[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00588_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (15841446) splicing reads. 271830 splicing junctions. 192826 known splicing junctions. 79004 novel splicing junctions.
sampling 10% (31682892) splicing reads. 331750 splicing junctions. 205461 known splicing junctions. 126289 novel splicing junctions.
sampling 15% (47524338) splicing reads. 374892 splicing junctions. 212437 known splicing junctions. 162455 novel splicing junctions.
sampling 20% (63365785) splicing reads. 408184 splicing junctions. 216752 known splicing junctions. 191432 novel splicing junctions.
sampling 25% (79207231) splicing reads. 435444 splicing junctions. 220032 known splicing junctions. 215412 novel splicing junctions.
sampling 30% (95048677) splicing reads. 458251 splicing junctions. 222432 known splicing junctions. 235819 novel splicing junctions.
sampling 35% (110890124) splicing reads. 477783 splicing junctions. 224359 known splicing junctions. 253424 novel splicing junctions.
sampling 40% (126731570) splicing reads. 494813 splicing junctions. 225966 known splicing junctions. 268847 novel splicing junctions.
sampling 45% (142573016) splicing reads. 509519 splicing junctions. 227248 known splicing junctions. 282271 novel splicing junctions.
sampling 50% (158414463) splicing reads. 522577 splicing junctions. 228302 known splicing junctions. 294275 novel splicing junctions.
sampling 55% (174255909) splicing reads. 533615 splicing junctions. 229202 known splicing junctions. 304413 novel splicing junctions.
sampling 60% (190097355) splicing reads. 543527 splicing junctions. 229957 known splicing junctions. 313570 novel splicing junctions.
sampling 65% (205938801) splicing reads. 552067 splicing junctions. 230678 known splicing junctions. 321389 novel splicing junctions.
sampling 70% (221780248) splicing reads. 559765 splicing junctions. 231282 known splicing junctions. 328483 novel splicing junctions.
sampling 75% (237621694) splicing reads. 566353 splicing junctions. 231750 known splicing junctions. 334603 novel splicing junctions.
sampling 80% (253463140) splicing reads. 571989 splicing junctions. 232153 known splicing junctions. 339836 novel splicing junctions.
sampling 85% (269304587) splicing reads. 576850 splicing junctions. 232491 known splicing junctions. 344359 novel splicing junctions.
sampling 90% (285146033) splicing reads. 580956 splicing junctions. 232780 known splicing junctions. 348176 novel splicing junctions.
sampling 95% (300987479) splicing reads. 584305 splicing junctions. 233018 known splicing junctions. 351287 novel splicing junctions.
sampling 100% (316828926) splicing reads. 586887 splicing junctions. 233237 known splicing junctions. 353650 novel splicing junctions.