Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f8/e88fd7fc7045f143707b3a331bf8bc/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/0b/d63245222fc94e9d1ac748b3df4952/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a6/f65016ba237998926cb8c2773f2ba9/tih_rna_sample_00574_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/0b/d63245222fc94e9d1ac748b3df4952/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/36/6ca63cda42c9a8f48b82024fc6f569/tih_rna_sample_00574_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00574_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (17533464) splicing reads. 294761 splicing junctions. 194685 known splicing junctions. 100076 novel splicing junctions.
sampling 10% (35066929) splicing reads. 369577 splicing junctions. 207510 known splicing junctions. 162067 novel splicing junctions.
sampling 15% (52600394) splicing reads. 424061 splicing junctions. 214428 known splicing junctions. 209633 novel splicing junctions.
sampling 20% (70133859) splicing reads. 467890 splicing junctions. 218976 known splicing junctions. 248914 novel splicing junctions.
sampling 25% (87667324) splicing reads. 503992 splicing junctions. 222093 known splicing junctions. 281899 novel splicing junctions.
sampling 30% (105200789) splicing reads. 534909 splicing junctions. 224595 known splicing junctions. 310314 novel splicing junctions.
sampling 35% (122734254) splicing reads. 561665 splicing junctions. 226582 known splicing junctions. 335083 novel splicing junctions.
sampling 40% (140267719) splicing reads. 584508 splicing junctions. 228169 known splicing junctions. 356339 novel splicing junctions.
sampling 45% (157801184) splicing reads. 605000 splicing junctions. 229496 known splicing junctions. 375504 novel splicing junctions.
sampling 50% (175334649) splicing reads. 622847 splicing junctions. 230622 known splicing junctions. 392225 novel splicing junctions.
sampling 55% (192868114) splicing reads. 638649 splicing junctions. 231560 known splicing junctions. 407089 novel splicing junctions.
sampling 60% (210401579) splicing reads. 652607 splicing junctions. 232389 known splicing junctions. 420218 novel splicing junctions.
sampling 65% (227935044) splicing reads. 664937 splicing junctions. 233100 known splicing junctions. 431837 novel splicing junctions.
sampling 70% (245468509) splicing reads. 675831 splicing junctions. 233715 known splicing junctions. 442116 novel splicing junctions.
sampling 75% (263001974) splicing reads. 685222 splicing junctions. 234210 known splicing junctions. 451012 novel splicing junctions.
sampling 80% (280535439) splicing reads. 693503 splicing junctions. 234691 known splicing junctions. 458812 novel splicing junctions.
sampling 85% (298068904) splicing reads. 700569 splicing junctions. 235061 known splicing junctions. 465508 novel splicing junctions.
sampling 90% (315602369) splicing reads. 706373 splicing junctions. 235376 known splicing junctions. 470997 novel splicing junctions.
sampling 95% (333135834) splicing reads. 711244 splicing junctions. 235652 known splicing junctions. 475592 novel splicing junctions.
sampling 100% (350669299) splicing reads. 715293 splicing junctions. 235864 known splicing junctions. 479429 novel splicing junctions.
null device 
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