[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00591_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (15809751) splicing reads. 277652 splicing junctions. 181295 known splicing junctions. 96357 novel splicing junctions.
sampling 10% (31619502) splicing reads. 349172 splicing junctions. 194112 known splicing junctions. 155060 novel splicing junctions.
sampling 15% (47429253) splicing reads. 399621 splicing junctions. 200785 known splicing junctions. 198836 novel splicing junctions.
sampling 20% (63239005) splicing reads. 438725 splicing junctions. 205150 known splicing junctions. 233575 novel splicing junctions.
sampling 25% (79048756) splicing reads. 470302 splicing junctions. 208223 known splicing junctions. 262079 novel splicing junctions.
sampling 30% (94858507) splicing reads. 496122 splicing junctions. 210486 known splicing junctions. 285636 novel splicing junctions.
sampling 35% (110668259) splicing reads. 517969 splicing junctions. 212271 known splicing junctions. 305698 novel splicing junctions.
sampling 40% (126478010) splicing reads. 536642 splicing junctions. 213738 known splicing junctions. 322904 novel splicing junctions.
sampling 45% (142287761) splicing reads. 552368 splicing junctions. 214944 known splicing junctions. 337424 novel splicing junctions.
sampling 50% (158097513) splicing reads. 565595 splicing junctions. 215898 known splicing junctions. 349697 novel splicing junctions.
sampling 55% (173907264) splicing reads. 577253 splicing junctions. 216660 known splicing junctions. 360593 novel splicing junctions.
sampling 60% (189717015) splicing reads. 587251 splicing junctions. 217345 known splicing junctions. 369906 novel splicing junctions.
sampling 65% (205526766) splicing reads. 595764 splicing junctions. 217983 known splicing junctions. 377781 novel splicing junctions.
sampling 70% (221336518) splicing reads. 603071 splicing junctions. 218507 known splicing junctions. 384564 novel splicing junctions.
sampling 75% (237146269) splicing reads. 609397 splicing junctions. 218935 known splicing junctions. 390462 novel splicing junctions.
sampling 80% (252956020) splicing reads. 614597 splicing junctions. 219280 known splicing junctions. 395317 novel splicing junctions.
sampling 85% (268765772) splicing reads. 618837 splicing junctions. 219551 known splicing junctions. 399286 novel splicing junctions.
sampling 90% (284575523) splicing reads. 622210 splicing junctions. 219756 known splicing junctions. 402454 novel splicing junctions.
sampling 95% (300385274) splicing reads. 624738 splicing junctions. 219908 known splicing junctions. 404830 novel splicing junctions.
sampling 100% (316195026) splicing reads. 626393 splicing junctions. 220011 known splicing junctions. 406382 novel splicing junctions.