Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f8/e88fd7fc7045f143707b3a331bf8bc/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d8/8c30fc00fa3cc29c1e42a20d3242d9/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5c/08bf324c3cf25aebafe8407e410b94/tih_rna_sample_00581_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9b/bee10aa702195de1e8c3898ba03a5a/tih_rna_sample_00581_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d8/8c30fc00fa3cc29c1e42a20d3242d9/.command.run
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00581_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (14377270) splicing reads. 244746 splicing junctions. 177271 known splicing junctions. 67475 novel splicing junctions.
sampling 10% (28754541) splicing reads. 287792 splicing junctions. 187657 known splicing junctions. 100135 novel splicing junctions.
sampling 15% (43131811) splicing reads. 313722 splicing junctions. 192775 known splicing junctions. 120947 novel splicing junctions.
sampling 20% (57509082) splicing reads. 331838 splicing junctions. 195842 known splicing junctions. 135996 novel splicing junctions.
sampling 25% (71886353) splicing reads. 345280 splicing junctions. 197985 known splicing junctions. 147295 novel splicing junctions.
sampling 30% (86263623) splicing reads. 355524 splicing junctions. 199533 known splicing junctions. 155991 novel splicing junctions.
sampling 35% (100640894) splicing reads. 363560 splicing junctions. 200649 known splicing junctions. 162911 novel splicing junctions.
sampling 40% (115018165) splicing reads. 370221 splicing junctions. 201564 known splicing junctions. 168657 novel splicing junctions.
sampling 45% (129395435) splicing reads. 375702 splicing junctions. 202334 known splicing junctions. 173368 novel splicing junctions.
sampling 50% (143772706) splicing reads. 380175 splicing junctions. 202946 known splicing junctions. 177229 novel splicing junctions.
sampling 55% (158149977) splicing reads. 383781 splicing junctions. 203402 known splicing junctions. 180379 novel splicing junctions.
sampling 60% (172527247) splicing reads. 386848 splicing junctions. 203805 known splicing junctions. 183043 novel splicing junctions.
sampling 65% (186904518) splicing reads. 389491 splicing junctions. 204131 known splicing junctions. 185360 novel splicing junctions.
sampling 70% (201281789) splicing reads. 391699 splicing junctions. 204396 known splicing junctions. 187303 novel splicing junctions.
sampling 75% (215659059) splicing reads. 393629 splicing junctions. 204637 known splicing junctions. 188992 novel splicing junctions.
sampling 80% (230036330) splicing reads. 395159 splicing junctions. 204813 known splicing junctions. 190346 novel splicing junctions.
sampling 85% (244413601) splicing reads. 396400 splicing junctions. 204963 known splicing junctions. 191437 novel splicing junctions.
sampling 90% (258790871) splicing reads. 397360 splicing junctions. 205077 known splicing junctions. 192283 novel splicing junctions.
sampling 95% (273168142) splicing reads. 398118 splicing junctions. 205156 known splicing junctions. 192962 novel splicing junctions.
sampling 100% (287545413) splicing reads. 398636 splicing junctions. 205208 known splicing junctions. 193428 novel splicing junctions.
null device 
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