[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00570_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (18242208) splicing reads. 275593 splicing junctions. 184262 known splicing junctions. 91331 novel splicing junctions.
sampling 10% (36484416) splicing reads. 346468 splicing junctions. 196871 known splicing junctions. 149597 novel splicing junctions.
sampling 15% (54726625) splicing reads. 398475 splicing junctions. 203568 known splicing junctions. 194907 novel splicing junctions.
sampling 20% (72968833) splicing reads. 439733 splicing junctions. 208017 known splicing junctions. 231716 novel splicing junctions.
sampling 25% (91211042) splicing reads. 473878 splicing junctions. 211214 known splicing junctions. 262664 novel splicing junctions.
sampling 30% (109453250) splicing reads. 502674 splicing junctions. 213689 known splicing junctions. 288985 novel splicing junctions.
sampling 35% (127695458) splicing reads. 527283 splicing junctions. 215583 known splicing junctions. 311700 novel splicing junctions.
sampling 40% (145937667) splicing reads. 548545 splicing junctions. 217149 known splicing junctions. 331396 novel splicing junctions.
sampling 45% (164179875) splicing reads. 567028 splicing junctions. 218386 known splicing junctions. 348642 novel splicing junctions.
sampling 50% (182422084) splicing reads. 583133 splicing junctions. 219499 known splicing junctions. 363634 novel splicing junctions.
sampling 55% (200664292) splicing reads. 597185 splicing junctions. 220431 known splicing junctions. 376754 novel splicing junctions.
sampling 60% (218906500) splicing reads. 609690 splicing junctions. 221187 known splicing junctions. 388503 novel splicing junctions.
sampling 65% (237148709) splicing reads. 620538 splicing junctions. 221880 known splicing junctions. 398658 novel splicing junctions.
sampling 70% (255390917) splicing reads. 629955 splicing junctions. 222417 known splicing junctions. 407538 novel splicing junctions.
sampling 75% (273633126) splicing reads. 638062 splicing junctions. 222908 known splicing junctions. 415154 novel splicing junctions.
sampling 80% (291875334) splicing reads. 644901 splicing junctions. 223324 known splicing junctions. 421577 novel splicing junctions.
sampling 85% (310117542) splicing reads. 650590 splicing junctions. 223624 known splicing junctions. 426966 novel splicing junctions.
sampling 90% (328359751) splicing reads. 655380 splicing junctions. 223901 known splicing junctions. 431479 novel splicing junctions.
sampling 95% (346601959) splicing reads. 659103 splicing junctions. 224123 known splicing junctions. 434980 novel splicing junctions.
sampling 100% (364844168) splicing reads. 661884 splicing junctions. 224278 known splicing junctions. 437606 novel splicing junctions.