Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/bf/50e1ba5bc109e3d0919a8d6f5345fd/tih_rna_sample_00725_B23MVV7LT4_1_2.fastp.fastq.gz
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/65/916c0d0beee9ff63af3c4ad9314b51/.command.sh
Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/65/916c0d0beee9ff63af3c4ad9314b51/.command.run
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/bf/50e1ba5bc109e3d0919a8d6f5345fd/tih_rna_sample_00725_B23MVV7LT4_1_1.fastp.fastq.gz
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9d/4a698173a932b6cdfc0916257440fe/tih_rna_sample_00725_B23MVV7LT4_1.fusionreport.tsv
==> STAGING COMPLETE (6 inputs)
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_pair_to_mini_genome_join.pl --fusions tih_rna_sample_00725_B23MVV7LT4_1.fusionreport.tsv --gtf /tmp/nxf.Akm1c8xPJn/ctat_genome_lib_build_dir/ref_annot.gtf --genome_fa /tmp/nxf.Akm1c8xPJn/ctat_genome_lib_build_dir/ref_genome.fa --out_prefix /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1 --shrink_introns --max_intron_length 1000
Done.
Execution Time = 0.12 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_pair_to_mini_genome_join.pl --fusions tih_rna_sample_00725_B23MVV7LT4_1.fusionreport.tsv --gtf /tmp/nxf.Akm1c8xPJn/ctat_genome_lib_build_dir/ref_annot.gtf --genome_fa /tmp/nxf.Akm1c8xPJn/ctat_genome_lib_build_dir/ref_genome.fa --out_prefix /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1 --shrink_introns --max_intron_length 1000
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/fusion_contigs.ok
Running: cp /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.fa /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fa
Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.fa /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fa
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/cp_contigs_file_workdir
Running: cp /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.gtf /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.gtf
Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.gtf /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.gtf
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/cp_gtf_file_workdir.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fasta_and_gtf_to_cytoband.pl /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.fa /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.gtf > /tmp/nxf.Akm1c8xPJn/cytoBand.txt
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fasta_and_gtf_to_cytoband.pl /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.fa /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.gtf > /tmp/nxf.Akm1c8xPJn/cytoBand.txt
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/cytoband.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/gtf_gene_to_bed.pl /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.gtf > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.bed
-parsing GTF file: /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.gtf
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/gtf_gene_to_bed.pl /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.gtf > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.bed
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/merged_contig_gtf_to_bed.ok
Running: sort -k1,1 -k2,2n /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.bed > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.bed.sorted.bed
Execution Time = 0.00 minutes. CMD: sort -k1,1 -k2,2n /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.bed > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.bed.sorted.bed
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/tih_rna_sample_00725_B23MVV7LT4_1.bed.bedsort.ok
Running: bgzip -f /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.bed.sorted.bed
Execution Time = 0.00 minutes. CMD: bgzip -f /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.bed.sorted.bed
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/tih_rna_sample_00725_B23MVV7LT4_1.bed.bgzip.ok
Running: tabix -p bed /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.bed.sorted.bed.gz
Execution Time = 0.00 minutes. CMD: tabix -p bed /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.bed.sorted.bed.gz
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/tih_rna_sample_00725_B23MVV7LT4_1.bed.tabix.ok
Running: samtools faidx /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.fa
Execution Time = 0.00 minutes. CMD: samtools faidx /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.fa
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/merged_contig_fai.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/run_FI_STAR.pl --genome /tmp/nxf.Akm1c8xPJn/ctat_genome_lib_build_dir/ref_genome.fa --patch /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fa --max_mate_dist 100000 -G /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.gtf --CPU 8 --out_prefix tih_rna_sample_00725_B23MVV7LT4_1.star --out_dir /tmp/nxf.Akm1c8xPJn/fi_workdir --reads "/tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1_1.fastp.fastq.gz /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1_2.fastp.fastq.gz"
* Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.Akm1c8xPJn/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1_1.fastp.fastq.gz /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c'
Jun 08 10:48:59 ..... started STAR run
Jun 08 10:48:59 ..... loading genome
Jun 08 10:50:41 ... generating Suffix Array index
Jun 08 10:54:24 ... completed Suffix Array index
Jun 08 10:54:25 ..... processing annotations GTF
Jun 08 10:54:25 ..... inserting junctions into the genome indices
Jun 08 10:55:25 ..... started mapping
Jun 08 11:51:00 ..... finished mapping
Jun 08 11:51:02 ..... started sorting BAM
Jun 08 11:51:10 ..... finished successfully
* Running CMD: mv Aligned.sortedByCoord.out.bam tih_rna_sample_00725_B23MVV7LT4_1.star.sortedByCoord.out.bam
* Running CMD: samtools index tih_rna_sample_00725_B23MVV7LT4_1.star.sortedByCoord.out.bam
Execution Time = 62.45 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/run_FI_STAR.pl --genome /tmp/nxf.Akm1c8xPJn/ctat_genome_lib_build_dir/ref_genome.fa --patch /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fa --max_mate_dist 100000 -G /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.gtf --CPU 8 --out_prefix tih_rna_sample_00725_B23MVV7LT4_1.star --out_dir /tmp/nxf.Akm1c8xPJn/fi_workdir --reads "/tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1_1.fastp.fastq.gz /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1_2.fastp.fastq.gz"
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/run_STAR.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/bam_mark_duplicates.py -i /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.sortedByCoord.out.bam -o /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam --remove_dups
11:52:48 : INFO : Done.
Execution Time = 1.37 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/bam_mark_duplicates.py -i /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.sortedByCoord.out.bam -o /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam --remove_dups
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/mark_dup_reads.ok
Running: samtools index /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam
Execution Time = 0.16 minutes. CMD: samtools index /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/mark_dups_reads.index.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/get_fusion_JUNCTION_reads_from_fusion_contig_bam.pl --gtf_file /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.gtf --MIN_ALIGN_PER_ID 96 --bam /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam --genome_lib_dir ctat_genome_lib_build_dir > /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam
-parsing GTF file: /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.gtf
-parsing /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam
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[17080000] -done parsing /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam. Extracting junction info.
junction read elimination tally: $VAR1 = {
'num_hits: 7 != num_counted_on_fusion_contigs 3 ' => 12,
'num_hits: 6 != num_counted_on_fusion_contigs 3 ' => 33,
'num_hits: 7 != num_counted_on_fusion_contigs 5 ' => 15,
'low complexity anchor region' => 1,
'per_id < 96' => 202085,
'num_hits: 4 != num_counted_on_fusion_contigs 3 ' => 32316,
'num_hits: 6 != num_counted_on_fusion_contigs 5 ' => 315,
'num_hits: 4 != num_counted_on_fusion_contigs 2 ' => 14264,
'num_hits: 3 != num_counted_on_fusion_contigs 2 ' => 81910,
'num_hits: 5 != num_counted_on_fusion_contigs 3 ' => 19416,
' ** passed ** ' => 1288,
'num_hits: 9 != num_counted_on_fusion_contigs 4 ' => 4,
'num_hits: 4 != num_counted_on_fusion_contigs 1 ' => 1510,
'num_hits: 5 != num_counted_on_fusion_contigs 4 ' => 7976,
'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 163659,
'num_hits: 7 != num_counted_on_fusion_contigs 6 ' => 114,
'num_hits: 5 != num_counted_on_fusion_contigs 1 ' => 2140,
'num_hits: 7 != num_counted_on_fusion_contigs 1 ' => 2,
'excessive soft clipping' => 501659,
'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 13062,
'num_hits: 6 != num_counted_on_fusion_contigs 1 ' => 5,
'num_hits: 9 != num_counted_on_fusion_contigs 6 ' => 18,
'seq-similar region overlap' => 47,
'num genes matched < 2' => 8,
'exons hit < 2' => 7,
'num_hits: 7 != num_counted_on_fusion_contigs 2 ' => 12,
'num_hits: 6 != num_counted_on_fusion_contigs 4 ' => 108,
'num_hits: 7 != num_counted_on_fusion_contigs 4 ' => 16,
'num_hits: 6 != num_counted_on_fusion_contigs 2 ' => 26,
'num_hits: 5 != num_counted_on_fusion_contigs 2 ' => 2046
};
-writing fusion junction support info.
Execution Time = 8.89 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/get_fusion_JUNCTION_reads_from_fusion_contig_bam.pl --gtf_file /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.gtf --MIN_ALIGN_PER_ID 96 --bam /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam --genome_lib_dir ctat_genome_lib_build_dir > /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/get_fusion_SPANNING_reads_from_bam.from_chim_summary.pl --gtf_file /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.gtf --MIN_ALIGN_PER_ID 96 --bam /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam --junction_info /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_junction_info --genome_lib_dir ctat_genome_lib_build_dir > /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_span_reads.sam
-outputting the spanning read info: /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info.
- counting read alignments among fusion contigs.
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[16965000] -fusion SPANNING read extraction for scaff: TNFAIP3--IGH@-ext
[1000]
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[14000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4
-filtered reads reasons: $VAR1 = {
'lacks exon overlap' => 511,
'seq similar region alignment' => 5414
};
Execution Time = 18.81 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/get_fusion_SPANNING_reads_from_bam.from_chim_summary.pl --gtf_file /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.gtf --MIN_ALIGN_PER_ID 96 --bam /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam --junction_info /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_junction_info --genome_lib_dir ctat_genome_lib_build_dir > /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_span_reads.sam
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/coalesce_junction_and_spanning_info.pl /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_junction_info /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info 1 > /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/coalesce_junction_and_spanning_info.pl /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_junction_info /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info 1 > /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/coalesce_junc_n_span.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_EM_runner.pl /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary > /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj
EM: Starting log likelihood: -1407.169529
EM: Round [1] log likelihood: -1406.151548
EM: Round [2] log likelihood: -1406.130852
EM: Round [3] log likelihood: -1406.123057
EM: Round [4] log likelihood: -1406.115538
EM: Round [5] log likelihood: -1406.108200
EM: Round [6] log likelihood: -1406.101037
EM: Round [7] log likelihood: -1406.094043
EM: Round [8] log likelihood: -1406.087212
EM: Round [9] log likelihood: -1406.080541
EM: Round [10] log likelihood: -1406.074024
EM: Round [11] log likelihood: -1406.067657
EM: Round [12] log likelihood: -1406.061435
EM: Round [13] log likelihood: -1406.055354
EM: Round [14] log likelihood: -1406.049410
EM: Round [15] log likelihood: -1406.043600
EM: Round [16] log likelihood: -1406.037920
EM: Round [17] log likelihood: -1406.032365
EM: Round [18] log likelihood: -1406.026933
EM: Round [19] log likelihood: -1406.021620
EM: Round [20] log likelihood: -1406.016423
EM: Round [21] log likelihood: -1406.011339
EM: Round [22] log likelihood: -1406.006364
EM: Round [23] log likelihood: -1406.001497
EM: Round [24] log likelihood: -1405.996733
EM: Round [25] log likelihood: -1405.992071
EM: Round [26] log likelihood: -1405.987508
EM: Round [27] log likelihood: -1405.983041
EM: Round [28] log likelihood: -1405.978668
EM: Round [29] log likelihood: -1405.974386
EM: Round [30] log likelihood: -1405.970194
EM: Round [31] log likelihood: -1405.966088
EM: Round [32] log likelihood: -1405.962068
EM: Round [33] log likelihood: -1405.958130
EM: Round [34] log likelihood: -1405.954272
EM: Round [35] log likelihood: -1405.950494
EM: Round [36] log likelihood: -1405.946792
EM: Round [37] log likelihood: -1405.943165
EM: Round [38] log likelihood: -1405.939612
EM: Round [39] log likelihood: -1405.936130
EM: Round [40] log likelihood: -1405.932718
EM: Round [41] log likelihood: -1405.929374
EM: Round [42] log likelihood: -1405.926096
EM: Round [43] log likelihood: -1405.922884
EM: Round [44] log likelihood: -1405.919735
EM: Round [45] log likelihood: -1405.916649
EM: Round [46] log likelihood: -1405.913623
EM: Round [47] log likelihood: -1405.910656
EM: Round [48] log likelihood: -1405.907747
EM: Round [49] log likelihood: -1405.904895
EM: Round [50] log likelihood: -1405.902099
EM: Round [51] log likelihood: -1405.899357
EM: Round [52] log likelihood: -1405.896667
EM: Round [53] log likelihood: -1405.894030
EM: Round [54] log likelihood: -1405.891443
EM: Round [55] log likelihood: -1405.888906
EM: Round [56] log likelihood: -1405.886418
EM: Round [57] log likelihood: -1405.883977
EM: Round [58] log likelihood: -1405.881582
EM: Round [59] log likelihood: -1405.879233
EM: Round [60] log likelihood: -1405.876929
EM: Round [61] log likelihood: -1405.874668
EM: Round [62] log likelihood: -1405.872450
EM: Round [63] log likelihood: -1405.870273
EM: Round [64] log likelihood: -1405.868137
EM: Round [65] log likelihood: -1405.866042
EM: Round [66] log likelihood: -1405.863985
EM: Round [67] log likelihood: -1405.861967
EM: Round [68] log likelihood: -1405.859987
EM: Round [69] log likelihood: -1405.858043
EM: Round [70] log likelihood: -1405.856136
EM: Round [71] log likelihood: -1405.854264
EM: Round [72] log likelihood: -1405.852426
EM: Round [73] log likelihood: -1405.850622
EM: Round [74] log likelihood: -1405.848852
EM: Round [75] log likelihood: -1405.847114
EM: Round [76] log likelihood: -1405.845408
EM: Round [77] log likelihood: -1405.843733
EM: Round [78] log likelihood: -1405.842089
EM: Round [79] log likelihood: -1405.840475
EM: Round [80] log likelihood: -1405.838891
EM: Round [81] log likelihood: -1405.837335
EM: Round [82] log likelihood: -1405.835808
EM: Round [83] log likelihood: -1405.834308
EM: Round [84] log likelihood: -1405.832835
EM: Round [85] log likelihood: -1405.831389
EM: Round [86] log likelihood: -1405.829970
EM: Round [87] log likelihood: -1405.828576
EM: Round [88] log likelihood: -1405.827207
EM: Round [89] log likelihood: -1405.825862
EM: Round [90] log likelihood: -1405.824542
EM: Round [91] log likelihood: -1405.823246
EM: Round [92] log likelihood: -1405.821972
EM: Round [93] log likelihood: -1405.820722
EM: Round [94] log likelihood: -1405.819494
EM: Round [95] log likelihood: -1405.818288
EM: Round [96] log likelihood: -1405.817103
EM: Round [97] log likelihood: -1405.815939
EM: Round [98] log likelihood: -1405.814797
EM: Round [99] log likelihood: -1405.813674
EM: Round [100] log likelihood: -1405.812572
EM: Round [101] log likelihood: -1405.811489
EM: Round [102] log likelihood: -1405.810425
EM: Round [103] log likelihood: -1405.809380
EM: Round [104] log likelihood: -1405.808353
EM: Round [105] log likelihood: -1405.807345
EM: Round [106] log likelihood: -1405.806354
EM: Round [107] log likelihood: -1405.805381
EM: Round [108] log likelihood: -1405.804425
EM: Round [109] log likelihood: -1405.803486
EM: Round [110] log likelihood: -1405.802564
EM: Round [111] log likelihood: -1405.801657
EM: Round [112] log likelihood: -1405.800767
EM: Round [113] log likelihood: -1405.799892
EM: Round [114] log likelihood: -1405.799032
EM: Round [115] log likelihood: -1405.798188
EM: Round [116] log likelihood: -1405.797358
EM: Round [117] log likelihood: -1405.796543
EM: Round [118] log likelihood: -1405.795742
EM: Round [119] log likelihood: -1405.794955
EM: Round [120] log likelihood: -1405.794181
EM: Round [121] log likelihood: -1405.793422
EM: Round [122] log likelihood: -1405.792675
EM: Round [123] log likelihood: -1405.791941
EM: Round [124] log likelihood: -1405.791221
EM: Round [125] log likelihood: -1405.790512
EM: Round [126] log likelihood: -1405.789816
EM: Round [127] log likelihood: -1405.789132
EM: Round [128] log likelihood: -1405.788460
EM: Round [129] log likelihood: -1405.787799
EM: Round [130] log likelihood: -1405.787150
EM: Round [131] log likelihood: -1405.786513
EM: Round [132] log likelihood: -1405.785886
EM: Round [133] log likelihood: -1405.785270
EM: Round [134] log likelihood: -1405.784664
EM: Round [135] log likelihood: -1405.784070
EM: Round [136] log likelihood: -1405.783485
EM: Round [137] log likelihood: -1405.782910
EM: Round [138] log likelihood: -1405.782346
EM: Round [139] log likelihood: -1405.781791
EM: Round [140] log likelihood: -1405.781245
EM: Round [141] log likelihood: -1405.780709
EM: Round [142] log likelihood: -1405.780183
EM: Round [143] log likelihood: -1405.779665
EM: Round [144] log likelihood: -1405.779156
EM: Round [145] log likelihood: -1405.778656
EM: Round [146] log likelihood: -1405.778164
EM: Round [147] log likelihood: -1405.777681
EM: Round [148] log likelihood: -1405.777206
EM: Round [149] log likelihood: -1405.776740
EM: Round [150] log likelihood: -1405.776281
EM: Round [151] log likelihood: -1405.775830
EM: Round [152] log likelihood: -1405.775387
EM: Round [153] log likelihood: -1405.774951
EM: Round [154] log likelihood: -1405.774523
EM: Round [155] log likelihood: -1405.774103
EM: Round [156] log likelihood: -1405.773689
EM: Round [157] log likelihood: -1405.773282
EM: Round [158] log likelihood: -1405.772883
EM: Round [159] log likelihood: -1405.772490
EM: Round [160] log likelihood: -1405.772104
EM: Round [161] log likelihood: -1405.771724
EM: Round [162] log likelihood: -1405.771351
EM: Round [163] log likelihood: -1405.770984
EM: Round [164] log likelihood: -1405.770624
EM: Round [165] log likelihood: -1405.770270
EM: Round [166] log likelihood: -1405.769921
EM: Round [167] log likelihood: -1405.769579
EM: Round [168] log likelihood: -1405.769242
EM: Round [169] log likelihood: -1405.768912
EM: Round [170] log likelihood: -1405.768586
EM: Round [171] log likelihood: -1405.768267
EM: Round [172] log likelihood: -1405.767952
EM: Round [173] log likelihood: -1405.767643
EM: Round [174] log likelihood: -1405.767340
EM: Round [175] log likelihood: -1405.767041
EM: Round [176] log likelihood: -1405.766748
EM: Round [177] log likelihood: -1405.766459
EM: Round [178] log likelihood: -1405.766176
EM: Round [179] log likelihood: -1405.765897
EM: Round [180] log likelihood: -1405.765623
EM: Round [181] log likelihood: -1405.765353
EM: Round [182] log likelihood: -1405.765088
EM: Round [183] log likelihood: -1405.764828
EM: Round [184] log likelihood: -1405.764572
EM: Round [185] log likelihood: -1405.764320
EM: Round [186] log likelihood: -1405.764073
EM: Round [187] log likelihood: -1405.763830
EM: Round [188] log likelihood: -1405.763591
EM: Round [189] log likelihood: -1405.763356
EM: Round [190] log likelihood: -1405.763124
EM: Round [191] log likelihood: -1405.762897
EM: Round [192] log likelihood: -1405.762674
EM: Round [193] log likelihood: -1405.762454
EM: Round [194] log likelihood: -1405.762238
EM: Round [195] log likelihood: -1405.762026
EM: Round [196] log likelihood: -1405.761817
EM: Round [197] log likelihood: -1405.761612
EM: Round [198] log likelihood: -1405.761411
EM: Round [199] log likelihood: -1405.761212
EM: Round [200] log likelihood: -1405.761017
EM: Round [201] log likelihood: -1405.760826
EM: Round [202] log likelihood: -1405.760637
EM: Round [203] log likelihood: -1405.760452
EM: Round [204] log likelihood: -1405.760270
EM: Round [205] log likelihood: -1405.760091
EM: Round [206] log likelihood: -1405.759915
EM: Round [207] log likelihood: -1405.759742
EM: Round [208] log likelihood: -1405.759572
EM: Round [209] log likelihood: -1405.759404
EM: Round [210] log likelihood: -1405.759240
EM: Round [211] log likelihood: -1405.759078
EM: Round [212] log likelihood: -1405.758919
EM: Round [213] log likelihood: -1405.758763
EM: Round [214] log likelihood: -1405.758609
EM: Round [215] log likelihood: -1405.758458
EM: Round [216] log likelihood: -1405.758310
EM: Round [217] log likelihood: -1405.758163
EM: Round [218] log likelihood: -1405.758020
EM: Round [219] log likelihood: -1405.757879
EM: Round [220] log likelihood: -1405.757740
EM: Round [221] log likelihood: -1405.757603
EM: Round [222] log likelihood: -1405.757469
EM: Round [223] log likelihood: -1405.757337
EM: Round [224] log likelihood: -1405.757207
EM: Round [225] log likelihood: -1405.757080
EM: Round [226] log likelihood: -1405.756954
EM: Round [227] log likelihood: -1405.756831
EM: Round [228] log likelihood: -1405.756710
EM: Round [229] log likelihood: -1405.756591
EM: Round [230] log likelihood: -1405.756474
EM: Round [231] log likelihood: -1405.756358
EM: Round [232] log likelihood: -1405.756245
EM: Round [233] log likelihood: -1405.756134
EM: Round [234] log likelihood: -1405.756024
EM: Round [235] log likelihood: -1405.755916
EM: Round [236] log likelihood: -1405.755811
EM: Round [237] log likelihood: -1405.755706
EM: Round [238] log likelihood: -1405.755604
EM: Round [239] log likelihood: -1405.755503
EM: Round [240] log likelihood: -1405.755404
EM: Stopping iterations at round 240 due to insufficient improvement in likelihood.
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_EM_runner.pl /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary > /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/init_EM_adj_counts.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/filter_fusions_by_frag_thresholds.pl --min_junction_reads 0 --min_sum_frags 1 --min_novel_junction_support 3 --min_spanning_frags_only 5 --fusion_preds /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj --require_LDAS 1 > /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/filter_fusions_by_frag_thresholds.pl --min_junction_reads 0 --min_sum_frags 1 --min_novel_junction_support 3 --min_spanning_frags_only 5 --fusion_preds /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj --require_LDAS 1 > /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/filter_by_frag_threshs.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/column_extractions.pl /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary LeftGene,RightGene,JunctionReads > /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.fusion_junction_read_accs
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/column_extractions.pl /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary LeftGene,RightGene,JunctionReads > /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.fusion_junction_read_accs
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/prep_igv_extract_junc_reads.ok
Running: bash -c "set -eof pipefail; /usr/local/src/STAR-Fusion/FusionInspector/util/retrieve_fusion_junction_reads_by_accession.pl /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.fusion_junction_read_accs /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam | samtools view -@ 8 -bT /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.fa - | samtools sort -@ 8 - -o /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.junction_reads.bam"
[bam_sort_core] merging from 0 files and 8 in-memory blocks...
Execution Time = 0.00 minutes. CMD: bash -c "set -eof pipefail; /usr/local/src/STAR-Fusion/FusionInspector/util/retrieve_fusion_junction_reads_by_accession.pl /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.fusion_junction_read_accs /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam | samtools view -@ 8 -bT /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.fa - | samtools sort -@ 8 - -o /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.junction_reads.bam"
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/prep_igv_junc_reads_bam.ok
Running: samtools index /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.junction_reads.bam
Execution Time = 0.00 minutes. CMD: samtools index /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.junction_reads.bam
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/samtools_idx_junc_reads_bam.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_pair_to_bed.pl /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.junction_reads.bam > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.junction_reads.bam.bed
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_pair_to_bed.pl /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.junction_reads.bam > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.junction_reads.bam.bed
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/tih_rna_sample_00725_B23MVV7LT4_1.junction_reads.bam.bam_to_bed.ok
Running: sort -k1,1 -k2,2n /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.junction_reads.bam.bed > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.junction_reads.bam.bed.sorted.bed
Execution Time = 0.00 minutes. CMD: sort -k1,1 -k2,2n /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.junction_reads.bam.bed > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.junction_reads.bam.bed.sorted.bed
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/tih_rna_sample_00725_B23MVV7LT4_1.junction_reads.bam.bedsort.ok
Running: bgzip -f /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.junction_reads.bam.bed.sorted.bed
Execution Time = 0.00 minutes. CMD: bgzip -f /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.junction_reads.bam.bed.sorted.bed
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/tih_rna_sample_00725_B23MVV7LT4_1.junction_reads.bam.bgzip.ok
Running: tabix -p bed /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.junction_reads.bam.bed.sorted.bed.gz
Execution Time = 0.00 minutes. CMD: tabix -p bed /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.junction_reads.bam.bed.sorted.bed.gz
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/tih_rna_sample_00725_B23MVV7LT4_1.junction_reads.bam.tabix.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/column_extractions.pl /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary LeftGene,RightGene,SpanningFrags > /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.fusion_spanning_read_accs
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/column_extractions.pl /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary LeftGene,RightGene,SpanningFrags > /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.fusion_spanning_read_accs
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/span_reads_acc.ok
Running: samtools view -H /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam -b -o /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam
Execution Time = 0.00 minutes. CMD: samtools view -H /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam -b -o /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/init_spanning_reads_bam.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/retrieve_fusion_spanning_reads_by_accession.pl /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.fusion_spanning_read_accs /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_span_reads.sam | samtools view -@ 8 -bT /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.fa - | samtools sort -@ 8 - -o /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam || :
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/retrieve_fusion_spanning_reads_by_accession.pl /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.fusion_spanning_read_accs /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_span_reads.sam | samtools view -@ 8 -bT /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.fa - | samtools sort -@ 8 - -o /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam || :
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/prep_spanning_reads.ok
Running: samtools index /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam
Execution Time = 0.00 minutes. CMD: samtools index /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/samtools_index_span_reads_bam.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_pair_to_bed.pl /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam.bed
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_pair_to_bed.pl /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam.bed
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam.bam_to_bed.ok
Running: sort -k1,1 -k2,2n /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam.bed > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam.bed.sorted.bed
Execution Time = 0.00 minutes. CMD: sort -k1,1 -k2,2n /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam.bed > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam.bed.sorted.bed
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam.bedsort.ok
Running: bgzip -f /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam.bed.sorted.bed
Execution Time = 0.00 minutes. CMD: bgzip -f /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam.bed.sorted.bed
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam.bgzip.ok
Running: tabix -p bed /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam.bed.sorted.bed.gz
Execution Time = 0.00 minutes. CMD: tabix -p bed /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam.bed.sorted.bed.gz
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/tih_rna_sample_00725_B23MVV7LT4_1.spanning_reads.bam.tabix.ok
Running: cp /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.consolidated.bam
Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.star.cSorted.dupsMarked.bam /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.consolidated.bam
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/cp_consol_bam.ok
Running: samtools index /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.consolidated.bam
Execution Time = 0.16 minutes. CMD: samtools index /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.consolidated.bam
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/index_consol_bam.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_to_frag_coords.pl --sam /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.consolidated.bam --max_insert_size 10000000
-extracting read coordinates from /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.consolidated.bam into /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.consolidated.bam.read_coords
CMD: touch /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.consolidated.bam.read_coords.ok
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.consolidated.bam.read_coords > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.consolidated.bam.read_coords.sort_by_readname
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.consolidated.bam.frag_coords > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.consolidated.bam.frag_coords.coord_sorted
Execution Time = 4.60 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_to_frag_coords.pl --sam /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.consolidated.bam --max_insert_size 10000000
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/prep_igv_sam_frag_coordss.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_summary_to_igv_JuncSpan_fmt.pl /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.consolidated.bam.frag_coords > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.igv.FusionJuncSpan
Execution Time = 0.08 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_summary_to_igv_JuncSpan_fmt.pl /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.consolidated.bam.frag_coords > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.igv.FusionJuncSpan
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/prep_igv_juncspan_fmt.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/get_pfam_domain_info.pl --finspector_gtf /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.gtf --genome_lib_dir ctat_genome_lib_build_dir > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.igv.Pfam.gff3
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/get_pfam_domain_info.pl --finspector_gtf /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.gtf --genome_lib_dir ctat_genome_lib_build_dir > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.igv.Pfam.gff3
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/prep_igv_pfam_gff3.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/transcript_gff3_to_bed.pl /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.igv.Pfam.gff3 > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.igv.Pfam.bed
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/transcript_gff3_to_bed.pl /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.igv.Pfam.gff3 > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.igv.Pfam.bed
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/prep_igv_pfam_bed.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/get_seq_similar_region_FI_coordinates.pl --finspector_gtf /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.gtf --genome_lib_dir ctat_genome_lib_build_dir > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.igv.seqsimilar.gff3
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/get_seq_similar_region_FI_coordinates.pl --finspector_gtf /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.gtf --genome_lib_dir ctat_genome_lib_build_dir > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.igv.seqsimilar.gff3
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/prep_igv_seqsim_gff3.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/transcript_gff3_to_bed.pl /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.igv.seqsimilar.gff3 > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.igv.seqsimilar.bed
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/transcript_gff3_to_bed.pl /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.igv.seqsimilar.gff3 > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.igv.seqsimilar.bed
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/prep_igv_seqsim_bed.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/append_breakpoint_junction_info_via_FI_contigs.pl /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.fa > /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/append_breakpoint_junction_info_via_FI_contigs.pl /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.fa > /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/add_splice_info.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/../FusionFilter/blast_and_promiscuity_filter.pl --fusion_preds /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --out_prefix /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1 --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50
CMD: cp /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.post_blast_and_promiscuity_filter
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/../FusionFilter/blast_and_promiscuity_filter.pl --fusion_preds /tmp/nxf.Akm1c8xPJn/fi_workdir/tih_rna_sample_00725_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --out_prefix /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1 --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/blast_filter.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/../FusionFilter/util/filter_by_annotation_rules.pl --fusions /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.post_blast_and_promiscuity_filter --genome_lib_dir /tmp/nxf.Akm1c8xPJn/ctat_genome_lib_build_dir
-done, see /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/../FusionFilter/util/filter_by_annotation_rules.pl --fusions /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.post_blast_and_promiscuity_filter --genome_lib_dir /tmp/nxf.Akm1c8xPJn/ctat_genome_lib_build_dir
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/annot_filter.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_EM_runner.pl /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj
EM: Starting log likelihood: -1163.157968
EM: Round [1] log likelihood: -1162.148081
EM: Round [2] log likelihood: -1162.135280
EM: Round [3] log likelihood: -1162.135189
EM: Stopping iterations at round 3 due to insufficient improvement in likelihood.
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_EM_runner.pl /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/EM_adj_counts.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/incorporate_FFPM_into_final_report.pl /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1_1.fastp.fastq.gz /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM
-total frags in /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1_1.fastp.fastq.gz: 295990243
Execution Time = 5.66 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/incorporate_FFPM_into_final_report.pl /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1_1.fastp.fastq.gz /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/add_FFPM.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/misc/find_microhomologies_by_kmer_matches.pl --fasta /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.fa --gtf /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.gtf > /tmp/nxf.Akm1c8xPJn/fi_workdir/microH.dat
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/misc/find_microhomologies_by_kmer_matches.pl --fasta /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.fa --gtf /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.gtf > /tmp/nxf.Akm1c8xPJn/fi_workdir/microH.dat
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/microH.dat.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/misc/append_microH_distance.py /tmp/nxf.Akm1c8xPJn/fi_workdir/microH.dat /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM.wMicroH
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/misc/append_microH_distance.py /tmp/nxf.Akm1c8xPJn/fi_workdir/microH.dat /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM.wMicroH
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/append_microH_info.ok
Running: cp /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM.wMicroH /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.FusionInspector.fusions.tsv
Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM.wMicroH /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.FusionInspector.fusions.tsv
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/cp_final.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/column_exclusions.pl /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.FusionInspector.fusions.tsv JunctionReads,SpanningFrags,CounterFusionLeftReads,CounterFusionRightReads > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.FusionInspector.fusions.abridged.tsv
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/column_exclusions.pl /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.FusionInspector.fusions.tsv JunctionReads,SpanningFrags,CounterFusionLeftReads,CounterFusionRightReads > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.FusionInspector.fusions.abridged.tsv
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/final.abridged.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/create_fusion_inspector_igvjs.py --fusion_inspector_directory /tmp/nxf.Akm1c8xPJn --json_outfile /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.fusion_inspector_web.json --file_prefix tih_rna_sample_00725_B23MVV7LT4_1
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/create_fusion_inspector_igvjs.py --fusion_inspector_directory /tmp/nxf.Akm1c8xPJn --json_outfile /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.fusion_inspector_web.json --file_prefix tih_rna_sample_00725_B23MVV7LT4_1
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/create_fi_igvjs.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/fusion-reports/create_fusion_report.py --html_template /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_report_html_template/igvjs_fusion.html --fusions_json /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.fusion_inspector_web.json --input_file_prefix tih_rna_sample_00725_B23MVV7LT4_1 --html_output /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.fusion_inspector_web.html
Warning - not locating file: /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.gmap_trinity_GG.fusions.gff3.bed
Execution Time = 0.02 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/fusion-reports/create_fusion_report.py --html_template /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_report_html_template/igvjs_fusion.html --fusions_json /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.fusion_inspector_web.json --input_file_prefix tih_rna_sample_00725_B23MVV7LT4_1 --html_output /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.fusion_inspector_web.html
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/fusion_reports_html.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/FusionAnnotator/FusionAnnotator --annotate /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.FusionInspector.fusions.abridged.tsv --genome_lib_dir /tmp/nxf.Akm1c8xPJn/ctat_genome_lib_build_dir > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.FusionInspector.fusions.abridged.tsv.annotated
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionAnnotator/FusionAnnotator --annotate /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.FusionInspector.fusions.abridged.tsv --genome_lib_dir /tmp/nxf.Akm1c8xPJn/ctat_genome_lib_build_dir > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.FusionInspector.fusions.abridged.tsv.annotated
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/fusion_annotator.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/FusionAnnotator/util/fusion_to_coding_region_effect.pl --fusions /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.FusionInspector.fusions.abridged.tsv.annotated --genome_lib_dir /tmp/nxf.Akm1c8xPJn/ctat_genome_lib_build_dir > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.FusionInspector.fusions.abridged.tsv.annotated.coding_effect
WARNING, no entry stored in dbm for [ENSG00000236438.7]
WARNING, no entry stored in dbm for [ENSG00000282057.1]
WARNING, no entry stored in dbm for [ENSG00000282057.1]
WARNING, no entry stored in dbm for [ENSG00000282057.1]
WARNING, no entry stored in dbm for [ENSG00000226049.3]
WARNING, no entry stored in dbm for [ENSG00000260404.3]
WARNING, no entry stored in dbm for [ENSG00000272578.5]
WARNING, no entry stored in dbm for [ENSG00000272578.5]
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionAnnotator/util/fusion_to_coding_region_effect.pl --fusions /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.FusionInspector.fusions.abridged.tsv.annotated --genome_lib_dir /tmp/nxf.Akm1c8xPJn/ctat_genome_lib_build_dir > /tmp/nxf.Akm1c8xPJn/tih_rna_sample_00725_B23MVV7LT4_1.FusionInspector.fusions.abridged.tsv.annotated.coding_effect
Running: touch /tmp/nxf.Akm1c8xPJn/chckpts_dir/fusion_coding_region_effect.ok
ls: cannot access 'IGV_inputs': No such file or directory