Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/95/7d689d02309ab4996dad6f89d912a0/FFPE_V4_0002_RNA_0001_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f8/e88fd7fc7045f143707b3a331bf8bc/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e7/4c0dd4a590bb847e5bfb6f72ec5e03/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e7/4c0dd4a590bb847e5bfb6f72ec5e03/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/09/14a19fb5f4ba677d8c6d6065a18b8f/FFPE_V4_0002_RNA_0001_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: FFPE_V4_0002_RNA_0001_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (11974908) splicing reads. 218584 splicing junctions. 155384 known splicing junctions. 63200 novel splicing junctions.
sampling 10% (23949816) splicing reads. 266917 splicing junctions. 167470 known splicing junctions. 99447 novel splicing junctions.
sampling 15% (35924724) splicing reads. 300546 splicing junctions. 174067 known splicing junctions. 126479 novel splicing junctions.
sampling 20% (47899632) splicing reads. 326183 splicing junctions. 178478 known splicing junctions. 147705 novel splicing junctions.
sampling 25% (59874540) splicing reads. 347151 splicing junctions. 181657 known splicing junctions. 165494 novel splicing junctions.
sampling 30% (71849448) splicing reads. 364724 splicing junctions. 184156 known splicing junctions. 180568 novel splicing junctions.
sampling 35% (83824356) splicing reads. 379624 splicing junctions. 186136 known splicing junctions. 193488 novel splicing junctions.
sampling 40% (95799264) splicing reads. 392594 splicing junctions. 187824 known splicing junctions. 204770 novel splicing junctions.
sampling 45% (107774172) splicing reads. 403831 splicing junctions. 189144 known splicing junctions. 214687 novel splicing junctions.
sampling 50% (119749081) splicing reads. 413682 splicing junctions. 190302 known splicing junctions. 223380 novel splicing junctions.
sampling 55% (131723989) splicing reads. 422461 splicing junctions. 191247 known splicing junctions. 231214 novel splicing junctions.
sampling 60% (143698897) splicing reads. 430166 splicing junctions. 192045 known splicing junctions. 238121 novel splicing junctions.
sampling 65% (155673805) splicing reads. 436868 splicing junctions. 192789 known splicing junctions. 244079 novel splicing junctions.
sampling 70% (167648713) splicing reads. 442681 splicing junctions. 193425 known splicing junctions. 249256 novel splicing junctions.
sampling 75% (179623621) splicing reads. 447965 splicing junctions. 194019 known splicing junctions. 253946 novel splicing junctions.
sampling 80% (191598529) splicing reads. 452528 splicing junctions. 194482 known splicing junctions. 258046 novel splicing junctions.
sampling 85% (203573437) splicing reads. 456520 splicing junctions. 194923 known splicing junctions. 261597 novel splicing junctions.
sampling 90% (215548345) splicing reads. 460054 splicing junctions. 195252 known splicing junctions. 264802 novel splicing junctions.
sampling 95% (227523253) splicing reads. 463119 splicing junctions. 195552 known splicing junctions. 267567 novel splicing junctions.
sampling 100% (239498162) splicing reads. 465668 splicing junctions. 195817 known splicing junctions. 269851 novel splicing junctions.
null device 
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