[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00722_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (4902773) splicing reads. 186104 splicing junctions. 157319 known splicing junctions. 28785 novel splicing junctions.
sampling 10% (9805547) splicing reads. 208772 splicing junctions. 166611 known splicing junctions. 42161 novel splicing junctions.
sampling 15% (14708320) splicing reads. 220881 splicing junctions. 170766 known splicing junctions. 50115 novel splicing junctions.
sampling 20% (19611094) splicing reads. 228819 splicing junctions. 173168 known splicing junctions. 55651 novel splicing junctions.
sampling 25% (24513867) splicing reads. 234629 splicing junctions. 174887 known splicing junctions. 59742 novel splicing junctions.
sampling 30% (29416641) splicing reads. 238755 splicing junctions. 175971 known splicing junctions. 62784 novel splicing junctions.
sampling 35% (34319414) splicing reads. 241918 splicing junctions. 176785 known splicing junctions. 65133 novel splicing junctions.
sampling 40% (39222188) splicing reads. 244435 splicing junctions. 177407 known splicing junctions. 67028 novel splicing junctions.
sampling 45% (44124961) splicing reads. 246429 splicing junctions. 177889 known splicing junctions. 68540 novel splicing junctions.
sampling 50% (49027735) splicing reads. 248141 splicing junctions. 178283 known splicing junctions. 69858 novel splicing junctions.
sampling 55% (53930509) splicing reads. 249485 splicing junctions. 178605 known splicing junctions. 70880 novel splicing junctions.
sampling 60% (58833282) splicing reads. 250604 splicing junctions. 178886 known splicing junctions. 71718 novel splicing junctions.
sampling 65% (63736056) splicing reads. 251572 splicing junctions. 179092 known splicing junctions. 72480 novel splicing junctions.
sampling 70% (68638829) splicing reads. 252319 splicing junctions. 179243 known splicing junctions. 73076 novel splicing junctions.
sampling 75% (73541603) splicing reads. 252972 splicing junctions. 179372 known splicing junctions. 73600 novel splicing junctions.
sampling 80% (78444376) splicing reads. 253497 splicing junctions. 179485 known splicing junctions. 74012 novel splicing junctions.
sampling 85% (83347150) splicing reads. 253907 splicing junctions. 179575 known splicing junctions. 74332 novel splicing junctions.
sampling 90% (88249923) splicing reads. 254219 splicing junctions. 179624 known splicing junctions. 74595 novel splicing junctions.
sampling 95% (93152697) splicing reads. 254396 splicing junctions. 179658 known splicing junctions. 74738 novel splicing junctions.
sampling 100% (98055471) splicing reads. 254487 splicing junctions. 179680 known splicing junctions. 74807 novel splicing junctions.