[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00592_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (14443969) splicing reads. 257038 splicing junctions. 187583 known splicing junctions. 69455 novel splicing junctions.
sampling 10% (28887939) splicing reads. 309323 splicing junctions. 199922 known splicing junctions. 109401 novel splicing junctions.
sampling 15% (43331909) splicing reads. 344977 splicing junctions. 206365 known splicing junctions. 138612 novel splicing junctions.
sampling 20% (57775879) splicing reads. 371939 splicing junctions. 210395 known splicing junctions. 161544 novel splicing junctions.
sampling 25% (72219849) splicing reads. 393679 splicing junctions. 213325 known splicing junctions. 180354 novel splicing junctions.
sampling 30% (86663819) splicing reads. 411473 splicing junctions. 215546 known splicing junctions. 195927 novel splicing junctions.
sampling 35% (101107788) splicing reads. 426477 splicing junctions. 217293 known splicing junctions. 209184 novel splicing junctions.
sampling 40% (115551758) splicing reads. 439056 splicing junctions. 218643 known splicing junctions. 220413 novel splicing junctions.
sampling 45% (129995728) splicing reads. 449854 splicing junctions. 219756 known splicing junctions. 230098 novel splicing junctions.
sampling 50% (144439698) splicing reads. 458993 splicing junctions. 220681 known splicing junctions. 238312 novel splicing junctions.
sampling 55% (158883668) splicing reads. 467180 splicing junctions. 221462 known splicing junctions. 245718 novel splicing junctions.
sampling 60% (173327638) splicing reads. 474405 splicing junctions. 222127 known splicing junctions. 252278 novel splicing junctions.
sampling 65% (187771608) splicing reads. 480689 splicing junctions. 222775 known splicing junctions. 257914 novel splicing junctions.
sampling 70% (202215577) splicing reads. 486052 splicing junctions. 223249 known splicing junctions. 262803 novel splicing junctions.
sampling 75% (216659547) splicing reads. 490634 splicing junctions. 223666 known splicing junctions. 266968 novel splicing junctions.
sampling 80% (231103517) splicing reads. 494742 splicing junctions. 224030 known splicing junctions. 270712 novel splicing junctions.
sampling 85% (245547487) splicing reads. 498309 splicing junctions. 224339 known splicing junctions. 273970 novel splicing junctions.
sampling 90% (259991457) splicing reads. 501344 splicing junctions. 224581 known splicing junctions. 276763 novel splicing junctions.
sampling 95% (274435427) splicing reads. 503929 splicing junctions. 224801 known splicing junctions. 279128 novel splicing junctions.
sampling 100% (288879397) splicing reads. 505959 splicing junctions. 224977 known splicing junctions. 280982 novel splicing junctions.