+ mkdir aih-tih-sc-c7b68f-R1_B23WHYVLT4_1 + all_qc -bam aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-c7b68f-R1_B23WHYVLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai 0it [00:00, ?it/s] 105489it [00:00, 1054810.53it/s] 246516it [00:00, 1263854.25it/s] 398643it [00:00, 1381333.46it/s] 560067it [00:00, 1473241.14it/s] 707392it [00:00, 1452271.86it/s] 856540it [00:00, 1465438.04it/s] 1003131it [00:00, 1452275.20it/s] 1164139it [00:00, 1501952.52it/s] 1317993it [00:00, 1513166.12it/s] 1477486it [00:01, 1538233.16it/s] 1631369it [00:01, 1381503.69it/s] 1772471it [00:01, 1372925.55it/s] 1911775it [00:01, 1331798.63it/s] 2072379it [00:01, 1409002.27it/s] 2214868it [00:01, 1354417.01it/s] 2352060it [00:01, 1359225.82it/s] 2515214it [00:01, 1437391.35it/s] 2673924it [00:01, 1480877.37it/s] 2823171it [00:01, 1484078.19it/s] 2979603it [00:02, 1507758.40it/s] 3149655it [00:02, 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[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md.bai + merge_qc -folders aih-tih-sc-c7b68f-R1_B23WHYVLT4_1/aih-tih-sc-c7b68f-R1_B23WHYVLT4_1.md -output aih-tih-sc-c7b68f-R1_B23WHYVLT4_1/aih-tih-sc-c7b68f-R1_B23WHYVLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-c7b68f-R1_B23WHYVLT4_1/aih-tih-sc-c7b68f-R1_B23WHYVLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-c7b68f-R1_B23WHYVLT4_1/aih-tih-sc-c7b68f-R1_B23WHYVLT4_1_full_basic_stats.tsv