[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-f71b32-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	327905011
Known Splicing Events:	324872858
Partial Novel Splicing Events:	1948288
Novel Splicing Events:	987755
Filtered Splicing Events:	96110

Total splicing  Junctions:	600558
Known Splicing Junctions:	288319
Partial Novel Splicing Junctions:	182862
Novel Splicing Junctions:	129377

===================================================================
Create BED file ...
Create Interact file ...