[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-1d89ab-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (15642203) splicing reads. 263283 splicing junctions. 185707 known splicing junctions. 77576 novel splicing junctions.
sampling 10% (31284406) splicing reads. 318572 splicing junctions. 198079 known splicing junctions. 120493 novel splicing junctions.
sampling 15% (46926609) splicing reads. 355392 splicing junctions. 204425 known splicing junctions. 150967 novel splicing junctions.
sampling 20% (62568813) splicing reads. 383043 splicing junctions. 208624 known splicing junctions. 174419 novel splicing junctions.
sampling 25% (78211016) splicing reads. 404607 splicing junctions. 211470 known splicing junctions. 193137 novel splicing junctions.
sampling 30% (93853219) splicing reads. 422042 splicing junctions. 213576 known splicing junctions. 208466 novel splicing junctions.
sampling 35% (109495422) splicing reads. 436619 splicing junctions. 215220 known splicing junctions. 221399 novel splicing junctions.
sampling 40% (125137626) splicing reads. 448769 splicing junctions. 216586 known splicing junctions. 232183 novel splicing junctions.
sampling 45% (140779829) splicing reads. 459155 splicing junctions. 217743 known splicing junctions. 241412 novel splicing junctions.
sampling 50% (156422032) splicing reads. 467917 splicing junctions. 218637 known splicing junctions. 249280 novel splicing junctions.
sampling 55% (172064235) splicing reads. 475602 splicing junctions. 219399 known splicing junctions. 256203 novel splicing junctions.
sampling 60% (187706439) splicing reads. 482182 splicing junctions. 220052 known splicing junctions. 262130 novel splicing junctions.
sampling 65% (203348642) splicing reads. 487809 splicing junctions. 220603 known splicing junctions. 267206 novel splicing junctions.
sampling 70% (218990845) splicing reads. 492536 splicing junctions. 221046 known splicing junctions. 271490 novel splicing junctions.
sampling 75% (234633048) splicing reads. 496548 splicing junctions. 221443 known splicing junctions. 275105 novel splicing junctions.
sampling 80% (250275252) splicing reads. 499960 splicing junctions. 221785 known splicing junctions. 278175 novel splicing junctions.
sampling 85% (265917455) splicing reads. 502901 splicing junctions. 222039 known splicing junctions. 280862 novel splicing junctions.
sampling 90% (281559658) splicing reads. 505209 splicing junctions. 222232 known splicing junctions. 282977 novel splicing junctions.
sampling 95% (297201861) splicing reads. 506949 splicing junctions. 222397 known splicing junctions. 284552 novel splicing junctions.
sampling 100% (312844065) splicing reads. 508238 splicing junctions. 222513 known splicing junctions. 285725 novel splicing junctions.