Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/bb/013786bf60e48795a82bf865049234/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/4b/281c9e3fdc07ee3ab382a0408a703e/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/4b/281c9e3fdc07ee3ab382a0408a703e/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/40/9974715b26386cbe22e03d563a248d/aih-tih-sc-16054b-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/7a/e3f8fc649ff395e795586b9719e337/aih-tih-sc-16054b-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-16054b-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (18004677) splicing reads. 262758 splicing junctions. 187438 known splicing junctions. 75320 novel splicing junctions.
sampling 10% (36009355) splicing reads. 319406 splicing junctions. 199969 known splicing junctions. 119437 novel splicing junctions.
sampling 15% (54014032) splicing reads. 359379 splicing junctions. 206836 known splicing junctions. 152543 novel splicing junctions.
sampling 20% (72018710) splicing reads. 390656 splicing junctions. 211501 known splicing junctions. 179155 novel splicing junctions.
sampling 25% (90023388) splicing reads. 416267 splicing junctions. 214764 known splicing junctions. 201503 novel splicing junctions.
sampling 30% (108028065) splicing reads. 437566 splicing junctions. 217303 known splicing junctions. 220263 novel splicing junctions.
sampling 35% (126032743) splicing reads. 455736 splicing junctions. 219314 known splicing junctions. 236422 novel splicing junctions.
sampling 40% (144037420) splicing reads. 471145 splicing junctions. 220897 known splicing junctions. 250248 novel splicing junctions.
sampling 45% (162042098) splicing reads. 484722 splicing junctions. 222218 known splicing junctions. 262504 novel splicing junctions.
sampling 50% (180046776) splicing reads. 496940 splicing junctions. 223389 known splicing junctions. 273551 novel splicing junctions.
sampling 55% (198051453) splicing reads. 507483 splicing junctions. 224382 known splicing junctions. 283101 novel splicing junctions.
sampling 60% (216056131) splicing reads. 516732 splicing junctions. 225172 known splicing junctions. 291560 novel splicing junctions.
sampling 65% (234060808) splicing reads. 525044 splicing junctions. 225903 known splicing junctions. 299141 novel splicing junctions.
sampling 70% (252065486) splicing reads. 532220 splicing junctions. 226492 known splicing junctions. 305728 novel splicing junctions.
sampling 75% (270070164) splicing reads. 538465 splicing junctions. 227010 known splicing junctions. 311455 novel splicing junctions.
sampling 80% (288074841) splicing reads. 543876 splicing junctions. 227449 known splicing junctions. 316427 novel splicing junctions.
sampling 85% (306079519) splicing reads. 548470 splicing junctions. 227854 known splicing junctions. 320616 novel splicing junctions.
sampling 90% (324084196) splicing reads. 552404 splicing junctions. 228157 known splicing junctions. 324247 novel splicing junctions.
sampling 95% (342088874) splicing reads. 555717 splicing junctions. 228421 known splicing junctions. 327296 novel splicing junctions.
sampling 100% (360093552) splicing reads. 558226 splicing junctions. 228612 known splicing junctions. 329614 novel splicing junctions.
null device 
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