Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_genome.fa Downloading: s3://natera-platform-sandbox/pipeline-resources/Homo_sapiens_assembly38.dbsnp138.vcf.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ce/6476ea06ac3b46b7b1550ba5bee51a/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/73/0ad11a5986b7874699d98dd39842f9/tih_rna_sample_00584_B23MVV7LT4_1.md.bai Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/73/0ad11a5986b7874699d98dd39842f9/tih_rna_sample_00584_B23MVV7LT4_1.md.bam Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_genome.dict Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/gatk4/dbsnp_hg38_contam_sites.bed Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ce/6476ea06ac3b46b7b1550ba5bee51a/.command.run Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai Downloading: s3://natera-platform-sandbox/pipeline-resources/beds/twist/Twist_rna_exome_AND_fusion_hg38.bed Downloading: s3://natera-platform-sandbox/pipeline-resources/Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi ==> STAGING COMPLETE (11 inputs) + mkdir tih_rna_sample_00584_B23MVV7LT4_1 + all_qc -bam tih_rna_sample_00584_B23MVV7LT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder tih_rna_sample_00584_B23MVV7LT4_1 -reference ref_genome.fa -region chr20 mkdir -p tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md/ cat Twist_rna_exome_AND_fusion_hg38.bed | cut -f 1-3 | bedtools sort -g ref_genome.fa.fai | bedtools merge > tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md/full_bed.bed seqtool depth -bam tih_rna_sample_00584_B23MVV7LT4_1.md.bam -bed tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md/full_bed.bed -threads 4 -bam_threads 2 | bgzip -c > tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md/tih_rna_sample_00584_B23MVV7LT4_1.md.depth.gz saturation_plot -bams tih_rna_sample_00584_B23MVV7LT4_1.md.bam -region chr20 -output tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md/saturation_plot.png -threads 1 seqtool saturation_metric -at 1000000 -region chr20 -bam_threads 1 tih_rna_sample_00584_B23MVV7LT4_1.md.bam > tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md/saturation_qc.tsv fragment_size -bams tih_rna_sample_00584_B23MVV7LT4_1.md.bam -region chr20 -output_prefix tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md/fragsize -threads 1 samtools mpileup -B --min-MQ 60 --min-BQ 30 -f ref_genome.fa -l dbsnp_hg38_contam_sites.bed tih_rna_sample_00584_B23MVV7LT4_1.md.bam > tih_rna_sample_00584_B23MVV7LT4_1.md.mpileup && contamination -input tih_rna_sample_00584_B23MVV7LT4_1.md.mpileup -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz > tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md/contamination.tsv [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai 0it [00:00, ?it/s] 166027it [00:00, 1660133.05it/s] 346200it [00:00, 1743369.73it/s] 528974it [00:00, 1781853.67it/s][W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai 712860it [00:00, 1804323.36it/s] 894496it [00:00, 1808630.12it/s][W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai 1075360it [00:00, 1808181.17it/s] 1257606it [00:00, 1812704.83it/s][W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai 1441369it [00:00, 1820608.51it/s] 1623431it [00:00, 1819076.72it/s] 1807478it [00:01, 1825650.46it/s] 1900456it [00:01, 1808099.18it/s] [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai tabix -s 1 -b 2 -e 2 tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md/tih_rna_sample_00584_B23MVV7LT4_1.md.depth.gz seqtool depth_hist -nbins 500 tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md/tih_rna_sample_00584_B23MVV7LT4_1.md.depth.gz > tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md/tih_rna_sample_00584_B23MVV7LT4_1.md.depth_hist.tsv cat tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md/tih_rna_sample_00584_B23MVV7LT4_1.md.depth_hist.tsv | plot -x 0 -xlabel Depth -ylabel "Number of Sites" -xlim 0 2000 -title tih_rna_sample_00584_B23MVV7LT4_1.md -output tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md/tih_rna_sample_00584_B23MVV7LT4_1.md.depth.png [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi seqtool read_qc -bed Twist_rna_exome_AND_fusion_hg38.bed -n_threads 1 -bam_threads 2 tih_rna_sample_00584_B23MVV7LT4_1.md.bam > tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md/read_qc.Twist_rna_exome_AND_fusion_hg38.bed.tsv seqtool reads_per_target -bed Twist_rna_exome_AND_fusion_hg38.bed -threads 1 -bam_threads 2 tih_rna_sample_00584_B23MVV7LT4_1.md.bam > tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md/reads_per_target.Twist_rna_exome_AND_fusion_hg38.bed.tsv seqtool depth_stats -bed Twist_rna_exome_AND_fusion_hg38.bed tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md/tih_rna_sample_00584_B23MVV7LT4_1.md.depth.gz> tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md/depth_stats.Twist_rna_exome_AND_fusion_hg38.bed.tsv seqtool gc_bias -reference ref_genome.fa -bed Twist_rna_exome_AND_fusion_hg38.bed -bin_size 0.05 -threads 1 -bam_threads 2 tih_rna_sample_00584_B23MVV7LT4_1.md.bam > tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md/gc_bias_qc.Twist_rna_exome_AND_fusion_hg38.bed.tsv [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.md.bai cat tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md/gc_bias_qc.Twist_rna_exome_AND_fusion_hg38.bed.tsv | plot -x 0 -header infer -ylim 0 2 -title "tih_rna_sample_00584_B23MVV7LT4_1.md" -suptitle "Twist_rna_exome_AND_fusion_hg38.bed" -output "tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md/gc_bias.Twist_rna_exome_AND_fusion_hg38.bed.png" -ylabel "Fold enrichment / depletion" -xlabel "GC Quantile" echo `date` > tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md/qc_complete.txt + merge_qc -folders tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1.md -output tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1_full_basic_stats.tsv + head -n1 tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed tih_rna_sample_00584_B23MVV7LT4_1/tih_rna_sample_00584_B23MVV7LT4_1_full_basic_stats.tsv