#!/bin/bash -euo pipefail
python -u /usr/local/src/CTAT-SPLICING/STAR_to_cancer_introns.py \
--SJ_tab_file tih_rna_sample_00731_B23MVV7LT4_1.SJ.out.tab \
--chimJ_file tih_rna_sample_00731_B23MVV7LT4_1.Chimeric.out.junction \
--bam_file tih_rna_sample_00731_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam \
--output_prefix tih_rna_sample_00731_B23MVV7LT4_1 \
--ctat_genome_lib ctat_genome_lib_build_dir \
--vis --sample_name tih_rna_sample_00731_B23MVV7LT4_1
# Sort output files to ensure consistent ordering across runs while preserving headers
if [ -f tih_rna_sample_00731_B23MVV7LT4_1.cancer.introns ]; then
# Extract header (first line) and sort data lines by fourth column (uniq_mapped) descending, then by first column (intron)
head -n 1 tih_rna_sample_00731_B23MVV7LT4_1.cancer.introns > tih_rna_sample_00731_B23MVV7LT4_1.cancer.introns.tmp
tail -n +2 tih_rna_sample_00731_B23MVV7LT4_1.cancer.introns | LC_ALL=C sort -k4,4nr -k1,1 >> tih_rna_sample_00731_B23MVV7LT4_1.cancer.introns.tmp
mv tih_rna_sample_00731_B23MVV7LT4_1.cancer.introns.tmp tih_rna_sample_00731_B23MVV7LT4_1.cancer.introns
fi
cat <<-END_VERSIONS > versions.yml
"NFCORE_RNAFUSION:RNAFUSION:CTATSPLICING_STARTOCANCERINTRONS":
ctat-splicing: 0.0.3
END_VERSIONS