Done. -parsing GTF file: /tmp/nxf.k4x1dSR1c2/tih_rna_sample_00671_B23MVV7LT4_1.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.k4x1dSR1c2/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.k4x1dSR1c2/tih_rna_sample_00671_B23MVV7LT4_1_1.fastp.fastq.gz /tmp/nxf.k4x1dSR1c2/tih_rna_sample_00671_B23MVV7LT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.k4x1dSR1c2/fi_workdir/tih_rna_sample_00671_B23MVV7LT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.k4x1dSR1c2/fi_workdir/tih_rna_sample_00671_B23MVV7LT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam tih_rna_sample_00671_B23MVV7LT4_1.star.sortedByCoord.out.bam * Running CMD: samtools index tih_rna_sample_00671_B23MVV7LT4_1.star.sortedByCoord.out.bam 11:08:43 : INFO : Done. -parsing GTF file: /tmp/nxf.k4x1dSR1c2/fi_workdir/tih_rna_sample_00671_B23MVV7LT4_1.gtf -parsing /tmp/nxf.k4x1dSR1c2/fi_workdir/tih_rna_sample_00671_B23MVV7LT4_1.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] [90000] [100000] [110000] [120000] [130000] [140000] [150000] [160000] [170000] [180000] [190000] [200000] [210000] [220000] [230000] [240000] [250000] [260000] [270000] [280000] [290000] [300000] [310000] [320000] [330000] [340000] [350000] [360000] [370000] [380000] [390000] [400000] [410000] [420000] [430000] [440000] [450000] [460000] [470000] [480000] [490000] -done parsing /tmp/nxf.k4x1dSR1c2/fi_workdir/tih_rna_sample_00671_B23MVV7LT4_1.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { 'exons hit < 2' => 1, 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 151, 'low complexity anchor region' => 2, 'num_hits: 3 != num_counted_on_fusion_contigs 2 ' => 16, ' ** passed ** ' => 1662, 'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 6, 'num genes matched < 2' => 37, 'small anchor length' => 39, 'per_id < 96' => 2545, 'num_hits: 5 != num_counted_on_fusion_contigs 4 ' => 8, 'excessive soft clipping' => 4299, 'num_hits: 5 != num_counted_on_fusion_contigs 3 ' => 3, 'num_hits: 6 != num_counted_on_fusion_contigs 3 ' => 6 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.k4x1dSR1c2/fi_workdir/tih_rna_sample_00671_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] -fusion SPANNING read extraction for scaff: ACSS1--MRPS11P1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] -fusion SPANNING read extraction for scaff: ACSS1--AL133465.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] -fusion SPANNING read extraction for scaff: ACSS1--RN7SL607P [1000] -fusion SPANNING read extraction for scaff: AL669831.4--SEPTIN14 [1000] -fusion SPANNING read extraction for scaff: AP006294.2--ENPP7P3 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] -fusion SPANNING read extraction for scaff: GUSBP13--NAIP [1000] [2000] -fusion SPANNING read extraction for scaff: KLRC4--KLRK1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] -fusion SPANNING read extraction for scaff: PGBD2--ZNF692 [1000] [2000] [3000] [4000] [5000] [6000] -fusion SPANNING read extraction for scaff: PPP2R2B--STK32A [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] -fusion SPANNING read extraction for scaff: PRSS27--TPSB2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] -fusion SPANNING read extraction for scaff: SCRN3--SLC44A5 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] [103000] [104000] [105000] [106000] [107000] [108000] [109000] [110000] [111000] [112000] [113000] [114000] [115000] [116000] -fusion SPANNING read extraction for scaff: STX16--NPEPL1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] -fusion SPANNING read extraction for scaff: TCERG1--PPP2R2B [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] -fusion SPANNING read extraction for scaff: TLK2--FAM157A [1000] [2000] [3000] [4000] [5000] -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] -fusion SPANNING read extraction for scaff: VWA8--LINC01160 -filtered reads reasons: $VAR1 = { 'lacks exon overlap' => 3325 }; EM: Starting log likelihood: -2515.442392 EM: Round [1] log likelihood: -2506.233678 EM: Round [2] log likelihood: -2506.102609 EM: Round [3] log likelihood: -2506.102009 EM: Round [4] log likelihood: -2506.102006 EM: Stopping iterations at round 4 due to insufficient improvement in likelihood. [bam_sort_core] merging from 0 files and 8 in-memory blocks... -extracting read coordinates from /tmp/nxf.k4x1dSR1c2/tih_rna_sample_00671_B23MVV7LT4_1.consolidated.bam into /tmp/nxf.k4x1dSR1c2/tih_rna_sample_00671_B23MVV7LT4_1.consolidated.bam.read_coords CMD: touch /tmp/nxf.k4x1dSR1c2/tih_rna_sample_00671_B23MVV7LT4_1.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.k4x1dSR1c2/tih_rna_sample_00671_B23MVV7LT4_1.consolidated.bam.read_coords > /tmp/nxf.k4x1dSR1c2/tih_rna_sample_00671_B23MVV7LT4_1.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.k4x1dSR1c2/tih_rna_sample_00671_B23MVV7LT4_1.consolidated.bam.frag_coords > /tmp/nxf.k4x1dSR1c2/tih_rna_sample_00671_B23MVV7LT4_1.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.k4x1dSR1c2/fi_workdir/tih_rna_sample_00671_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.k4x1dSR1c2/fi_workdir/tih_rna_sample_00671_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.k4x1dSR1c2/fi_workdir/tih_rna_sample_00671_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.k4x1dSR1c2/tih_rna_sample_00671_B23MVV7LT4_1.post_blast_and_promiscuity_filter -done, see /tmp/nxf.k4x1dSR1c2/tih_rna_sample_00671_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -2321.366747 EM: Round [1] log likelihood: -2320.637859 EM: Round [2] log likelihood: -2320.637563 EM: Round [3] log likelihood: -2320.637562 EM: Stopping iterations at round 3 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.k4x1dSR1c2/tih_rna_sample_00671_B23MVV7LT4_1_1.fastp.fastq.gz: 296369001 Warning - not locating file: /tmp/nxf.k4x1dSR1c2/tih_rna_sample_00671_B23MVV7LT4_1.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000198237.8] WARNING, no entry stored in dbm for [ENSG00000230092.7] WARNING, no entry stored in dbm for [ENSG00000236438.7] WARNING, no entry stored in dbm for [ENSG00000226049.3] WARNING, no entry stored in dbm for [ENSG00000260404.3] WARNING, no entry stored in dbm for [ENSG00000284639.1] WARNING, no entry stored in dbm for [ENSG00000248618.1] WARNING, no entry stored in dbm for [ENSG00000231346.5] ls: cannot access 'IGV_inputs': No such file or directory