[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00586_B23MVV7LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (10185752) splicing reads. 238759 splicing junctions. 178868 known splicing junctions. 59891 novel splicing junctions.
sampling 10% (20371504) splicing reads. 287572 splicing junctions. 191770 known splicing junctions. 95802 novel splicing junctions.
sampling 15% (30557256) splicing reads. 322181 splicing junctions. 198885 known splicing junctions. 123296 novel splicing junctions.
sampling 20% (40743009) splicing reads. 349196 splicing junctions. 203679 known splicing junctions. 145517 novel splicing junctions.
sampling 25% (50928761) splicing reads. 371433 splicing junctions. 207114 known splicing junctions. 164319 novel splicing junctions.
sampling 30% (61114513) splicing reads. 390122 splicing junctions. 209688 known splicing junctions. 180434 novel splicing junctions.
sampling 35% (71300265) splicing reads. 406286 splicing junctions. 211803 known splicing junctions. 194483 novel splicing junctions.
sampling 40% (81486018) splicing reads. 420260 splicing junctions. 213521 known splicing junctions. 206739 novel splicing junctions.
sampling 45% (91671770) splicing reads. 432565 splicing junctions. 214973 known splicing junctions. 217592 novel splicing junctions.
sampling 50% (101857522) splicing reads. 443371 splicing junctions. 216208 known splicing junctions. 227163 novel splicing junctions.
sampling 55% (112043274) splicing reads. 452747 splicing junctions. 217191 known splicing junctions. 235556 novel splicing junctions.
sampling 60% (122229027) splicing reads. 461235 splicing junctions. 218052 known splicing junctions. 243183 novel splicing junctions.
sampling 65% (132414779) splicing reads. 468833 splicing junctions. 218829 known splicing junctions. 250004 novel splicing junctions.
sampling 70% (142600531) splicing reads. 475566 splicing junctions. 219542 known splicing junctions. 256024 novel splicing junctions.
sampling 75% (152786283) splicing reads. 481550 splicing junctions. 220168 known splicing junctions. 261382 novel splicing junctions.
sampling 80% (162972036) splicing reads. 486928 splicing junctions. 220693 known splicing junctions. 266235 novel splicing junctions.
sampling 85% (173157788) splicing reads. 491587 splicing junctions. 221119 known splicing junctions. 270468 novel splicing junctions.
sampling 90% (183343540) splicing reads. 495646 splicing junctions. 221532 known splicing junctions. 274114 novel splicing junctions.
sampling 95% (193529292) splicing reads. 498997 splicing junctions. 221830 known splicing junctions. 277167 novel splicing junctions.
sampling 100% (203715045) splicing reads. 501917 splicing junctions. 222096 known splicing junctions. 279821 novel splicing junctions.