[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00599_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (12980786) splicing reads. 258606 splicing junctions. 183625 known splicing junctions. 74981 novel splicing junctions.
sampling 10% (25961573) splicing reads. 312715 splicing junctions. 195443 known splicing junctions. 117272 novel splicing junctions.
sampling 15% (38942360) splicing reads. 348463 splicing junctions. 201587 known splicing junctions. 146876 novel splicing junctions.
sampling 20% (51923147) splicing reads. 374603 splicing junctions. 205438 known splicing junctions. 169165 novel splicing junctions.
sampling 25% (64903934) splicing reads. 395123 splicing junctions. 208115 known splicing junctions. 187008 novel splicing junctions.
sampling 30% (77884720) splicing reads. 411530 splicing junctions. 210138 known splicing junctions. 201392 novel splicing junctions.
sampling 35% (90865507) splicing reads. 424853 splicing junctions. 211677 known splicing junctions. 213176 novel splicing junctions.
sampling 40% (103846294) splicing reads. 436180 splicing junctions. 212933 known splicing junctions. 223247 novel splicing junctions.
sampling 45% (116827081) splicing reads. 445598 splicing junctions. 213942 known splicing junctions. 231656 novel splicing junctions.
sampling 50% (129807868) splicing reads. 453505 splicing junctions. 214756 known splicing junctions. 238749 novel splicing junctions.
sampling 55% (142788654) splicing reads. 460237 splicing junctions. 215458 known splicing junctions. 244779 novel splicing junctions.
sampling 60% (155769441) splicing reads. 466042 splicing junctions. 216058 known splicing junctions. 249984 novel splicing junctions.
sampling 65% (168750228) splicing reads. 470769 splicing junctions. 216493 known splicing junctions. 254276 novel splicing junctions.
sampling 70% (181731015) splicing reads. 474860 splicing junctions. 216912 known splicing junctions. 257948 novel splicing junctions.
sampling 75% (194711802) splicing reads. 478225 splicing junctions. 217223 known splicing junctions. 261002 novel splicing junctions.
sampling 80% (207692588) splicing reads. 481065 splicing junctions. 217479 known splicing junctions. 263586 novel splicing junctions.
sampling 85% (220673375) splicing reads. 483296 splicing junctions. 217696 known splicing junctions. 265600 novel splicing junctions.
sampling 90% (233654162) splicing reads. 485058 splicing junctions. 217848 known splicing junctions. 267210 novel splicing junctions.
sampling 95% (246634949) splicing reads. 486349 splicing junctions. 217965 known splicing junctions. 268384 novel splicing junctions.
sampling 100% (259615736) splicing reads. 487184 splicing junctions. 218040 known splicing junctions. 269144 novel splicing junctions.