[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00575_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (3533594) splicing reads. 155659 splicing junctions. 137855 known splicing junctions. 17804 novel splicing junctions.
sampling 10% (7067188) splicing reads. 174555 splicing junctions. 148686 known splicing junctions. 25869 novel splicing junctions.
sampling 15% (10600783) splicing reads. 184086 splicing junctions. 153186 known splicing junctions. 30900 novel splicing junctions.
sampling 20% (14134377) splicing reads. 189871 splicing junctions. 155661 known splicing junctions. 34210 novel splicing junctions.
sampling 25% (17667972) splicing reads. 193876 splicing junctions. 157249 known splicing junctions. 36627 novel splicing junctions.
sampling 30% (21201566) splicing reads. 196848 splicing junctions. 158423 known splicing junctions. 38425 novel splicing junctions.
sampling 35% (24735161) splicing reads. 199102 splicing junctions. 159225 known splicing junctions. 39877 novel splicing junctions.
sampling 40% (28268755) splicing reads. 200922 splicing junctions. 159899 known splicing junctions. 41023 novel splicing junctions.
sampling 45% (31802350) splicing reads. 202285 splicing junctions. 160341 known splicing junctions. 41944 novel splicing junctions.
sampling 50% (35335944) splicing reads. 203452 splicing junctions. 160734 known splicing junctions. 42718 novel splicing junctions.
sampling 55% (38869538) splicing reads. 204428 splicing junctions. 161021 known splicing junctions. 43407 novel splicing junctions.
sampling 60% (42403133) splicing reads. 205231 splicing junctions. 161265 known splicing junctions. 43966 novel splicing junctions.
sampling 65% (45936727) splicing reads. 205898 splicing junctions. 161447 known splicing junctions. 44451 novel splicing junctions.
sampling 70% (49470322) splicing reads. 206470 splicing junctions. 161622 known splicing junctions. 44848 novel splicing junctions.
sampling 75% (53003916) splicing reads. 206957 splicing junctions. 161766 known splicing junctions. 45191 novel splicing junctions.
sampling 80% (56537511) splicing reads. 207311 splicing junctions. 161882 known splicing junctions. 45429 novel splicing junctions.
sampling 85% (60071105) splicing reads. 207590 splicing junctions. 161972 known splicing junctions. 45618 novel splicing junctions.
sampling 90% (63604700) splicing reads. 207808 splicing junctions. 162035 known splicing junctions. 45773 novel splicing junctions.
sampling 95% (67138294) splicing reads. 207926 splicing junctions. 162079 known splicing junctions. 45847 novel splicing junctions.
sampling 100% (70671889) splicing reads. 207993 splicing junctions. 162096 known splicing junctions. 45897 novel splicing junctions.