Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/38/1795f1b93f908361dff2828332edf0/tih_rna_sample_00709_B23MVV7LT4_1.Chimeric.out.junction Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/76/b7ef0e062fb3205a130605370a7746/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/38/1795f1b93f908361dff2828332edf0/tih_rna_sample_00709_B23MVV7LT4_1.SJ.out.tab Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/38/1795f1b93f908361dff2828332edf0/tih_rna_sample_00709_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/c1/96eb7966c49927a6354f93724f837c/tih_rna_sample_00709_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/76/b7ef0e062fb3205a130605370a7746/.command.run ==> STAGING COMPLETE (7 inputs) 08:35:53 : INFO 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rate=23611764.71/min [13390000], rate=23629411.76/min [13400000], rate=23647058.82/min [13410000], rate=23664705.88/min [13420000], rate=23682352.94/min [13430000], rate=23700000.00/min [13440000], rate=23717647.06/min [13450000], rate=23735294.12/min [13460000], rate=23752941.18/min [13470000], rate=23770588.24/min [13480000], rate=23788235.29/min [13490000], rate=23805882.35/min [13500000], rate=23823529.41/min [13510000], rate=23841176.47/min [13520000], rate=23858823.53/min [13530000], rate=23876470.59/min [13540000], rate=23894117.65/min [13550000], rate=23911764.71/min [13560000], rate=23929411.76/min [13570000], rate=23947058.82/min [13580000], rate=23964705.88/min [13590000], rate=23982352.94/min [13600000], rate=24000000.00/min [13610000], rate=24017647.06/min [13620000], rate=24035294.12/min [13630000], rate=24052941.18/min [13640000], rate=24070588.24/min [13650000], rate=24088235.29/min [13660000], rate=24105882.35/min [13670000], rate=23434285.71/min [13680000], rate=23451428.57/min [13690000], rate=23468571.43/min [13700000], rate=23485714.29/min [13710000], rate=23502857.14/min [13720000], rate=23520000.00/min [13730000], rate=23537142.86/min [13740000], rate=23554285.71/min [13750000], rate=23571428.57/min [13760000], rate=23588571.43/min [13770000], rate=23605714.29/min [13780000], rate=23622857.14/min [13790000], rate=23640000.00/min [13800000], rate=23657142.86/min [13810000], rate=23674285.71/min [13820000], rate=23691428.57/min [13830000], rate=23708571.43/min [13840000], rate=23725714.29/min [13850000], rate=23742857.14/min [13860000], rate=23760000.00/min [13870000], rate=23777142.86/min [13880000], rate=23794285.71/min [13890000], rate=23811428.57/min [13900000], rate=23828571.43/min [13910000], rate=23845714.29/min [13920000], rate=23862857.14/min [13930000], rate=23880000.00/min [13940000], rate=23897142.86/min [13950000], rate=23914285.71/min [13960000], rate=23931428.57/min [13970000], rate=23948571.43/min [13980000], rate=23965714.29/min [13990000], rate=23982857.14/min [14000000], rate=24000000.00/min [14010000], rate=24017142.86/min [14020000], rate=24034285.71/min [14030000], rate=24051428.57/min [14040000], rate=24068571.43/min [14050000], rate=24085714.29/min [14060000], rate=24102857.14/min [14070000], rate=24120000.00/min [14080000], rate=24137142.86/min [14090000], rate=24154285.71/min [14100000], rate=24171428.57/min [14110000], rate=23516666.67/min [14120000], rate=23533333.33/min [14130000], rate=23550000.00/min [14140000], rate=23566666.67/min [14150000], rate=23583333.33/min [14160000], rate=23600000.00/min [14170000], rate=23616666.67/min [14180000], rate=23633333.33/min [14190000], rate=23650000.00/min [14200000], rate=23666666.67/min [14210000], rate=23683333.33/min [14220000], rate=23700000.00/min [14230000], rate=23716666.67/min [14240000], rate=23733333.33/min [14250000], rate=23750000.00/min [14260000], rate=23766666.67/min [14270000], rate=23783333.33/min [14280000], rate=23800000.00/min [14290000], rate=23816666.67/min [14300000], rate=23833333.33/min [14310000], rate=23850000.00/min [14320000], rate=23866666.67/min [14330000], rate=23883333.33/min [14340000], rate=23900000.00/min [14350000], rate=23916666.67/min [14360000], rate=23933333.33/min [14370000], rate=23950000.00/min [14380000], rate=23966666.67/min [14390000], rate=23983333.33/min [14400000], rate=24000000.00/min [14410000], rate=24016666.67/min [14420000], rate=24033333.33/min [14430000], rate=24050000.00/min [14440000], rate=24066666.67/min 08:36:37 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file tih_rna_sample_00709_B23MVV7LT4_1.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > tih_rna_sample_00709_B23MVV7LT4_1.cancer.introns.prelim Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18. -tih_rna_sample_00709_B23MVV7LT4_1.introns: identified 2892 cancer introns 08:36:38 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file tih_rna_sample_00709_B23MVV7LT4_1.introns --ctat_genome_lib ctat_genome_lib_build_dir --intron_col 0 > tih_rna_sample_00709_B23MVV7LT4_1.cancer.introns.prelim 08:36:38 : INFO : Running: touch /tmp/nxf.MyKHte4niV/tih_rna_sample_00709_B23MVV7LT4_1.chckpts/prelim_introns.ok 08:36:38 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates tih_rna_sample_00709_B23MVV7LT4_1.cancer.introns.prelim --min_total_reads 5 > tih_rna_sample_00709_B23MVV7LT4_1.cancer.introns 08:36:38 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates tih_rna_sample_00709_B23MVV7LT4_1.cancer.introns.prelim --min_total_reads 5 > tih_rna_sample_00709_B23MVV7LT4_1.cancer.introns 08:36:38 : INFO : Running: touch /tmp/nxf.MyKHte4niV/tih_rna_sample_00709_B23MVV7LT4_1.chckpts/introns_filtered.ok 08:36:38 : INFO : -found 1744 cancer introns 08:36:38 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns tih_rna_sample_00709_B23MVV7LT4_1.introns --cancer_introns tih_rna_sample_00709_B23MVV7LT4_1.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed tih_rna_sample_00709_B23MVV7LT4_1.introns.for_IGV.bed 08:36:38 : INFO : Creating the BED File. 08:36:42 : INFO : Saving Bed File as tih_rna_sample_00709_B23MVV7LT4_1.introns.for_IGV.bed 08:36:42 : INFO : Execution Time = 0.06 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns tih_rna_sample_00709_B23MVV7LT4_1.introns --cancer_introns tih_rna_sample_00709_B23MVV7LT4_1.cancer.introns --genome_lib_dir ctat_genome_lib_build_dir --output_bed tih_rna_sample_00709_B23MVV7LT4_1.introns.for_IGV.bed 08:36:42 : INFO : Running: touch /tmp/nxf.MyKHte4niV/tih_rna_sample_00709_B23MVV7LT4_1.chckpts/intron_igv_bed.ok 08:36:42 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed tih_rna_sample_00709_B23MVV7LT4_1.introns.for_IGV.bed --bam tih_rna_sample_00709_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam --output_prefix tih_rna_sample_00709_B23MVV7LT4_1 [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00709_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai 08:51:09 : INFO : Execution Time = 14.46 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed tih_rna_sample_00709_B23MVV7LT4_1.introns.for_IGV.bed --bam tih_rna_sample_00709_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam --output_prefix tih_rna_sample_00709_B23MVV7LT4_1 08:51:09 : INFO : Running: touch /tmp/nxf.MyKHte4niV/tih_rna_sample_00709_B23MVV7LT4_1.chckpts/reads_alignments_extracted.ok 08:51:09 : INFO : Running: samtools sort -o tih_rna_sample_00709_B23MVV7LT4_1.cancer_intron_reads.sorted.bam tih_rna_sample_00709_B23MVV7LT4_1.cancer_intron_reads.bam 08:51:10 : INFO : Execution Time = 0.00 minutes. CMD: samtools sort -o tih_rna_sample_00709_B23MVV7LT4_1.cancer_intron_reads.sorted.bam tih_rna_sample_00709_B23MVV7LT4_1.cancer_intron_reads.bam 08:51:10 : INFO : Running: touch /tmp/nxf.MyKHte4niV/tih_rna_sample_00709_B23MVV7LT4_1.chckpts/sort_cancer_intron_reads.ok 08:51:10 : INFO : Running: samtools sort -o tih_rna_sample_00709_B23MVV7LT4_1.gene_reads.sorted.bam tih_rna_sample_00709_B23MVV7LT4_1.gene_reads.bam [bam_sort_core] merging from 90 files and 1 in-memory blocks...