[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2f84cc-R1_B23WHYVLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (5285649) splicing reads. 191487 splicing junctions. 157962 known splicing junctions. 33525 novel splicing junctions.
sampling 10% (10571299) splicing reads. 223694 splicing junctions. 170503 known splicing junctions. 53191 novel splicing junctions.
sampling 15% (15856948) splicing reads. 244937 splicing junctions. 176984 known splicing junctions. 67953 novel splicing junctions.
sampling 20% (21142598) splicing reads. 260369 splicing junctions. 181172 known splicing junctions. 79197 novel splicing junctions.
sampling 25% (26428247) splicing reads. 272447 splicing junctions. 184085 known splicing junctions. 88362 novel splicing junctions.
sampling 30% (31713897) splicing reads. 282244 splicing junctions. 186328 known splicing junctions. 95916 novel splicing junctions.
sampling 35% (36999546) splicing reads. 290209 splicing junctions. 188018 known splicing junctions. 102191 novel splicing junctions.
sampling 40% (42285196) splicing reads. 297029 splicing junctions. 189434 known splicing junctions. 107595 novel splicing junctions.
sampling 45% (47570845) splicing reads. 302857 splicing junctions. 190664 known splicing junctions. 112193 novel splicing junctions.
sampling 50% (52856495) splicing reads. 307729 splicing junctions. 191579 known splicing junctions. 116150 novel splicing junctions.
sampling 55% (58142145) splicing reads. 311857 splicing junctions. 192369 known splicing junctions. 119488 novel splicing junctions.
sampling 60% (63427794) splicing reads. 315443 splicing junctions. 193046 known splicing junctions. 122397 novel splicing junctions.
sampling 65% (68713444) splicing reads. 318472 splicing junctions. 193609 known splicing junctions. 124863 novel splicing junctions.
sampling 70% (73999093) splicing reads. 320953 splicing junctions. 194053 known splicing junctions. 126900 novel splicing junctions.
sampling 75% (79284743) splicing reads. 323146 splicing junctions. 194405 known splicing junctions. 128741 novel splicing junctions.
sampling 80% (84570392) splicing reads. 324987 splicing junctions. 194718 known splicing junctions. 130269 novel splicing junctions.
sampling 85% (89856042) splicing reads. 326463 splicing junctions. 195000 known splicing junctions. 131463 novel splicing junctions.
sampling 90% (95141691) splicing reads. 327579 splicing junctions. 195206 known splicing junctions. 132373 novel splicing junctions.
sampling 95% (100427341) splicing reads. 328445 splicing junctions. 195354 known splicing junctions. 133091 novel splicing junctions.
sampling 100% (105712991) splicing reads. 329015 splicing junctions. 195460 known splicing junctions. 133555 novel splicing junctions.