[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-070e1d-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (14095669) splicing reads. 250071 splicing junctions. 177807 known splicing junctions. 72264 novel splicing junctions.
sampling 10% (28191339) splicing reads. 295677 splicing junctions. 188592 known splicing junctions. 107085 novel splicing junctions.
sampling 15% (42287008) splicing reads. 323104 splicing junctions. 193712 known splicing junctions. 129392 novel splicing junctions.
sampling 20% (56382678) splicing reads. 342148 splicing junctions. 196886 known splicing junctions. 145262 novel splicing junctions.
sampling 25% (70478348) splicing reads. 355745 splicing junctions. 198930 known splicing junctions. 156815 novel splicing junctions.
sampling 30% (84574017) splicing reads. 366385 splicing junctions. 200510 known splicing junctions. 165875 novel splicing junctions.
sampling 35% (98669687) splicing reads. 374514 splicing junctions. 201640 known splicing junctions. 172874 novel splicing junctions.
sampling 40% (112765356) splicing reads. 381288 splicing junctions. 202562 known splicing junctions. 178726 novel splicing junctions.
sampling 45% (126861026) splicing reads. 386675 splicing junctions. 203297 known splicing junctions. 183378 novel splicing junctions.
sampling 50% (140956696) splicing reads. 391176 splicing junctions. 203868 known splicing junctions. 187308 novel splicing junctions.
sampling 55% (155052365) splicing reads. 394883 splicing junctions. 204309 known splicing junctions. 190574 novel splicing junctions.
sampling 60% (169148035) splicing reads. 397952 splicing junctions. 204709 known splicing junctions. 193243 novel splicing junctions.
sampling 65% (183243704) splicing reads. 400590 splicing junctions. 205035 known splicing junctions. 195555 novel splicing junctions.
sampling 70% (197339374) splicing reads. 402807 splicing junctions. 205282 known splicing junctions. 197525 novel splicing junctions.
sampling 75% (211435044) splicing reads. 404590 splicing junctions. 205502 known splicing junctions. 199088 novel splicing junctions.
sampling 80% (225530713) splicing reads. 405999 splicing junctions. 205672 known splicing junctions. 200327 novel splicing junctions.
sampling 85% (239626383) splicing reads. 407126 splicing junctions. 205797 known splicing junctions. 201329 novel splicing junctions.
sampling 90% (253722052) splicing reads. 407967 splicing junctions. 205904 known splicing junctions. 202063 novel splicing junctions.
sampling 95% (267817722) splicing reads. 408584 splicing junctions. 205975 known splicing junctions. 202609 novel splicing junctions.
sampling 100% (281913392) splicing reads. 408972 splicing junctions. 206018 known splicing junctions. 202954 novel splicing junctions.