+ mkdir FFPE_HD789_01_RNA_13_B23MVV7LT4_1 + all_qc -bam FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder FFPE_HD789_01_RNA_13_B23MVV7LT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai 90817it [00:00, 908092.92it/s] 225656it [00:00, 1148048.92it/s] 363869it [00:00, 1253719.76it/s] 508170it [00:00, 1328004.67it/s] 658928it [00:00, 1392087.33it/s] 798162it [00:00, 1366198.18it/s] 964142it [00:00, 1460775.68it/s] 1119233it [00:00, 1489157.90it/s] 1278189it [00:00, 1520294.94it/s] 1433222it [00:01, 1529489.25it/s] 1586270it [00:01, 1514547.26it/s] 1737822it [00:01, 1511178.08it/s] 1889006it [00:01, 1489635.61it/s] 2047480it [00:01, 1517869.99it/s] 2199381it [00:01, 1483762.05it/s] 2353425it [00:01, 1500281.17it/s] 2513208it [00:01, 1529088.20it/s] 2672683it [00:01, 1548550.76it/s] 2827689it [00:01, 1388158.88it/s] 2969657it [00:02, 1373740.71it/s] 3109176it [00:02, 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1629432.04it/s] 7698951it [00:05, 1609149.14it/s] 7860559it [00:05, 1611151.61it/s] 8021810it [00:05, 1606663.49it/s] 8182568it [00:05, 1602729.72it/s] 8349254it [00:05, 1621728.47it/s] 8516228it [00:05, 1635993.43it/s] 8685093it [00:05, 1651546.58it/s] 8853836it [00:05, 1662245.55it/s] 9021368it [00:06, 1666126.78it/s] 9191977it [00:06, 1678059.67it/s] 9359805it [00:06, 1672483.93it/s] 9527072it [00:06, 1667774.76it/s] 9695814it [00:06, 1673611.23it/s] 9863188it [00:06, 1673000.04it/s] 10031104it [00:06, 1674809.42it/s] 10198592it [00:06, 1660018.00it/s] 10364626it [00:06, 1638656.64it/s] 10534440it [00:06, 1656214.15it/s] 10704377it [00:07, 1669000.86it/s] 10872102it [00:07, 1671424.21it/s] 11039292it [00:07, 1671275.29it/s] 11208428it [00:07, 1677244.83it/s] 11379326it [00:07, 1686705.62it/s] 11548795it [00:07, 1689063.36it/s] 11717716it [00:07, 1688367.24it/s] 11886563it [00:07, 1658770.45it/s] 12052561it [00:07, 1652830.44it/s] 12221887it [00:07, 1664780.05it/s] 12388437it [00:08, 1548272.19it/s] 12553017it [00:08, 1575753.75it/s] 12719873it [00:08, 1602417.24it/s] 12881133it [00:08, 1594491.97it/s] 13045001it [00:08, 1607389.71it/s] 13212536it [00:08, 1627389.42it/s] 13378793it [00:08, 1637784.69it/s] 13542866it [00:08, 1623190.68it/s] 13705411it [00:08, 1606467.39it/s] 13866237it [00:09, 1499471.57it/s] 14030074it [00:09, 1538561.38it/s] 14193288it [00:09, 1565357.29it/s] 14357244it [00:09, 1586926.95it/s] 14524853it [00:09, 1613079.83it/s] 14687653it [00:09, 1617461.20it/s] 14849820it [00:09, 1615979.85it/s] 15018438it [00:09, 1636821.11it/s] 15185717it [00:09, 1647514.62it/s] 15301882it [00:09, 1541552.37it/s] [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md.bai + merge_qc -folders FFPE_HD789_01_RNA_13_B23MVV7LT4_1/FFPE_HD789_01_RNA_13_B23MVV7LT4_1.md -output FFPE_HD789_01_RNA_13_B23MVV7LT4_1/FFPE_HD789_01_RNA_13_B23MVV7LT4_1_full_basic_stats.tsv + head -n1 FFPE_HD789_01_RNA_13_B23MVV7LT4_1/FFPE_HD789_01_RNA_13_B23MVV7LT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed FFPE_HD789_01_RNA_13_B23MVV7LT4_1/FFPE_HD789_01_RNA_13_B23MVV7LT4_1_full_basic_stats.tsv