[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3376ed-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (18059874) splicing reads. 268244 splicing junctions. 180643 known splicing junctions. 87601 novel splicing junctions.
sampling 10% (36119749) splicing reads. 332444 splicing junctions. 193334 known splicing junctions. 139110 novel splicing junctions.
sampling 15% (54179624) splicing reads. 377948 splicing junctions. 200221 known splicing junctions. 177727 novel splicing junctions.
sampling 20% (72239499) splicing reads. 413356 splicing junctions. 204687 known splicing junctions. 208669 novel splicing junctions.
sampling 25% (90299374) splicing reads. 442233 splicing junctions. 207819 known splicing junctions. 234414 novel splicing junctions.
sampling 30% (108359249) splicing reads. 466615 splicing junctions. 210271 known splicing junctions. 256344 novel splicing junctions.
sampling 35% (126419123) splicing reads. 487276 splicing junctions. 212244 known splicing junctions. 275032 novel splicing junctions.
sampling 40% (144478998) splicing reads. 504901 splicing junctions. 213779 known splicing junctions. 291122 novel splicing junctions.
sampling 45% (162538873) splicing reads. 520178 splicing junctions. 215115 known splicing junctions. 305063 novel splicing junctions.
sampling 50% (180598748) splicing reads. 533570 splicing junctions. 216163 known splicing junctions. 317407 novel splicing junctions.
sampling 55% (198658623) splicing reads. 545322 splicing junctions. 217037 known splicing junctions. 328285 novel splicing junctions.
sampling 60% (216718498) splicing reads. 555776 splicing junctions. 217827 known splicing junctions. 337949 novel splicing junctions.
sampling 65% (234778373) splicing reads. 564802 splicing junctions. 218500 known splicing junctions. 346302 novel splicing junctions.
sampling 70% (252838247) splicing reads. 572744 splicing junctions. 219101 known splicing junctions. 353643 novel splicing junctions.
sampling 75% (270898122) splicing reads. 579664 splicing junctions. 219551 known splicing junctions. 360113 novel splicing junctions.
sampling 80% (288957997) splicing reads. 585791 splicing junctions. 219981 known splicing junctions. 365810 novel splicing junctions.
sampling 85% (307017872) splicing reads. 590961 splicing junctions. 220311 known splicing junctions. 370650 novel splicing junctions.
sampling 90% (325077747) splicing reads. 595333 splicing junctions. 220616 known splicing junctions. 374717 novel splicing junctions.
sampling 95% (343137622) splicing reads. 598978 splicing junctions. 220864 known splicing junctions. 378114 novel splicing junctions.
sampling 100% (361197497) splicing reads. 601950 splicing junctions. 221049 known splicing junctions. 380901 novel splicing junctions.