Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/87/69e4a35f885cab5a8a44b61a33e990/tih_rna_sample_00669_B23MVV7LT4_1.fusionreport.tsv Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5a/0e5bee06e74f95eb07b7b8b4786f7b/tih_rna_sample_00669_B23MVV7LT4_1_2.fastp.fastq.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a9/1a8b2e55c1466f7131982dacc07cc9/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5a/0e5bee06e74f95eb07b7b8b4786f7b/tih_rna_sample_00669_B23MVV7LT4_1_1.fastp.fastq.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a9/1a8b2e55c1466f7131982dacc07cc9/.command.run ==> STAGING COMPLETE (6 inputs) Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_pair_to_mini_genome_join.pl --fusions tih_rna_sample_00669_B23MVV7LT4_1.fusionreport.tsv --gtf /tmp/nxf.ZB9XBvOyXZ/ctat_genome_lib_build_dir/ref_annot.gtf --genome_fa /tmp/nxf.ZB9XBvOyXZ/ctat_genome_lib_build_dir/ref_genome.fa --out_prefix /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1 --shrink_introns --max_intron_length 1000 Done. Execution Time = 0.12 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_pair_to_mini_genome_join.pl --fusions tih_rna_sample_00669_B23MVV7LT4_1.fusionreport.tsv --gtf /tmp/nxf.ZB9XBvOyXZ/ctat_genome_lib_build_dir/ref_annot.gtf --genome_fa /tmp/nxf.ZB9XBvOyXZ/ctat_genome_lib_build_dir/ref_genome.fa --out_prefix /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1 --shrink_introns --max_intron_length 1000 Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/fusion_contigs.ok Running: cp /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.fa /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fa Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.fa /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fa Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/cp_contigs_file_workdir Running: cp /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.gtf /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.gtf Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.gtf /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.gtf Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/cp_gtf_file_workdir.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fasta_and_gtf_to_cytoband.pl /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.fa /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.gtf > /tmp/nxf.ZB9XBvOyXZ/cytoBand.txt Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fasta_and_gtf_to_cytoband.pl /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.fa /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.gtf > /tmp/nxf.ZB9XBvOyXZ/cytoBand.txt Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/cytoband.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/gtf_gene_to_bed.pl /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.gtf > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.bed -parsing GTF file: /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.gtf Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/gtf_gene_to_bed.pl /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.gtf > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.bed Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/merged_contig_gtf_to_bed.ok Running: sort -k1,1 -k2,2n /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.bed > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.bed.sorted.bed Execution Time = 0.00 minutes. CMD: sort -k1,1 -k2,2n /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.bed > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.bed.sorted.bed Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/tih_rna_sample_00669_B23MVV7LT4_1.bed.bedsort.ok Running: bgzip -f /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.bed.sorted.bed Execution Time = 0.00 minutes. CMD: bgzip -f /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.bed.sorted.bed Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/tih_rna_sample_00669_B23MVV7LT4_1.bed.bgzip.ok Running: tabix -p bed /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.bed.sorted.bed.gz Execution Time = 0.00 minutes. CMD: tabix -p bed /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.bed.sorted.bed.gz Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/tih_rna_sample_00669_B23MVV7LT4_1.bed.tabix.ok Running: samtools faidx /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.fa Execution Time = 0.00 minutes. CMD: samtools faidx /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.fa Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/merged_contig_fai.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/run_FI_STAR.pl --genome /tmp/nxf.ZB9XBvOyXZ/ctat_genome_lib_build_dir/ref_genome.fa --patch /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fa --max_mate_dist 100000 -G /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.gtf --CPU 8 --out_prefix tih_rna_sample_00669_B23MVV7LT4_1.star --out_dir /tmp/nxf.ZB9XBvOyXZ/fi_workdir --reads "/tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1_1.fastp.fastq.gz /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1_2.fastp.fastq.gz" * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.ZB9XBvOyXZ/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1_1.fastp.fastq.gz /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' Jun 08 10:01:16 ..... started STAR run Jun 08 10:01:16 ..... loading genome Jun 08 10:02:55 ... generating Suffix Array index Jun 08 10:06:37 ... completed Suffix Array index Jun 08 10:06:37 ..... processing annotations GTF Jun 08 10:06:37 ..... inserting junctions into the genome indices Jun 08 10:07:38 ..... started mapping Jun 08 11:03:38 ..... finished mapping Jun 08 11:03:39 ..... started sorting BAM Jun 08 11:03:40 ..... finished successfully * Running CMD: mv Aligned.sortedByCoord.out.bam tih_rna_sample_00669_B23MVV7LT4_1.star.sortedByCoord.out.bam * Running CMD: samtools index tih_rna_sample_00669_B23MVV7LT4_1.star.sortedByCoord.out.bam Execution Time = 62.41 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/run_FI_STAR.pl --genome /tmp/nxf.ZB9XBvOyXZ/ctat_genome_lib_build_dir/ref_genome.fa --patch /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fa --max_mate_dist 100000 -G /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.gtf --CPU 8 --out_prefix tih_rna_sample_00669_B23MVV7LT4_1.star --out_dir /tmp/nxf.ZB9XBvOyXZ/fi_workdir --reads "/tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1_1.fastp.fastq.gz /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1_2.fastp.fastq.gz" Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/run_STAR.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/bam_mark_duplicates.py -i /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.sortedByCoord.out.bam -o /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam --remove_dups 11:03:46 : INFO : Done. Execution Time = 0.09 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/bam_mark_duplicates.py -i /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.sortedByCoord.out.bam -o /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam --remove_dups Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/mark_dup_reads.ok Running: samtools index /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam Execution Time = 0.01 minutes. CMD: samtools index /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/mark_dups_reads.index.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/get_fusion_JUNCTION_reads_from_fusion_contig_bam.pl --gtf_file /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.gtf --MIN_ALIGN_PER_ID 96 --bam /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam --genome_lib_dir ctat_genome_lib_build_dir > /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam -parsing GTF file: /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.gtf -parsing /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] [90000] [100000] [110000] [120000] [130000] [140000] [150000] [160000] [170000] [180000] [190000] [200000] [210000] [220000] [230000] [240000] [250000] [260000] [270000] [280000] [290000] [300000] [310000] [320000] [330000] [340000] [350000] [360000] [370000] [380000] [390000] [400000] [410000] [420000] [430000] [440000] [450000] [460000] [470000] [480000] [490000] [500000] [510000] [520000] [530000] [540000] [550000] [560000] [570000] [580000] [590000] [600000] [610000] [620000] [630000] [640000] [650000] [660000] [670000] [680000] [690000] [700000] [710000] [720000] [730000] [740000] [750000] [760000] [770000] [780000] [790000] [800000] [810000] [820000] [830000] [840000] [850000] [860000] [870000] [880000] [890000] [900000] [910000] [920000] [930000] [940000] [950000] [960000] [970000] [980000] [990000] [1000000] [1010000] [1020000] [1030000] [1040000] [1050000] [1060000] -done parsing /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { 'num genes matched < 2' => 15, 'low complexity anchor region' => 12, 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 166, 'num_hits: 4 != num_counted_on_fusion_contigs 3 ' => 6, 'excessive soft clipping' => 5852, 'seq-similar region overlap' => 8, 'per_id < 96' => 4372, ' ** passed ** ' => 2821, 'num_hits: 4 != num_counted_on_fusion_contigs 1 ' => 1, 'exons hit < 2' => 4, 'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 9 }; -writing fusion junction support info. Execution Time = 0.57 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/get_fusion_JUNCTION_reads_from_fusion_contig_bam.pl --gtf_file /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.gtf --MIN_ALIGN_PER_ID 96 --bam /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam --genome_lib_dir ctat_genome_lib_build_dir > /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/get_fusion_SPANNING_reads_from_bam.from_chim_summary.pl --gtf_file /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.gtf --MIN_ALIGN_PER_ID 96 --bam /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam --junction_info /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_junction_info --genome_lib_dir ctat_genome_lib_build_dir > /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_span_reads.sam -outputting the spanning read info: /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. 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[124000] [125000] [126000] [127000] -fusion SPANNING read extraction for scaff: ACACA--LGMN [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] [103000] [104000] [105000] -fusion SPANNING read extraction for scaff: AP000873.1--PCF11 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] -fusion SPANNING read extraction for scaff: CLTA--RASA2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] -fusion SPANNING read extraction for scaff: LINC01002--ZCCHC7 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] 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[140000] [141000] [142000] [143000] [144000] [145000] [146000] [147000] [148000] [149000] [150000] [151000] [152000] [153000] [154000] [155000] [156000] [157000] [158000] [159000] [160000] [161000] [162000] [163000] [164000] [165000] [166000] [167000] [168000] [169000] [170000] [171000] [172000] [173000] [174000] [175000] [176000] [177000] [178000] [179000] [180000] [181000] [182000] [183000] [184000] [185000] [186000] [187000] [188000] [189000] [190000] [191000] [192000] [193000] [194000] [195000] [196000] [197000] [198000] [199000] [200000] [201000] [202000] [203000] [204000] [205000] [206000] [207000] [208000] [209000] [210000] [211000] [212000] [213000] [214000] [215000] [216000] [217000] [218000] [219000] [220000] [221000] [222000] [223000] [224000] [225000] [226000] [227000] [228000] -fusion SPANNING read extraction for scaff: NCOA1--RBKS [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] -fusion SPANNING read extraction for scaff: PCDHA4--PCDHAC1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] -fusion SPANNING read extraction for scaff: PCDHA4--PCDHAC2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] -fusion SPANNING read extraction for scaff: PCDHA4--PCDHA13 [1000] [2000] [3000] [4000] [5000] [6000] -fusion SPANNING read extraction for scaff: PPIL3--CFLAR-AS1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] -fusion SPANNING read extraction for scaff: PUM2--B3GNTL1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] [103000] [104000] [105000] [106000] [107000] [108000] [109000] [110000] [111000] [112000] [113000] [114000] [115000] [116000] [117000] [118000] [119000] [120000] [121000] [122000] [123000] [124000] [125000] [126000] [127000] [128000] [129000] [130000] [131000] [132000] [133000] [134000] [135000] [136000] [137000] [138000] [139000] [140000] [141000] [142000] [143000] [144000] [145000] [146000] [147000] [148000] [149000] [150000] [151000] [152000] [153000] [154000] [155000] [156000] [157000] [158000] [159000] [160000] [161000] [162000] [163000] [164000] [165000] [166000] [167000] [168000] [169000] [170000] [171000] [172000] [173000] [174000] [175000] [176000] [177000] [178000] [179000] [180000] [181000] [182000] [183000] [184000] [185000] [186000] [187000] [188000] [189000] [190000] [191000] [192000] [193000] [194000] [195000] [196000] [197000] [198000] [199000] [200000] [201000] [202000] [203000] [204000] [205000] [206000] [207000] [208000] [209000] -fusion SPANNING read extraction for scaff: ST14--ARHGAP32 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] -fusion SPANNING read extraction for scaff: TLK2--AC240565.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] -fusion SPANNING read extraction for scaff: ZMYND8--GSG1L -filtered reads reasons: $VAR1 = { 'seq similar region alignment' => 5250, 'lacks exon overlap' => 5759 }; Execution Time = 1.54 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/get_fusion_SPANNING_reads_from_bam.from_chim_summary.pl --gtf_file /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.gtf --MIN_ALIGN_PER_ID 96 --bam /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam --junction_info /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_junction_info --genome_lib_dir ctat_genome_lib_build_dir > /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_span_reads.sam Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/coalesce_junction_and_spanning_info.pl /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_junction_info /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info 1 > /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/coalesce_junction_and_spanning_info.pl /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_junction_info /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info 1 > /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/coalesce_junc_n_span.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_EM_runner.pl /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary > /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj EM: Starting log likelihood: -3754.731252 EM: Round [1] log likelihood: -3753.930595 EM: Round [2] log likelihood: -3753.921951 EM: Round [3] log likelihood: -3753.914001 EM: Round [4] log likelihood: -3753.906251 EM: Round [5] log likelihood: -3753.898693 EM: Round [6] log likelihood: -3753.891321 EM: Round [7] log likelihood: -3753.884128 EM: Round [8] log likelihood: -3753.877109 EM: Round [9] log likelihood: -3753.870259 EM: Round [10] log likelihood: -3753.863572 EM: Round [11] log likelihood: -3753.857044 EM: Round [12] log likelihood: -3753.850670 EM: Round [13] log likelihood: -3753.844445 EM: Round [14] log likelihood: -3753.838364 EM: Round [15] log likelihood: -3753.832425 EM: Round [16] log likelihood: -3753.826622 EM: Round [17] log likelihood: -3753.820952 EM: Round [18] log likelihood: -3753.815410 EM: Round [19] log likelihood: -3753.809994 EM: Round [20] log likelihood: -3753.804700 EM: Round [21] log likelihood: -3753.799524 EM: Round [22] log likelihood: -3753.794463 EM: Round [23] log likelihood: -3753.789515 EM: Round [24] log likelihood: -3753.784675 EM: Round [25] log likelihood: -3753.779942 EM: Round [26] log likelihood: -3753.775312 EM: Round [27] log likelihood: -3753.770783 EM: Round [28] log likelihood: -3753.766351 EM: Round [29] log likelihood: -3753.762015 EM: Round [30] log likelihood: -3753.757772 EM: Round [31] log likelihood: -3753.753620 EM: Round [32] log likelihood: -3753.749556 EM: Round [33] log likelihood: -3753.745578 EM: Round [34] log likelihood: -3753.741684 EM: Round [35] log likelihood: -3753.737872 EM: Round [36] log likelihood: -3753.734140 EM: Round [37] log likelihood: -3753.730486 EM: Round [38] log likelihood: -3753.726907 EM: Round [39] log likelihood: -3753.723403 EM: Round [40] log likelihood: -3753.719971 EM: Round [41] log likelihood: -3753.716609 EM: Round [42] log likelihood: -3753.713317 EM: Round [43] log likelihood: -3753.710092 EM: Round [44] log likelihood: -3753.706932 EM: Round [45] log likelihood: -3753.703837 EM: Round [46] log likelihood: -3753.700805 EM: Round [47] log likelihood: -3753.697833 EM: Round [48] log likelihood: -3753.694922 EM: Round [49] log likelihood: -3753.692069 EM: Round [50] log likelihood: -3753.689273 EM: Round [51] log likelihood: -3753.686532 EM: Round [52] log likelihood: -3753.683847 EM: Round [53] log likelihood: -3753.681214 EM: Round [54] log likelihood: -3753.678634 EM: Round [55] log likelihood: -3753.676105 EM: Round [56] log likelihood: -3753.673625 EM: Round [57] log likelihood: -3753.671195 EM: Round [58] log likelihood: -3753.668811 EM: Round [59] log likelihood: -3753.666475 EM: Round [60] log likelihood: -3753.664184 EM: Round [61] log likelihood: -3753.661938 EM: Round [62] log likelihood: -3753.659735 EM: Round [63] log likelihood: -3753.657575 EM: Round [64] log likelihood: -3753.655457 EM: Round [65] log likelihood: -3753.653380 EM: Round [66] log likelihood: -3753.651342 EM: Round [67] log likelihood: -3753.649344 EM: Round [68] log likelihood: -3753.647385 EM: Round [69] log likelihood: -3753.645462 EM: Round [70] log likelihood: -3753.643577 EM: Round [71] log likelihood: -3753.641727 EM: Round [72] log likelihood: -3753.639913 EM: Round [73] log likelihood: -3753.638133 EM: Round [74] log likelihood: -3753.636387 EM: Round [75] log likelihood: -3753.634674 EM: Round [76] log likelihood: -3753.632993 EM: Round [77] log likelihood: -3753.631344 EM: Round [78] log likelihood: -3753.629726 EM: Round [79] log likelihood: -3753.628139 EM: Round [80] log likelihood: -3753.626581 EM: Round [81] log likelihood: -3753.625053 EM: Round [82] log likelihood: -3753.623553 EM: Round [83] log likelihood: -3753.622082 EM: Round [84] log likelihood: -3753.620637 EM: Round [85] log likelihood: -3753.619220 EM: Round [86] log likelihood: -3753.617829 EM: Round [87] log likelihood: -3753.616464 EM: Round [88] log likelihood: -3753.615124 EM: Round [89] log likelihood: -3753.613810 EM: Round [90] log likelihood: -3753.612519 EM: Round [91] log likelihood: -3753.611252 EM: Round [92] log likelihood: -3753.610009 EM: Round [93] log likelihood: -3753.608789 EM: Round [94] log likelihood: -3753.607591 EM: Round [95] log likelihood: -3753.606415 EM: Round [96] log likelihood: -3753.605261 EM: Round [97] log likelihood: -3753.604128 EM: Round [98] log likelihood: -3753.603016 EM: Round [99] log likelihood: -3753.601924 EM: Round [100] log likelihood: -3753.600853 EM: Round [101] log likelihood: -3753.599800 EM: Round [102] log likelihood: -3753.598768 EM: Round [103] log likelihood: -3753.597753 EM: Round [104] log likelihood: -3753.596758 EM: Round [105] log likelihood: -3753.595780 EM: Round [106] log likelihood: -3753.594821 EM: Round [107] log likelihood: -3753.593878 EM: Round [108] log likelihood: -3753.592953 EM: Round [109] log likelihood: -3753.592045 EM: Round [110] log likelihood: -3753.591153 EM: Round [111] log likelihood: -3753.590277 EM: Round [112] log likelihood: -3753.589417 EM: Round [113] log likelihood: -3753.588573 EM: Round [114] log likelihood: -3753.587744 EM: Round [115] log likelihood: -3753.586929 EM: Round [116] log likelihood: -3753.586130 EM: Round [117] log likelihood: -3753.585345 EM: Round [118] log likelihood: -3753.584574 EM: Round [119] log likelihood: -3753.583817 EM: Round [120] log likelihood: -3753.583073 EM: Round [121] log likelihood: -3753.582343 EM: Round [122] log likelihood: -3753.581626 EM: Round [123] log likelihood: -3753.580922 EM: Round [124] log likelihood: -3753.580231 EM: Round [125] log likelihood: -3753.579552 EM: Round [126] log likelihood: -3753.578885 EM: Round [127] log likelihood: -3753.578230 EM: Round [128] log likelihood: -3753.577586 EM: Round [129] log likelihood: -3753.576954 EM: Round [130] log likelihood: -3753.576334 EM: Round [131] log likelihood: -3753.575724 EM: Round [132] log likelihood: -3753.575126 EM: Round [133] log likelihood: -3753.574538 EM: Round [134] log likelihood: -3753.573960 EM: Round [135] log likelihood: -3753.573393 EM: Round [136] log likelihood: -3753.572836 EM: Round [137] log likelihood: -3753.572289 EM: Round [138] log likelihood: -3753.571752 EM: Round [139] log likelihood: -3753.571224 EM: Round [140] log likelihood: -3753.570705 EM: Round [141] log likelihood: -3753.570196 EM: Round [142] log likelihood: -3753.569696 EM: Round [143] log likelihood: -3753.569204 EM: Round [144] log likelihood: -3753.568721 EM: Round [145] log likelihood: -3753.568247 EM: Round [146] log likelihood: -3753.567781 EM: Round [147] log likelihood: -3753.567324 EM: Round [148] log likelihood: -3753.566875 EM: Round [149] log likelihood: -3753.566433 EM: Round [150] log likelihood: -3753.565999 EM: Round [151] log likelihood: -3753.565573 EM: Round [152] log likelihood: -3753.565155 EM: Round [153] log likelihood: -3753.564744 EM: Round [154] log likelihood: -3753.564340 EM: Round [155] log likelihood: -3753.563943 EM: Round [156] log likelihood: -3753.563553 EM: Round [157] log likelihood: -3753.563170 EM: Round [158] log likelihood: -3753.562794 EM: Round [159] log likelihood: -3753.562425 EM: Round [160] log likelihood: -3753.562062 EM: Round [161] log likelihood: -3753.561705 EM: Round [162] log likelihood: -3753.561355 EM: Round [163] log likelihood: -3753.561011 EM: Round [164] log likelihood: -3753.560672 EM: Round [165] log likelihood: -3753.560340 EM: Round [166] log likelihood: -3753.560014 EM: Round [167] log likelihood: -3753.559693 EM: Round [168] log likelihood: -3753.559378 EM: Round [169] log likelihood: -3753.559069 EM: Round [170] log likelihood: -3753.558765 EM: Round [171] log likelihood: -3753.558466 EM: Round [172] log likelihood: -3753.558173 EM: Round [173] log likelihood: -3753.557884 EM: Round [174] log likelihood: -3753.557601 EM: Round [175] log likelihood: -3753.557323 EM: Round [176] log likelihood: -3753.557049 EM: Round [177] log likelihood: -3753.556780 EM: Round [178] log likelihood: -3753.556517 EM: Round [179] log likelihood: -3753.556257 EM: Round [180] log likelihood: -3753.556002 EM: Round [181] log likelihood: -3753.555752 EM: Round [182] log likelihood: -3753.555506 EM: Round [183] log likelihood: -3753.555264 EM: Round [184] log likelihood: -3753.555027 EM: Round [185] log likelihood: -3753.554794 EM: Round [186] log likelihood: -3753.554564 EM: Round [187] log likelihood: -3753.554339 EM: Round [188] log likelihood: -3753.554118 EM: Round [189] log likelihood: -3753.553901 EM: Round [190] log likelihood: -3753.553687 EM: Round [191] log likelihood: -3753.553477 EM: Round [192] log likelihood: -3753.553271 EM: Round [193] log likelihood: -3753.553068 EM: Round [194] log likelihood: -3753.552869 EM: Round [195] log likelihood: -3753.552673 EM: Round [196] log likelihood: -3753.552481 EM: Round [197] log likelihood: -3753.552292 EM: Round [198] log likelihood: -3753.552107 EM: Round [199] log likelihood: -3753.551924 EM: Round [200] log likelihood: -3753.551745 EM: Round [201] log likelihood: -3753.551569 EM: Round [202] log likelihood: -3753.551396 EM: Round [203] log likelihood: -3753.551226 EM: Round [204] log likelihood: -3753.551059 EM: Round [205] log likelihood: -3753.550895 EM: Round [206] log likelihood: -3753.550734 EM: Round [207] log likelihood: -3753.550575 EM: Round [208] log likelihood: -3753.550420 EM: Round [209] log likelihood: -3753.550267 EM: Round [210] log likelihood: -3753.550116 EM: Round [211] log likelihood: -3753.549969 EM: Round [212] log likelihood: -3753.549823 EM: Round [213] log likelihood: -3753.549681 EM: Round [214] log likelihood: -3753.549541 EM: Round [215] log likelihood: -3753.549403 EM: Round [216] log likelihood: -3753.549268 EM: Round [217] log likelihood: -3753.549135 EM: Round [218] log likelihood: -3753.549004 EM: Round [219] log likelihood: -3753.548876 EM: Round [220] log likelihood: -3753.548750 EM: Round [221] log likelihood: -3753.548626 EM: Round [222] log likelihood: -3753.548504 EM: Round [223] log likelihood: -3753.548384 EM: Round [224] log likelihood: -3753.548266 EM: Round [225] log likelihood: -3753.548151 EM: Round [226] log likelihood: -3753.548037 EM: Round [227] log likelihood: -3753.547926 EM: Round [228] log likelihood: -3753.547816 EM: Round [229] log likelihood: -3753.547708 EM: Round [230] log likelihood: -3753.547602 EM: Round [231] log likelihood: -3753.547498 EM: Round [232] log likelihood: -3753.547396 EM: Round [233] log likelihood: -3753.547296 EM: Round [234] log likelihood: -3753.547197 EM: Stopping iterations at round 234 due to insufficient improvement in likelihood. Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_EM_runner.pl /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary > /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/init_EM_adj_counts.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/filter_fusions_by_frag_thresholds.pl --min_junction_reads 0 --min_sum_frags 1 --min_novel_junction_support 3 --min_spanning_frags_only 5 --fusion_preds /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj --require_LDAS 1 > /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/filter_fusions_by_frag_thresholds.pl --min_junction_reads 0 --min_sum_frags 1 --min_novel_junction_support 3 --min_spanning_frags_only 5 --fusion_preds /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj --require_LDAS 1 > /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/filter_by_frag_threshs.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/column_extractions.pl /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary LeftGene,RightGene,JunctionReads > /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.fusion_junction_read_accs Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/column_extractions.pl /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary LeftGene,RightGene,JunctionReads > /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.fusion_junction_read_accs Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/prep_igv_extract_junc_reads.ok Running: bash -c "set -eof pipefail; /usr/local/src/STAR-Fusion/FusionInspector/util/retrieve_fusion_junction_reads_by_accession.pl /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.fusion_junction_read_accs /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam | samtools view -@ 8 -bT /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.fa - | samtools sort -@ 8 - -o /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.junction_reads.bam" [bam_sort_core] merging from 0 files and 8 in-memory blocks... Execution Time = 0.00 minutes. CMD: bash -c "set -eof pipefail; /usr/local/src/STAR-Fusion/FusionInspector/util/retrieve_fusion_junction_reads_by_accession.pl /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.fusion_junction_read_accs /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam | samtools view -@ 8 -bT /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.fa - | samtools sort -@ 8 - -o /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.junction_reads.bam" Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/prep_igv_junc_reads_bam.ok Running: samtools index /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.junction_reads.bam Execution Time = 0.00 minutes. CMD: samtools index /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.junction_reads.bam Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/samtools_idx_junc_reads_bam.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_pair_to_bed.pl /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.junction_reads.bam > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.junction_reads.bam.bed Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_pair_to_bed.pl /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.junction_reads.bam > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.junction_reads.bam.bed Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/tih_rna_sample_00669_B23MVV7LT4_1.junction_reads.bam.bam_to_bed.ok Running: sort -k1,1 -k2,2n /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.junction_reads.bam.bed > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.junction_reads.bam.bed.sorted.bed Execution Time = 0.00 minutes. CMD: sort -k1,1 -k2,2n /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.junction_reads.bam.bed > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.junction_reads.bam.bed.sorted.bed Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/tih_rna_sample_00669_B23MVV7LT4_1.junction_reads.bam.bedsort.ok Running: bgzip -f /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.junction_reads.bam.bed.sorted.bed Execution Time = 0.00 minutes. CMD: bgzip -f /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.junction_reads.bam.bed.sorted.bed Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/tih_rna_sample_00669_B23MVV7LT4_1.junction_reads.bam.bgzip.ok Running: tabix -p bed /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.junction_reads.bam.bed.sorted.bed.gz Execution Time = 0.00 minutes. CMD: tabix -p bed /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.junction_reads.bam.bed.sorted.bed.gz Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/tih_rna_sample_00669_B23MVV7LT4_1.junction_reads.bam.tabix.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/column_extractions.pl /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary LeftGene,RightGene,SpanningFrags > /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.fusion_spanning_read_accs Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/column_extractions.pl /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary LeftGene,RightGene,SpanningFrags > /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.fusion_spanning_read_accs Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/span_reads_acc.ok Running: samtools view -H /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam -b -o /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam Execution Time = 0.00 minutes. CMD: samtools view -H /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam -b -o /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/init_spanning_reads_bam.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/retrieve_fusion_spanning_reads_by_accession.pl /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.fusion_spanning_read_accs /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_span_reads.sam | samtools view -@ 8 -bT /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.fa - | samtools sort -@ 8 - -o /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam || : Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/retrieve_fusion_spanning_reads_by_accession.pl /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.fusion_spanning_read_accs /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam.fusion_span_reads.sam | samtools view -@ 8 -bT /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.fa - | samtools sort -@ 8 - -o /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam || : Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/prep_spanning_reads.ok Running: samtools index /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam Execution Time = 0.00 minutes. CMD: samtools index /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/samtools_index_span_reads_bam.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_pair_to_bed.pl /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam.bed Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_pair_to_bed.pl /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam.bed Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam.bam_to_bed.ok Running: sort -k1,1 -k2,2n /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam.bed > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam.bed.sorted.bed Execution Time = 0.00 minutes. CMD: sort -k1,1 -k2,2n /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam.bed > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam.bed.sorted.bed Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam.bedsort.ok Running: bgzip -f /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam.bed.sorted.bed Execution Time = 0.00 minutes. CMD: bgzip -f /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam.bed.sorted.bed Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam.bgzip.ok Running: tabix -p bed /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam.bed.sorted.bed.gz Execution Time = 0.00 minutes. CMD: tabix -p bed /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam.bed.sorted.bed.gz Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/tih_rna_sample_00669_B23MVV7LT4_1.spanning_reads.bam.tabix.ok Running: cp /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.consolidated.bam Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.star.cSorted.dupsMarked.bam /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.consolidated.bam Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/cp_consol_bam.ok Running: samtools index /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.consolidated.bam Execution Time = 0.01 minutes. CMD: samtools index /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.consolidated.bam Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/index_consol_bam.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_to_frag_coords.pl --sam /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.consolidated.bam --max_insert_size 10000000 -extracting read coordinates from /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.consolidated.bam into /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.consolidated.bam.read_coords CMD: touch /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.consolidated.bam.read_coords > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.consolidated.bam.frag_coords > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.consolidated.bam.frag_coords.coord_sorted Execution Time = 0.27 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_to_frag_coords.pl --sam /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.consolidated.bam --max_insert_size 10000000 Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/prep_igv_sam_frag_coordss.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_summary_to_igv_JuncSpan_fmt.pl /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.consolidated.bam.frag_coords > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.igv.FusionJuncSpan Execution Time = 0.01 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_summary_to_igv_JuncSpan_fmt.pl /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.consolidated.bam.frag_coords > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.igv.FusionJuncSpan Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/prep_igv_juncspan_fmt.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/get_pfam_domain_info.pl --finspector_gtf /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.gtf --genome_lib_dir ctat_genome_lib_build_dir > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.igv.Pfam.gff3 Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/get_pfam_domain_info.pl --finspector_gtf /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.gtf --genome_lib_dir ctat_genome_lib_build_dir > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.igv.Pfam.gff3 Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/prep_igv_pfam_gff3.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/transcript_gff3_to_bed.pl /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.igv.Pfam.gff3 > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.igv.Pfam.bed Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/transcript_gff3_to_bed.pl /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.igv.Pfam.gff3 > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.igv.Pfam.bed Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/prep_igv_pfam_bed.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/get_seq_similar_region_FI_coordinates.pl --finspector_gtf /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.gtf --genome_lib_dir ctat_genome_lib_build_dir > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.igv.seqsimilar.gff3 Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/get_seq_similar_region_FI_coordinates.pl --finspector_gtf /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.gtf --genome_lib_dir ctat_genome_lib_build_dir > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.igv.seqsimilar.gff3 Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/prep_igv_seqsim_gff3.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/transcript_gff3_to_bed.pl /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.igv.seqsimilar.gff3 > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.igv.seqsimilar.bed Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/transcript_gff3_to_bed.pl /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.igv.seqsimilar.gff3 > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.igv.seqsimilar.bed Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/prep_igv_seqsim_bed.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/append_breakpoint_junction_info_via_FI_contigs.pl /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.fa > /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/append_breakpoint_junction_info_via_FI_contigs.pl /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.fa > /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/add_splice_info.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/../FusionFilter/blast_and_promiscuity_filter.pl --fusion_preds /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --out_prefix /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1 --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.post_blast_and_promiscuity_filter Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/../FusionFilter/blast_and_promiscuity_filter.pl --fusion_preds /tmp/nxf.ZB9XBvOyXZ/fi_workdir/tih_rna_sample_00669_B23MVV7LT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --out_prefix /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1 --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/blast_filter.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/../FusionFilter/util/filter_by_annotation_rules.pl --fusions /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.post_blast_and_promiscuity_filter --genome_lib_dir /tmp/nxf.ZB9XBvOyXZ/ctat_genome_lib_build_dir -done, see /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/../FusionFilter/util/filter_by_annotation_rules.pl --fusions /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.post_blast_and_promiscuity_filter --genome_lib_dir /tmp/nxf.ZB9XBvOyXZ/ctat_genome_lib_build_dir Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/annot_filter.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_EM_runner.pl /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj EM: Starting log likelihood: -3607.690341 EM: Round [1] log likelihood: -3607.065249 EM: Round [2] log likelihood: -3607.064724 EM: Round [3] log likelihood: -3607.064722 EM: Stopping iterations at round 3 due to insufficient improvement in likelihood. Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_EM_runner.pl /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/EM_adj_counts.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/incorporate_FFPM_into_final_report.pl /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1_1.fastp.fastq.gz /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM -total frags in /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1_1.fastp.fastq.gz: 272885379 Execution Time = 5.09 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/incorporate_FFPM_into_final_report.pl /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1_1.fastp.fastq.gz /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/add_FFPM.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/misc/find_microhomologies_by_kmer_matches.pl --fasta /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.fa --gtf /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.gtf > /tmp/nxf.ZB9XBvOyXZ/fi_workdir/microH.dat Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/misc/find_microhomologies_by_kmer_matches.pl --fasta /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.fa --gtf /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.gtf > /tmp/nxf.ZB9XBvOyXZ/fi_workdir/microH.dat Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/microH.dat.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/misc/append_microH_distance.py /tmp/nxf.ZB9XBvOyXZ/fi_workdir/microH.dat /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM.wMicroH Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/misc/append_microH_distance.py /tmp/nxf.ZB9XBvOyXZ/fi_workdir/microH.dat /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM.wMicroH Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/append_microH_info.ok Running: cp /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM.wMicroH /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.FusionInspector.fusions.tsv Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM.wMicroH /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.FusionInspector.fusions.tsv Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/cp_final.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/column_exclusions.pl /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.FusionInspector.fusions.tsv JunctionReads,SpanningFrags,CounterFusionLeftReads,CounterFusionRightReads > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.FusionInspector.fusions.abridged.tsv Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/column_exclusions.pl /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.FusionInspector.fusions.tsv JunctionReads,SpanningFrags,CounterFusionLeftReads,CounterFusionRightReads > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.FusionInspector.fusions.abridged.tsv Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/final.abridged.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/util/create_fusion_inspector_igvjs.py --fusion_inspector_directory /tmp/nxf.ZB9XBvOyXZ --json_outfile /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.fusion_inspector_web.json --file_prefix tih_rna_sample_00669_B23MVV7LT4_1 Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/create_fusion_inspector_igvjs.py --fusion_inspector_directory /tmp/nxf.ZB9XBvOyXZ --json_outfile /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.fusion_inspector_web.json --file_prefix tih_rna_sample_00669_B23MVV7LT4_1 Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/create_fi_igvjs.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/fusion-reports/create_fusion_report.py --html_template /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_report_html_template/igvjs_fusion.html --fusions_json /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.fusion_inspector_web.json --input_file_prefix tih_rna_sample_00669_B23MVV7LT4_1 --html_output /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.fusion_inspector_web.html Warning - not locating file: /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.gmap_trinity_GG.fusions.gff3.bed Execution Time = 0.01 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/fusion-reports/create_fusion_report.py --html_template /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_report_html_template/igvjs_fusion.html --fusions_json /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.fusion_inspector_web.json --input_file_prefix tih_rna_sample_00669_B23MVV7LT4_1 --html_output /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.fusion_inspector_web.html Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/fusion_reports_html.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/FusionAnnotator/FusionAnnotator --annotate /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.FusionInspector.fusions.abridged.tsv --genome_lib_dir /tmp/nxf.ZB9XBvOyXZ/ctat_genome_lib_build_dir > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.FusionInspector.fusions.abridged.tsv.annotated Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionAnnotator/FusionAnnotator --annotate /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.FusionInspector.fusions.abridged.tsv --genome_lib_dir /tmp/nxf.ZB9XBvOyXZ/ctat_genome_lib_build_dir > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.FusionInspector.fusions.abridged.tsv.annotated Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/fusion_annotator.ok Running: /usr/local/src/STAR-Fusion/FusionInspector/FusionAnnotator/util/fusion_to_coding_region_effect.pl --fusions /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.FusionInspector.fusions.abridged.tsv.annotated --genome_lib_dir /tmp/nxf.ZB9XBvOyXZ/ctat_genome_lib_build_dir > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.FusionInspector.fusions.abridged.tsv.annotated.coding_effect WARNING, no entry stored in dbm for [ENSG00000280136.2] WARNING, no entry stored in dbm for [ENSG00000247137.9] WARNING, no entry stored in dbm for [ENSG00000247137.9] WARNING, no entry stored in dbm for [ENSG00000226049.3] WARNING, no entry stored in dbm for [ENSG00000260404.3] WARNING, no entry stored in dbm for [ENSG00000282508.2] WARNING, no entry stored in dbm for [ENSG00000226312.7] WARNING, no entry stored in dbm for [ENSG00000226312.7] Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionAnnotator/util/fusion_to_coding_region_effect.pl --fusions /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.FusionInspector.fusions.abridged.tsv.annotated --genome_lib_dir /tmp/nxf.ZB9XBvOyXZ/ctat_genome_lib_build_dir > /tmp/nxf.ZB9XBvOyXZ/tih_rna_sample_00669_B23MVV7LT4_1.FusionInspector.fusions.abridged.tsv.annotated.coding_effect Running: touch /tmp/nxf.ZB9XBvOyXZ/chckpts_dir/fusion_coding_region_effect.ok ls: cannot access 'IGV_inputs': No such file or directory