[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-20b7e4-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (4474729) splicing reads. 180330 splicing junctions. 153837 known splicing junctions. 26493 novel splicing junctions.
sampling 10% (8949459) splicing reads. 203745 splicing junctions. 164540 known splicing junctions. 39205 novel splicing junctions.
sampling 15% (13424188) splicing reads. 216660 splicing junctions. 169373 known splicing junctions. 47287 novel splicing junctions.
sampling 20% (17898918) splicing reads. 225046 splicing junctions. 172170 known splicing junctions. 52876 novel splicing junctions.
sampling 25% (22373647) splicing reads. 230933 splicing junctions. 173969 known splicing junctions. 56964 novel splicing junctions.
sampling 30% (26848377) splicing reads. 235375 splicing junctions. 175348 known splicing junctions. 60027 novel splicing junctions.
sampling 35% (31323106) splicing reads. 238820 splicing junctions. 176308 known splicing junctions. 62512 novel splicing junctions.
sampling 40% (35797836) splicing reads. 241557 splicing junctions. 177046 known splicing junctions. 64511 novel splicing junctions.
sampling 45% (40272565) splicing reads. 243851 splicing junctions. 177643 known splicing junctions. 66208 novel splicing junctions.
sampling 50% (44747295) splicing reads. 245692 splicing junctions. 178129 known splicing junctions. 67563 novel splicing junctions.
sampling 55% (49222024) splicing reads. 247187 splicing junctions. 178504 known splicing junctions. 68683 novel splicing junctions.
sampling 60% (53696754) splicing reads. 248491 splicing junctions. 178839 known splicing junctions. 69652 novel splicing junctions.
sampling 65% (58171483) splicing reads. 249556 splicing junctions. 179091 known splicing junctions. 70465 novel splicing junctions.
sampling 70% (62646212) splicing reads. 250425 splicing junctions. 179305 known splicing junctions. 71120 novel splicing junctions.
sampling 75% (67120942) splicing reads. 251119 splicing junctions. 179475 known splicing junctions. 71644 novel splicing junctions.
sampling 80% (71595672) splicing reads. 251748 splicing junctions. 179651 known splicing junctions. 72097 novel splicing junctions.
sampling 85% (76070401) splicing reads. 252252 splicing junctions. 179781 known splicing junctions. 72471 novel splicing junctions.
sampling 90% (80545131) splicing reads. 252624 splicing junctions. 179872 known splicing junctions. 72752 novel splicing junctions.
sampling 95% (85019860) splicing reads. 252873 splicing junctions. 179933 known splicing junctions. 72940 novel splicing junctions.
sampling 100% (89494590) splicing reads. 252995 splicing junctions. 179959 known splicing junctions. 73036 novel splicing junctions.