File Info

Filename
fi_workdir/Log.out
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/54/71b706cb2048deb8c3cbaade321fe9/fi_workdir/Log.out
Size
21.0 KB
Attempt
STAR version=2.7.8a
STAR compilation time,server,dir=Sat Feb 20 13:34:37 EST 2021 vega:/home/dobin/data/STAR/STARcode/STAR.master/source
##### Command Line:
/usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.6DJG8gVLqU/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.6DJG8gVLqU/tih_rna_sample_00660_B23MVV7LT4_1_1.fastp.fastq.gz /tmp/nxf.6DJG8gVLqU/tih_rna_sample_00660_B23MVV7LT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.6DJG8gVLqU/fi_workdir/tih_rna_sample_00660_B23MVV7LT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.6DJG8gVLqU/fi_workdir/tih_rna_sample_00660_B23MVV7LT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand "gunzip -c"
##### Initial USER parameters from Command Line:
###### All USER parameters from Command Line:
runThreadN                    8     ~RE-DEFINED
genomeDir                     /tmp/nxf.6DJG8gVLqU/ctat_genome_lib_build_dir/ref_genome.fa.star.idx     ~RE-DEFINED
outSAMtype                    BAM   SortedByCoordinate        ~RE-DEFINED
twopassMode                   None     ~RE-DEFINED
alignSJDBoverhangMin          10     ~RE-DEFINED
genomeSuffixLengthMax         10000     ~RE-DEFINED
limitBAMsortRAM               47271261705     ~RE-DEFINED
alignInsertionFlush           Right     ~RE-DEFINED
alignMatesGapMax              100000     ~RE-DEFINED
alignIntronMax                100000     ~RE-DEFINED
outSAMattributes              All        ~RE-DEFINED
readFilesIn                   /tmp/nxf.6DJG8gVLqU/tih_rna_sample_00660_B23MVV7LT4_1_1.fastp.fastq.gz   /tmp/nxf.6DJG8gVLqU/tih_rna_sample_00660_B23MVV7LT4_1_2.fastp.fastq.gz        ~RE-DEFINED
genomeFastaFiles              /tmp/nxf.6DJG8gVLqU/fi_workdir/tih_rna_sample_00660_B23MVV7LT4_1.fa        ~RE-DEFINED
outSAMfilter                  KeepAllAddedReferences        ~RE-DEFINED
sjdbGTFfile                   /tmp/nxf.6DJG8gVLqU/fi_workdir/tih_rna_sample_00660_B23MVV7LT4_1.gtf     ~RE-DEFINED
alignSJstitchMismatchNmax     5   -1   5   5        ~RE-DEFINED
scoreGapNoncan                -6     ~RE-DEFINED
readFilesCommand              "gunzip -c"        ~RE-DEFINED
##### Finished reading parameters from all sources

##### Final user re-defined parameters-----------------:
runThreadN                        8
genomeDir                         /tmp/nxf.6DJG8gVLqU/ctat_genome_lib_build_dir/ref_genome.fa.star.idx
genomeFastaFiles                  /tmp/nxf.6DJG8gVLqU/fi_workdir/tih_rna_sample_00660_B23MVV7LT4_1.fa   
genomeSuffixLengthMax             10000
readFilesIn                       /tmp/nxf.6DJG8gVLqU/tih_rna_sample_00660_B23MVV7LT4_1_1.fastp.fastq.gz   /tmp/nxf.6DJG8gVLqU/tih_rna_sample_00660_B23MVV7LT4_1_2.fastp.fastq.gz   
readFilesCommand                  "gunzip -c"   
limitBAMsortRAM                   47271261705
outSAMtype                        BAM   SortedByCoordinate   
outSAMattributes                  All   
outSAMfilter                      KeepAllAddedReferences   
scoreGapNoncan                    -6
alignIntronMax                    100000
alignMatesGapMax                  100000
alignSJDBoverhangMin              10
alignSJstitchMismatchNmax         5   -1   5   5   
alignInsertionFlush               Right
sjdbGTFfile                       /tmp/nxf.6DJG8gVLqU/fi_workdir/tih_rna_sample_00660_B23MVV7LT4_1.gtf
twopassMode                       None

-------------------------------
##### Final effective command line:
/usr/local/bin/STAR   --runThreadN 8   --genomeDir /tmp/nxf.6DJG8gVLqU/ctat_genome_lib_build_dir/ref_genome.fa.star.idx   --genomeFastaFiles /tmp/nxf.6DJG8gVLqU/fi_workdir/tih_rna_sample_00660_B23MVV7LT4_1.fa      --genomeSuffixLengthMax 10000   --readFilesIn /tmp/nxf.6DJG8gVLqU/tih_rna_sample_00660_B23MVV7LT4_1_1.fastp.fastq.gz   /tmp/nxf.6DJG8gVLqU/tih_rna_sample_00660_B23MVV7LT4_1_2.fastp.fastq.gz      --readFilesCommand "gunzip -c"      --limitBAMsortRAM 47271261705   --outSAMtype BAM   SortedByCoordinate      --outSAMattributes All      --outSAMfilter KeepAllAddedReferences      --scoreGapNoncan -6   --alignIntronMax 100000   --alignMatesGapMax 100000   --alignSJDBoverhangMin 10   --alignSJstitchMismatchNmax 5   -1   5   5      --alignInsertionFlush Right   --sjdbGTFfile /tmp/nxf.6DJG8gVLqU/fi_workdir/tih_rna_sample_00660_B23MVV7LT4_1.gtf   --twopassMode None
----------------------------------------

Number of fastq files for each mate = 1

   Input read files for mate 1 :
-rw-r--r--. 1 1000 root 13085992641 Jun  8 10:20 /tmp/nxf.6DJG8gVLqU/tih_rna_sample_00660_B23MVV7LT4_1_1.fastp.fastq.gz

   readsCommandsFile:
exec > "./_STARtmp/tmp.fifo.read1"
echo FILE 0
gunzip -c      "/tmp/nxf.6DJG8gVLqU/tih_rna_sample_00660_B23MVV7LT4_1_1.fastp.fastq.gz"


   Input read files for mate 2 :
-rw-r--r--. 1 1000 root 13143546011 Jun  8 10:20 /tmp/nxf.6DJG8gVLqU/tih_rna_sample_00660_B23MVV7LT4_1_2.fastp.fastq.gz

   readsCommandsFile:
exec > "./_STARtmp/tmp.fifo.read2"
echo FILE 0
gunzip -c      "/tmp/nxf.6DJG8gVLqU/tih_rna_sample_00660_B23MVV7LT4_1_2.fastp.fastq.gz"

ParametersSolo: --soloCellFilterType CellRanger2.2 filtering parameters:  3000 0.99 10
Finished loading and checking parameters
Reading genome generation parameters:
### STAR   --runMode genomeGenerate      --runThreadN 4   --genomeDir /seq/RNASEQ/__ctat_genome_lib_building/Mar2021/GRCh38_gencode_v37_CTAT_lib_Mar012021/ctat_genome_lib_build_dir/ref_genome.fa.star.idx   --genomeFastaFiles /seq/RNASEQ/__ctat_genome_lib_building/Mar2021/GRCh38_gencode_v37_CTAT_lib_Mar012021/GRCh38.primary_assembly.genome.fa.pseudo_masked.fa      --genomeChrBinNbits 16   --limitGenomeGenerateRAM 40419136213   --sjdbGTFfile /seq/RNASEQ/__ctat_genome_lib_building/Mar2021/GRCh38_gencode_v37_CTAT_lib_Mar012021/gencode.v37.annotation.gtf.revised.custom.gtf   --sjdbOverhang 150
### GstrandBit=32
versionGenome                 2.7.4a     ~RE-DEFINED
genomeType                    Full     ~RE-DEFINED
genomeFastaFiles              /seq/RNASEQ/__ctat_genome_lib_building/Mar2021/GRCh38_gencode_v37_CTAT_lib_Mar012021/GRCh38.primary_assembly.genome.fa.pseudo_masked.fa        ~RE-DEFINED
genomeSAindexNbases           14     ~RE-DEFINED
genomeChrBinNbits             16     ~RE-DEFINED
genomeSAsparseD               1     ~RE-DEFINED
genomeTransformType           None     ~RE-DEFINED
genomeTransformVCF            -     ~RE-DEFINED
sjdbOverhang                  150     ~RE-DEFINED
sjdbFileChrStartEnd           -        ~RE-DEFINED
sjdbGTFfile                   /seq/RNASEQ/__ctat_genome_lib_building/Mar2021/GRCh38_gencode_v37_CTAT_lib_Mar012021/gencode.v37.annotation.gtf.revised.custom.gtf     ~RE-DEFINED
sjdbGTFchrPrefix              -     ~RE-DEFINED
sjdbGTFfeatureExon            exon     ~RE-DEFINED
sjdbGTFtagExonParentTranscripttranscript_id     ~RE-DEFINED
sjdbGTFtagExonParentGene      gene_id     ~RE-DEFINED
sjdbInsertSave                Basic     ~RE-DEFINED
genomeFileSizes               3225043118   25262353185        ~RE-DEFINED
Genome version is compatible with current STAR
Number of real (reference) chromosomes= 194
1	chr1	248956422	0
2	chr2	242193529	248971264
3	chr3	198295559	491192320
4	chr4	190214555	689504256
5	chr5	181538259	879755264
6	chr6	170805979	1061355520
7	chr7	159345973	1232207872
8	chr8	145138636	1391591424
9	chr9	138394717	1536753664
10	chr10	133797422	1675165696
11	chr11	135086622	1808990208
12	chr12	133275309	1944125440
13	chr13	114364328	2077425664
14	chr14	107043718	2191851520
15	chr15	101991189	2298937344
16	chr16	90338345	2400976896
17	chr17	83257441	2491351040
18	chr18	80373285	2574647296
19	chr19	58617616	2655059968
20	chr20	64444167	2713714688
21	chr21	46709983	2778202112
22	chr22	50818468	2824929280
23	chrX	156040895	2875785216
24	chrY	57227415	3031826432
25	chrM	16569	3089104896
26	GL000008.2	209709	3089170432
27	GL000009.2	201709	3089432576
28	GL000194.1	191469	3089694720
29	GL000195.1	182896	3089891328
30	GL000205.2	185591	3090087936
31	GL000208.1	92689	3090284544
32	GL000213.1	164239	3090415616
33	GL000214.1	137718	3090612224
34	GL000216.2	176608	3090808832
35	GL000218.1	161147	3091005440
36	GL000219.1	179198	3091202048
37	GL000220.1	161802	3091398656
38	GL000221.1	155397	3091595264
39	GL000224.1	179693	3091791872
40	GL000225.1	211173	3091988480
41	GL000226.1	15008	3092250624
42	KI270302.1	2274	3092316160
43	KI270303.1	1942	3092381696
44	KI270304.1	2165	3092447232
45	KI270305.1	1472	3092512768
46	KI270310.1	1201	3092578304
47	KI270311.1	12399	3092643840
48	KI270312.1	998	3092709376
49	KI270315.1	2276	3092774912
50	KI270316.1	1444	3092840448
51	KI270317.1	37690	3092905984
52	KI270320.1	4416	3092971520
53	KI270322.1	21476	3093037056
54	KI270329.1	1040	3093102592
55	KI270330.1	1652	3093168128
56	KI270333.1	2699	3093233664
57	KI270334.1	1368	3093299200
58	KI270335.1	1048	3093364736
59	KI270336.1	1026	3093430272
60	KI270337.1	1121	3093495808
61	KI270338.1	1428	3093561344
62	KI270340.1	1428	3093626880
63	KI270362.1	3530	3093692416
64	KI270363.1	1803	3093757952
65	KI270364.1	2855	3093823488
66	KI270366.1	8320	3093889024
67	KI270371.1	2805	3093954560
68	KI270372.1	1650	3094020096
69	KI270373.1	1451	3094085632
70	KI270374.1	2656	3094151168
71	KI270375.1	2378	3094216704
72	KI270376.1	1136	3094282240
73	KI270378.1	1048	3094347776
74	KI270379.1	1045	3094413312
75	KI270381.1	1930	3094478848
76	KI270382.1	4215	3094544384
77	KI270383.1	1750	3094609920
78	KI270384.1	1658	3094675456
79	KI270385.1	990	3094740992
80	KI270386.1	1788	3094806528
81	KI270387.1	1537	3094872064
82	KI270388.1	1216	3094937600
83	KI270389.1	1298	3095003136
84	KI270390.1	2387	3095068672
85	KI270391.1	1484	3095134208
86	KI270392.1	971	3095199744
87	KI270393.1	1308	3095265280
88	KI270394.1	970	3095330816
89	KI270395.1	1143	3095396352
90	KI270396.1	1880	3095461888
91	KI270411.1	2646	3095527424
92	KI270412.1	1179	3095592960
93	KI270414.1	2489	3095658496
94	KI270417.1	2043	3095724032
95	KI270418.1	2145	3095789568
96	KI270419.1	1029	3095855104
97	KI270420.1	2321	3095920640
98	KI270422.1	1445	3095986176
99	KI270423.1	981	3096051712
100	KI270424.1	2140	3096117248
101	KI270425.1	1884	3096182784
102	KI270429.1	1361	3096248320
103	KI270435.1	92983	3096313856
104	KI270438.1	112505	3096444928
105	KI270442.1	392061	3096576000
106	KI270448.1	7992	3096969216
107	KI270465.1	1774	3097034752
108	KI270466.1	1233	3097100288
109	KI270467.1	3920	3097165824
110	KI270468.1	4055	3097231360
111	KI270507.1	5353	3097296896
112	KI270508.1	1951	3097362432
113	KI270509.1	2318	3097427968
114	KI270510.1	2415	3097493504
115	KI270511.1	8127	3097559040
116	KI270512.1	22689	3097624576
117	KI270515.1	6361	3097690112
118	KI270516.1	1300	3097755648
119	KI270517.1	3253	3097821184
120	KI270518.1	2186	3097886720
121	KI270519.1	138126	3097952256
122	KI270521.1	7642	3098148864
123	KI270522.1	5674	3098214400
124	KI270528.1	2983	3098279936
125	KI270529.1	1899	3098345472
126	KI270530.1	2168	3098411008
127	KI270538.1	91309	3098476544
128	KI270539.1	993	3098607616
129	KI270544.1	1202	3098673152
130	KI270548.1	1599	3098738688
131	KI270579.1	31033	3098804224
132	KI270580.1	1553	3098869760
133	KI270581.1	7046	3098935296
134	KI270582.1	6504	3099000832
135	KI270583.1	1400	3099066368
136	KI270584.1	4513	3099131904
137	KI270587.1	2969	3099197440
138	KI270588.1	6158	3099262976
139	KI270589.1	44474	3099328512
140	KI270590.1	4685	3099394048
141	KI270591.1	5796	3099459584
142	KI270593.1	3041	3099525120
143	KI270706.1	175055	3099590656
144	KI270707.1	32032	3099787264
145	KI270708.1	127682	3099852800
146	KI270709.1	66860	3099983872
147	KI270710.1	40176	3100114944
148	KI270711.1	42210	3100180480
149	KI270712.1	176043	3100246016
150	KI270713.1	40745	3100442624
151	KI270714.1	41717	3100508160
152	KI270715.1	161471	3100573696
153	KI270716.1	153799	3100770304
154	KI270717.1	40062	3100966912
155	KI270718.1	38054	3101032448
156	KI270719.1	176845	3101097984
157	KI270720.1	39050	3101294592
158	KI270721.1	100316	3101360128
159	KI270722.1	194050	3101491200
160	KI270723.1	38115	3101687808
161	KI270724.1	39555	3101753344
162	KI270725.1	172810	3101818880
163	KI270726.1	43739	3102015488
164	KI270727.1	448248	3102081024
165	KI270728.1	1872759	3102539776
166	KI270729.1	280839	3104440320
167	KI270730.1	112551	3104768000
168	KI270731.1	150754	3104899072
169	KI270732.1	41543	3105095680
170	KI270733.1	179772	3105161216
171	KI270734.1	165050	3105357824
172	KI270735.1	42811	3105554432
173	KI270736.1	181920	3105619968
174	KI270737.1	103838	3105816576
175	KI270738.1	99375	3105947648
176	KI270739.1	73985	3106078720
177	KI270740.1	37240	3106209792
178	KI270741.1	157432	3106275328
179	KI270742.1	186739	3106471936
180	KI270743.1	210658	3106668544
181	KI270744.1	168472	3106930688
182	KI270745.1	41891	3107127296
183	KI270746.1	66486	3107192832
184	KI270747.1	198735	3107323904
185	KI270748.1	93321	3107586048
186	KI270749.1	158759	3107717120
187	KI270750.1	148850	3107913728
188	KI270751.1	150742	3108110336
189	KI270752.1	27745	3108306944
190	KI270753.1	62944	3108372480
191	KI270754.1	40191	3108438016
192	KI270755.1	36723	3108503552
193	KI270756.1	79590	3108569088
194	KI270757.1	71251	3108700160
--sjdbOverhang = 150 taken from the generated genome
Started loading the genome: Mon Jun  8 10:21:04 2026

Genome: size given as a parameter = 3225043118
SA: size given as a parameter = 25262353185
SAindex: size given as a parameter = 1
Read from SAindex: pGe.gSAindexNbases=14  nSAi=357913940
nGenome=3225043118;  nSAbyte=25262353185
GstrandBit=32   SA number of indices=6124206832
/tmp/nxf.6DJG8gVLqU/fi_workdir/tih_rna_sample_00660_B23MVV7LT4_1.fa : chr # 194  "MAPKAPK5--ACAD10" chrStart: 3108831232
/tmp/nxf.6DJG8gVLqU/fi_workdir/tih_rna_sample_00660_B23MVV7LT4_1.fa : chr # 195  "PCDHA3--PCDHAC2" chrStart: 3108896768
/tmp/nxf.6DJG8gVLqU/fi_workdir/tih_rna_sample_00660_B23MVV7LT4_1.fa : chr # 196  "PCDHA3--PCDHAC1" chrStart: 3108962304
/tmp/nxf.6DJG8gVLqU/fi_workdir/tih_rna_sample_00660_B23MVV7LT4_1.fa : chr # 197  "PCDHA3--PCDHA13" chrStart: 3109027840
/tmp/nxf.6DJG8gVLqU/fi_workdir/tih_rna_sample_00660_B23MVV7LT4_1.fa : chr # 198  "STX16--NPEPL1" chrStart: 3109093376
/tmp/nxf.6DJG8gVLqU/fi_workdir/tih_rna_sample_00660_B23MVV7LT4_1.fa : chr # 199  "TLK2--AC240565.1" chrStart: 3109158912
/tmp/nxf.6DJG8gVLqU/fi_workdir/tih_rna_sample_00660_B23MVV7LT4_1.fa : chr # 200  "TLK2P1--AC110079.1" chrStart: 3109224448
/tmp/nxf.6DJG8gVLqU/fi_workdir/tih_rna_sample_00660_B23MVV7LT4_1.fa : chr # 201  "TVP23C--CDRT4" chrStart: 3109289984
Chromosome sequence lengths: 
chr1	248956422
chr2	242193529
chr3	198295559
chr4	190214555
chr5	181538259
chr6	170805979
chr7	159345973
chr8	145138636
chr9	138394717
chr10	133797422
chr11	135086622
chr12	133275309
chr13	114364328
chr14	107043718
chr15	101991189
chr16	90338345
chr17	83257441
chr18	80373285
chr19	58617616
chr20	64444167
chr21	46709983
chr22	50818468
chrX	156040895
chrY	57227415
chrM	16569
GL000008.2	209709
GL000009.2	201709
GL000194.1	191469
GL000195.1	182896
GL000205.2	185591
GL000208.1	92689
GL000213.1	164239
GL000214.1	137718
GL000216.2	176608
GL000218.1	161147
GL000219.1	179198
GL000220.1	161802
GL000221.1	155397
GL000224.1	179693
GL000225.1	211173
GL000226.1	15008
KI270302.1	2274
KI270303.1	1942
KI270304.1	2165
KI270305.1	1472
KI270310.1	1201
KI270311.1	12399
KI270312.1	998
KI270315.1	2276
KI270316.1	1444
KI270317.1	37690
KI270320.1	4416
KI270322.1	21476
KI270329.1	1040
KI270330.1	1652
KI270333.1	2699
KI270334.1	1368
KI270335.1	1048
KI270336.1	1026
KI270337.1	1121
KI270338.1	1428
KI270340.1	1428
KI270362.1	3530
KI270363.1	1803
KI270364.1	2855
KI270366.1	8320
KI270371.1	2805
KI270372.1	1650
KI270373.1	1451
KI270374.1	2656
KI270375.1	2378
KI270376.1	1136
KI270378.1	1048
KI270379.1	1045
KI270381.1	1930
KI270382.1	4215
KI270383.1	1750
KI270384.1	1658
KI270385.1	990
KI270386.1	1788
KI270387.1	1537
KI270388.1	1216
KI270389.1	1298
KI270390.1	2387
KI270391.1	1484
KI270392.1	971
KI270393.1	1308
KI270394.1	970
KI270395.1	1143
KI270396.1	1880
KI270411.1	2646
KI270412.1	1179
KI270414.1	2489
KI270417.1	2043
KI270418.1	2145
KI270419.1	1029
KI270420.1	2321
KI270422.1	1445
KI270423.1	981
KI270424.1	2140
KI270425.1	1884
KI270429.1	1361
KI270435.1	92983
KI270438.1	112505
KI270442.1	392061
KI270448.1	7992
KI270465.1	1774
KI270466.1	1233
KI270467.1	3920
KI270468.1	4055
KI270507.1	5353
KI270508.1	1951
KI270509.1	2318
KI270510.1	2415
KI270511.1	8127
KI270512.1	22689
KI270515.1	6361
KI270516.1	1300
KI270517.1	3253
KI270518.1	2186
KI270519.1	138126
KI270521.1	7642
KI270522.1	5674
KI270528.1	2983
KI270529.1	1899
KI270530.1	2168
KI270538.1	91309
KI270539.1	993
KI270544.1	1202
KI270548.1	1599
KI270579.1	31033
KI270580.1	1553
KI270581.1	7046
KI270582.1	6504
KI270583.1	1400
KI270584.1	4513
KI270587.1	2969
KI270588.1	6158
KI270589.1	44474
KI270590.1	4685
KI270591.1	5796
KI270593.1	3041
KI270706.1	175055
KI270707.1	32032
KI270708.1	127682
KI270709.1	66860
KI270710.1	40176
KI270711.1	42210
KI270712.1	176043
KI270713.1	40745
KI270714.1	41717
KI270715.1	161471
KI270716.1	153799
KI270717.1	40062
KI270718.1	38054
KI270719.1	176845
KI270720.1	39050
KI270721.1	100316
KI270722.1	194050
KI270723.1	38115
KI270724.1	39555
KI270725.1	172810
KI270726.1	43739
KI270727.1	448248
KI270728.1	1872759
KI270729.1	280839
KI270730.1	112551
KI270731.1	150754
KI270732.1	41543
KI270733.1	179772
KI270734.1	165050
KI270735.1	42811
KI270736.1	181920
KI270737.1	103838
KI270738.1	99375
KI270739.1	73985
KI270740.1	37240
KI270741.1	157432
KI270742.1	186739
KI270743.1	210658
KI270744.1	168472
KI270745.1	41891
KI270746.1	66486
KI270747.1	198735
KI270748.1	93321
KI270749.1	158759
KI270750.1	148850
KI270751.1	150742
KI270752.1	27745
KI270753.1	62944
KI270754.1	40191
KI270755.1	36723
KI270756.1	79590
KI270757.1	71251
MAPKAPK5--ACAD10	59156
PCDHA3--PCDHAC2	20336
PCDHA3--PCDHAC1	19610
PCDHA3--PCDHA13	18035
STX16--NPEPL1	33741
TLK2--AC240565.1	49301
TLK2P1--AC110079.1	20707
TVP23C--CDRT4	26887
Shared memory is not used for genomes. Allocated a private copy of the genome.
Genome file size: 3225043118 bytes; state: good=1 eof=0 fail=0 bad=0
Loading Genome ... done! state: good=1 eof=0 fail=0 bad=0; loaded 3225043118 bytes
SA file size: 25262353185 bytes; state: good=1 eof=0 fail=0 bad=0
Loading SA ... done! state: good=1 eof=0 fail=0 bad=0; loaded 25262353185 bytes
Loading SAindex ... done: 1565873619 bytes
Finished loading the genome: Mon Jun  8 10:21:24 2026

Jun 08 10:21:24 ..... inserting extra sequences into genome indexes
Jun 08 10:22:15   Finished SA search, number of new SA indices = 477606
Jun 08 10:22:15   Finished qsort
Jun 08 10:22:15   Finished sorting SA indices
Jun 08 10:22:48   Finished inserting SA indices
Jun 08 10:22:48 ... generating Suffix Array index
Jun 08 10:26:42 ... completed Suffix Array index
Jun 08 10:26:42   Finished SAi
Processing splice junctions database sjdbN=386086,   pGe.sjdbOverhang=150 
To accommodate alignIntronMax=100000 redefined winBinNbits=16
To accommodate alignIntronMax=100000 and alignMatesGapMax=100000, redefined winFlankNbins=2 and winAnchorDistNbins=4
Jun 08 10:26:42   Loaded database junctions from the generated genome /tmp/nxf.6DJG8gVLqU/ctat_genome_lib_build_dir/ref_genome.fa.star.idx//sjdbList.out.tab: 386086 total junctions

Jun 08 10:26:42 ..... processing annotations GTF
Processing pGe.sjdbGTFfile=/tmp/nxf.6DJG8gVLqU/fi_workdir/tih_rna_sample_00660_B23MVV7LT4_1.gtf, found:
		111 transcripts
		731 exons (non-collapsed)
		195 collapsed junctions
Total junctions: 386281
Jun 08 10:26:42 ..... finished GTF processing

Jun 08 10:26:42   Finished preparing junctions
Jun 08 10:26:42 ..... inserting junctions into the genome indices
Jun 08 10:26:44   Finished SA search: number of new junctions=195, old junctions=386086
Jun 08 10:26:44   Finished sorting SA indicesL nInd=117000
Genome size with junctions=3225626101  3109355520   116270581
GstrandBit1=32   GstrandBit=32
Jun 08 10:27:39   Finished inserting junction indices
Jun 08 10:27:43   Finished SAi
Jun 08 10:27:43 ..... finished inserting junctions into genome
Created thread # 1
Created thread # 2
Created thread # 3
Created thread # 4
Created thread # 5
Created thread # 6
Created thread # 7
Starting to map file # 0
mate 1:   /tmp/nxf.6DJG8gVLqU/tih_rna_sample_00660_B23MVV7LT4_1_1.fastp.fastq.gz
mate 2:   /tmp/nxf.6DJG8gVLqU/tih_rna_sample_00660_B23MVV7LT4_1_2.fastp.fastq.gz
Thread #0 end of input stream, nextChar=-1
BAM sorting: 31958 mapped reads
BAM sorting bins genomic start loci:
1	194	3195
2	194	6283
3	194	8247
4	194	12923
5	194	16476
6	194	34136
7	194	41207
8	194	47317
9	194	48612
10	194	50838
11	194	55530
12	194	57417
13	195	3714
14	196	1424
15	196	11511
16	197	2396
17	197	11042
18	198	1740
19	198	1849
20	198	4248
21	198	4700
22	198	5849
23	198	7630
24	198	8772
25	198	18419
26	198	18508
27	198	19292
28	198	19985
29	198	21667
30	198	24985
31	198	27068
32	198	28131
33	198	28778
34	199	9327
35	199	13480
36	199	14648
37	199	17052
38	199	19947
39	199	29019
40	199	41301
41	200	1616
42	201	1071
43	201	3444
44	201	5640
45	201	7869
46	201	7973
47	201	10422
48	201	15077
Completed: thread #0
Completed: thread #2
Completed: thread #1
Joined thread # 1
Joined thread # 2
Completed: thread #6
Completed: thread #5
Completed: thread #4
Completed: thread #3
Joined thread # 3
Joined thread # 4
Joined thread # 5
Joined thread # 6
Completed: thread #7
Joined thread # 7
Jun 08 11:46:55 ..... started sorting BAM
Max memory needed for sorting = 2149734
ALL DONE!