[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00583_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (11043870) splicing reads. 245113 splicing junctions. 184012 known splicing junctions. 61101 novel splicing junctions.
sampling 10% (22087740) splicing reads. 288521 splicing junctions. 195257 known splicing junctions. 93264 novel splicing junctions.
sampling 15% (33131610) splicing reads. 316126 splicing junctions. 200950 known splicing junctions. 115176 novel splicing junctions.
sampling 20% (44175480) splicing reads. 335860 splicing junctions. 204443 known splicing junctions. 131417 novel splicing junctions.
sampling 25% (55219350) splicing reads. 350981 splicing junctions. 206874 known splicing junctions. 144107 novel splicing junctions.
sampling 30% (66263220) splicing reads. 362680 splicing junctions. 208607 known splicing junctions. 154073 novel splicing junctions.
sampling 35% (77307091) splicing reads. 372183 splicing junctions. 209990 known splicing junctions. 162193 novel splicing junctions.
sampling 40% (88350961) splicing reads. 379967 splicing junctions. 211059 known splicing junctions. 168908 novel splicing junctions.
sampling 45% (99394831) splicing reads. 386508 splicing junctions. 211940 known splicing junctions. 174568 novel splicing junctions.
sampling 50% (110438701) splicing reads. 391940 splicing junctions. 212661 known splicing junctions. 179279 novel splicing junctions.
sampling 55% (121482571) splicing reads. 396509 splicing junctions. 213250 known splicing junctions. 183259 novel splicing junctions.
sampling 60% (132526441) splicing reads. 400360 splicing junctions. 213723 known splicing junctions. 186637 novel splicing junctions.
sampling 65% (143570311) splicing reads. 403590 splicing junctions. 214073 known splicing junctions. 189517 novel splicing junctions.
sampling 70% (154614182) splicing reads. 406507 splicing junctions. 214417 known splicing junctions. 192090 novel splicing junctions.
sampling 75% (165658052) splicing reads. 408800 splicing junctions. 214698 known splicing junctions. 194102 novel splicing junctions.
sampling 80% (176701922) splicing reads. 410748 splicing junctions. 214934 known splicing junctions. 195814 novel splicing junctions.
sampling 85% (187745792) splicing reads. 412363 splicing junctions. 215124 known splicing junctions. 197239 novel splicing junctions.
sampling 90% (198789662) splicing reads. 413647 splicing junctions. 215282 known splicing junctions. 198365 novel splicing junctions.
sampling 95% (209833532) splicing reads. 414681 splicing junctions. 215416 known splicing junctions. 199265 novel splicing junctions.
sampling 100% (220877403) splicing reads. 415388 splicing junctions. 215491 known splicing junctions. 199897 novel splicing junctions.