Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f8/e88fd7fc7045f143707b3a331bf8bc/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/73/d4dc4bd22a1948596a7cfde8e71603/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/73/d4dc4bd22a1948596a7cfde8e71603/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1c/0f71a0c154cf351fb20ae48f0bca50/tih_rna_sample_00582_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/67/06c2d4721a1880f0c93d310501f4f9/tih_rna_sample_00582_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00582_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (15594391) splicing reads. 281341 splicing junctions. 194115 known splicing junctions. 87226 novel splicing junctions.
sampling 10% (31188783) splicing reads. 343210 splicing junctions. 206487 known splicing junctions. 136723 novel splicing junctions.
sampling 15% (46783175) splicing reads. 385914 splicing junctions. 212893 known splicing junctions. 173021 novel splicing junctions.
sampling 20% (62377567) splicing reads. 418051 splicing junctions. 216878 known splicing junctions. 201173 novel splicing junctions.
sampling 25% (77971959) splicing reads. 443964 splicing junctions. 219836 known splicing junctions. 224128 novel splicing junctions.
sampling 30% (93566351) splicing reads. 464973 splicing junctions. 221952 known splicing junctions. 243021 novel splicing junctions.
sampling 35% (109160743) splicing reads. 482587 splicing junctions. 223737 known splicing junctions. 258850 novel splicing junctions.
sampling 40% (124755135) splicing reads. 497530 splicing junctions. 225132 known splicing junctions. 272398 novel splicing junctions.
sampling 45% (140349527) splicing reads. 510281 splicing junctions. 226261 known splicing junctions. 284020 novel splicing junctions.
sampling 50% (155943919) splicing reads. 521333 splicing junctions. 227205 known splicing junctions. 294128 novel splicing junctions.
sampling 55% (171538310) splicing reads. 530696 splicing junctions. 227973 known splicing junctions. 302723 novel splicing junctions.
sampling 60% (187132702) splicing reads. 538874 splicing junctions. 228660 known splicing junctions. 310214 novel splicing junctions.
sampling 65% (202727094) splicing reads. 545918 splicing junctions. 229227 known splicing junctions. 316691 novel splicing junctions.
sampling 70% (218321486) splicing reads. 551888 splicing junctions. 229679 known splicing junctions. 322209 novel splicing junctions.
sampling 75% (233915878) splicing reads. 557160 splicing junctions. 230082 known splicing junctions. 327078 novel splicing junctions.
sampling 80% (249510270) splicing reads. 561561 splicing junctions. 230374 known splicing junctions. 331187 novel splicing junctions.
sampling 85% (265104662) splicing reads. 565250 splicing junctions. 230628 known splicing junctions. 334622 novel splicing junctions.
sampling 90% (280699054) splicing reads. 568322 splicing junctions. 230864 known splicing junctions. 337458 novel splicing junctions.
sampling 95% (296293446) splicing reads. 570727 splicing junctions. 231056 known splicing junctions. 339671 novel splicing junctions.
sampling 100% (311887838) splicing reads. 572615 splicing junctions. 231217 known splicing junctions. 341398 novel splicing junctions.
null device 
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