File Info

Filename
.command.log
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ea/51178794d9e504162fcc7282988216/.command.log
Size
40.5 KB
Attempt
  Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/77/e1f3a1712b5090e567b0d600f63e33/tih_rna_sample_00588_B23MVV7LT4_1.SJ.out.tab
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ea/51178794d9e504162fcc7282988216/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/77/e1f3a1712b5090e567b0d600f63e33/tih_rna_sample_00588_B23MVV7LT4_1.Chimeric.out.junction
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/77/e1f3a1712b5090e567b0d600f63e33/tih_rna_sample_00588_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ea/51178794d9e504162fcc7282988216/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1c/2b18b9be298aa465f59ec7788ec288/tih_rna_sample_00588_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
==> STAGING COMPLETE (7 inputs)

08:53:04 : INFO : -reading targets list: ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
-mapping reads to genes

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[11160000], rate=20925000.00/min 
[11170000], rate=20943750.00/min 
[11180000], rate=20962500.00/min 
[11190000], rate=20981250.00/min 
[11200000], rate=21000000.00/min 
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[11220000], rate=20400000.00/min 
[11230000], rate=20418181.82/min 
[11240000], rate=20436363.64/min 
[11250000], rate=20454545.45/min 
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[11290000], rate=20527272.73/min 08:53:46 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file tih_rna_sample_00588_B23MVV7LT4_1.introns  --ctat_genome_lib ctat_genome_lib_build_dir  --intron_col 0  > tih_rna_sample_00588_B23MVV7LT4_1.cancer.introns.prelim 
Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18.
-tih_rna_sample_00588_B23MVV7LT4_1.introns: identified 7207 cancer introns
08:53:46 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file tih_rna_sample_00588_B23MVV7LT4_1.introns  --ctat_genome_lib ctat_genome_lib_build_dir  --intron_col 0  > tih_rna_sample_00588_B23MVV7LT4_1.cancer.introns.prelim 
08:53:46 : INFO : Running: touch /tmp/nxf.AUPBSbDgvc/tih_rna_sample_00588_B23MVV7LT4_1.chckpts/prelim_introns.ok
08:53:47 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates tih_rna_sample_00588_B23MVV7LT4_1.cancer.introns.prelim --min_total_reads 5  > tih_rna_sample_00588_B23MVV7LT4_1.cancer.introns 
08:53:47 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates tih_rna_sample_00588_B23MVV7LT4_1.cancer.introns.prelim --min_total_reads 5  > tih_rna_sample_00588_B23MVV7LT4_1.cancer.introns 
08:53:47 : INFO : Running: touch /tmp/nxf.AUPBSbDgvc/tih_rna_sample_00588_B23MVV7LT4_1.chckpts/introns_filtered.ok
08:53:47 : INFO : -found 2817 cancer introns
08:53:47 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns tih_rna_sample_00588_B23MVV7LT4_1.introns  --cancer_introns tih_rna_sample_00588_B23MVV7LT4_1.cancer.introns  --genome_lib_dir ctat_genome_lib_build_dir  --output_bed tih_rna_sample_00588_B23MVV7LT4_1.introns.for_IGV.bed 
08:53:47 : INFO :  Creating the BED File.
08:53:52 : INFO : Saving Bed File as tih_rna_sample_00588_B23MVV7LT4_1.introns.for_IGV.bed
08:53:52 : INFO : Execution Time = 0.09 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns tih_rna_sample_00588_B23MVV7LT4_1.introns  --cancer_introns tih_rna_sample_00588_B23MVV7LT4_1.cancer.introns  --genome_lib_dir ctat_genome_lib_build_dir  --output_bed tih_rna_sample_00588_B23MVV7LT4_1.introns.for_IGV.bed 
08:53:52 : INFO : Running: touch /tmp/nxf.AUPBSbDgvc/tih_rna_sample_00588_B23MVV7LT4_1.chckpts/intron_igv_bed.ok
08:53:52 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed tih_rna_sample_00588_B23MVV7LT4_1.introns.for_IGV.bed  --bam tih_rna_sample_00588_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam  --output_prefix tih_rna_sample_00588_B23MVV7LT4_1 
[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00588_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
09:10:16 : INFO : Execution Time = 16.40 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed tih_rna_sample_00588_B23MVV7LT4_1.introns.for_IGV.bed  --bam tih_rna_sample_00588_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam  --output_prefix tih_rna_sample_00588_B23MVV7LT4_1 
09:10:16 : INFO : Running: touch /tmp/nxf.AUPBSbDgvc/tih_rna_sample_00588_B23MVV7LT4_1.chckpts/reads_alignments_extracted.ok
09:10:16 : INFO : Running: samtools sort -o tih_rna_sample_00588_B23MVV7LT4_1.cancer_intron_reads.sorted.bam tih_rna_sample_00588_B23MVV7LT4_1.cancer_intron_reads.bam
09:10:17 : INFO : Execution Time = 0.01 minutes. CMD: samtools sort -o tih_rna_sample_00588_B23MVV7LT4_1.cancer_intron_reads.sorted.bam tih_rna_sample_00588_B23MVV7LT4_1.cancer_intron_reads.bam
09:10:17 : INFO : Running: touch /tmp/nxf.AUPBSbDgvc/tih_rna_sample_00588_B23MVV7LT4_1.chckpts/sort_cancer_intron_reads.ok
09:10:17 : INFO : Running: samtools sort -o tih_rna_sample_00588_B23MVV7LT4_1.gene_reads.sorted.bam tih_rna_sample_00588_B23MVV7LT4_1.gene_reads.bam