Done. -parsing GTF file: /tmp/nxf.vFikEdS7Kl/tih_rna_sample_00619_B23MVV7LT4_1.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.vFikEdS7Kl/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.vFikEdS7Kl/tih_rna_sample_00619_B23MVV7LT4_1_1.fastp.fastq.gz /tmp/nxf.vFikEdS7Kl/tih_rna_sample_00619_B23MVV7LT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.vFikEdS7Kl/fi_workdir/tih_rna_sample_00619_B23MVV7LT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.vFikEdS7Kl/fi_workdir/tih_rna_sample_00619_B23MVV7LT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam tih_rna_sample_00619_B23MVV7LT4_1.star.sortedByCoord.out.bam * Running CMD: samtools index tih_rna_sample_00619_B23MVV7LT4_1.star.sortedByCoord.out.bam 10:46:29 : INFO : Done. -parsing GTF file: /tmp/nxf.vFikEdS7Kl/fi_workdir/tih_rna_sample_00619_B23MVV7LT4_1.gtf -parsing /tmp/nxf.vFikEdS7Kl/fi_workdir/tih_rna_sample_00619_B23MVV7LT4_1.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] [90000] [100000] [110000] [120000] [130000] [140000] -done parsing /tmp/nxf.vFikEdS7Kl/fi_workdir/tih_rna_sample_00619_B23MVV7LT4_1.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { ' ** passed ** ' => 1117, 'seq-similar region overlap' => 295, 'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 1, 'per_id < 96' => 508, 'num genes matched < 2' => 13, 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 7, 'excessive soft clipping' => 1768, 'num_hits: 4 != num_counted_on_fusion_contigs 3 ' => 6, 'num_hits: 4 != num_counted_on_fusion_contigs 1 ' => 17 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.vFikEdS7Kl/fi_workdir/tih_rna_sample_00619_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] -fusion SPANNING read extraction for scaff: PCDHA10--PCDHAC2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] -fusion SPANNING read extraction for scaff: PCDHA10--PCDHAC1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] -fusion SPANNING read extraction for scaff: PCDHA10--PCDHA13 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] -fusion SPANNING read extraction for scaff: STX16--NPEPL1 [1000] [2000] [3000] [4000] -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 -filtered reads reasons: $VAR1 = { 'lacks exon overlap' => 296, 'seq similar region alignment' => 13143 }; EM: Starting log likelihood: -677.406070 EM: Round [1] log likelihood: -677.376483 EM: Round [2] log likelihood: -677.348231 EM: Round [3] log likelihood: -677.321237 EM: Round [4] log likelihood: -677.295430 EM: Round [5] log likelihood: -677.270744 EM: Round [6] log likelihood: -677.247115 EM: Round [7] log likelihood: -677.224488 EM: Round [8] log likelihood: -677.202810 EM: Round [9] log likelihood: -677.182031 EM: Round [10] log likelihood: -677.162105 EM: Round [11] log likelihood: -677.142989 EM: Round [12] log likelihood: -677.124644 EM: Round [13] log likelihood: -677.107030 EM: Round [14] log likelihood: -677.090114 EM: Round [15] log likelihood: -677.073861 EM: Round [16] log likelihood: -677.058242 EM: Round [17] log likelihood: -677.043226 EM: Round [18] log likelihood: -677.028786 EM: Round [19] log likelihood: -677.014896 EM: Round [20] log likelihood: -677.001531 EM: Round [21] log likelihood: -676.988669 EM: Round [22] log likelihood: -676.976287 EM: Round [23] log likelihood: -676.964364 EM: Round [24] log likelihood: -676.952882 EM: Round [25] log likelihood: -676.941821 EM: Round [26] log likelihood: -676.931163 EM: Round [27] log likelihood: -676.920893 EM: Round [28] log likelihood: -676.910993 EM: Round [29] log likelihood: -676.901450 EM: Round [30] log likelihood: -676.892247 EM: Round [31] log likelihood: -676.883373 EM: Round [32] log likelihood: -676.874813 EM: Round [33] log likelihood: -676.866556 EM: Round [34] log likelihood: -676.858589 EM: Round [35] log likelihood: -676.850901 EM: Round [36] log likelihood: -676.843481 EM: Round [37] log likelihood: -676.836320 EM: Round [38] log likelihood: -676.829406 EM: Round [39] log likelihood: -676.822732 EM: Round [40] log likelihood: -676.816287 EM: Round [41] log likelihood: -676.810063 EM: Round [42] log likelihood: -676.804052 EM: Round [43] log likelihood: -676.798246 EM: Round [44] log likelihood: -676.792638 EM: Round [45] log likelihood: -676.787220 EM: Round [46] log likelihood: -676.781985 EM: Round [47] log likelihood: -676.776927 EM: Round [48] log likelihood: -676.772039 EM: Round [49] log likelihood: -676.767315 EM: Round [50] log likelihood: -676.762749 EM: Round [51] log likelihood: -676.758336 EM: Round [52] log likelihood: -676.754070 EM: Round [53] log likelihood: -676.749946 EM: Round [54] log likelihood: -676.745959 EM: Round [55] log likelihood: -676.742105 EM: Round [56] log likelihood: -676.738377 EM: Round [57] log likelihood: -676.734773 EM: Round [58] log likelihood: -676.731288 EM: Round [59] log likelihood: -676.727917 EM: Round [60] log likelihood: -676.724657 EM: Round [61] log likelihood: -676.721504 EM: Round [62] log likelihood: -676.718455 EM: Round [63] log likelihood: -676.715505 EM: Round [64] log likelihood: -676.712651 EM: Round [65] log likelihood: -676.709890 EM: Round [66] log likelihood: -676.707220 EM: Round [67] log likelihood: -676.704636 EM: Round [68] log likelihood: -676.702136 EM: Round [69] log likelihood: -676.699717 EM: Round [70] log likelihood: -676.697377 EM: Round [71] log likelihood: -676.695112 EM: Round [72] log likelihood: -676.692920 EM: Round [73] log likelihood: -676.690800 EM: Round [74] log likelihood: -676.688747 EM: Round [75] log likelihood: -676.686761 EM: Round [76] log likelihood: -676.684839 EM: Round [77] log likelihood: -676.682979 EM: Round [78] log likelihood: -676.681178 EM: Round [79] log likelihood: -676.679435 EM: Round [80] log likelihood: -676.677748 EM: Round [81] log likelihood: -676.676115 EM: Round [82] log likelihood: -676.674535 EM: Round [83] log likelihood: -676.673005 EM: Round [84] log likelihood: -676.671524 EM: Round [85] log likelihood: -676.670090 EM: Round [86] log likelihood: -676.668702 EM: Round [87] log likelihood: -676.667359 EM: Round [88] log likelihood: -676.666058 EM: Round [89] log likelihood: -676.664799 EM: Round [90] log likelihood: -676.663580 EM: Round [91] log likelihood: -676.662399 EM: Round [92] log likelihood: -676.661256 EM: Round [93] log likelihood: -676.660150 EM: Round [94] log likelihood: -676.659079 EM: Round [95] log likelihood: -676.658041 EM: Round [96] log likelihood: -676.657037 EM: Round [97] log likelihood: -676.656064 EM: Round [98] log likelihood: -676.655123 EM: Round [99] log likelihood: -676.654211 EM: Round [100] log likelihood: -676.653328 EM: Round [101] log likelihood: -676.652473 EM: Round [102] log likelihood: -676.651645 EM: Round [103] log likelihood: -676.650843 EM: Round [104] log likelihood: -676.650067 EM: Round [105] log likelihood: -676.649315 EM: Round [106] log likelihood: -676.648587 EM: Round [107] log likelihood: -676.647882 EM: Round [108] log likelihood: -676.647199 EM: Round [109] log likelihood: -676.646538 EM: Round [110] log likelihood: -676.645898 EM: Round [111] log likelihood: -676.645278 EM: Round [112] log likelihood: -676.644677 EM: Round [113] log likelihood: -676.644095 EM: Round [114] log likelihood: -676.643532 EM: Round [115] log likelihood: -676.642987 EM: Round [116] log likelihood: -676.642458 EM: Round [117] log likelihood: -676.641947 EM: Round [118] log likelihood: -676.641451 EM: Round [119] log likelihood: -676.640971 EM: Round [120] log likelihood: -676.640506 EM: Round [121] log likelihood: -676.640056 EM: Round [122] log likelihood: -676.639620 EM: Round [123] log likelihood: -676.639197 EM: Round [124] log likelihood: -676.638788 EM: Round [125] log likelihood: -676.638392 EM: Round [126] log likelihood: -676.638008 EM: Round [127] log likelihood: -676.637637 EM: Round [128] log likelihood: -676.637277 EM: Round [129] log likelihood: -676.636928 EM: Round [130] log likelihood: -676.636590 EM: Round [131] log likelihood: -676.636263 EM: Round [132] log likelihood: -676.635946 EM: Round [133] log likelihood: -676.635639 EM: Round [134] log likelihood: -676.635342 EM: Round [135] log likelihood: -676.635054 EM: Round [136] log likelihood: -676.634775 EM: Round [137] log likelihood: -676.634505 EM: Round [138] log likelihood: -676.634243 EM: Round [139] log likelihood: -676.633990 EM: Round [140] log likelihood: -676.633744 EM: Round [141] log likelihood: -676.633507 EM: Round [142] log likelihood: -676.633276 EM: Round [143] log likelihood: -676.633053 EM: Round [144] log likelihood: -676.632837 EM: Round [145] log likelihood: -676.632628 EM: Round [146] log likelihood: -676.632425 EM: Round [147] log likelihood: -676.632228 EM: Round [148] log likelihood: -676.632038 EM: Round [149] log likelihood: -676.631854 EM: Round [150] log likelihood: -676.631675 EM: Round [151] log likelihood: -676.631502 EM: Round [152] log likelihood: -676.631335 EM: Round [153] log likelihood: -676.631173 EM: Round [154] log likelihood: -676.631015 EM: Round [155] log likelihood: -676.630863 EM: Round [156] log likelihood: -676.630716 EM: Round [157] log likelihood: -676.630573 EM: Round [158] log likelihood: -676.630434 EM: Round [159] log likelihood: -676.630300 EM: Round [160] log likelihood: -676.630170 EM: Round [161] log likelihood: -676.630045 EM: Round [162] log likelihood: -676.629923 EM: Round [163] log likelihood: -676.629805 EM: Round [164] log likelihood: -676.629690 EM: Round [165] log likelihood: -676.629580 EM: Round [166] log likelihood: -676.629472 EM: Round [167] log likelihood: -676.629368 EM: Round [168] log likelihood: -676.629268 EM: Round [169] log likelihood: -676.629170 EM: Stopping iterations at round 169 due to insufficient improvement in likelihood. [bam_sort_core] merging from 0 files and 8 in-memory blocks... -extracting read coordinates from /tmp/nxf.vFikEdS7Kl/tih_rna_sample_00619_B23MVV7LT4_1.consolidated.bam into /tmp/nxf.vFikEdS7Kl/tih_rna_sample_00619_B23MVV7LT4_1.consolidated.bam.read_coords CMD: touch /tmp/nxf.vFikEdS7Kl/tih_rna_sample_00619_B23MVV7LT4_1.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.vFikEdS7Kl/tih_rna_sample_00619_B23MVV7LT4_1.consolidated.bam.read_coords > /tmp/nxf.vFikEdS7Kl/tih_rna_sample_00619_B23MVV7LT4_1.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.vFikEdS7Kl/tih_rna_sample_00619_B23MVV7LT4_1.consolidated.bam.frag_coords > /tmp/nxf.vFikEdS7Kl/tih_rna_sample_00619_B23MVV7LT4_1.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.vFikEdS7Kl/fi_workdir/tih_rna_sample_00619_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.vFikEdS7Kl/fi_workdir/tih_rna_sample_00619_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.vFikEdS7Kl/fi_workdir/tih_rna_sample_00619_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.vFikEdS7Kl/tih_rna_sample_00619_B23MVV7LT4_1.post_blast_and_promiscuity_filter -done, see /tmp/nxf.vFikEdS7Kl/tih_rna_sample_00619_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -601.338526 EM: Round [1] log likelihood: -601.338526 EM: Stopping iterations at round 1 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.vFikEdS7Kl/tih_rna_sample_00619_B23MVV7LT4_1_1.fastp.fastq.gz: 241794911 Warning - not locating file: /tmp/nxf.vFikEdS7Kl/tih_rna_sample_00619_B23MVV7LT4_1.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000226049.3] WARNING, no entry stored in dbm for [ENSG00000260404.3] ls: cannot access 'IGV_inputs': No such file or directory