[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b3c1e-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference gene model ref_annot.bed ... Done
Loading SAM/BAM file ...  Total 200000 usable reads were sampled