[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00594_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (16517889) splicing reads. 264942 splicing junctions. 185779 known splicing junctions. 79163 novel splicing junctions.
sampling 10% (33035778) splicing reads. 324697 splicing junctions. 198692 known splicing junctions. 126005 novel splicing junctions.
sampling 15% (49553667) splicing reads. 367473 splicing junctions. 205687 known splicing junctions. 161786 novel splicing junctions.
sampling 20% (66071556) splicing reads. 401106 splicing junctions. 210403 known splicing junctions. 190703 novel splicing junctions.
sampling 25% (82589445) splicing reads. 428510 splicing junctions. 213665 known splicing junctions. 214845 novel splicing junctions.
sampling 30% (99107334) splicing reads. 452130 splicing junctions. 216222 known splicing junctions. 235908 novel splicing junctions.
sampling 35% (115625223) splicing reads. 472199 splicing junctions. 218266 known splicing junctions. 253933 novel splicing junctions.
sampling 40% (132143112) splicing reads. 489533 splicing junctions. 219938 known splicing junctions. 269595 novel splicing junctions.
sampling 45% (148661001) splicing reads. 504846 splicing junctions. 221351 known splicing junctions. 283495 novel splicing junctions.
sampling 50% (165178890) splicing reads. 518105 splicing junctions. 222501 known splicing junctions. 295604 novel splicing junctions.
sampling 55% (181696779) splicing reads. 530015 splicing junctions. 223488 known splicing junctions. 306527 novel splicing junctions.
sampling 60% (198214668) splicing reads. 540450 splicing junctions. 224361 known splicing junctions. 316089 novel splicing junctions.
sampling 65% (214732557) splicing reads. 549730 splicing junctions. 225070 known splicing junctions. 324660 novel splicing junctions.
sampling 70% (231250446) splicing reads. 557928 splicing junctions. 225691 known splicing junctions. 332237 novel splicing junctions.
sampling 75% (247768335) splicing reads. 565064 splicing junctions. 226181 known splicing junctions. 338883 novel splicing junctions.
sampling 80% (264286224) splicing reads. 571192 splicing junctions. 226629 known splicing junctions. 344563 novel splicing junctions.
sampling 85% (280804113) splicing reads. 576605 splicing junctions. 227027 known splicing junctions. 349578 novel splicing junctions.
sampling 90% (297322002) splicing reads. 581163 splicing junctions. 227352 known splicing junctions. 353811 novel splicing junctions.
sampling 95% (313839891) splicing reads. 585007 splicing junctions. 227616 known splicing junctions. 357391 novel splicing junctions.
sampling 100% (330357780) splicing reads. 588150 splicing junctions. 227832 known splicing junctions. 360318 novel splicing junctions.