08:04:08 : INFO : -reading targets list: ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
-mapping reads to genes
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[5010000], rate=13663636.36/min 08:04:37 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file tih_rna_sample_00685_B23MVV7LT4_1.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > tih_rna_sample_00685_B23MVV7LT4_1.cancer.introns.prelim
Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18.
-tih_rna_sample_00685_B23MVV7LT4_1.introns: identified 4378 cancer introns
08:04:38 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file tih_rna_sample_00685_B23MVV7LT4_1.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > tih_rna_sample_00685_B23MVV7LT4_1.cancer.introns.prelim
08:04:38 : INFO : Running: touch /tmp/nxf.fpOHsJ3KQk/tih_rna_sample_00685_B23MVV7LT4_1.chckpts/prelim_introns.ok
08:04:38 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates tih_rna_sample_00685_B23MVV7LT4_1.cancer.introns.prelim --min_total_reads 5 > tih_rna_sample_00685_B23MVV7LT4_1.cancer.introns
08:04:38 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates tih_rna_sample_00685_B23MVV7LT4_1.cancer.introns.prelim --min_total_reads 5 > tih_rna_sample_00685_B23MVV7LT4_1.cancer.introns
08:04:39 : INFO : Running: touch /tmp/nxf.fpOHsJ3KQk/tih_rna_sample_00685_B23MVV7LT4_1.chckpts/introns_filtered.ok
08:04:39 : INFO : -found 1128 cancer introns
08:04:39 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns tih_rna_sample_00685_B23MVV7LT4_1.introns --cancer_introns tih_rna_sample_00685_B23MVV7LT4_1.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed tih_rna_sample_00685_B23MVV7LT4_1.introns.for_IGV.bed
08:04:39 : INFO : Creating the BED File.
08:04:43 : INFO : Saving Bed File as tih_rna_sample_00685_B23MVV7LT4_1.introns.for_IGV.bed
08:04:43 : INFO : Execution Time = 0.08 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns tih_rna_sample_00685_B23MVV7LT4_1.introns --cancer_introns tih_rna_sample_00685_B23MVV7LT4_1.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed tih_rna_sample_00685_B23MVV7LT4_1.introns.for_IGV.bed
08:04:43 : INFO : Running: touch /tmp/nxf.fpOHsJ3KQk/tih_rna_sample_00685_B23MVV7LT4_1.chckpts/intron_igv_bed.ok
08:04:43 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed tih_rna_sample_00685_B23MVV7LT4_1.introns.for_IGV.bed --bam tih_rna_sample_00685_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam --output_prefix tih_rna_sample_00685_B23MVV7LT4_1
[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00685_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
08:13:05 : INFO : Execution Time = 8.36 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed tih_rna_sample_00685_B23MVV7LT4_1.introns.for_IGV.bed --bam tih_rna_sample_00685_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam --output_prefix tih_rna_sample_00685_B23MVV7LT4_1
08:13:05 : INFO : Running: touch /tmp/nxf.fpOHsJ3KQk/tih_rna_sample_00685_B23MVV7LT4_1.chckpts/reads_alignments_extracted.ok
08:13:05 : INFO : Running: samtools sort -o tih_rna_sample_00685_B23MVV7LT4_1.cancer_intron_reads.sorted.bam tih_rna_sample_00685_B23MVV7LT4_1.cancer_intron_reads.bam
08:13:05 : INFO : Execution Time = 0.00 minutes. CMD: samtools sort -o tih_rna_sample_00685_B23MVV7LT4_1.cancer_intron_reads.sorted.bam tih_rna_sample_00685_B23MVV7LT4_1.cancer_intron_reads.bam
08:13:05 : INFO : Running: touch /tmp/nxf.fpOHsJ3KQk/tih_rna_sample_00685_B23MVV7LT4_1.chckpts/sort_cancer_intron_reads.ok
08:13:05 : INFO : Running: samtools sort -o tih_rna_sample_00685_B23MVV7LT4_1.gene_reads.sorted.bam tih_rna_sample_00685_B23MVV7LT4_1.gene_reads.bam
[bam_sort_core] merging from 34 files and 1 in-memory blocks...
08:21:42 : INFO : Execution Time = 8.62 minutes. CMD: samtools sort -o tih_rna_sample_00685_B23MVV7LT4_1.gene_reads.sorted.bam tih_rna_sample_00685_B23MVV7LT4_1.gene_reads.bam
08:21:42 : INFO : Running: touch /tmp/nxf.fpOHsJ3KQk/tih_rna_sample_00685_B23MVV7LT4_1.chckpts/sort_gene_reads.ok
08:21:42 : INFO : Running: /usr/local/src/CTAT-SPLICING/bamsifter/bamsifter -c 50 -i 50 -o tih_rna_sample_00685_B23MVV7LT4_1.gene_reads.sorted.sifted.bam.tmp --keep_secondary tih_rna_sample_00685_B23MVV7LT4_1.gene_reads.sorted.bam
08:23:57 : INFO : Execution Time = 2.24 minutes. CMD: /usr/local/src/CTAT-SPLICING/bamsifter/bamsifter -c 50 -i 50 -o tih_rna_sample_00685_B23MVV7LT4_1.gene_reads.sorted.sifted.bam.tmp --keep_secondary tih_rna_sample_00685_B23MVV7LT4_1.gene_reads.sorted.bam
08:23:57 : INFO : Running: touch /tmp/nxf.fpOHsJ3KQk/tih_rna_sample_00685_B23MVV7LT4_1.chckpts/tih_rna_sample_00685_B23MVV7LT4_1.gene_reads.sorted.sifted.bam.tmp.ok
08:23:57 : INFO : Running: samtools sort -o tih_rna_sample_00685_B23MVV7LT4_1.gene_reads.sorted.sifted.bam tih_rna_sample_00685_B23MVV7LT4_1.gene_reads.sorted.sifted.bam.tmp
[bam_sort_core] merging from 1 files and 1 in-memory blocks...
08:24:20 : INFO : Execution Time = 0.39 minutes. CMD: samtools sort -o tih_rna_sample_00685_B23MVV7LT4_1.gene_reads.sorted.sifted.bam tih_rna_sample_00685_B23MVV7LT4_1.gene_reads.sorted.sifted.bam.tmp
08:24:20 : INFO : Running: touch /tmp/nxf.fpOHsJ3KQk/tih_rna_sample_00685_B23MVV7LT4_1.chckpts/tih_rna_sample_00685_B23MVV7LT4_1.gene_reads.sorted.sifted.bam.tmpsorted.ok
08:24:21 : INFO : Running: samtools index tih_rna_sample_00685_B23MVV7LT4_1.gene_reads.sorted.sifted.bam
08:24:24 : INFO : Execution Time = 0.05 minutes. CMD: samtools index tih_rna_sample_00685_B23MVV7LT4_1.gene_reads.sorted.sifted.bam
08:24:24 : INFO : Running: touch /tmp/nxf.fpOHsJ3KQk/tih_rna_sample_00685_B23MVV7LT4_1.chckpts/tih_rna_sample_00685_B23MVV7LT4_1.gene_reads.sorted.sifted.bam.indexed.ok
08:24:24 : INFO : Running: samtools index tih_rna_sample_00685_B23MVV7LT4_1.cancer_intron_reads.sorted.bam
08:24:24 : INFO : Execution Time = 0.00 minutes. CMD: samtools index tih_rna_sample_00685_B23MVV7LT4_1.cancer_intron_reads.sorted.bam
08:24:24 : INFO : Running: touch /tmp/nxf.fpOHsJ3KQk/tih_rna_sample_00685_B23MVV7LT4_1.chckpts/tih_rna_sample_00685_B23MVV7LT4_1.cancer_intron_reads.sorted.bam.indexed.ok
08:24:24 : INFO : Running: create_report tih_rna_sample_00685_B23MVV7LT4_1.introns.for_IGV.bed ctat_genome_lib_build_dir/ref_genome.fa --type junction --output tih_rna_sample_00685_B23MVV7LT4_1.ctat-splicing.igv.html --track-config tih_rna_sample_00685_B23MVV7LT4_1.igv.tracks --info-columns gene variant_name uniquely_mapped multi_mapped TCGA GTEx --title 'CTAT_Splicing: tih_rna_sample_00685_B23MVV7LT4_1'
08:29:03 : INFO : Execution Time = 4.66 minutes. CMD: create_report tih_rna_sample_00685_B23MVV7LT4_1.introns.for_IGV.bed ctat_genome_lib_build_dir/ref_genome.fa --type junction --output tih_rna_sample_00685_B23MVV7LT4_1.ctat-splicing.igv.html --track-config tih_rna_sample_00685_B23MVV7LT4_1.igv.tracks --info-columns gene variant_name uniquely_mapped multi_mapped TCGA GTEx --title 'CTAT_Splicing: tih_rna_sample_00685_B23MVV7LT4_1'
08:29:03 : INFO : Running: touch /tmp/nxf.fpOHsJ3KQk/tih_rna_sample_00685_B23MVV7LT4_1.chckpts/igv_create_html.ok
08:29:04 : INFO : done.