Done. -parsing GTF file: /tmp/nxf.K3w4JnSYAS/tih_rna_sample_00636_B23MVV7LT4_1.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.K3w4JnSYAS/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.K3w4JnSYAS/tih_rna_sample_00636_B23MVV7LT4_1_1.fastp.fastq.gz /tmp/nxf.K3w4JnSYAS/tih_rna_sample_00636_B23MVV7LT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.K3w4JnSYAS/fi_workdir/tih_rna_sample_00636_B23MVV7LT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.K3w4JnSYAS/fi_workdir/tih_rna_sample_00636_B23MVV7LT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam tih_rna_sample_00636_B23MVV7LT4_1.star.sortedByCoord.out.bam * Running CMD: samtools index tih_rna_sample_00636_B23MVV7LT4_1.star.sortedByCoord.out.bam 11:57:45 : INFO : Done. -parsing GTF file: /tmp/nxf.K3w4JnSYAS/fi_workdir/tih_rna_sample_00636_B23MVV7LT4_1.gtf -parsing /tmp/nxf.K3w4JnSYAS/fi_workdir/tih_rna_sample_00636_B23MVV7LT4_1.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] [90000] [100000] [110000] [120000] [130000] [140000] [150000] [160000] [170000] [180000] [190000] [200000] [210000] [220000] [230000] [240000] [250000] [260000] [270000] [280000] [290000] [300000] [310000] [320000] [330000] [340000] [350000] [360000] [370000] [380000] [390000] [400000] [410000] [420000] [430000] [440000] [450000] [460000] [470000] [480000] [490000] [500000] [510000] [520000] [530000] [540000] [550000] [560000] [570000] [580000] [590000] [600000] [610000] [620000] [630000] [640000] [650000] [660000] [670000] [680000] [690000] [700000] [710000] [720000] [730000] [740000] [750000] [760000] [770000] [780000] [790000] [800000] [810000] [820000] [830000] -done parsing /tmp/nxf.K3w4JnSYAS/fi_workdir/tih_rna_sample_00636_B23MVV7LT4_1.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { 'num_hits: 3 != num_counted_on_fusion_contigs 2 ' => 10, 'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 51, 'small anchor length' => 1, 'num_hits: 4 != num_counted_on_fusion_contigs 1 ' => 2, 'exons hit < 2' => 1, ' ** passed ** ' => 7245, 'excessive soft clipping' => 4374, 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 29, 'num_hits: 4 != num_counted_on_fusion_contigs 3 ' => 6, 'num_hits: 6 != num_counted_on_fusion_contigs 4 ' => 248, 'num_hits: 6 != num_counted_on_fusion_contigs 3 ' => 9, 'per_id < 96' => 2096, 'low complexity anchor region' => 3, 'num genes matched < 2' => 12, 'num_hits: 4 != num_counted_on_fusion_contigs 2 ' => 30, 'seq-similar region overlap' => 114 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.K3w4JnSYAS/fi_workdir/tih_rna_sample_00636_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] -fusion SPANNING read extraction for scaff: AC079781.4--ASNS [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] -fusion SPANNING read extraction for scaff: AC092807.3--DDAH1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] -fusion SPANNING read extraction for scaff: ARHGEF35-AS1--ARHGEF35 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] [103000] [104000] [105000] [106000] [107000] [108000] [109000] [110000] [111000] [112000] [113000] [114000] [115000] [116000] [117000] [118000] [119000] [120000] [121000] [122000] [123000] [124000] [125000] [126000] [127000] [128000] [129000] [130000] [131000] [132000] [133000] [134000] [135000] [136000] [137000] [138000] [139000] [140000] [141000] [142000] [143000] [144000] [145000] [146000] [147000] [148000] [149000] [150000] [151000] [152000] [153000] [154000] [155000] [156000] [157000] [158000] [159000] [160000] [161000] [162000] [163000] [164000] [165000] [166000] [167000] [168000] [169000] -fusion SPANNING read extraction for scaff: FOXP1--ITPR1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] -fusion SPANNING read extraction for scaff: PCDHA3--PCDHAC1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] -fusion SPANNING read extraction for scaff: PCDHA3--PCDHAC2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] -fusion SPANNING read extraction for scaff: PCDHA3--PCDHA13 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] -fusion SPANNING read extraction for scaff: PCDHA5--PCDHAC1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] -fusion SPANNING read extraction for scaff: PCDHA5--PCDHAC2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] -fusion SPANNING read extraction for scaff: PCDHA5--PCDHA13 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] -fusion SPANNING read extraction for scaff: PCDHGA4--PCDHGA8 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] -fusion SPANNING read extraction for scaff: PCDHGA4--PCDHGB6 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] -fusion SPANNING read extraction for scaff: PCDHGA4--PCDHGC5 [1000] [2000] [3000] [4000] [5000] [6000] [7000] -fusion SPANNING read extraction for scaff: PCDHGA4--PCDHGC4 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] -fusion SPANNING read extraction for scaff: PCDHGA4--PCDHGC3 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] -fusion SPANNING read extraction for scaff: PCDHGA4--PCDHGA12 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] -fusion SPANNING read extraction for scaff: PCDHGA4--PCDHGA11 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] -fusion SPANNING read extraction for scaff: PCDHGA4--PCDHGA10 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] -fusion SPANNING read extraction for scaff: PCDHGA4--PCDHGB7 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] -fusion SPANNING read extraction for scaff: PCDHGA4--PCDHGA9 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] -fusion SPANNING read extraction for scaff: STX16--NPEPL1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] -fusion SPANNING read extraction for scaff: TLK2--AC240565.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] -fusion SPANNING read extraction for scaff: TLK2--FAM157A [1000] [2000] [3000] [4000] [5000] [6000] -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] -fusion SPANNING read extraction for scaff: TPRA1--FOXP1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 -filtered reads reasons: $VAR1 = { 'lacks exon overlap' => 10808, 'seq similar region alignment' => 20349 }; EM: Starting log likelihood: -4089.845449 EM: Round [1] log likelihood: -4087.765484 EM: Round [2] log likelihood: -4086.875296 EM: Round [3] log likelihood: -4086.177311 EM: Round [4] log likelihood: -4085.603521 EM: Round [5] log likelihood: -4085.125987 EM: Round [6] log likelihood: -4084.726649 EM: Round [7] log likelihood: -4084.391684 EM: Round [8] log likelihood: -4084.110013 EM: Round [9] log likelihood: -4083.872629 EM: Round [10] log likelihood: -4083.672159 EM: Round [11] log likelihood: -4083.502542 EM: Round [12] log likelihood: -4083.358774 EM: Round [13] log likelihood: -4083.236710 EM: Round [14] log likelihood: -4083.132909 EM: Round [15] log likelihood: -4083.044504 EM: Round [16] log likelihood: -4082.969102 EM: Round [17] log likelihood: -4082.904701 EM: Round [18] log likelihood: -4082.849621 EM: Round [19] log likelihood: -4082.802453 EM: Round [20] log likelihood: -4082.762009 EM: Round [21] log likelihood: -4082.727290 EM: Round [22] log likelihood: -4082.697450 EM: Round [23] log likelihood: -4082.671775 EM: Round [24] log likelihood: -4082.649660 EM: Round [25] log likelihood: -4082.630591 EM: Round [26] log likelihood: -4082.614132 EM: Round [27] log likelihood: -4082.599912 EM: Round [28] log likelihood: -4082.587615 EM: Round [29] log likelihood: -4082.576971 EM: Round [30] log likelihood: -4082.567750 EM: Round [31] log likelihood: -4082.559756 EM: Round [32] log likelihood: -4082.552819 EM: Round [33] log likelihood: -4082.546796 EM: Round [34] log likelihood: -4082.541561 EM: Round [35] log likelihood: -4082.537009 EM: Round [36] log likelihood: -4082.533048 EM: Round [37] log likelihood: -4082.529599 EM: Round [38] log likelihood: -4082.526594 EM: Round [39] log likelihood: -4082.523974 EM: Round [40] log likelihood: -4082.521689 EM: Round [41] log likelihood: -4082.519694 EM: Round [42] log likelihood: -4082.517952 EM: Round [43] log likelihood: -4082.516431 EM: Round [44] log likelihood: -4082.515100 EM: Round [45] log likelihood: -4082.513937 EM: Round [46] log likelihood: -4082.512920 EM: Round [47] log likelihood: -4082.512029 EM: Round [48] log likelihood: -4082.511249 EM: Round [49] log likelihood: -4082.510565 EM: Round [50] log likelihood: -4082.509967 EM: Round [51] log likelihood: -4082.509442 EM: Round [52] log likelihood: -4082.508981 EM: Round [53] log likelihood: -4082.508577 EM: Round [54] log likelihood: -4082.508222 EM: Round [55] log likelihood: -4082.507910 EM: Round [56] log likelihood: -4082.507637 EM: Round [57] log likelihood: -4082.507396 EM: Round [58] log likelihood: -4082.507184 EM: Round [59] log likelihood: -4082.506998 EM: Round [60] log likelihood: -4082.506834 EM: Round [61] log likelihood: -4082.506690 EM: Round [62] log likelihood: -4082.506562 EM: Round [63] log likelihood: -4082.506450 EM: Round [64] log likelihood: -4082.506351 EM: Stopping iterations at round 64 due to insufficient improvement in likelihood. [bam_sort_core] merging from 0 files and 8 in-memory blocks... -extracting read coordinates from /tmp/nxf.K3w4JnSYAS/tih_rna_sample_00636_B23MVV7LT4_1.consolidated.bam into /tmp/nxf.K3w4JnSYAS/tih_rna_sample_00636_B23MVV7LT4_1.consolidated.bam.read_coords CMD: touch /tmp/nxf.K3w4JnSYAS/tih_rna_sample_00636_B23MVV7LT4_1.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.K3w4JnSYAS/tih_rna_sample_00636_B23MVV7LT4_1.consolidated.bam.read_coords > /tmp/nxf.K3w4JnSYAS/tih_rna_sample_00636_B23MVV7LT4_1.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.K3w4JnSYAS/tih_rna_sample_00636_B23MVV7LT4_1.consolidated.bam.frag_coords > /tmp/nxf.K3w4JnSYAS/tih_rna_sample_00636_B23MVV7LT4_1.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.K3w4JnSYAS/fi_workdir/tih_rna_sample_00636_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.K3w4JnSYAS/fi_workdir/tih_rna_sample_00636_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.K3w4JnSYAS/fi_workdir/tih_rna_sample_00636_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.K3w4JnSYAS/tih_rna_sample_00636_B23MVV7LT4_1.post_blast_and_promiscuity_filter -done, see /tmp/nxf.K3w4JnSYAS/tih_rna_sample_00636_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -3481.463000 EM: Round [1] log likelihood: -3481.057729 EM: Round [2] log likelihood: -3481.057420 EM: Round [3] log likelihood: -3481.057419 EM: Stopping iterations at round 3 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.K3w4JnSYAS/tih_rna_sample_00636_B23MVV7LT4_1_1.fastp.fastq.gz: 278298367 Warning - not locating file: /tmp/nxf.K3w4JnSYAS/tih_rna_sample_00636_B23MVV7LT4_1.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000284627.1] WARNING, no entry stored in dbm for [ENSG00000284627.1] WARNING, no entry stored in dbm for [ENSG00000284627.1] WARNING, no entry stored in dbm for [ENSG00000280136.2] WARNING, no entry stored in dbm for [ENSG00000226049.3] WARNING, no entry stored in dbm for [ENSG00000260404.3] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000244198.7] WARNING, no entry stored in dbm for [ENSG00000244198.7] WARNING, no entry stored in dbm for [ENSG00000244198.7] ls: cannot access 'IGV_inputs': No such file or directory