FastQCFastQC Report
Mon 8 Jun 2026
NTC_0001_0001_B23WHYVLT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_B23WHYVLT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959867
Total Bases144.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC73

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCTGGT44541446.40372051544641TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCTGGG22461923.401054521095112TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCTAGT14978815.605078620267182TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCTAGG466374.858693964893053TruSeq Adapter, Index 4 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCTGGTG45720.4763159896110607TruSeq Adapter, Index 4 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCTGGGG36270.3778648500260974TruSeq Adapter, Index 4 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCTGGTA28960.3017084658603744TruSeq Adapter, Index 4 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCTGGTGTG28610.2980621273572276TruSeq Adapter, Index 4 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCTGGTATG20670.21534233388584043TruSeq Adapter, Index 4 (97% over 34bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA19960.20794547577945693No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT19600.20419495617622027No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATGTGGG15400.16043889413845877TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCGGGG15280.15918872093737987TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATTTGGT15040.15668837453522208TruSeq Adapter, Index 4 (97% over 37bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC13990.1457493590257817No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCGGGT12780.1331434459149028TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATGTGGT12630.13158072941355417TruSeq Adapter, Index 4 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCTGGGGTG12490.13012219401229544TruSeq Adapter, Index 4 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATTTGGG12190.1269967610095982TruSeq Adapter, Index 4 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACTAGATGATCTAGTA12150.12658003660923856TruSeq Adapter, Index 4 (97% over 36bp)
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG11880.12376714690681104No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)