Done. -parsing GTF file: /tmp/nxf.gXp9MDBVBB/tih_rna_sample_00652_B23MVV7LT4_1.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.gXp9MDBVBB/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.gXp9MDBVBB/tih_rna_sample_00652_B23MVV7LT4_1_1.fastp.fastq.gz /tmp/nxf.gXp9MDBVBB/tih_rna_sample_00652_B23MVV7LT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.gXp9MDBVBB/fi_workdir/tih_rna_sample_00652_B23MVV7LT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.gXp9MDBVBB/fi_workdir/tih_rna_sample_00652_B23MVV7LT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam tih_rna_sample_00652_B23MVV7LT4_1.star.sortedByCoord.out.bam * Running CMD: samtools index tih_rna_sample_00652_B23MVV7LT4_1.star.sortedByCoord.out.bam 11:45:16 : INFO : Done. -parsing GTF file: /tmp/nxf.gXp9MDBVBB/fi_workdir/tih_rna_sample_00652_B23MVV7LT4_1.gtf -parsing /tmp/nxf.gXp9MDBVBB/fi_workdir/tih_rna_sample_00652_B23MVV7LT4_1.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] [90000] [100000] [110000] [120000] [130000] [140000] [150000] [160000] [170000] [180000] [190000] [200000] [210000] [220000] [230000] [240000] [250000] [260000] [270000] [280000] [290000] [300000] [310000] [320000] [330000] [340000] [350000] [360000] [370000] [380000] [390000] [400000] [410000] [420000] [430000] [440000] [450000] [460000] [470000] [480000] [490000] [500000] [510000] [520000] [530000] [540000] [550000] [560000] [570000] [580000] [590000] [600000] [610000] [620000] [630000] [640000] [650000] [660000] [670000] -done parsing /tmp/nxf.gXp9MDBVBB/fi_workdir/tih_rna_sample_00652_B23MVV7LT4_1.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { 'low complexity anchor region' => 7, 'num_hits: 3 != num_counted_on_fusion_contigs 2 ' => 30, 'exons hit < 2' => 1, 'seq-similar region overlap' => 38, 'num_hits: 4 != num_counted_on_fusion_contigs 3 ' => 6, ' ** passed ** ' => 3873, 'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 7, 'num_hits: 4 != num_counted_on_fusion_contigs 1 ' => 14, 'num_hits: 4 != num_counted_on_fusion_contigs 2 ' => 196, 'per_id < 96' => 2126, 'small anchor length' => 19, 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 34, 'num genes matched < 2' => 6, 'excessive soft clipping' => 5167 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.gXp9MDBVBB/fi_workdir/tih_rna_sample_00652_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] -fusion SPANNING read extraction for scaff: AC012174.1--TTLL5 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] -fusion SPANNING read extraction for scaff: AGAP14P--FRMPD2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] -fusion SPANNING read extraction for scaff: CUL3--UNC80 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] -fusion SPANNING read extraction for scaff: FAM13A--TMEM65 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] -fusion SPANNING read extraction for scaff: FAM13A--TRMT12 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] [103000] [104000] [105000] [106000] [107000] [108000] [109000] [110000] [111000] [112000] [113000] [114000] [115000] [116000] [117000] [118000] [119000] [120000] [121000] [122000] [123000] [124000] -fusion SPANNING read extraction for scaff: KIAA1217--RET [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHA13 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] -fusion SPANNING read extraction for scaff: PCDHA4--PCDHAC1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] -fusion SPANNING read extraction for scaff: PCDHA4--PCDHAC2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] -fusion SPANNING read extraction for scaff: PCDHA4--PCDHA13 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA10 [1000] [2000] [3000] [4000] [5000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGC5 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA11 [1000] [2000] [3000] [4000] [5000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGC4 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA12 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGB6 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA8 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGC3 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGB7 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] -fusion SPANNING read extraction for scaff: PCDHGB2--PCDHGA9 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] [103000] [104000] [105000] [106000] [107000] [108000] [109000] [110000] [111000] [112000] [113000] [114000] [115000] [116000] [117000] [118000] [119000] [120000] [121000] [122000] [123000] [124000] [125000] [126000] [127000] [128000] [129000] [130000] [131000] [132000] [133000] [134000] [135000] [136000] [137000] [138000] [139000] [140000] [141000] [142000] [143000] [144000] [145000] [146000] [147000] [148000] [149000] [150000] [151000] [152000] [153000] [154000] -fusion SPANNING read extraction for scaff: PDLIM5--KIAA1217 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] -fusion SPANNING read extraction for scaff: RRM2B--BMPR1B [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] -fusion SPANNING read extraction for scaff: SLC30A9--RASSF6 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] -fusion SPANNING read extraction for scaff: TLK2--AC240565.1 [1000] [2000] [3000] [4000] -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 -filtered reads reasons: $VAR1 = { 'lacks exon overlap' => 2729, 'seq similar region alignment' => 26570 }; EM: Starting log likelihood: -2427.915482 EM: Round [1] log likelihood: -2427.707599 EM: Round [2] log likelihood: -2427.541445 EM: Round [3] log likelihood: -2427.399892 EM: Round [4] log likelihood: -2427.277660 EM: Round [5] log likelihood: -2427.171030 EM: Round [6] log likelihood: -2427.077266 EM: Round [7] log likelihood: -2426.994306 EM: Round [8] log likelihood: -2426.920551 EM: Round [9] log likelihood: -2426.854731 EM: Round [10] log likelihood: -2426.795814 EM: Round [11] log likelihood: -2426.742944 EM: Round [12] log likelihood: -2426.695398 EM: Round [13] log likelihood: -2426.652560 EM: Round [14] log likelihood: -2426.613893 EM: Round [15] log likelihood: -2426.578931 EM: Round [16] log likelihood: -2426.547266 EM: Round [17] log likelihood: -2426.518535 EM: Round [18] log likelihood: -2426.492420 EM: Round [19] log likelihood: -2426.468639 EM: Round [20] log likelihood: -2426.446940 EM: Round [21] log likelihood: -2426.427103 EM: Round [22] log likelihood: -2426.408930 EM: Round [23] log likelihood: -2426.392246 EM: Round [24] log likelihood: -2426.376896 EM: Round [25] log likelihood: -2426.362744 EM: Round [26] log likelihood: -2426.349667 EM: Round [27] log likelihood: -2426.337557 EM: Round [28] log likelihood: -2426.326319 EM: Round [29] log likelihood: -2426.315868 EM: Round [30] log likelihood: -2426.306129 EM: Round [31] log likelihood: -2426.297035 EM: Round [32] log likelihood: -2426.288527 EM: Round [33] log likelihood: -2426.280552 EM: Round [34] log likelihood: -2426.273064 EM: Round [35] log likelihood: -2426.266021 EM: Round [36] log likelihood: -2426.259385 EM: Round [37] log likelihood: -2426.253125 EM: Round [38] log likelihood: -2426.247210 EM: Round [39] log likelihood: -2426.241614 EM: Round [40] log likelihood: -2426.236313 EM: Round [41] log likelihood: -2426.231286 EM: Round [42] log likelihood: -2426.226513 EM: Round [43] log likelihood: -2426.221978 EM: Round [44] log likelihood: -2426.217664 EM: Round [45] log likelihood: -2426.213557 EM: Round [46] log likelihood: -2426.209644 EM: Round [47] log likelihood: -2426.205914 EM: Round [48] log likelihood: -2426.202355 EM: Round [49] log likelihood: -2426.198958 EM: Round [50] log likelihood: -2426.195713 EM: Round [51] log likelihood: -2426.192612 EM: Round [52] log likelihood: -2426.189648 EM: Round [53] log likelihood: -2426.186813 EM: Round [54] log likelihood: -2426.184100 EM: Round [55] log likelihood: -2426.181503 EM: Round [56] log likelihood: -2426.179017 EM: Round [57] log likelihood: -2426.176636 EM: Round [58] log likelihood: -2426.174355 EM: Round [59] log likelihood: -2426.172170 EM: Round [60] log likelihood: -2426.170075 EM: Round [61] log likelihood: -2426.168066 EM: Round [62] log likelihood: -2426.166141 EM: Round [63] log likelihood: -2426.164294 EM: Round [64] log likelihood: -2426.162523 EM: Round [65] log likelihood: -2426.160824 EM: Round [66] log likelihood: -2426.159194 EM: Round [67] log likelihood: -2426.157629 EM: Round [68] log likelihood: -2426.156128 EM: Round [69] log likelihood: -2426.154687 EM: Round [70] log likelihood: -2426.153304 EM: Round [71] log likelihood: -2426.151977 EM: Round [72] log likelihood: -2426.150702 EM: Round [73] log likelihood: -2426.149478 EM: Round [74] log likelihood: -2426.148303 EM: Round [75] log likelihood: -2426.147175 EM: Round [76] log likelihood: -2426.146091 EM: Round [77] log likelihood: -2426.145051 EM: Round [78] log likelihood: -2426.144051 EM: Round [79] log likelihood: -2426.143091 EM: Round [80] log likelihood: -2426.142169 EM: Round [81] log likelihood: -2426.141283 EM: Round [82] log likelihood: -2426.140433 EM: Round [83] log likelihood: -2426.139615 EM: Round [84] log likelihood: -2426.138830 EM: Round [85] log likelihood: -2426.138075 EM: Round [86] log likelihood: -2426.137350 EM: Round [87] log likelihood: -2426.136654 EM: Round [88] log likelihood: -2426.135985 EM: Round [89] log likelihood: -2426.135342 EM: Round [90] log likelihood: -2426.134724 EM: Round [91] log likelihood: -2426.134130 EM: Round [92] log likelihood: -2426.133559 EM: Round [93] log likelihood: -2426.133011 EM: Round [94] log likelihood: -2426.132484 EM: Round [95] log likelihood: -2426.131978 EM: Round [96] log likelihood: -2426.131491 EM: Round [97] log likelihood: -2426.131023 EM: Round [98] log likelihood: -2426.130574 EM: Round [99] log likelihood: -2426.130142 EM: Round [100] log likelihood: -2426.129726 EM: Round [101] log likelihood: -2426.129327 EM: Round [102] log likelihood: -2426.128944 EM: Round [103] log likelihood: -2426.128575 EM: Round [104] log likelihood: -2426.128220 EM: Round [105] log likelihood: -2426.127880 EM: Round [106] log likelihood: -2426.127552 EM: Round [107] log likelihood: -2426.127238 EM: Round [108] log likelihood: -2426.126935 EM: Round [109] log likelihood: -2426.126644 EM: Round [110] log likelihood: -2426.126365 EM: Round [111] log likelihood: -2426.126096 EM: Round [112] log likelihood: -2426.125838 EM: Round [113] log likelihood: -2426.125589 EM: Round [114] log likelihood: -2426.125350 EM: Round [115] log likelihood: -2426.125121 EM: Round [116] log likelihood: -2426.124900 EM: Round [117] log likelihood: -2426.124688 EM: Round [118] log likelihood: -2426.124484 EM: Round [119] log likelihood: -2426.124288 EM: Round [120] log likelihood: -2426.124100 EM: Round [121] log likelihood: -2426.123919 EM: Round [122] log likelihood: -2426.123745 EM: Round [123] log likelihood: -2426.123577 EM: Round [124] log likelihood: -2426.123416 EM: Round [125] log likelihood: -2426.123262 EM: Round [126] log likelihood: -2426.123113 EM: Round [127] log likelihood: -2426.122970 EM: Round [128] log likelihood: -2426.122832 EM: Round [129] log likelihood: -2426.122700 EM: Round [130] log likelihood: -2426.122573 EM: Round [131] log likelihood: -2426.122451 EM: Round [132] log likelihood: -2426.122333 EM: Round [133] log likelihood: -2426.122220 EM: Round [134] log likelihood: -2426.122112 EM: Round [135] log likelihood: -2426.122007 EM: Round [136] log likelihood: -2426.121907 EM: Round [137] log likelihood: -2426.121810 EM: Stopping iterations at round 137 due to insufficient improvement in likelihood. [bam_sort_core] merging from 0 files and 8 in-memory blocks... -extracting read coordinates from /tmp/nxf.gXp9MDBVBB/tih_rna_sample_00652_B23MVV7LT4_1.consolidated.bam into /tmp/nxf.gXp9MDBVBB/tih_rna_sample_00652_B23MVV7LT4_1.consolidated.bam.read_coords CMD: touch /tmp/nxf.gXp9MDBVBB/tih_rna_sample_00652_B23MVV7LT4_1.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.gXp9MDBVBB/tih_rna_sample_00652_B23MVV7LT4_1.consolidated.bam.read_coords > /tmp/nxf.gXp9MDBVBB/tih_rna_sample_00652_B23MVV7LT4_1.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.gXp9MDBVBB/tih_rna_sample_00652_B23MVV7LT4_1.consolidated.bam.frag_coords > /tmp/nxf.gXp9MDBVBB/tih_rna_sample_00652_B23MVV7LT4_1.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.gXp9MDBVBB/fi_workdir/tih_rna_sample_00652_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.gXp9MDBVBB/fi_workdir/tih_rna_sample_00652_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.gXp9MDBVBB/fi_workdir/tih_rna_sample_00652_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.gXp9MDBVBB/tih_rna_sample_00652_B23MVV7LT4_1.post_blast_and_promiscuity_filter -done, see /tmp/nxf.gXp9MDBVBB/tih_rna_sample_00652_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -2026.099102 EM: Round [1] log likelihood: -2026.089288 EM: Round [2] log likelihood: -2026.089208 EM: Stopping iterations at round 2 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.gXp9MDBVBB/tih_rna_sample_00652_B23MVV7LT4_1_1.fastp.fastq.gz: 295852739 Warning - not locating file: /tmp/nxf.gXp9MDBVBB/tih_rna_sample_00652_B23MVV7LT4_1.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000279058.2] WARNING, no entry stored in dbm for [ENSG00000279058.2] WARNING, no entry stored in dbm for [ENSG00000280136.2] WARNING, no entry stored in dbm for [ENSG00000226049.3] WARNING, no entry stored in dbm for [ENSG00000260404.3] WARNING, no entry stored in dbm for [ENSG00000260115.1] ls: cannot access 'IGV_inputs': No such file or directory