[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00668_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (21142943) splicing reads. 278709 splicing junctions. 186937 known splicing junctions. 91772 novel splicing junctions.
sampling 10% (42285886) splicing reads. 348880 splicing junctions. 200267 known splicing junctions. 148613 novel splicing junctions.
sampling 15% (63428829) splicing reads. 400327 splicing junctions. 207540 known splicing junctions. 192787 novel splicing junctions.
sampling 20% (84571772) splicing reads. 441603 splicing junctions. 212350 known splicing junctions. 229253 novel splicing junctions.
sampling 25% (105714716) splicing reads. 475979 splicing junctions. 216036 known splicing junctions. 259943 novel splicing junctions.
sampling 30% (126857659) splicing reads. 505290 splicing junctions. 218733 known splicing junctions. 286557 novel splicing junctions.
sampling 35% (148000602) splicing reads. 530512 splicing junctions. 220880 known splicing junctions. 309632 novel splicing junctions.
sampling 40% (169143545) splicing reads. 552911 splicing junctions. 222672 known splicing junctions. 330239 novel splicing junctions.
sampling 45% (190286488) splicing reads. 572562 splicing junctions. 224161 known splicing junctions. 348401 novel splicing junctions.
sampling 50% (211429432) splicing reads. 589771 splicing junctions. 225321 known splicing junctions. 364450 novel splicing junctions.
sampling 55% (232572375) splicing reads. 605172 splicing junctions. 226372 known splicing junctions. 378800 novel splicing junctions.
sampling 60% (253715318) splicing reads. 618764 splicing junctions. 227269 known splicing junctions. 391495 novel splicing junctions.
sampling 65% (274858261) splicing reads. 630565 splicing junctions. 228022 known splicing junctions. 402543 novel splicing junctions.
sampling 70% (296001204) splicing reads. 641055 splicing junctions. 228672 known splicing junctions. 412383 novel splicing junctions.
sampling 75% (317144148) splicing reads. 650334 splicing junctions. 229223 known splicing junctions. 421111 novel splicing junctions.
sampling 80% (338287091) splicing reads. 658349 splicing junctions. 229708 known splicing junctions. 428641 novel splicing junctions.
sampling 85% (359430034) splicing reads. 665184 splicing junctions. 230126 known splicing junctions. 435058 novel splicing junctions.
sampling 90% (380572977) splicing reads. 671098 splicing junctions. 230491 known splicing junctions. 440607 novel splicing junctions.
sampling 95% (401715920) splicing reads. 675978 splicing junctions. 230769 known splicing junctions. 445209 novel splicing junctions.
sampling 100% (422858864) splicing reads. 679846 splicing junctions. 231001 known splicing junctions. 448845 novel splicing junctions.