[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00584_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (1294055) splicing reads. 132398 splicing junctions. 123167 known splicing junctions. 9231 novel splicing junctions.
sampling 10% (2588111) splicing reads. 150133 splicing junctions. 136227 known splicing junctions. 13906 novel splicing junctions.
sampling 15% (3882167) splicing reads. 158306 splicing junctions. 141584 known splicing junctions. 16722 novel splicing junctions.
sampling 20% (5176223) splicing reads. 163418 splicing junctions. 144704 known splicing junctions. 18714 novel splicing junctions.
sampling 25% (6470279) splicing reads. 166759 splicing junctions. 146628 known splicing junctions. 20131 novel splicing junctions.
sampling 30% (7764334) splicing reads. 169048 splicing junctions. 147908 known splicing junctions. 21140 novel splicing junctions.
sampling 35% (9058390) splicing reads. 170773 splicing junctions. 148825 known splicing junctions. 21948 novel splicing junctions.
sampling 40% (10352446) splicing reads. 172200 splicing junctions. 149553 known splicing junctions. 22647 novel splicing junctions.
sampling 45% (11646502) splicing reads. 173259 splicing junctions. 150083 known splicing junctions. 23176 novel splicing junctions.
sampling 50% (12940558) splicing reads. 174126 splicing junctions. 150506 known splicing junctions. 23620 novel splicing junctions.
sampling 55% (14234613) splicing reads. 174860 splicing junctions. 150875 known splicing junctions. 23985 novel splicing junctions.
sampling 60% (15528669) splicing reads. 175466 splicing junctions. 151165 known splicing junctions. 24301 novel splicing junctions.
sampling 65% (16822725) splicing reads. 175972 splicing junctions. 151406 known splicing junctions. 24566 novel splicing junctions.
sampling 70% (18116781) splicing reads. 176374 splicing junctions. 151595 known splicing junctions. 24779 novel splicing junctions.
sampling 75% (19410837) splicing reads. 176701 splicing junctions. 151757 known splicing junctions. 24944 novel splicing junctions.
sampling 80% (20704892) splicing reads. 176937 splicing junctions. 151864 known splicing junctions. 25073 novel splicing junctions.
sampling 85% (21998948) splicing reads. 177146 splicing junctions. 151957 known splicing junctions. 25189 novel splicing junctions.
sampling 90% (23293004) splicing reads. 177326 splicing junctions. 152038 known splicing junctions. 25288 novel splicing junctions.
sampling 95% (24587060) splicing reads. 177416 splicing junctions. 152077 known splicing junctions. 25339 novel splicing junctions.
sampling 100% (25881116) splicing reads. 177467 splicing junctions. 152099 known splicing junctions. 25368 novel splicing junctions.