Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/71/3a2641bc9d8f643695e473b5e1e05f/tih_rna_sample_00572_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f8/e88fd7fc7045f143707b3a331bf8bc/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/35/e2dcc39862e0d9325d663578f69a52/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/35/e2dcc39862e0d9325d663578f69a52/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ef/d3c4889fac8bddf20c6ee2cee3691b/tih_rna_sample_00572_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00572_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (11409455) splicing reads. 250820 splicing junctions. 185409 known splicing junctions. 65411 novel splicing junctions.
sampling 10% (22818910) splicing reads. 301818 splicing junctions. 198310 known splicing junctions. 103508 novel splicing junctions.
sampling 15% (34228365) splicing reads. 336343 splicing junctions. 204996 known splicing junctions. 131347 novel splicing junctions.
sampling 20% (45637821) splicing reads. 362712 splicing junctions. 209277 known splicing junctions. 153435 novel splicing junctions.
sampling 25% (57047276) splicing reads. 384146 splicing junctions. 212361 known splicing junctions. 171785 novel splicing junctions.
sampling 30% (68456731) splicing reads. 401462 splicing junctions. 214742 known splicing junctions. 186720 novel splicing junctions.
sampling 35% (79866186) splicing reads. 416078 splicing junctions. 216570 known splicing junctions. 199508 novel splicing junctions.
sampling 40% (91275642) splicing reads. 428595 splicing junctions. 218076 known splicing junctions. 210519 novel splicing junctions.
sampling 45% (102685097) splicing reads. 439237 splicing junctions. 219342 known splicing junctions. 219895 novel splicing junctions.
sampling 50% (114094552) splicing reads. 448393 splicing junctions. 220368 known splicing junctions. 228025 novel splicing junctions.
sampling 55% (125504007) splicing reads. 456415 splicing junctions. 221276 known splicing junctions. 235139 novel splicing junctions.
sampling 60% (136913463) splicing reads. 463211 splicing junctions. 221988 known splicing junctions. 241223 novel splicing junctions.
sampling 65% (148322918) splicing reads. 469261 splicing junctions. 222624 known splicing junctions. 246637 novel splicing junctions.
sampling 70% (159732373) splicing reads. 474431 splicing junctions. 223114 known splicing junctions. 251317 novel splicing junctions.
sampling 75% (171141828) splicing reads. 478915 splicing junctions. 223564 known splicing junctions. 255351 novel splicing junctions.
sampling 80% (182551284) splicing reads. 482701 splicing junctions. 223947 known splicing junctions. 258754 novel splicing junctions.
sampling 85% (193960739) splicing reads. 485945 splicing junctions. 224270 known splicing junctions. 261675 novel splicing junctions.
sampling 90% (205370194) splicing reads. 488626 splicing junctions. 224534 known splicing junctions. 264092 novel splicing junctions.
sampling 95% (216779649) splicing reads. 490730 splicing junctions. 224736 known splicing junctions. 265994 novel splicing junctions.
sampling 100% (228189105) splicing reads. 492244 splicing junctions. 224885 known splicing junctions. 267359 novel splicing junctions.
null device 
          1