running CMD: samtools index tih_rna_sample_00678_B23MVV7LT4_1.star.sortedByCoord.out.bam 09:53:58 : INFO : Done. -parsing GTF file: /tmp/nxf.A1Fz39ipCX/fi_workdir/tih_rna_sample_00678_B23MVV7LT4_1.gtf -parsing /tmp/nxf.A1Fz39ipCX/fi_workdir/tih_rna_sample_00678_B23MVV7LT4_1.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] -done parsing /tmp/nxf.A1Fz39ipCX/fi_workdir/tih_rna_sample_00678_B23MVV7LT4_1.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 4, 'exons hit < 2' => 9, 'per_id < 96' => 153, ' ** passed ** ' => 614, 'excessive soft clipping' => 345 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.A1Fz39ipCX/fi_workdir/tih_rna_sample_00678_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] -fusion SPANNING read extraction for scaff: TLK2--AC240565.1 [1000] [2000] [3000] [4000] [5000] -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 -filtered reads reasons: $VAR1 = { 'lacks exon overlap' => 242 }; EM: Starting log likelihood: -511.318664 EM: Round [1] log likelihood: -511.129597 EM: Round [2] log likelihood: -511.126047 EM: Round [3] log likelihood: -511.125982 EM: Stopping iterations at round 3 due to insufficient improvement in likelihood. [bam_sort_core] merging from 0 files and 8 in-memory blocks... -extracting read coordinates from /tmp/nxf.A1Fz39ipCX/tih_rna_sample_00678_B23MVV7LT4_1.consolidated.bam into /tmp/nxf.A1Fz39ipCX/tih_rna_sample_00678_B23MVV7LT4_1.consolidated.bam.read_coords CMD: touch /tmp/nxf.A1Fz39ipCX/tih_rna_sample_00678_B23MVV7LT4_1.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.A1Fz39ipCX/tih_rna_sample_00678_B23MVV7LT4_1.consolidated.bam.read_coords > /tmp/nxf.A1Fz39ipCX/tih_rna_sample_00678_B23MVV7LT4_1.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.A1Fz39ipCX/tih_rna_sample_00678_B23MVV7LT4_1.consolidated.bam.frag_coords > /tmp/nxf.A1Fz39ipCX/tih_rna_sample_00678_B23MVV7LT4_1.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.A1Fz39ipCX/fi_workdir/tih_rna_sample_00678_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.A1Fz39ipCX/fi_workdir/tih_rna_sample_00678_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.A1Fz39ipCX/fi_workdir/tih_rna_sample_00678_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.A1Fz39ipCX/tih_rna_sample_00678_B23MVV7LT4_1.post_blast_and_promiscuity_filter -done, see /tmp/nxf.A1Fz39ipCX/tih_rna_sample_00678_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -511.318664 EM: Round [1] log likelihood: -511.129597 EM: Round [2] log likelihood: -511.126047 EM: Round [3] log likelihood: -511.125982 EM: Stopping iterations at round 3 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.A1Fz39ipCX/tih_rna_sample_00678_B23MVV7LT4_1_1.fastp.fastq.gz: 154820398 Warning - not locating file: /tmp/nxf.A1Fz39ipCX/tih_rna_sample_00678_B23MVV7LT4_1.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000226049.3] WARNING, no entry stored in dbm for [ENSG00000260404.3] WARNING, no entry stored in dbm for [ENSG00000280136.2] ls: cannot access 'IGV_inputs': No such file or directory