+ mkdir aih-tih-sc-4dcd88-R1_B23WHYVLT4_1 + all_qc -bam aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-4dcd88-R1_B23WHYVLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai 0it [00:00, ?it/s] 85196it [00:00, 851802.41it/s] 184356it [00:00, 933992.56it/s] 280696it [00:00, 947394.40it/s] 388212it [00:00, 997812.73it/s] 521373it [00:00, 1118059.88it/s] 656385it [00:00, 1196929.70it/s] 798969it [00:00, 1271739.44it/s] 949132it [00:00, 1344898.80it/s] 1112134it [00:00, 1433944.04it/s] 1259975it [00:01, 1447648.57it/s] 1428700it [00:01, 1520950.22it/s] 1597986it [00:01, 1573132.70it/s] 1768146it [00:01, 1611924.74it/s] 1933515it [00:01, 1624516.67it/s] 2095967it [00:01, 1513081.27it/s] 2259061it [00:01, 1546782.29it/s] 2427031it [00:01, 1585329.06it/s] 2598329it [00:01, 1622707.75it/s] 2761399it [00:01, 1614808.99it/s] 2923437it [00:02, 1615536.55it/s] 3096501it [00:02, 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1541441.17it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai 17081457it [00:11, 1580600.92it/s] 17239673it [00:11, 1576587.28it/s] 17397441it [00:11, 1533830.35it/s] 17552216it [00:11, 1537854.36it/s] 17674056it [00:11, 1531361.51it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md.bai + merge_qc -folders aih-tih-sc-4dcd88-R1_B23WHYVLT4_1/aih-tih-sc-4dcd88-R1_B23WHYVLT4_1.md -output aih-tih-sc-4dcd88-R1_B23WHYVLT4_1/aih-tih-sc-4dcd88-R1_B23WHYVLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-4dcd88-R1_B23WHYVLT4_1/aih-tih-sc-4dcd88-R1_B23WHYVLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-4dcd88-R1_B23WHYVLT4_1/aih-tih-sc-4dcd88-R1_B23WHYVLT4_1_full_basic_stats.tsv